scholarly journals Metabolomics Analyses of Cotyledon and Plumule Showing the Potential Domestic Selection in Lotus Breeding

Molecules ◽  
2021 ◽  
Vol 26 (4) ◽  
pp. 913
Author(s):  
Huanhuan Qi ◽  
Feng Yu ◽  
Rebecca Njeri Damaris ◽  
Pingfang Yang
Keyword(s):  
Amino Acids ◽  
Functional Food ◽  
Ultra Performance Liquid Chromatography ◽  
Chemical Components ◽  
Tandem Mass ◽  
Targeted Metabolomics ◽  
Amino Acids And Derivatives ◽  
Widely Targeted Metabolomics ◽  
Wild Variety ◽  
Breeding Selection

Lotus (Nelumbo nucifera) seeds are widely consumed as functional food or herbal medicine, of which cotyledon (CL) is the main edible part, and lotus plumule (LP) is commonly utilized in traditional Chinese medicine. However, few studies have been conducted to investigate the chemical components of CL and LP in dry lotus seeds, not to mention the comparison between wild and domesticated varieties. In this study, a widely targeted metabolomics approach based on Ultra Performance Liquid Chromatography-electrospray ionization-Tandem mass spectrometry (UPLC-ESI-MS/MS) was utilized to analyze the metabolites in CL and LP of China Antique (“CA”, a wild variety) and Jianxuan-17 (“JX”, a popular cultivar). A total of 402 metabolites were identified, which included flavonoids (23.08% to 27.84%), amino acids and derivatives (14.18–16.57%), phenolic acids (11.49–12.63%), and lipids (9.14–10.95%). These metabolites were classified into ten clusters based on their organ or cultivar-specific characters. Most of these metabolites were more abundant in LP than in CL for both varieties, except for metabolites belonging to organic acids and lipids. The analysis of differentially accumulated metabolites (DAMs) demonstrated that more than 25% of metabolites detected in our study were DAMs in CL and LP comparing “JX” with “CA”, most of which were less abundant in “JX”, including 35 flavonoids in LP, 23 amino acids and derivatives in CL, 7 alkaloids in CL, and 10 nucleotides and derivatives in LP, whereas all of 11 differentially accumulated lipids in LP were more abundant in “JX”. Together with the fact that the seed yield of “JX” is much higher than that of “CA”, these results indicated that abundant metabolites, especially the functional secondary metabolites (mainly flavonoids and alkaloids), were lost during the process of breeding selection.

scholarly journals Profiling of Widely Targeted Metabolomics for the Identification of Secondary Metabolites in Heartwood and Sapwood of the Red-Heart Chinese Fir (Cunninghamia Lanceolata)

Forests ◽  
2020 ◽  
Vol 11 (8) ◽  
pp. 897
Author(s):  
Sen Cao ◽  
Zijie Zhang ◽  
Yuhan Sun ◽  
Yun Li ◽  
Huiquan Zheng
Keyword(s):  
Secondary Metabolites ◽  
Ultra Performance Liquid Chromatography ◽  
Chinese Fir ◽  
Wood Products ◽  
Cunninghamia Lanceolata ◽  
Flavonoid Pathway ◽  
Targeted Metabolomics ◽  
System A ◽  
Widely Targeted Metabolomics ◽  
Mass Spectrometry System

The chemical composition of secondary metabolites is important for the quality control of wood products. In this study, the widely targeted metabolomics approach was used to analyze the metabolic profiles of heartwood and sapwood in the red-heart Chinese fir (Cunninghamia lanceolata), with an ultra-performance liquid chromatography-electrospray ionization tandem mass spectrometry system. A total of 224 secondary metabolites were detected in the heartwood and sapwood, and of these, flavonoids and phenolic acids accounted for 36% and 26% of the components, respectively. The main pathways appeared to be differentially activated, including those for the biosynthesis of phenylpropanoids and flavonoids. Moreover, we observed highly significant accumulation of naringenin chalcone, dihydrokaempferol, pinocembrin, hesperetin, and other important secondary metabolites in the flavonoid biosynthesis pathway. Our results provide insight into the flavonoid pathway associated with wood color formation in Chinese fir that will be useful for further breeding programs.

scholarly journals A rapid ultra performance liquid chromatography tandem mass spectrometric method for measuring amino acids associated with maple syrup urine disease, tyrosinaemia and phenylketonuria

2007 ◽  
Vol 44 (5) ◽  
pp. 474-481 ◽  
Author(s):  
Scott Freeto ◽  
Donald Mason ◽  
Jie Chen ◽  
Robert H Scott ◽  
Srinivas B Narayan ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Amino Acids ◽  
Amino Acid ◽  
Tandem Mass Spectrometry ◽  
Maple Syrup Urine Disease ◽  
Ultra Performance Liquid Chromatography ◽  
Exchange Chromatography ◽  
Tandem Mass ◽  
Maple Syrup ◽  
Urine Disease

Background: Patients with inherited disorders of amino acid metabolism including maple syrup urine disease, tyrosinaemia and phenylketonuria on dietary management require frequent monitoring of disease-relevant plasma amino acids in order to optimize therapeutic benefit. Poorly controlled maple syrup urine disease in particular may result in catastrophic metabolic decompensation. Most methods for monitoring amino acid concentrations are time-consuming and have clinically impractical turnaround times, particularly when the required time to run standards and control samples is taken into account. Methods: We have analysed plasma amino acids using standard ion-exchange chromatography with ninhydrin detection in an amino acid analyser and compared the data with that obtained for the same samples using ultra-performance liquid chromatography (UPLCTM) separation with detection by tandem mass spectrometry. Results: The two methodologies compared very well for the measurement of six important amino acids with correlation coefficients greater than 0.96 for all. The time for sample preparation was longer for the UPLC methodology as batched derivatization and evaporation is required but UPLC-tandem mass spectrometry generated sample results every 8 min while conventional ion-exchange chromatography took almost 1 h per sample. Conclusion: UPLC-tandem mass spectrometry generates data that compares well with existing 'gold standard' methodologies but significantly reduces sample turnaround time. Decreasing the turnaround time for amino acid analyses is very likely to improve clinical care for patients with amino acid disorders as dietary adjustments can be made sooner.

Targeted metabolomics reveals dynamic portrayal of amino acids and derivatives in triple-negative breast cancer cells and culture media

2021 ◽  
Author(s):  
Fang Kou ◽  
Bangjie Zhu ◽  
Wenbin Zhou ◽  
Chunming Lv ◽  
Yu Cheng ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Amino Acids ◽  
Cancer Cells ◽  
Triple Negative Breast Cancer ◽  
Breast Cancer Cells ◽  
Triple Negative ◽  
Culture Media ◽  
Targeted Metabolomics ◽  
Amino Acids And Derivatives

Targeted metabolomics reveals dynamic portrayal of amino acids and derivatives in triple-negative breast cancer cells and culture media.

scholarly journals Comparative analysis of tuberous root metabolites between cultivated and wild varieties of Rehmannia glutinosa by widely targeted metabolomics

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Yanqing Zhou ◽  
Luying Shao ◽  
Jialin Zhu ◽  
Huimin Li ◽  
Hongying Duan
Keyword(s):  
Phenolic Acids ◽  
Rehmannia Glutinosa ◽  
Targeted Metabolomics ◽  
Kegg Pathways ◽  
Tuberous Roots ◽  
Comprehensive Development ◽  
Widely Targeted Metabolomics ◽  
Development And Utilization ◽  
Wild Variety

AbstractDifferential metabolites between tuberous roots from cultivated variety (ZP) and wild variety (YS) of Rehmannia glutinosa were analyzed by widely targeted metabolomics, and annotated to KEGG pathways. 228 secondary metabolites (SM) in ZP and YS were detected, of which 58 were differential metabolites (DM), including 41 flavonoids, 10 phenolic acids, 3 terpenoids, 2 alkaloids and 2 others, and 170 were unchanged; Among 58 DMs, 44 (75.9%) were up-regulated in YS, of which 30 were unique to YS, while 14 (24.1%) were down-regulated in YS, of which 10 were unique to ZP; Among flavonoids, 33 (80.5%) were more highly expressed in YS than in ZP; Among phenolic acids, 7 (70%) were more highly expressed in YS than in ZP; 12 of 58 DMs were annotated into 17 types of KEGG pathways. Among them, benzoic acid and p-Coumaryl alcohol were up-regulated in YS, and annotated into 10 pathways (58.8%) and 4 pathways (23.5%), respectively. In addition, much of DMs possess various pharmacological effects. These results indicated better quality of YS than ZP and the necessity of YS domestication. Taken together, this study will provide a reference for the scientific introduction, comprehensive development and utilization of wild Rehmannia glutinosa.

scholarly journals Comparative Analysis of Amino Acids, Nucleosides, and Nucleobases inThais clavigerafrom Different Distribution Regions by Using Hydrophilic Interaction Ultra-Performance Liquid Chromatography Coupled with Triple Quadrupole Tandem Mass Spectrometry

2015 ◽  
Vol 2015 ◽  
pp. 1-10 ◽  
Author(s):  
Yahui Ge ◽  
Yuping Tang ◽  
Sheng Guo ◽  
Xin Liu ◽  
Zhenhua Zhu ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Amino Acids ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Essential Amino Acids ◽  
Quality Standard ◽  
Ultra Performance Liquid Chromatography ◽  
Tandem Mass ◽  
Hydrophilic Interaction ◽  
Thais Clavigera

Thais clavigera, as function food, is distributed widely along the coasts of China. To compare and tap its potentially nutritional and functional values, hydrophilic interaction ultra-performance liquid chromatography coupled with triplequadrupole tandem mass spectrometry (HILIC-UPLC-TQ-MS/MS) was used for simultaneous identification and quantification of amino acids, nucleosides, and nucleobases in the extracts ofT. clavigerafrom 19 sea areas in China, and a PCA was further performed for comparing their content variation in different distribution regions. The total contents of amino acids varied from 116.74 mg/g to 298.58 mg/g being higher than contents of nucleosides and nucleobases that varied from 2.65 mg/g and 20.49 mg/g. Among the habitats, Hainan province had content advantages on others. By PCA, samples collected from different regions were classified into three groups. For specific constituents, lysine accounted for large part of essential amino acids; glycine and taurine also play important roles in the delicate taste and health care function of it. Inosine takes up most of total contents of nucleosides and nucleobases. These results provided good data for establishing quality standard ofT. clavigerarelated products and their further development and utilization.

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