scholarly journals Sustainable Micro-Scale Extraction of Bioactive Phenolic Compounds from Vitis vinifera Leaves with Ionic Liquid-Based Surfactants

Molecules ◽  
2020 ◽  
Vol 25 (13) ◽  
pp. 3072 ◽  
Author(s):  
Giulia Mastellone ◽  
Idaira Pacheco-Fernández ◽  
Patrizia Rubiolo ◽  
Verónica Pino ◽  
Cecilia Cagliero
Keyword(s):  
Ionic Liquid ◽  
Phenolic Compounds ◽  
Vitis Vinifera ◽  
High Performance ◽  
Photodiode Array Detection ◽  
Micro Scale ◽  
Simple Strategy ◽  
Analytical System ◽  
Solid Liquid ◽  
Extraction Phase

This paper proposes a new sustainable and simple strategy for the micro-scale extraction of phenolic compounds from grapevine leaves with analytical purpose. The method is based on a microwave-assisted solid-liquid extraction approach (MA-SLE), using an aqueous solution of an ionic liquid (IL)-based surfactant as extraction phase. The method does not require organic solvents, nor any clean-up step, apart from filtration prior to the injection in the analytical system. Two IL-based surfactants were evaluated, and the method was optimized by using experimental designs, resulting in the use of small amounts of sample (100 mg) and extraction phase (2.25 mL), low concentrations of the selected 1-hexadecyl-3-butyl imidazolium bromide IL (0.1 mM), and 30 min of extraction time. The proposed methodology was applied for the determination of the polyphenolic pattern of six different varieties of Vitis vinifera leaves from the Canary Islands, using high-performance liquid chromatography and photodiode array detection for the quantification of the compounds. The proposed MA-SLE approach was greener, simpler, and more effective than other methods, while the results from the analysis of the leaves samples demonstrate that these by-products can be exploited as a source of natural compounds for many applications.

scholarly journals Determination of major anthocyanin pigments and flavonols in red grape skin of some table grape varieties (Vitis vinifera sp.) by high-performance liquid chromatography–photodiode array detection (HPLC-DAD)

OENO One ◽  
2016 ◽  
Vol 50 (3) ◽  
Author(s):  
Farida Benmeziane ◽  
Yves Cadot ◽  
Rachid Djamai ◽  
Lynda Djermoun
Keyword(s):  
Vitis Vinifera ◽  
High Performance ◽  
Natural Antioxidants ◽  
Reversed Phase ◽  
Table Grape ◽  
Photodiode Array Detection ◽  
Grape Skin ◽  
Total Anthocyanins ◽  
Red Grape ◽  
Grape Varieties

<p style="text-align: justify;"><strong>Aim</strong>: The aim of this study was the investigation of the anthocyanin and flavonol content in grape skin extracts. Five prevalent anthocyanin-types and four flavonol-types were determined in the skin of three red table grape varieties widely cultivated in El-Tarf (Algeria).</p><p style="text-align: justify;"><strong>Methods and results</strong>: The identification of the compounds was performed by HPLC-DAD based on C-18 reversed phase column separation. Results from HPLC analysis showed that malvidin and petunidin-3-<em>O</em>-glucoside were the major anthocyanin glucoside, whereas quercetin-3-<em>O</em>-glucoside was the major flavonol among the four identified.</p><p style="text-align: justify;"><strong>Conclusion</strong>: The content of anthocyanins and flavonols in the grape skin of three grapevine (<em>Vitis vinifera</em>) varieties exhibits notable differences among the cultivars studied, confirming their importance in the varietal characterization. The highest concentrations of total anthocyanins and flavonols corresponded to the Gros noir variety. The results of the present study also indicate that the grape skin extracts of these Algerian cultivars can be used as easily accessible source of natural antioxidants.  </p><strong>Significance and impact of the study</strong>: To the best of our knowledge, this is the first report on the identification of different anthocyanins and flavonols in berry skin from some red grape varieties largely cultivated in this region of Algeria.

scholarly journals Influence of Different Solvents on the Phenolic Compounds Content Grape Bark Extracts (Vitis vinifera) from Syrah Variety

2020 ◽  
Vol 2 (3) ◽  
pp. 105-110
Author(s):  
Euzélia Lima de Souza1 ◽  
Talita Sousa Nascimento ◽  
Gabriele De Abreu Barreto ◽  
Ingrid Lessa Leal ◽  
Jeancarlo Pereira dos Anjos ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Phenolic Compounds ◽  
Vitis Vinifera ◽  
High Performance ◽  
Wine Industry ◽  
Standard Curve ◽  
Grain Alcohol ◽  
Grape Peel

Grape bark from the wine industry still contains significant amounts of phenolic compounds. Specific characteristics of the applied solvents influence the extraction of phenolic compounds directly. This study aimed to evaluate the influence of different solvents obtaining phenolic compounds in Syrah grape barkextracts by High-Performance Liquid Chromatography (HPLC). Grape peel extracts were prepared with three different solvents (water, 50%, and 80% grain alcohol), and evaluated using standard curve with 15 phenolic compounds. It was not possible to quantify the phenolic compounds, but the chromatogramssuggest the presence of some of them. The solvent that provided the best interaction with grape peel was 50% grain alcohol.

scholarly journals LC-MS/MS-QTOF Screening and Identification of Phenolic Compounds from Australian Grown Herbs and Their Antioxidant Potential

Antioxidants ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 1770
Author(s):  
Akhtar Ali ◽  
Yasmeen M. Bashmil ◽  
Jeremy J. Cottrell ◽  
Hafiz A. R. Suleria ◽  
Frank R. Dunshea
Keyword(s):  
Liquid Chromatography ◽  
Phenolic Compounds ◽  
High Performance ◽  
Antioxidant Activities ◽  
Positive Impact ◽  
Total Phenolic ◽  
Fragmentation Pattern ◽  
Photodiode Array Detection ◽  
Potential Health

Culinary spices and herbs have been used to impart a characteristic flavour and aroma in food due to their appealing fragrance. Recently, bioactive compounds from herbs, especially phenolics, have gained much attention due to their potential health outcomes. The aim of this study was to characterize and quantify the phenolic compounds from 10 widely used Australian-grown herbs (oregano, rosemary, bay, basil, sage, fenugreek, dill, parsley, mint and thyme). For this purpose, liquid chromatography mass spectrometry (LC-MS) was used for the complete profiling of polyphenolic compounds and quantification of abundant phenolic compounds was completed with high-performance liquid chromatography—photodiode array detection (HPLC-PDA). Polyphenols from Australian-grown herbs were estimated through total phenolic content (TP), total flavonoids (TF) and total tannins (TT) along with their in-vitro antioxidant activities. Oregano and mint were estimated with the highest value of TP (140.59 ± 9.52 and 103.28 ± 8.08 mg GAE/g, milligram gallic acid equivalent/gram) while rosemary and mint had the highest TF (8.19 ± 0.74 and 7.05 ± 0.43 mg QE (quercetin equivalent)/g). In this study, eighty-four (84) phenolic compounds were screened and confirmed through LC-MS/MS by comparing their masses and fragmentation pattern with published libraries. The results of this study validate the use of these herbs as bioactives and their positive impact on human health.

scholarly journals HPLC-DAD-ESI-MSn identification of phenolic compounds in cultivated strawberries from Macedonia

2010 ◽  
Vol 29 (2) ◽  
pp. 181 ◽  
Author(s):  
Marina Kajdžanoska ◽  
Viktor Gjamovski ◽  
Marina Stefova
Keyword(s):  
Phenolic Compounds ◽  
Ellagic Acid ◽  
High Performance ◽  
Negative Ion ◽  
Ionization Mass ◽  
Photodiode Array Detection ◽  
Photodiode Array ◽  
Array Detection ◽  
Strawberry Cultivar ◽  
Negative Ion Mode

Strawberry fruits contain phenolic compounds that exhibit antioxidant, anticancer, antiatherosclerotic, antinflammatory and anti-neurodegenerative properties. High-performance liquid chromatography (HPLC) coupled to electrospray ionization mass spectrometric (ESI-MS) detection in the positive and negative ion mode has been used to identify the phenolic compounds in extracts from sixteen different strawberry cultivar fruits from Republic of Macedonia. Photodiode-array detection (DAD) has been used for screening of the different classes of phenolic compounds, whereas MS and MSn fragmentation data were employed for their structural characterization. The phenolic compounds identified were grouped as: ellagic acid and ellagic acid conjugates with sugars, ellagitannins, anthocyanins, flavonols, flavanols, and acylated sugars (feruloyl, caffeoyl and coumaroyl hexoses). Quercetin and kaempferol were the major flavonols found as quercetin 3-O-glucoside, quercetin 3-O-glucuronide, kaempferol 3-O-glucoside, kaempferol 3-O-glucoronide, kaempferol 3-O-malonylglucoside, kaempferol 3-O-acetylglucoside and kaempferol 3-O-coumaroylglucoside. Pelargonidin-3-O-glucoside was the most abundant anthocyanin in all strawberry extracts. Proanthocyanidins were also identified by MSn fragmentation as dimers, trimers and tetramers of (epi)catechin and (epi)afzelechin. This is the first assay of the phenolic profile of the strawberry cultivars in Macedonia, which can be further developed for characterization and evaluation of their quality with regards to their phenolic composition.

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