scholarly journals Profiling of Widely Targeted Metabolomics for the Identification of Secondary Metabolites in Heartwood and Sapwood of the Red-Heart Chinese Fir (Cunninghamia Lanceolata)

Forests ◽  
2020 ◽  
Vol 11 (8) ◽  
pp. 897
Author(s):  
Sen Cao ◽  
Zijie Zhang ◽  
Yuhan Sun ◽  
Yun Li ◽  
Huiquan Zheng
Keyword(s):  
Secondary Metabolites ◽  
Ultra Performance Liquid Chromatography ◽  
Chinese Fir ◽  
Wood Products ◽  
Cunninghamia Lanceolata ◽  
Flavonoid Pathway ◽  
Targeted Metabolomics ◽  
System A ◽  
Widely Targeted Metabolomics ◽  
Mass Spectrometry System

The chemical composition of secondary metabolites is important for the quality control of wood products. In this study, the widely targeted metabolomics approach was used to analyze the metabolic profiles of heartwood and sapwood in the red-heart Chinese fir (Cunninghamia lanceolata), with an ultra-performance liquid chromatography-electrospray ionization tandem mass spectrometry system. A total of 224 secondary metabolites were detected in the heartwood and sapwood, and of these, flavonoids and phenolic acids accounted for 36% and 26% of the components, respectively. The main pathways appeared to be differentially activated, including those for the biosynthesis of phenylpropanoids and flavonoids. Moreover, we observed highly significant accumulation of naringenin chalcone, dihydrokaempferol, pinocembrin, hesperetin, and other important secondary metabolites in the flavonoid biosynthesis pathway. Our results provide insight into the flavonoid pathway associated with wood color formation in Chinese fir that will be useful for further breeding programs.

scholarly journals Widely Targeted Metabolomics Analysis Reveals Great Changes in Nonvolatile Metabolites of Oolong Teas during Long-Term Storage

Molecules ◽  
2021 ◽  
Vol 26 (23) ◽  
pp. 7278
Author(s):  
Cuiyun Hong ◽  
Wenjie Yue ◽  
Qingyu Shen ◽  
Wenhua Wang ◽  
Hongyan Meng ◽  
...  
Keyword(s):  
Chemical Change ◽  
Ultra Performance Liquid Chromatography ◽  
Special Period ◽  
Targeted Metabolomics ◽  
Long Term Storage ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass ◽  
Widely Targeted Metabolomics ◽  
Term Storage

As a semifermented tea, oolong is exceedingly popular worldwide for its elegant, flowery aroma and mellow, rich taste. However, recent marketing trends for old oolong teas and their chemical quality largely remain unexplored. In this study, we applied widely targeted metabolomics using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) combined with multivariate analysis to investigate the chemical change of oolong teas in the aging process. With the increasing of store time, most nongalloylated catechins; tannins, including TFs and proanthocyanidins; flavonols and glycosylated flavonols; amino acids and their derivatives; nucleotides and their derivatives; and lots of alkaloids and phospholipids declined, while most fatty acids and organic acids increased, and galloylated catechins, GA, and caffeine were almost stable. The result also suggested that approximately seven years (but not an infinite extension) was a special period for oolong tea storage, which brings about excellent taste.

scholarly journals Metabolomics Analyses of Cotyledon and Plumule Showing the Potential Domestic Selection in Lotus Breeding

Molecules ◽  
2021 ◽  
Vol 26 (4) ◽  
pp. 913
Author(s):  
Huanhuan Qi ◽  
Feng Yu ◽  
Rebecca Njeri Damaris ◽  
Pingfang Yang
Keyword(s):  
Amino Acids ◽  
Functional Food ◽  
Ultra Performance Liquid Chromatography ◽  
Chemical Components ◽  
Tandem Mass ◽  
Targeted Metabolomics ◽  
Amino Acids And Derivatives ◽  
Widely Targeted Metabolomics ◽  
Wild Variety ◽  
Breeding Selection

Lotus (Nelumbo nucifera) seeds are widely consumed as functional food or herbal medicine, of which cotyledon (CL) is the main edible part, and lotus plumule (LP) is commonly utilized in traditional Chinese medicine. However, few studies have been conducted to investigate the chemical components of CL and LP in dry lotus seeds, not to mention the comparison between wild and domesticated varieties. In this study, a widely targeted metabolomics approach based on Ultra Performance Liquid Chromatography-electrospray ionization-Tandem mass spectrometry (UPLC-ESI-MS/MS) was utilized to analyze the metabolites in CL and LP of China Antique (“CA”, a wild variety) and Jianxuan-17 (“JX”, a popular cultivar). A total of 402 metabolites were identified, which included flavonoids (23.08% to 27.84%), amino acids and derivatives (14.18–16.57%), phenolic acids (11.49–12.63%), and lipids (9.14–10.95%). These metabolites were classified into ten clusters based on their organ or cultivar-specific characters. Most of these metabolites were more abundant in LP than in CL for both varieties, except for metabolites belonging to organic acids and lipids. The analysis of differentially accumulated metabolites (DAMs) demonstrated that more than 25% of metabolites detected in our study were DAMs in CL and LP comparing “JX” with “CA”, most of which were less abundant in “JX”, including 35 flavonoids in LP, 23 amino acids and derivatives in CL, 7 alkaloids in CL, and 10 nucleotides and derivatives in LP, whereas all of 11 differentially accumulated lipids in LP were more abundant in “JX”. Together with the fact that the seed yield of “JX” is much higher than that of “CA”, these results indicated that abundant metabolites, especially the functional secondary metabolites (mainly flavonoids and alkaloids), were lost during the process of breeding selection.

Age-dependent microRNAs in regulation of vascular cambium activity in Chinese fir (Cunninghamia lanceolata)

Trees ◽  
2021 ◽  
Author(s):  
Guijun Liu ◽  
Xian Xue ◽  
Jinling Feng ◽  
Dechang Cao ◽  
Jinxing Lin ◽  
...  
Keyword(s):  
Chinese Fir ◽  
Cunninghamia Lanceolata ◽  
Vascular Cambium ◽  
Age Dependent ◽  
Cambium Activity

scholarly journals Extensive Metabolite Profiling in the Unexploited Organs of Black Tiger for Their Potential Valorization in the Pharmaceutical Industry

Life ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 544
Author(s):  
Jianfei Gao ◽  
Kangning Xiong ◽  
Wei Zhou ◽  
Weijie Li
Keyword(s):  
Acid Metabolism ◽  
Metabolite Profile ◽  
Industrial Applications ◽  
Targeted Metabolomics ◽  
Arginine Biosynthesis ◽  
Nutritional Values ◽  
Phenylpropanoid Biosynthesis ◽  
Tyrosine Metabolism ◽  
Organ Specific ◽  
Widely Targeted Metabolomics

Black tiger (Kadsura coccinea (Lem.)) has been reported to hold enormous pharmaceutical potential. The fruit and rhizome of black tiger are highly exploited in the pharmaceutical and other industries. However, the most important organs from the plant such as the leaf and stem are considered biowastes mainly because a comprehensive metabolite profile has not been reported in these organs. Knowledge of the metabolic landscape of the unexploited black tiger organs could help identify and isolate important compounds with pharmaceutical and nutritional values for a better valorization of the species. In this study, we used a widely targeted metabolomics approach to profile the metabolomes of the K. coccinea leaf (KL) and stem (KS) and compared them with the root (KR). We identified 642, 650 and 619 diverse metabolites in KL, KS and KR, respectively. A total of 555 metabolites were mutually detected among the three organs, indicating that the leaf and stem organs may also hold potential for medicinal, nutritional and industrial applications. Most of the differentially accumulated metabolites between organs were enriched in flavone and flavonol biosynthesis, phenylpropanoid biosynthesis, arginine and proline metabolism, arginine biosynthesis, tyrosine metabolism and 2-oxocarboxylic acid metabolism pathways. In addition, several important organ-specific metabolites were detected in K. coccinea. In conclusion, we provide extensive metabolic information to stimulate black tiger leaf and stem valorization in human healthcare and food.

Effects of UV light on the physic-chemical properties of yerba-mate

2015 ◽  
Vol 45 (2) ◽  
pp. 221-228 ◽  
Author(s):  
Cleomara Salete Lewinski ◽  
Itamar Luís Gonçalves ◽  
Ana Cláudia Piovezan Borges ◽  
Nessana Dartora ◽  
Lauro Mera de Souza ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Uv Light ◽  
Chemical Properties ◽  
Ultra Performance Liquid Chromatography ◽  
Yerba Mate ◽  
Content Type ◽  
Sensory Acceptance ◽  
Array Detectors ◽  
Photodiode Array ◽  
Novel Strategy

Purpose – The aim of this paper is to evaluate the effects of ultraviolet (UV) light on the color, secondary metabolites and sensory acceptance of processed yerba-mate. Design/methodology/approach – Samples were exposed to UV light for 72 hours. The colorimetric coordinates (L*, a* and b*) were analyzed every 6 hours, while secondary metabolites and sensory acceptance were assessed at the beginning and at the end of the experiment. Methylxanthines and phenolic compounds were quantified by ultra performance liquid chromatography photodiode array detectors and vegetable pigments by UV/visible spectrophotometry. Findings – Decreases in methylxanthines, rutin and isomers of chlorogenic acids were found, along with an increase in isomers of dicaffeoylquinic acids. The product showed less sensory acceptance compared to the control. These results show that UV light treatment of yerba-mate accelerates the maturation process. Practical implications – UV light can be used in yerba-mate maturation with a reduction time and can ensure microbiological safety with small changes in its phytochemical profile. Originality/value – This paper is the first report of a novel strategy to investigate the yerba-mate maturation using UV light.

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