scholarly journals Application of Co-Culture Technology to Enhance Protease Production by Two Halophilic Bacteria, Marinirhabdus sp. and Marinobacter hydrocarbonoclasticus

Molecules ◽  
2021 ◽  
Vol 26 (11) ◽  
pp. 3141
Author(s):  
Hoang Thi Hong Anh ◽  
Esmaeil Shahsavari ◽  
Nathan J. Bott ◽  
Andrew S. Ball
Keyword(s):  
Wastewater Treatment ◽  
Environmental Conditions ◽  
Protease Activity ◽  
Halophilic Bacteria ◽  
Total Activity ◽  
Protease Production ◽  
Microbial Cultures ◽  
Microbial Strains ◽  
Marinobacter Hydrocarbonoclasticus ◽  
Culture Technology

Although axenic microbial cultures form the basis of many large successful industrial biotechnologies, the production of single commercial microbial strains for use in large environmental biotechnologies such as wastewater treatment has proved less successful. This study aimed to evaluate the potential of the co-culture of two halophilic bacteria, Marinirhabdus sp. and Marinobacter hydrocarbonoclasticus for enhanced protease activity. The co-culture was significantly more productive than monoculture (1.6–2.0 times more growth), with Marinobacter hydrocarbonoclasticus being predominant (64%). In terms of protease activity, enhanced total activity (1.8–2.4 times) was observed in the co-culture. Importantly, protease activity in the co-culture was found to remain active over a much broader range of environmental conditions (temperature 25 °C to 60 °C, pH 4–12, and 10–30% salinity, respectively). This study confirms that the co-culturing of halophilic bacteria represents an economical approach as it resulted in both increased biomass and protease production, the latter which showed activity over arange of environmental conditions.

scholarly journals Microbial Flocculants as an Alternative to Synthetic Polymers for Wastewater Treatment: A Review

Symmetry ◽  
2018 ◽  
Vol 10 (11) ◽  
pp. 556 ◽  
Author(s):  
Faouzi Ben Rebah ◽  
Wissem Mnif ◽  
Saifeldin M. Siddeeg
Keyword(s):  
Heavy Metals ◽  
Wastewater Treatment ◽  
Suspended Solids ◽  
Specific Resistance ◽  
Oxygen Demand ◽  
Synthetic Polymers ◽  
Operating Parameters ◽  
Flocculating Activity ◽  
Microbial Strains ◽  
Active Substances

Microorganisms such as bacteria, fungi, and microalgae have been used to produce bioflocculants with various structures. These polymers are active substances that are biodegradable, environmentally harmless, and have flocculation characteristics. Most of the developed microbial bioflocculants displayed significant flocculating activity (FA > 70–90%) depending on the strain used and on the operating parameters. These biopolymers have been investigated and successfully used for wastewater depollution in the laboratory. In various cases, selected efficient microbial flocculants could reduce significantly suspended solids (SS), turbidity, chemical oxygen demand (COD), total nitrogen (Nt), dye, and heavy metals, with removal percentages exceeding 90% depending on the bioflocculating materials and on the wastewater characteristics. Moreover, bioflocculants showed acceptable results for sludge conditioning (accepted levels of dry solids, specific resistance to filtration, moisture, etc.) compared to chemicals. This paper explores various bioflocculants produced by numerous microbial strains. Their production procedures and flocculating performance will be included. Furthermore, their efficiency in the depollution of wastewater will be discussed.

scholarly journals Evaluation of bacterial proliferation with a microfluidic-based device: Antibiochip

2019 ◽  
Author(s):  
Valentina Gallo ◽  
Alessia Ruiba ◽  
Massimo Zanin ◽  
Paolo Begnamino ◽  
Sabina Ledda ◽  
...  
Keyword(s):  
Laboratory Medicine ◽  
Culture Conditions ◽  
Inhibition Of Proliferation ◽  
Standard Methods ◽  
E Coli ◽  
Bacterial Proliferation ◽  
Microbial Cultures ◽  
Novel Approach ◽  
Microbial Strains ◽  
The Relationship

AbstractThe measurement of the proliferation (and the relevant inhibition of proliferation) of microbes is used in different settings, from industry to laboratory medicine. Thus, in this study, the capacity of the Antibiochip (ELTEK spa), a microfluidic-based device, to measure the amount of E. coli in certain culture conditions, was evaluated. An Antibiochip is composed of V-shaped microchannels, and the amount of microparticles (such as microbes) is measured by the surface of the pellet after centrifugation. In the present study, different geometries, volumes and times were analyzed. When the best conditions were identified, serial dilutions of microbial cultures were tested to validate the linearity of the results. Then, with the use of wild E. coli strains isolated from medical samples, the relationship between bacterial susceptibility to antibiotics (gentamicin, amikacin and ceftriaxone) measured by standard methods and that measured by the Antibiochip was evaluated. In this report, the good quality performances of the methods, their linearity and the capacity to identify susceptible microbial strains after 60 minutes of incubation are shown. These results represent a novel approach for ultrarapid antibiograms in clinics.

High performance GO/MXene/PPS composite filtration membrane for dye wastewater treatment under harsh environmental conditions

2022 ◽  
Vol 29 ◽  
pp. 101017
Author(s):  
Xin-Yu Ma ◽  
Ting-Ting Fan ◽  
Gang Wang ◽  
Zhen-Huan Li ◽  
Jia-Horng Lin ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Environmental Conditions ◽  
High Performance ◽  
Dye Wastewater ◽  
Filtration Membrane

Production and characterisation of thermostable alkaline protease from Bacillus subtilis isolated from LA hot spring, Terengganu

2021 ◽  
Vol 16 (7) ◽  
pp. 84-91
Author(s):  
Maslinda Alias ◽  
Hakim Che Harun Mohammad ◽  
Ashraf Razali Nurul ◽  
Jasnizat Saidin ◽  
Nazaitulshila Rasit ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Alkaline Protease ◽  
Protease Activity ◽  
Hot Spring ◽  
Skim Milk ◽  
Industrial Applications ◽  
Protease Production ◽  
Significant Activity ◽  
Original Activity ◽  
Optimum Ph

This research aims to produce thermostable alkaline protease from Bacillus subtilis isolated from La Hot Spring, Terengganu, Malaysia. The study was also conducted to determine the optimum conditions for protease production and stability by considering several parameters including pH, temperature and salt concentration. All seven bacteria were screened on skim milk agar overnight at 37 °C. Three strains with the highest proteolytic activity were identified in protease specific medium. The thermostable alkaline protease had an optimum temperature of 60 °C which achieved 85.73, 82.90 and 83.05 U/mL of protease activity for the three strains respectively. Furthermore, the strains exhibited significant activity of more than 90% from their original activity. Meanwhile, the optimum pH for protease production was pH 9 with the protease activity of 76.76, 79.71 and 88.39 U/mL for TB4, TB6 and TB9 strains, respectively. Proteases were found stable at pH 9 where the loss did not exceed 30% of its original activity. Collectively, all of the data emphasised that proteases from B. subtilis were alkaline thermostable proteases in accordance with a recent report. The finding highlights the viability of the proteases for biotechnological and industrial applications.

Purification and characterization of exocellular proteases produced by a clinical isolate and a laboratory strain of Pseudomonas aeruginosa

1980 ◽  
Vol 26 (1) ◽  
pp. 77-86 ◽  
Author(s):  
S. E. Jensen ◽  
L. Phillippe ◽  
J. Teng Tseng ◽  
G. W. Stemke ◽  
J. N. Campbell
Keyword(s):  
Molecular Weight ◽  
Pseudomonas Aeruginosa ◽  
Clinical Isolate ◽  
Protease Activity ◽  
Gel Filtration ◽  
Laboratory Strain ◽  
Exchange Chromatography ◽  
The Other ◽  
Protease Production ◽  
Peptide Bonds

Exocellular protease production was examined in two separate strains of Pseudomonas aeruginosa, one a clinical isolate and the other a laboratory strain. Both strains produced two separate proteases (proteases 1 and 2) which were indistinguishable from one strain to the other. The two proteases were purified by a two-step procedure of gel filtration chromatography followed by ion-exchange chromatography. Proteases 1 and 2 were shown to be distinct serologically and unrelated by physicochemical parameters examined. Protease 1 was the major exocellular protein produced and contributed about 95% of the total protease activity of the culture. It was estimated to have a molecular weight of 34 850 and was also shown to contain 10% glucosamine by weight. Protease 2, in contrast, had an estimated molecular weight of 52750 and contained no detectable carbohydrate. Proteases 1 and 2 were both stimulated by Ca2+, and Mg2+ and inhibited by Co2+Zn2+, and 1,10-o-phenanthroline. Protease 1 was also inhibited by EDTA. In addition to protease activity, both proteases 1 and 2 demonstrated elastase activity as well as a limited collagenase activity. Specificity of the two proteases against synthetic peptides was, however, quite different. Protease 1, but not protease 2, showed a preference for peptide bonds in which the amino group was contributed by an amino acid with a hydrophobic R group.

Optimized Environmental Conditions for Petroleum Degradation of Strain B1 by Orthogonal Test

2011 ◽  
Vol 393-395 ◽  
pp. 1308-1312
Author(s):  
Chun Xu Wu ◽  
Hong Tao Zhang ◽  
Bei Hai Zhou ◽  
Lei Gao ◽  
Hao Deng ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Wastewater Treatment ◽  
Environmental Impact ◽  
Environmental Conditions ◽  
Treatment Plant ◽  
Orthogonal Test ◽  
Oil Field ◽  
Impact Factors ◽  
Factors Influencing ◽  
Biodegradation Efficiency

A strain B1 belonging to Pseudomonas aeruginosa with high petroleum-degrading efficiency was isolated from Dagang oil-field wastewater treatment plant. More than 80% petroleum-degrading efficiency was obtained at 32°C, 200 r/min. According to the orthogonal test, the environmental impact factors influencing petroleum-degrading efficiency of the strain were investigated. The optimum environmental conditions for petroleum degradation were as follows: temperature was 40 °C, pH 8.0, oil inoculation 1 mL, nitrogen content 0.05 g. And the biodegradation efficiency of the strain reached 86.1%.

Extracellular Heat-Resistant Proteases of Psychrotrophic Pseudomonads

1983 ◽  
Vol 46 (2) ◽  
pp. 90-94 ◽  
Author(s):  
THAKOR R. PATEL ◽  
FRANCIS M. BARTLETT ◽  
JAWED HAMID
Keyword(s):  
Protease Activity ◽  
Milk Powder ◽  
Milk Proteins ◽  
Raw Milk ◽  
Enzyme Synthesis ◽  
Maximum Activity ◽  
Protease Production ◽  
Divalent Metal Ions ◽  
Extracellular Proteases ◽  
Heat Resistant

Several bacterial isolates from raw milk produced proteases. Most of such 28 isolates were gram-negative rods which were oxidase- and catalase-positive. All the isolates grew at temperatures in the range of 0–35°C, but failed to grow at 37°C. Nineteen of these isolates were tentatively assigned to genus Pseudomonas, and were used in the present investigation. Extracellular proteases from these psychrotrophic pseudomonads were heat-resistant, being able to retain partial activity even after heat-treatment at 120°C for 10 min. Milk proteins were preferred substrates by these proteases although some also hydrolysed bovine serum albumin, hemoglobin and ovalbumin. The optimum pH for the maximum activity was between pH 7.2 and 7.4. Divalent metal ions like Cu2+, Co2+, Hg2+, and Zn2+ were inhibitory to protease activity while Ca2+, Mg2+, and Mn2+ had little or no inhibitory effect on the proteases. Induced levels of protease production were observed when cultures were grown in minimal media containing either casein or nonfat dried milk powder. Glucose, citrate and lactose repressed enzyme synthesis in a minmal salts medium containing either casein or nonfat dried milk powder. Protease activity was also detected in cultures grown in minimal medium containing glutamine. Proteases from different isolates varied in their molecular weights.

Photodegradation of emerging contaminants in a sunlit wastewater lagoon, seasonal measurements, environmental impacts and modelling

2020 ◽  
Vol 6 (12) ◽  
pp. 3380-3390
Author(s):  
Yufei Wang ◽  
Linhua Fan ◽  
Nick Crosbie ◽  
Felicity A. Roddick
Keyword(s):  
Wastewater Treatment ◽  
Environmental Impacts ◽  
Environmental Conditions ◽  
Emerging Contaminants ◽  
Apex Model

Relationships between seasonal environmental conditions and photodegradation of emerging contaminants during lagoon wastewater treatment demonstrated and APEX model evaluated.

Highly active and stable protease production by an extreme halophilic archaeon Haloarcula sp. TG1 isolated from Lake Tuz, Turkey

2017 ◽  
Vol 42 (3) ◽  
Author(s):  
Büşra Abanoz ◽  
Sezer Okay ◽  
Aslıhan Kurt-Kızıldoğan
Keyword(s):  
Protease Activity ◽  
Protease Production ◽  
Extreme Conditions ◽  
Salt Mine ◽  
Producer Strain ◽  
Extreme Halophiles ◽  
Highly Active ◽  
Protease Enzyme ◽  
Wide Range

AbstractObjective:Isolation of halophilic microorganisms from Çankırı salt mine and Lake Tuz in Turkey to explore versatile protease producers for industry and characterization of protease enzyme from the best protease producer among the isolated strains.Methods:Extreme halophiles were isolated from salt samples of Çankırı salt mine and Lake Tuz. Their protease activities were determined. The isolate with the highest protease activity was characterized. Its protease activity was evaluated in different NaCl concentrations, temperature and pH ranges, and in the presence of different inhibitors and metals. Thermostability and pH stability were also determined.Results:The highest protease producer strain was identified asConclusion:Due to the high activity and stability over a wide range of extreme conditions,

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