scholarly journals Characterization of a Novel Laccase LAC-Yang1 from White-Rot Fungus Pleurotus ostreatus Strain Yang1 with a Strong Ability to Degrade and Detoxify Chlorophenols

Molecules ◽  
2021 ◽  
Vol 26 (2) ◽  
pp. 473
Author(s):  
Xinping Liu ◽  
Wei Deng ◽  
Yang Yang
Keyword(s):  
Ethylene Glycol ◽  
Metal Ions ◽  
Organic Solvents ◽  
Pleurotus Ostreatus ◽  
Metal Ion ◽  
Strong Stability ◽  
White Rot ◽  
White Rot Fungus ◽  
Alkaline Conditions ◽  
High Concentrations

In this study, a laccase LAC-Yang1 was successfully purified from a white-rot fungus strain Pleurotus ostreatus strain yang1 with high laccase activity. The enzymatic properties of LAC-Yang1 and its ability to degrade and detoxify chlorophenols such as 2,6-dichlorophenol and 2,3,6-trichlorophenol were systematically studied. LAC-Yang1 showed a strong tolerance to extremely acidic conditions and strong stability under strong alkaline conditions (pH 9–12). LAC-Yang1 also exhibited a strong tolerance to different inhibitors (EDTA, SDS), metal ions (Mn2+, Cu2+, Mg2+, Na+, K+, Zn2+, Al3+, Co2+, and metal ion mixtures), and organic solvents (glycerol, propylene glycol). LAC-Yang1 showed good stability in the presence of Mg2+, Mn2+, glycerol, and ethylene glycol. Our results reveal the strong degradation ability of this laccase for high concentrations of chlorophenols (especially 2,6-dichlorophenol) and chlorophenol mixtures (2,6-dichlorophenol + 2,3,6-trichlorophenol). LAC-Yang1 displayed a strong tolerance toward a variety of metal ions (Na2+, Zn2+, Mn2+, Mg2+, K+ and metal ion mixtures) and organic solvents (glycerol, ethylene glycol) in its degradation of 2,6-dichlorophenol and 2,3,6-trichlorophenol. The phytotoxicity of 2,6-dichlorophenol treated by LAC-Yang1 was significantly reduced or eliminated. LAC-Yang1 demonstrated a good detoxification effect on 2,6-dichlorophenol while degrading this compound. In conclusion, LAC-Yang1 purified from Pleurotus ostreatus has great application value and potential in environmental biotechnology, especially the efficient degradation and detoxification of chlorophenols.

Efficient Preconcentration of Cu2+, Zn2+ and Fe3+ with an Agarose-Schiff Base Sorbent

2007 ◽  
Vol 72 (7) ◽  
pp. 908-916 ◽  
Author(s):  
Payman Hashemi ◽  
Hatam Hassanvand ◽  
Hossain Naeimi
Keyword(s):  
Schiff Base ◽  
Metal Ions ◽  
Metal Ion ◽  
Good Accuracy ◽  
Kinetic Studies ◽  
Sample Volume ◽  
Effects Of Ph ◽  
Glass Column ◽  
High Concentrations ◽  
Ph Range

Sorption and preconcentration of Cu2+, Zn2+ and Fe3+ on a salen-type Schiff base, 2,2'- [ethane-1,2-diylbis(nitrilomethylidyne)]bis(2-methylphenol), chemically immobilized on a highly crosslinked agarose support, were studied. Kinetic studies showed higher sorption rates of Cu2+ and Fe3+ in comparison with Zn2+. Half-times (t1/2) of 31, 106 and 58 s were obtained for sorption of Cu2+, Zn2+ and Fe3+ by the sorbent, respectively. Effects of pH, eluent concentration and volume, ionic strength, buffer concentration, sample volume and interferences on the recovery of the metal ions were investigated. A 5-ml portion of 0.4 M HCl solution was sufficient for quantitative elution of the metal ions from 0.5 ml of the sorbent packed in a 6.5 mm i.d. glass column. Quantitative recoveries were obtained in a pH range 5.5-6.5 for all the analytes. The volumes to be concentrated exceeding 500 ml, ionic strengths as high as 0.5 mol l-1, and acetate buffer concentrations up to 0.3 mol l-1 for Zn2+ and 0.4 mol l-1 for Cu2+ and Fe3+ did not have any significant effect on the recoveries. The system tolerated relatively high concentrations of diverse ions. Preconcentration factors up to 100 and detection limits of 0.31, 0.16 and 1.73 μg l-1 were obtained for Cu2+, Zn2+ and Fe3+, respectively, for their determination by a flame AAS instrument. The method was successfully applied to the metal ion determinations in several river water samples with good accuracy.

The screening of culture condition and properties of xylanase by white-rot fungus Pleurotus ostreatus

2004 ◽  
Vol 39 (11) ◽  
pp. 1561-1566 ◽  
Author(s):  
Cai Qinnghe ◽  
Yue Xiaoyu ◽  
Niu Tiangui ◽  
Ji Cheng ◽  
Ma Qiugang
Keyword(s):  
Culture Condition ◽  
Pleurotus Ostreatus ◽  
White Rot ◽  
White Rot Fungus

Lignocellulolytic activity of Pleurotus ostreatus under solid state fermentation using silage, stover, and cobs of maize

BioResources ◽  
2021 ◽  
Vol 16 (2) ◽  
pp. 3797-3807
Author(s):  
Magdah Ganash ◽  
Tarek M. Abdel Ghany ◽  
Mohamed A. Al Abboud ◽  
Mohamed M. Alawlaqi ◽  
Husam Qanash ◽  
...  
Keyword(s):  
Solid State ◽  
Incubation Period ◽  
Solid State Fermentation ◽  
Pleurotus Ostreatus ◽  
White Rot Fungi ◽  
Value Added ◽  
Extracellular Protein ◽  
White Rot ◽  
White Rot Fungus ◽  
Lignocellulosic Substrate

Lignocellulolytic white-rot fungi allow the bioconversion of agricultural wastes into value-added products that are used in a myriad of applications. The aim of this work was to use corn residues (Zea mays L.) to produce valuable products under solid-state fermentation (SSF) with Pleurotus ostreatus. White-rot fungus P. ostreatus was isolated from maize silage (MS) and thereafter it was inoculated on MS as substrate and compared with maize stover (MSt) and maize cobs (MC) to determine the best lignocellulosic substrate for the production of lignocellulolytic enzymes and extracellular protein. The MS gave the highest productivity of CMCase (368.2 U/mL), FPase (170.5 U/mL), laccase (11.4 U/mL), and MnPase (6.6 U/mL). This is compared to productivity on MSt of 222 U/mL, 50.2 U/mL, 4.55 U/mL, and 2.57 U/mL, respectively; and productivity on MC at the same incubation period as 150.5 U/mL, 48.2 U/mL, 3.58 U/mL, and 2.5 U/mL, respectively. The levels of enzyme production declined with increasing incubation period after 15 and 20 days using MS and MC, respectively, as substrates. Maximum liberated extracellular protein content (754 to 878 µg/mL) was recorded using MS, while a low amount (343 to 408 µg/mL) was liberated with using MSt and MC.

Transformation of lamotrigine by white-rot fungus Pleurotus ostreatus

2019 ◽  
Vol 250 ◽  
pp. 546-553 ◽  
Author(s):  
Benny Chefetz ◽  
Rotem Marom ◽  
Orit Salton ◽  
Mariana Oliferovsky ◽  
Vered Mordehay ◽  
...  
Keyword(s):  
Pleurotus Ostreatus ◽  
White Rot ◽  
White Rot Fungus

scholarly journals Trace Metal Detection in Aqueous Reservoirs Using Stilbene Intercalated Layered Rare-Earth Hydroxide Tablets

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
Solomon Omwoma
Keyword(s):  
Trace Metal ◽  
Metal Ions ◽  
Metal Ion ◽  
Aqueous Media ◽  
Mitigation Measures ◽  
Metal Ion Analysis ◽  
Gradual Process ◽  
Metal Detection ◽  
Trace Metal Detection ◽  
High Concentrations

Contamination of aquatic reservoirs with metal ions is a slow gradual process that is not easy to detect. Consequences of the metal ions, especially the ones with high atomic numbers (heavy metals) at high concentrations, are severe and irreversible in aquatic reservoirs. As such, early detection mechanisms, especially at trace concentration, are essential for mitigation measures. In this work, a new, robust, and effective tool for trace metal detection and monitoring in aqueous solutions has been developed. Tablets (1 mm thick and similar to medicinal tablets) were manufactured from a powder comprising stilbene intercalated into gallery spaces of lanthanide-containing layered double hydroxides. The tablets were placed in a water column having different concentrations of Pb2+ and Cu2+ ions, and the water was allowed to flow for 45 minutes at a flow rate of 100 ml/s. Thereafter, the tablets were dried and made to powder, and their phosphorescence was measured. The gradual stilbene phosphorescence turnoff in the tablets from various concentrations of metal ions was correlated with sorption amounts. The tablets were able to detect effectively metal ions (up to Pb2+ 1.0 mmol/L and Cu2+ 5.0 mmol/L) in the aqueous media. As such, the concentrations of Pb2+ and Cu2+ ions at trace levels were determined in the test solutions. This method provides a real-time metal ion analysis and does not involve sampling of water samples for analysis in the laboratory.

scholarly journals Extracellular Biosynthesis of Copper and Copper Oxide Nanoparticles byStereum hirsutum, a Native White-Rot Fungus from Chilean Forests

2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
R. Cuevas ◽  
N. Durán ◽  
M. C. Diez ◽  
G. R. Tortella ◽  
O. Rubilar
Keyword(s):  
Copper Oxide ◽  
Fourier Transforms ◽  
Copper Oxide Nanoparticles ◽  
White Rot ◽  
White Rot Fungus ◽  
Oxide Nanoparticles ◽  
Copper Salts ◽  
Tem Analysis ◽  
Extracellular Biosynthesis ◽  
Alkaline Conditions

The white-rot fungusStereum hirsutumwas studied to evaluate its applicability for use in the biosynthesis of copper/copper oxide nanoparticles under different pH conditions and in the presence of three different copper salts (CuCl2, CuSO4, and Cu(NO3)2). The nanoparticle formation was evaluated by UV-visible spectroscopy, electron microscopy (TEM), X-ray diffraction analysis (XRD), and Fourier transforms infrared spectroscopy (FTIR). The nanoparticles biosynthesis in presence of all copper salts demonstrated higher formation with 5 mM CuCl2under alkaline conditions. TEM analysis confirmed that the nanoparticles were mainly spherical (5 to 20 nm). The presence of amine groups attached to nanoparticles was confirmed by FTIR, which suggests that extracellular protein of fungus is responsible for the formation of the nanoparticles. Therefore, the white-rot fungusS. hirsutumwas found to exhibit potential for use in the synthesis of copper/copper oxide nanoparticles.

Exclusive overproduction of recombinant versatile peroxidase MnP2 by genetically modified white rot fungus, Pleurotus ostreatus

2006 ◽  
Vol 126 (4) ◽  
pp. 431-439 ◽  
Author(s):  
Takahisa Tsukihara ◽  
Yoichi Honda ◽  
Ryota Sakai ◽  
Takahito Watanabe ◽  
Takashi Watanabe
Keyword(s):  
Pleurotus Ostreatus ◽  
Genetically Modified ◽  
White Rot ◽  
Versatile Peroxidase ◽  
White Rot Fungus
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