scholarly journals Extracellular Biosynthesis of Copper and Copper Oxide Nanoparticles byStereum hirsutum, a Native White-Rot Fungus from Chilean Forests

2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
R. Cuevas ◽  
N. Durán ◽  
M. C. Diez ◽  
G. R. Tortella ◽  
O. Rubilar

The white-rot fungusStereum hirsutumwas studied to evaluate its applicability for use in the biosynthesis of copper/copper oxide nanoparticles under different pH conditions and in the presence of three different copper salts (CuCl2, CuSO4, and Cu(NO3)2). The nanoparticle formation was evaluated by UV-visible spectroscopy, electron microscopy (TEM), X-ray diffraction analysis (XRD), and Fourier transforms infrared spectroscopy (FTIR). The nanoparticles biosynthesis in presence of all copper salts demonstrated higher formation with 5 mM CuCl2under alkaline conditions. TEM analysis confirmed that the nanoparticles were mainly spherical (5 to 20 nm). The presence of amine groups attached to nanoparticles was confirmed by FTIR, which suggests that extracellular protein of fungus is responsible for the formation of the nanoparticles. Therefore, the white-rot fungusS. hirsutumwas found to exhibit potential for use in the synthesis of copper/copper oxide nanoparticles.

2011 ◽  
Vol 14 (3) ◽  
pp. 61-69
Author(s):  
Hanh Ngoc Nguyen ◽  
Thao Huu Vo

Nanoparticles of metal and metallic oxides have become a very active research area in the field of material chemistry. The surface effect is mainly responsible for deviation of the properties of nano-materials from that of the bulk. Nanosize copper oxide was synthesized by hydrolysis of copper salts in basic medium using biodegradable non-ionic polymer polyethylene glycol (PEG) as surface active agent The X-ray powder diffraction patterns (XRD) present typical peaks of copper oxides formed. The transmission electron microscopy (TEM) and scanning electron microscopy (SEM) images determined the shape and the nanosizes of the particles of about 10-30nm. The results exhibited the role of intermediate nanosize copper hydroxide species on the formation of copper oxide nanoparticles. The influence of synthesis temperature, reaction time, calcination temperature, etc. was studied.


2020 ◽  
Vol 10 (03) ◽  
pp. 378-382
Author(s):  
Jenan Hussien Taha ◽  
Nada Khudair Abbas ◽  
Azhar A. F. Al-Attraqchi

In this article, a simple new technique for the green synthesis of copper oxide nanoparticles (CuO NPs) using peroxidases oxidoreductases (POX) enzyme extracted from fig leaves for antifungal and antibacterial activities has been reported. Subsequently, a comprehensive investigation of the structural, optical, and morphological properties of the synthesized CuO NPs was elucidated, using X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), Electrodiagnostic (EDX), atomic force microscopy (AFM), and transmission electron microscopy (TEM) analysis techniques. Specifically, the resultant nanoparticles are spherical with a diameter ranging from 28–68. CuO NPs were further tested for their antifungal activity against Candida and Aspergillus species, while the antibacterial activity was screened in contradiction of pathogenic bacterial strains namely gram-positive Staphylococcus aureus and gram-negative Asinobacterial species. The present study reveals a convenient use of POX fig leaves extract as fuel, for the well-organized synthesis of CuO NPs via green synthesis technique to acquire considerably active antifungal and antibacterial materials.


Author(s):  
Haider Qassim Raheem ◽  
Takwa S. Al-meamar ◽  
Anas M. Almamoori

Fifty specimens were collected from wound patients who visited Al-Hilla Teaching Hospital. The samples were grown on Blood and MacConkey agar for 24-48 hr at 37oC. The bacterial isolates which achieved as a pure and predominant growth from clinical samples as Pseudomonas fluorescens, were identified using morphological properties and Vitek2 system. The anti-bacterial activity of copper oxide nanoparticles (CuO NPs) against was tested by (disk diffusion assay) using dilutions of (400, 200, 100, 50, 25, and 12.5‎µ‎g/ml). The (MIC and MBC) of each isolate was determined. CuO NPs shows wide spectrum antibacterial activity against tested bacteria with rise zone of inhibition diameter that is proportionate with the increase in nanoparticle concentration. The MIC of CuO NPs extended from 100-200‎µ‎g/ml and the MBC ranged from 200-400‎µ‎g/ml. The antibiotic profile was determined by Viteck 2 compact system (Biomérieux). CuO NPs‎ found highly effective and safe in P. fluorescens wounds infections comparing with used antibiotics.


2020 ◽  
Vol 3 (3) ◽  
Author(s):  
Hemalatha D ◽  
Saraswath S

In material science, green method for synthesis of nanomaterials is feasible, cheaper and eco-friendly protocol. To accomplish this phenomenon, present study was aimed to synthesize Copper oxide nanoparticles using leaf extract of Aloevera with two different precursors CuCl2.2H2O (Cupric chloride) and CuSo4.5H2O (Cupric sulfate). The extraction of Aloevera is employed as reducing and stabilizing agent for this synthesis.Copper oxide Nanoparticles is effective use of biomedical application due to their antibacterial function. The synthesized Copper oxide nanoparticles were characterized by X-Ray Diffraction Spectroscopy (XRD), Energy Dispersive Spectroscopy (EDX), FourierTransform Infrared Spectroscopy (FT- IR) and Scanning Electron Microscope(SEM). XRD studies reveal the crystallographic nature of Copper oxide nanoparticles. Furthermore the Copper oxide nanoparticles have good Antibacterial activity against both gram negative (E.Coli, Klebsiella pneumonia) and gram positive (Bacillus cereus, Staphylococcus aureus)bacteria.


Author(s):  
Monika Vats ◽  
Shruti Bhardwaj ◽  
Arvind Chhabra

Background & Objective: Nanoparticles are used in cosmetic and dermatologic products, due to better skin penetration properties. Incorporation of natural products exhibiting medicinal properties in nano-preparations could significantly improve efficacy of these products and improve the quality of life without the side effects of synthetic formulations. Methods: We here report green synthesis of Copper Oxide nanoparticles, using Cucumber extract, and their detailed biophysical and bio-chemical characterization. Results: These Copper Oxide-Cucumber nanoparticles exhibit significant anti-bacterial and anti-fungal properties, Ultra Violet-radiation protection ability and reactive-oxygen species inhibition properties. Importantly, these nanoparticles do not exhibit significant cellular toxicity and, when incorporated in skin cream, exhibit skin rejuvenating properties. Conclusion: Our findings have implications for nanoparticle-based cosmetics and dermatologic applications.


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