scholarly journals The Effect of Nitrogen Fertigation and Harvesting Time on Plant Growth and Chemical Composition of Centaurea raphanina subsp. mixta (DC.) Runemark

Molecules ◽  
2020 ◽  
Vol 25 (14) ◽  
pp. 3175 ◽  
Author(s):  
Spyridon A. Petropoulos ◽  
Ângela Fernandes ◽  
Maria Inês Dias ◽  
Carla Pereira ◽  
Ricardo C. Calhelha ◽  
...  

The aim of the present study was to evaluate the effect of nitrogen fertigation (0, 200, 400, and 600 ppm of total nitrogen) and harvesting time (9 March 2018 and 19 April 2018) on the plant growth, chemical composition, and bioactive properties of Centaurea raphanina subsp. mixta plants. The highest yield of fresh leaves was observed for the treatment of 200 ppm of N without compromising nutritional value. The increasing nitrogen levels resulted in an increase of α- and total tocopherols and sugars content, especially in the second harvest for tocopherols and in the first harvest for sugars. Similarly, total organic acids and oxalic acid content increased with increasing nitrogen levels in both harvests, while fatty acids composition had a varied response to the tested factors. Pinocembrin neohesperidoside and pinocembrin acetyl neohesperidoside isomer II were the most abundant phenolic compounds with the highest content being observed in the control treatment of the first and second harvest, respectively. The highest antioxidant activity was observed for the control and the 600 ppm treatments of the second harvest for the OxHLIA and TBARS assays, respectively, probably due to the high content of pinocembrin acetyl neohesperidoside isomer II and α-tocopherol, respectively. Finally, cytotoxic effects and antimicrobial properties showed a varied response depending on the treatment. In conclusion, C. raphanina subsp. mixta has low requirements of nitrogen to achieve the highest yield, while a varied response to the tested fertigation treatments and harvesting time was observed in terms of the chemical composition and the bioactive properties.

Molecules ◽  
2020 ◽  
Vol 25 (9) ◽  
pp. 2204 ◽  
Author(s):  
Spyridon A. Petropoulos ◽  
Ângela Fernandes ◽  
Maria Ines Dias ◽  
Carla Pereira ◽  
Ricardo C. Calhelha ◽  
...  

The aim of this report was to study the effect of salinity (control: 2dS/m, S1: 4 dS/m and S2: 6 dS/m) and harvest time (first harvest on 9 May 2018 and second harvest on 19 April 2018) on the growth and the chemical composition of Centaurea raphanina subsp. mixta plants. The plants of the first harvest were used for the plant growth measurements (fresh weight and moisture content of leaves, rosette diameter, number and thickness of leaves), whereas those of the second harvest were not used for these measurements due to the flowering initiation, which made the leaves unmarketable due to their hard texture. The results of our study showed that C. raphanina subsp. mixta plants can be cultivated under mild salinity (S1 treatment) conditions without severe effects on plant growth and yield, since a more severe loss (27.5%) was observed for the S2 treatment. In addition, harvest time proved to be a cost-effective cultivation practice that allows to regulate the quality of the final product, either in edible form (first harvest) or for nutraceutical and pharmaceutical purposes as well as antimicrobial agents in food products. Therefore, the combination of these two agronomic factors showed interesting results in terms of the quality of the final product. In particular, high salinity (S2 treatment) improved the nutritional value by increasing the fat, proteins and carbohydrates contents in the first harvest, as well as the tocopherols and sugars contents (S1 and S2 treatments, respectively) in the second harvest. In addition, salinity and harvest time affected the oxalic acid content which was the lowest for the S2 treatment at the second harvest. Similarly, the richest fatty acid (α-linolenic acid) increased with increasing salinity at the first harvest. Salinity and harvest time also affected the antimicrobial properties, especially against Staphylococcus aureus, Bacillus cereus and Trichoderma viride, where the extracts from the S1 and S2 treatments showed high effectiveness. In contrast, the highest amounts of flavanones (pinocembrin derivatives) were detected in the control treatment (second harvest), which was also reflected to the highest antioxidant activity (TBARS) for the same treatment. In conclusion, C. raphanina subsp. mixta plants seem to be tolerant to medium salinity stress (S1 treatment) since plant growth was not severely impaired, while salinity and harvesting time affected the nutritional value (fat, proteins, and carbohydrates) and the chemical composition (tocopherols, sugars, oxalic acid, fatty acids), as well as the bioactive properties (cytotoxicity and antimicrobial properties) of the final product.


2017 ◽  
Vol 8 (12) ◽  
pp. 4733-4743 ◽  
Author(s):  
Spyridon Petropoulos ◽  
Ângela Fernandes ◽  
Lillian Barros ◽  
Ana Ciric ◽  
Marina Sokovic ◽  
...  

Okra seeds present significant nutritional value and antimicrobial properties and could be proposed for innovative okra products and functional foods with antimicrobial and bioactive properties.


Agronomy ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1416 ◽  
Author(s):  
Ângela Fernandes ◽  
Nikolaos Polyzos ◽  
Spyridon A. Petropoulos ◽  
José Pinela ◽  
Elizandra Ardohain ◽  
...  

In the present study plant growth, nutritional value and chemical composition of leaves from twenty-five plain-leafed, curly-leafed and turnip-rooted parsley cultivars were evaluated. Total fresh yield was higher for the plain-leafed cv. Rialto Bejo: 192 ± 11 g/pot, while significant differences were observed between the three types in the nutritional parameters, except for the carbohydrates content. The most abundant organic acid was malic acid (5.22–6.88 g/100 g dw), while the total sugars content did not differ significantly among the tested cultivar types. α-tocopherol was the major tocopherol detected in amount that ranged between 14.76–30.32 mg/100 g dw. The main fatty acids were α-linolenic and linoleic followed by palmitic acid, while only linoleic acid content being different among the cultivar types. In conclusion, the existing diversity in the parsley genotypes could be valorised to increase the agrobiodiversity in the broader Mediterranean region through the introduction of less cultivated curly-leafed and turnip-rooted types.


2020 ◽  
Vol 11 (4) ◽  
pp. 2969-2977 ◽  
Author(s):  
Shirley L. Sampaio ◽  
Ângela Fernandes ◽  
Carla Pereira ◽  
Ricardo C. Calhelha ◽  
Marina Sokovic ◽  
...  

Quinoa is a very interesting food due to its nutritional and chemical composition, as well as its bioactive properties, such as antioxidant and antimicrobial activity.


Agronomy ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 474 ◽  
Author(s):  
Spyridon A. Petropoulos ◽  
Ângela Fernandes ◽  
Nikolaos Polyzos ◽  
Vasileios Antoniadis ◽  
Lillian Barros ◽  
...  

Potato cultivation is quite demanding in inorganic nutrients and adequate fertilization is a key factor for maximizing yield and producing tubers of high quality. In the present study, a field experiment was carried out to evaluate the effect of various forms of fertilization on crop performance and the nutritional value and chemical composition of two potato varieties (cv. Spunta and cv. Kennebec). For this purpose, five different fertilizer treatments were applied namely: control (C), standard fertilizer (T1), standard fertilizer + zeolite (T2), manure (T3) and slow release nitrogen fertilizer (T4). According to the results, it was observed that slow release treatment (T4) achieved the highest yield for both varieties, while the control treatment presented significantly lower yield compared to the studied fertilization regimes. The dry matter of leaves and shoots was higher in T1 treatment for cv. Kennebec and in T2 and T4 treatments for cv. Spunta, whereas the control treatment presented the highest dry matter content in tubers for cv. Kennebec and T2 and T3 treatments for cv. Spunta. A significant effect of the fertilization regime was also observed on the nutritional value of tubers and more specifically the protein, ash and fat content was increased by treatments T1 and T4, while carbohydrate content was also increased by T3 and T4 treatments for both varieties. Similarly, the total sugars, organic acids, β-carotene and lycopene content was increased in T3 treatment for the Spunta variety, while the antioxidant capacity showed a varied response depending on the fertilizer regime and the tested variety. In conclusion, the fertilization regime has a significant effect not only on the tuber yield but also on the quality of the final product and should be considered as an effective tool to increase the added value of potato crop.


Agronomy ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 715 ◽  
Author(s):  
Spyridon A. Petropoulos ◽  
Ângela Fernandes ◽  
Eustratios Xyrafis ◽  
Nikolaos Polyzos ◽  
Vasileios Antoniadis ◽  
...  

In this study, we evaluated the effect of various fertilization regimes on processing tomato (Solanum lycopersicum L. cv. Heinz 3402) yield and quality by applying the following treatments: (i) control (C), (ii) conventional fertilizer (21-0-0, N-P-K) (CF), (iii) slow release nitrogen fertilizer 46-0-0 (SR), (iv) conventional fertilizer (21-0-0, N-P-K) + Zeolite (CFZ), and v) composted sheep manure (M). The results of the study showed that the SR and CFZ treatments resulted in the highest fruit yield per hectare compared to the rest of the fertilizer and the control treatments. Fruit firmness was higher for the treatments C, M and SR, while color parameters (chroma and hue angle) were higher for the C and M treatments, respectively. Moreover, the total soluble solids content (TSS; °Brix) was higher when manure (M) was applied. In terms of chemical composition, the total and individual tocopherols and sugars were the highest for the M and C treatments, respectively, whereas the oxalic, malic and total organic acid contents were the highest for the CFZ treatment. Moreover, the tested treatments showed a varied response in different antioxidant assays, although the M treatment exhibited a high antioxidant capacity in most of the assays, except for the β-carotene/linoleate assay. The carotenoid and chlorophyll contents were the highest for the control treatment. The main detected fatty acid was linoleic acid, followed by palmitic, oleic and α-linolenic acid, while the CFZ treatment had the highest content of polyunsaturated fatty acids (PUFA) due to its high content of linoleic acid. In conclusion, although the application of fertilizers increased yield, the quality parameters and chemical composition showed a varied response to the fertilization regime, especially the TSS content and juice pH and electric conductivity (EC), which are significant for the marketability of the final product.


Agronomy ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 576
Author(s):  
Spyridon A. Petropoulos ◽  
Ângela Fernandes ◽  
Maria Inês Dias ◽  
Carla Pereira ◽  
Ricardo C. Calhelha ◽  
...  

The Mediterranean basin is abundant in wild edible species with numerous health beneficial effects due to the presence of various bioactive phytochemicals. In the present work, the effect of nitrogen fertilization rates (0 ppm, (N0), 200 ppm (N1), 400 ppm (N2), and 600 ppm (N3) of total N) and growth substrate composition (soil or peat/perlite (2/1; v/v)) on the chemical composition and bioactive properties of Centaurea raphanina ssp. mixta plants was evaluated. The results of the study showed that both the tested factors affected nutritional value of the edible leaves, with the soil × N1 treatment being the most beneficial for fat, protein, and carbohydrate content and energetic value. On the other hand, the peat/perlite-grown plants that received 200 ppm of N had the highest content in α-, γ-, and total tocopherols, while the control treatment of soil-grown plants was the richest in individual and total sugars. Oxalic, citric, and total organic acids were the highest in the N2 × soil treatment, while malic acid was the highest in control treatment of the same substrate. The main fatty acids were palmitic, α-linolenic, and linoleic acids, with the highest contents being observed in the N0 × soil, N3 × soil, and N3 × peat/perlite treatments, respectively. The major phenolic compounds were pinocembrim neohesperidoside and pinocembrim acetyl neohesperidoside isomer II, with the highest content being observed in the N1 × soil treatment. The highest antihemolytic activity was observed in the N3 × peat/perlite treatment, while the most effective treatments against lipid peroxidation were N0 (in both soil and peat/perlite combinations) and N1 × peat/perlite. Lastly, all the tested extracts (except for N1 × soil) showed promising cytotoxic effects against HeLa (cervical carcinoma), HepG2 (hepatocellular carcinoma), MCF-7 (breast carcinoma), and NCI-H460 (non-small-cell lung cancer), while all the tested extracts exhibited better antifungal activities (lower minimal inhibition concentration (MIC) values) against Trichoderma viride than the positive controls. Overall, the present results suggest that the application of cost-effective practices such as the nitrogen application and the selection of growth substrate may regulate the chemical composition and the bioactive properties of C. raphanina ssp. mixta species and increase its added value under commercial cultivation conditions.


Author(s):  
Amário Nuno Meireles Duarte ◽  
Cecílio Viega Soares Filho ◽  
Marcelo Carvalho Minhoto Teixeira Filho ◽  
Caroline Lopes Monteiro de Carvalho ◽  
Mariangela Hungria ◽  
...  

Strategies that improve the use of nitrogen (N), by tropical forage grasses, can bring environmental and social gains. Thus, this study aimed to evaluate the forage productivity and the nutritional value (NV) of the hybrid Urochloa spp. cv. ´Mavuno`, under inoculation with plant growth-promoting bacteria (PGPB) and doses of N. The experiment was carried out in the field, for a period of 14 months, in a randomized block design, with nine treatments and four replications in plots of 9 m². Seed inoculations were tested with: Azospirillum brasilense Ab-V5 and Ab-V6 strains; Pseudomonas fluorescens CCTB 03 and co-inoculation with Rhizobium tropici CIAT 899 and A. brasilense Ab-V6, combined with 50 and 100 kg ha-1 of added mineral N; fertilization with N-mineral with these doses, without inoculation and the control treatment, without N and without inoculation. The bacteria were inoculated to the grass seeds. The forage was evaluated by determining the shoot dry weight yield (SDWY) and the levels of crude protein (CP), neutral detergent insoluble fiber (NDF), acid detergent insoluble fiber (ADF) and in vitro dry matter digestibility (IVDMD). The results were subjected to analysis of variance by the F test (p ≤ 0.05) and compared to each other by the Scott-Knott test at 5% probability. Inoculation with PGPB resulted in greater SDWY when associated with a dose of 50 kg ha-1 of N. In plants inoculated with A. brasilense Ab-V5 + Ab-V6 and P. fluorescens CCTB 03, associated with a dose of 50 kg ha-1 of N-mineral, SDWY was similar to that obtained with double the dose of N in the absence of inoculation. The inoculation with PGPB resulted in an increase in the NV of Mavuno grass, with emphasis on A. brasilense Ab-V5 + Ab-V6 and P. fluorescens CCTB 03, associated with the dose of 100 kg ha-1 of N-mineral that improved the nutritional value in all evaluated items. The inoculation with A. brasilense Ab-V5 + Ab-V6 was the most promising, followed by P. fluorescens CCTB 03, mainly in association with 50 kg ha-1 of N.


Agronomy ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 344
Author(s):  
Jens Torsten Mackens ◽  
Bruno Maximilian Görlach ◽  
Karl-Hermann Mühling

Biogas residues (BGR) contain a variety of plant nutrients and are, therefore, valuable fertilizers. However, ammonia (NH3) emissions occur during slurry and BGR application. These emissions can be reduced by lowering the pH of the BGR. Acidification technology works well for slurry, but little is known about the effects on fertilizer properties of acidified BGR (ABGR). This study aimed to examine the impact of acidification on the chemical composition of BGR and its influence on plant growth of juvenile maize and the soil pH, as well as the soluble soil phosphorous (P) and manganese (Mn), after application of ABGR. The soluble amount of nutrients in BGR was compared with that in ABGR. In an outdoor pot experiment, BGR and ABGR were incorporated in soil, and maize was grown for 8 weeks. Two different BGR P levels were compared with (NH4)2HPO4 and a control treatment without additional P. BGR acidification increased dissolved amounts of P from 15% to 44%, calcium from 6% to 59%, magnesium from 7% to 37%, and Mn from 2% to 15%. The dry matter of ABGR-fertilized maize was 34%, 45% higher than that of BGR-fertilized maize. The soluble Mn content in the soil was 74% higher with the low ABGR dose and 222% higher with the higher ABGR dose than the BGR treatments. The fertilizer efficiency of ABGR was higher than that of BGR, indicating that the absolute amount of applied fertilizer could be reduced in systems using ABGR.


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