scholarly journals Recent Progress in Small Spirocyclic, Xanthene-Based Fluorescent Probes

Molecules ◽  
2020 ◽  
Vol 25 (24) ◽  
pp. 5964
Author(s):  
Sascha G. Keller ◽  
Mako Kamiya ◽  
Yasuteru Urano
Keyword(s):  
Small Molecules ◽  
Fluorescent Probes ◽  
Recent Progress ◽  
Super Resolution ◽  
Short Introduction ◽  
Computational Approaches ◽  
Recent Advances ◽  
Silicon Carbon ◽  
Super Resolution Microscopy ◽  
Made In

The use of fluorescent probes in a multitude of applications is still an expanding field. This review covers the recent progress made in small molecular, spirocyclic xanthene-based probes containing different heteroatoms (e.g., oxygen, silicon, carbon) in position 10′. After a short introduction, we will focus on applications like the interaction of probes with enzymes and targeted labeling of organelles and proteins, detection of small molecules, as well as their use in therapeutics or diagnostics and super-resolution microscopy. Furthermore, the last part will summarize recent advances in the synthesis and understanding of their structure–behavior relationship including novel computational approaches.

scholarly journals Recent Advances in Organelle-Targeted Fluorescent Probes

Molecules ◽  
2021 ◽  
Vol 26 (1) ◽  
pp. 217
Author(s):  
Na-Eun Choi ◽  
Ji-Yu Lee ◽  
Eun-Chae Park ◽  
Ju-Hee Lee ◽  
Jiyoun Lee
Keyword(s):  
Fluorescent Probes ◽  
Molecular Level ◽  
Imaging Techniques ◽  
Super Resolution ◽  
Structural Features ◽  
Future Directions ◽  
Cellular Processes ◽  
Biochemical Pathways ◽  
Recent Advances ◽  
Super Resolution Microscopy

Recent advances in fluorescence imaging techniques and super-resolution microscopy have extended the applications of fluorescent probes in studying various cellular processes at the molecular level. Specifically, organelle-targeted probes have been commonly used to detect cellular metabolites and transient chemical messengers with high precision and have become invaluable tools to study biochemical pathways. Moreover, several recent studies reported various labeling strategies and novel chemical scaffolds to enhance target specificity and responsiveness. In this review, we will survey the most recent reports of organelle-targeted fluorescent probes and assess their general strategies and structural features on the basis of their target organelles. We will discuss the advantages of the currently used probes and the potential challenges in their application as well as future directions.

Recent advances in luminescent materials for super-resolution imaging via stimulated emission depletion nanoscopy

2021 ◽  
Author(s):  
Yanzi Xu ◽  
Ruohan Xu ◽  
Zhi Wang ◽  
Yu Zhou ◽  
Qifei Shen ◽  
...  
Keyword(s):  
Recent Progress ◽  
Super Resolution ◽  
Stimulated Emission ◽  
Luminescent Materials ◽  
New Materials ◽  
Recent Advances ◽  
Resolution Imaging ◽  
Stimulated Emission Depletion

Recent progress on STED fluorophores for super-resolution imaging and also their characteristics are outlined here, thus providing some guidelines to select proper probes and even develop new materials for super-resolution imaging via STED nanoscopy.

Small-Molecule Fluorescent Probes for Live-Cell Super-Resolution Microscopy

2018 ◽  
Vol 141 (7) ◽  
pp. 2770-2781 ◽  
Author(s):  
Lu Wang ◽  
Michelle S. Frei ◽  
Aleksandar Salim ◽  
Kai Johnsson
Keyword(s):  
Small Molecule ◽  
Fluorescent Probes ◽  
Super Resolution ◽  
Live Cell ◽  
Super Resolution Microscopy

scholarly journals Intracellular Delivery of Membrane Impermeable Photostable Fluorescent Probes into Living Cells for Super-Resolution Microscopy

2017 ◽  
Vol 112 (3) ◽  
pp. 21a
Author(s):  
Yuji Ishitsuka ◽  
Kai Wen Teng ◽  
Pin Ren ◽  
Yeoan Youn ◽  
Xiang Deng ◽  
...  
Keyword(s):  
Fluorescent Probes ◽  
Super Resolution ◽  
Intracellular Delivery ◽  
Living Cells ◽  
Super Resolution Microscopy

scholarly journals Fluorescent Probes for Super-Resolution Microscopy of Lysosomes

ACS Omega ◽  
2020 ◽  
Vol 5 (42) ◽  
pp. 26967-26977
Author(s):  
Aditya Yadav ◽  
Chethana Rao ◽  
Chayan K. Nandi
Keyword(s):  
Fluorescent Probes ◽  
Super Resolution ◽  
Super Resolution Microscopy

scholarly journals Fluorescent Probes for STED Optical Nanoscopy

Nanomaterials ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 21
Author(s):  
Sejoo Jeong ◽  
Jerker Widengren ◽  
Jong-Chan Lee
Keyword(s):  
Fluorescent Probes ◽  
Organic Dyes ◽  
Fluorescent Proteins ◽  
Photophysical Properties ◽  
Optical Resolution ◽  
Super Resolution ◽  
Fluorescent Nanoparticles ◽  
Resolution Limit ◽  
Photochemical Properties ◽  
Super Resolution Microscopy

Progress in developing fluorescent probes, such as fluorescent proteins, organic dyes, and fluorescent nanoparticles, is inseparable from the advancement in optical fluorescence microscopy. Super-resolution microscopy, or optical nanoscopy, overcame the far-field optical resolution limit, known as Abbe’s diffraction limit, by taking advantage of the photophysical properties of fluorescent probes. Therefore, fluorescent probes for super-resolution microscopy should meet the new requirements in the probes’ photophysical and photochemical properties. STED optical nanoscopy achieves super-resolution by depleting excited fluorophores at the periphery of an excitation laser beam using a depletion beam with a hollow core. An ideal fluorescent probe for STED nanoscopy must meet specific photophysical and photochemical properties, including high photostability, depletability at the depletion wavelength, low adverse excitability, and biocompatibility. This review introduces the requirements of fluorescent probes for STED nanoscopy and discusses the recent progress in the development of fluorescent probes, such as fluorescent proteins, organic dyes, and fluorescent nanoparticles, for the STED nanoscopy. The strengths and the limitations of the fluorescent probes are analyzed in detail.

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