scholarly journals Efficacy of Caffeic Acid on Diabetes and Its Complications in the Mouse

Molecules ◽  
2021 ◽  
Vol 26 (11) ◽  
pp. 3262
Author(s):  
Nada Oršolić ◽  
Damir Sirovina ◽  
Dyana Odeh ◽  
Goran Gajski ◽  
Vedran Balta ◽  
...  
Keyword(s):  
Lipid Profile ◽  
Caffeic Acid ◽  
Fasting Blood Glucose ◽  
Kidney Tissue ◽  
Serum Glucose ◽  
The Body ◽  
Diabetic Mice ◽  
Atherogenic Indices ◽  
Liver And Kidney ◽  
Hematological And Biochemical Parameters

Diabetic dyslipidemia and hyperglycemia contribute to excessive reactive oxygen species (ROS) production, leading to deleterious complications, such as nephropathy, atherosclerosis and cardiac dysfunction, and target major organs in the body. The aim of this study was to investigate the effect of caffeic acid (CA) on mouse weight and survival, serum level of fasting blood glucose (FBG), serum lipid parameters and atherogenic indices, oxidative damage in blood, liver and kidney tissue, pathophysiological changes and their function markers in healthy and alloxan-induced type 1 diabetic mice. Diabetes was induced in mice with a single intravenous injection of alloxan (75 mg kg−1). Two days later, CA (50 mg kg−1) was given intraperitoneally for seven days in diabetic mice. Diabetes affected glucose level, lipid profile, hematological and biochemical parameters, induced DNA damage and apoptotic/necrotic death in whole blood cells, liver and kidney, leading to weight loss and a decreased lifespan. CA treatment of diabetic mice revealed a protective effect on the liver and kidney, hypoglycemic and hypolipidemic properties and high protection against atherogenic outcomes. The obtained results suggest that CA is a safe and potent agent against diabetes that acts as an effective antioxidant in reducing serum glucose, lipid profile and atherogenic indices, leading to increased lifespan in mice.

Anti-Diabetic Effect of a Flavonoid and Sitosterol - Rich Aqueous Extract ofPleurotus tuberregiumSclerotia in Alloxan-Induced Diabetic Rabbits

2019 ◽  
Vol 19 (8) ◽  
pp. 1148-1156 ◽  
Author(s):  
Ifeanacho Mercy Onuekwuzu ◽  
Ikewuchi Catherine Chidinma ◽  
Ikewuchi Jude Chigozie
Keyword(s):  
Diabetes Mellitus ◽  
Blood Glucose ◽  
Aqueous Extract ◽  
Risk Index ◽  
Fasting Blood Glucose ◽  
Hdl Cholesterol ◽  
The Body ◽  
Atherogenic Index ◽  
Atherogenic Indices ◽  
Diabetic Rabbits

Objective:Traditionally prepared infusions and decoctions are commonly used in the management of diabetes mellitus, in southern Nigeria; one of such is the aqueous extract of the sclerotia of Pleurotus tuberregium (“usu” milk). In this study, the effects of the extract on the body weights, tissue/ organ weights, fasting blood glucose, blood/plasma lipid profiles and atherogenic indices were investigated in normal and alloxan-induced diabetic rabbits.Methods:Diabetes mellitus was induced by the injection of alloxan (120 mg/kg body weight) via the marginal ear vein. The extract was administered orally at 100, 200 and 300 mg/kg to normal and diabetic rabbits; while metformin was administered at 50 mg/kg. The crude extract was analyzed by gas chromatography, coupled to flame ionization detector.Results:Thirty-one known flavonoids were detected, consisting mainly of isoquercetin (28.5%), luteolin (24.3%), quercetin (18.8%) and kaempferol (11.3%). Sitosterol (82.0%) and stigmasterol (12.5%) were the most abundant of the seven phytosterols detected. Compared to the diabetic control, the treatment significantly (p<0.05) lowered the weights of the kidney and liver, as well as the levels of blood glucose and triglyceride, plasma VLDL, LDL and non-HDL cholesterol, atherogenic index of plasma, cardiac risk ratio, atherogenic coefficient and Castelli’s risk index II. It, however, significantly (p<0.05) increased plasma HDL cholesterol, without significantly affecting blood total cholesterol levels.Conclusion:This study showed that the extract was hypoglycemic, and improved lipid profile and atherogenic indices, thus highlighting its cardioprotective potential, thereby supporting its use in the management of diabetes mellitus.

scholarly journals Prophylactic effect of vitamin E against hepatotoxicity, nephrotoxicity, haematological indices and histopathology induced by diazinon insecticide in mice

2009 ◽  
Vol 55 (3) ◽  
pp. 219-226 ◽  
Author(s):  
Nahla S. El-Shenawy ◽  
Rasha A. Al-Eisa ◽  
Fawzia El-Salmy ◽  
Omema Salah
Keyword(s):  
Body Weight ◽  
Vitamin E ◽  
Kidney Function ◽  
Kidney Tissue ◽  
The Body ◽  
High Dose ◽  
Protective Effects ◽  
Histopathological Changes ◽  
Liver And Kidney ◽  
Haematological Indices

Abstract Considering that the involvement of reactive oxygen species (ROS) has been implicated in the toxicity of various pesticides, this study was designed to study the ameliorative effect of Vitamin E (100 mg/kg body weight) on mice (25 - 30 mg) treated with diazinon (32.5 or 16.25 mg/kg body weight) organophosphate insecticide for 14 days. Subchronic DZN exposure and the protective effects of vitamins E (vitE) were evaluated for their effects on haematological indices, the enzymes concerning liver damage [plasma alanine aminotransferase (ALT), aspartate aminotaransferase (AST), alkaline phosphatise (AIP), and some parameters of kidney function (urea and creatinine) in mice. Additionally, the histopathological changes in liver and kidney tissue were examined. The high dose of diazinon (DZNH) decreased the body weight significantly at the end of experiment. Additionally, the liver and kidney were examines for histopathological changes. The high dose of diazinon decreased body weight significantly. Moreover, there was a statistically significant decrease in haemoglobin (Hb), red blood cell (RBC) and hematocrit (Hct) in diazinon-treated mice compared to controls. This decrease was partially remedied in the diazinon-treated group that also received vitE. Damage in the liver and kidney tissues was also evident as elevated plasma ALT, AST, ALP, urea and creatinine. VitE partially counteracts the toxic effect of DZN and repairs tissue damage in the liver and kidney, especially when supplemented to 1/4 LD50 intoxicated animals. Histopathological changes in liver and kidney were observed only in 32.5 mg/kg DZN given group. These results suggest that the effects of DZN are dose dependent. No pathological findings were observed in vitE + DZN treated groups. According to the present study, we conclude that vitE can reduce the detrimental impacts of diazinon on haematological indicies, as well as liver and kidney function.

scholarly journals Naringenin ameliorates pathological changes in liver and kidney of diabetic mice: a preliminary study / Naringenin reducira histopatološke promjene u jetri i bubregu miševa s dijabetesom

2016 ◽  
Vol 67 (1) ◽  
pp. 19-24 ◽  
Author(s):  
Damir Sirovina ◽  
Nada Oršolić ◽  
Gordana Gregorović ◽  
Marijana Zovko Končić
Keyword(s):  
Lipid Peroxidation ◽  
Kidney Tissue ◽  
Thiobarbituric Acid ◽  
Diabetic Mice ◽  
Histopathological Changes ◽  
Thiobarbituric Acid Test ◽  
Liver And Kidney ◽  
Treatment Of Diabetes ◽  
Preliminary Study ◽  
Acid Test

Abstract The effect of naringenin, a flavonoid found in grapefruit, orange, and tomato, on lipid peroxidation and histopathological changes in the liver and kidneys of alloxan-induced diabetic mice were investigated. Two days after alloxan injection (75 mg kg−1, i.v.), naringenin ethanolic solution (0.5 % v/v) was given to mice intraperitoneally (50 mg kg−1 per day) for seven days. Naringenin’s impact on lipid peroxidation was measured by the 2-thiobarbituric acid test and histopathological changes were examined under a light microscope. Naringenin administration resulted in a significant decrease of lipid peroxidation level in liver and kidney tissue, as well as in a decreased number of vacuolated liver cells and degree of vacuolisation. Indications of tissue repair in kidney suggested that amelioration of diabetes-induced renal damage could be achieved over a longer period of time. Findings suggest that naringenin could be considered a dietary supplement in the prevention or treatment of diabetic complications and other diseases connected with oxidative stress, and gives a hope that it could show similar effects in the treatment of diabetes in humans.

scholarly journals The (-)-epigallocatechin gallate (EGCG) ameliorates hyperglycemia and hyperlipidemia - mediated oxidative stress in liver and kidney of alloxan induced diabetic mice

2020 ◽  
Author(s):  
Ahlem SOUSSI ◽  
Manel GARGOURI ◽  
Sirine AOUEDI ◽  
Amel AKROUTI ◽  
Christian MAGNE ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Fasting Blood Glucose ◽  
Epigallocatechin Gallate ◽  
Future Application ◽  
Antioxidant Enzyme Activities ◽  
Diabetic Mice ◽  
Liver And Kidney ◽  
Health Complications ◽  
Kidney Functions ◽  
Toxicity Indices

Abstract Background: Diabetes mellitus has become a serious problem associated with health complications, such as metabolism disorders and liver-kidney dysfunctions. The inadequacies associated with conventional medicines have led to a determined search for alternative natural therapeutic agents. The present study is conducted to evaluate the hypoglycemic, antilipidemic and antioxidant effects of EGCG in surviving diabetic mice.Methods: Alloxan diabetic mice were injected with EGCG. Their bloods were collected and submitted to various biochemical measurements, including blood glucose, cholesterol, triglycerides, urea, creatinine and transaminases. Their livers and kidneys were isolated to perform histological analysis.Results: Both EGCG and insulin treatments of diabetic mice resulted in a significant reduction in fasting blood glucose level. EGCG supplementation also ameliorated hepatic as well as renal toxicity indices. Moreover, diabetic mice injected by EGCG exhibited significant changes in antioxidant enzyme activities in the liver and kidney. Histological analyses also showed that it exerted an ameliorative action on these organs and efficiently protected the liver-kidney functions of diabetic mice.Conclusion: The findings revealed that EGCG administration induced attractive curative effects on diabetic mice, particularly in terms of liver-kidney functions. EGCG can, therefore, be considered as a potential strong candidate for future application to treat and prevent diabetes.

scholarly journals Minocycline Derived Silver Nanoparticles for Assessment of Their Antidiabetic Potential against Alloxan induced Diabetic Mice

2021 ◽  
Author(s):  
Syed Akif Raza Kazmi ◽  
Muhammad Zahid Qureshi ◽  
Sadia . ◽  
Saleh S Alhewairini ◽  
Shaukat Ali ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Antioxidant Activities ◽  
Islet Cells ◽  
Histopathological Examination ◽  
Radical Scavenging Activity ◽  
Fasting Blood Glucose ◽  
Radical Scavenging ◽  
The Body ◽  
Central Vein ◽  
Diabetic Mice

Diabetes is a life-threatening disease and chronic diabetes affects the parts of the body including the liver, kidney and pancreas. The root cause of diabetes is mainly associated with oxidative stress produced by reactive oxygen species. The minocycline is a polyphenolic drug with excellent antioxidant activities. The objective of the present study was to investigate the antidiabetic potential of minocycline modified silver nanoparticles (Mino/AgNPs) against alloxan-induced diabetic mice. The Mino/AgNPs were synthesized using minocycline as reducing and stabilizing agents. UV-vis, FTIR, X-ray diffraction (XRD) and transmission electron microscopy (TEM) were applied for the characterization of Mino/AgNPs. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay was conducted to determine the antioxidant potential of newly synthesized Mino/AgNPs. The results revealed that the Mino/AgNPs showed higher radical scavenging activity (IC50 = 19.7 &micro;g/mL) as compared to the minocycline (IC50 = 26.0 &micro;g/mL) and ascorbic acid (IC50 = 25.2 &micro;g/mL). Further, the Mino/AgNPs were successfully employed to examine their antidiabetic potential against Alloxan-induced diabetic mice. Hematological results showed that the mice treated with Mino/AgNPs demonstrated a significant decrease in fasting blood glucose level and lipid profile as compared to the diabetic group. The histopathological examination confirmed that the diabetic mice treated with Mino/AgNPs showed significant recovery and revival of histo-morphology of kidney, central vein of liver and islet cells of the pancreas compared to the diabetic mice. Hence Mino/AgNPs have good antidiabetic potential and could be an appropriate nanomedicine to prevent the development of diabetes.

scholarly journals Biochemical and histopathological assessment of atorvastatin-loaded nanoemulsion effectiveness in Rats

2018 ◽  
Vol 7 (2) ◽  
pp. 221
Author(s):  
Khadijah S. Balamash ◽  
Mona S. Al-ddyni
Keyword(s):  
Side Effects ◽  
Lipid Profile ◽  
Histopathological Examination ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Average Diameter ◽  
Serum Glucose ◽  
Lipid Lowering ◽  
Stress Markers ◽  
Liver And Kidney

Background: Atorvastatin (ATV), a lipid lowering agent, has low solubility and poor dissolution affects its oral bioavailability. Nanoemulsion (NE) has been developed to improve the delivery of therapeutic agents. This study was aimed to assess the ability of the NE in enhancing ATV bioavailability and minimizing its side effects in hyperlipidemic rats.Methods: Thirty-five rats divided into seven groups were utilized in this study. Hyperlipidemia was induced by feeding rats high fat diet (HFD) for 3 months. The antihyperlipidemic activity of 10 and 20 mg/kg of ATV loaded in two different delivery systems; nanoemulsion (10% and 20% ATV-LNE) or in water (10% and 20% ATV-sol), were investigated. At the end of the experiment, body weight, serum and plasma biochemical parameters (lipid profile, glucose, insulin, liver and kidney functions, oxidative stress markers were assessed. Liver and kidney were histopathologically examined. The physical characteristics of NE were determined by the Zetasizer (the z-average diameter and zeta potential).Results: 20% ATV-LNE had the smallest nanoparticles (38.12±6.71nm) whereas it had the largest zeta negative potential of -26.8±4.16mV. The serum biochemical results and the histopathological examination revealed that treatment with 20% ATV-LNE improved the lipid profile by significantly increasing HDL and decreasing cholesterol and low-density lipoprotein. Both 10 and 20% ATV-LNE reduced serum glucose level compared to other used formulas.Conclusions: NE formulas have the potential to improve the bioavailability and efficacy of ATV and reduce its side effects.

scholarly journals AQUEOUS EXTRACT OF RED TEA PLANT (Hibiscus sabdariffa L) EFFECT ON THE LIPID PROFILE AND GLUGOSE IN MALE WHITE RABBITS EXPOSED TO OXIDATIVE STRESS

2016 ◽  
Vol 47 (1) ◽  
Author(s):  
S. O. Al-khateeb
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Blood Serum ◽  
Lipid Profile ◽  
Aqueous Extract ◽  
High Density ◽  
The Body ◽  
High Density Lipoproteins ◽  
Animal Blood ◽  
Atherogenic Indices

This study aimed to investigate the of effect aqueous extract of red tea on average of glucose and lipid profile in New Zealand white male rabbits exposed to oxidative stress induced hydrogen peroxide H2O2 of drinking water for the duration 30-days. Treatment of  hydrogen peroxide 2% led alone to a significant increase (P≤0.05) in the concentration of glucose level and the concentration of total cholesterol rate and level of triglycerides and a significant decrease (P ≤ 0.05) in the level of estimating the concentration of high-density cholesterol in the blood serum lipoproteins and a significant increase in the concentration of lipoproteins and very low-lying density cholesterol was significantly higher in atherogenic indices the first and second, respectively, in the blood of animals treated with hydrogen peroxide compared to the control group. It got a significant decrease (P≤0.05) in the concentration of glucose and cholesterol rate and triglycerides and in the concentration of lipoproteins and sessile very density cholesterol and in the atherogenic indices first and second respectively, as happened morally rise in the level of estimating the concentration of high-density lipoproteins cholesterol in the labor animal blood serum aqueous extract of red tea (125 mg / kg of body weight) added all of the hydrogen peroxide compared with the group treated rabbits hydrogen peroxide alone. It is concluded from this current study, the important role of the Red tea as an antioxidant through curb the harmful effects of some types of free radicals within the body and thus improve the most changes biochemical changes in the male albino rabbits  after exposure to oxidative stress Induced by hydrogen Peroxide 2% .

scholarly journals Rejuvenating of Kidney Tissues on Alloxan Induced Diabetic Mice under the Effect of Momordica charantia

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Bhaskar Sharma ◽  
Mohd. Sufiyan Siddiqui ◽  
Gurudayal Ram ◽  
Ranjeet Kumar Yadav ◽  
Arti Kumari ◽  
...  
Keyword(s):  
Body Weight ◽  
Kidney Tissue ◽  
Serum Glucose ◽  
Momordica Charantia ◽  
Histopathological Study ◽  
Diabetic Mice ◽  
Herbal Products ◽  
Mortality And Morbidity ◽  
Chronic Disorder ◽  
Treatment Of Diabetes

Diabetes mellitus is a chronic disorder in human and responsible for different complications and also causes mortality and morbidity. A wide number of herbal products are employed in the treatment of diabetes for their better efficacy and safety compared to synthetic medicine. The present studies have established the antidiabetic potential and rejuvenating capacity of kidney tissues under the effect of extract. Diabetes was induced in the Swiss albino mice by injecting alloxan at the dose of 150 mg/kg body weight and aqueous extract of Momordica charantia fruits at doses of 100 mg/kg body weight and 250 mg/kg body weight was administered orally for three weeks. After 21 days of treatment of the aqueous extracts of Momordica charantia significantly reduces serum glucose level, kidney function tests, lipid peroxidation as well as histopathological study also did show adverse alternation in the morphological architecture of the kidney tissue. Thus, from this study we concluded that Momordica charantia exhibited significant antihyperglycemic and rejuvenating capacity of kidney tissues activities in alloxan induced diabetic mice.

scholarly journals Effect of 5-HTP and L-Dopa on lipid profile of Streptozotocin induced diabetic mice

2014 ◽  
Vol 15 (3) ◽  
pp. 149-155
Author(s):  
Ulka Yadav ◽  
Rajkumari Batham
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Lipid Profile ◽  
Total Cholesterol ◽  
Serum Protein ◽  
Body Weight Gain ◽  
Glycosylated Hemoglobin ◽  
Hdl Cholesterol ◽  
The Body ◽  
Diabetic Mice

The present study was designed to test whether the precursor of serotonin (i.e.5-HTP) and dopamine (i.e. L-DOPA) can influence Body weight and certain hematological constitutes (i.e. blood glucose, glycosylated hemoglobin and serum protein) in streptozotocin induced diabetic mice. In the study, animals were divided into 6 groups. After 10 days at the termination of experiment body weight and blood glucose taken. Blood was collected from left ventricle and deposited to pathology for haematological analysis (i.e. blood Glucose, glycosylated heamoglobin, serum protein, triglyceride total cholesterol, HDL-cholesterol, LDC-cholesterol, and VLDL-cholesterol). Statistical analysis were done with the help of student’s ‘t’ test followed by analysis of variance (ANOVA). The result of the present study suggest that peripheral administration of 5-HTP (a precursor of serotonin) and L-DOPA (a precursor of dopamine) influences the body weight gain and lipid profile (i.e. triglyceride, total cholesterol, HDL-cholesterol , LDL-Cholesterol, VLDC-cholesterol) in streptozotocin induce diabetic mice. The finding of the present study supports the concept that insulin alone does not fully control diabetes. Other chemical substance like neurotransmitter/hormones may involve in controlling the diabetes.

scholarly journals Sleep Quality and Metabolic Disturbance in Public School Teachers of a Brazilian Capital

2020 ◽  
Vol 8 (10) ◽  
pp. 504-517
Author(s):  
Anne Caroline Silva e Silva ◽  
Alice De Sá Ferreira ◽  
Carlos Alberto Alves Dias Filho ◽  
Andressa Coelho Ferreira ◽  
Cristiano Teixeira Mostarda ◽  
...  
Keyword(s):  
Public School ◽  
Sleep Quality ◽  
Lipid Profile ◽  
Fasting Blood Glucose ◽  
Serum Glucose ◽  
Northeastern Brazil ◽  
Poor Sleep ◽  
Public School Teachers ◽  
School Teachers ◽  
Blood Samples

Introduction: Due to high demand and different work schedules, teachers can develop metabolic and sleep disorders. Objective: The aim of this study, therefore, was to verify the quality of sleep and the metabolic changes of public school teachers during an academic semester. Methodology: Longitudinal study, data collection performed during an academic semester, in a public high school in a capital of northeastern Brazil ( lifestyle and disease history, sleep quality - Pittsburgh Sleep Quality Index, blood pressure and anthropometric measurements). Blood samples were collected to determine serum glucose and the lipid profile. All variables were collected at the beginning (Step 1) and at the end (Step 2) of the academic semester with the same teachers. Data analysis was performed using SPSS software version 25.0. Results: The sample consisted of 27 teachers with an average age of 44,7 years. The concentration of fasting blood glucose (92,30 vs 108.85) and total cholesterol (161.00 vs 173.59), were significant (p<0.025 and p<0.001, respectively) when comparing the blood samples at the beginning and end of the school semester. The poor sleep quality and daytime dysfunction showed changes between the values found between the stages of the study, resulting in significant values (p<0.001). Sleep efficiency percentage showed an improvement in the second stage (p<0.004). Conclusion: Through an analysis of the results, it was found that the lipid profile, serum glucose and some sleep variables changed during the academic semester, thus showing the need for care with this category of workers.

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