scholarly journals Influence of Different Solvents on the Phenolic Compounds Content Grape Bark Extracts (Vitis vinifera) from Syrah Variety

2020 ◽  
Vol 2 (3) ◽  
pp. 105-110
Author(s):  
Euzélia Lima de Souza1 ◽  
Talita Sousa Nascimento ◽  
Gabriele De Abreu Barreto ◽  
Ingrid Lessa Leal ◽  
Jeancarlo Pereira dos Anjos ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Phenolic Compounds ◽  
Vitis Vinifera ◽  
High Performance ◽  
Wine Industry ◽  
Standard Curve ◽  
Grain Alcohol ◽  
Grape Peel

Grape bark from the wine industry still contains significant amounts of phenolic compounds. Specific characteristics of the applied solvents influence the extraction of phenolic compounds directly. This study aimed to evaluate the influence of different solvents obtaining phenolic compounds in Syrah grape barkextracts by High-Performance Liquid Chromatography (HPLC). Grape peel extracts were prepared with three different solvents (water, 50%, and 80% grain alcohol), and evaluated using standard curve with 15 phenolic compounds. It was not possible to quantify the phenolic compounds, but the chromatogramssuggest the presence of some of them. The solvent that provided the best interaction with grape peel was 50% grain alcohol.

scholarly journals Seasonal Variation of Health-Promoting Bioactives in Broccoli and Methyl-Jasmonate Pre-Harvest Treatments to Enhance Their Contents

Foods ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 1371 ◽  
Author(s):  
Vanesa Nuñez-Gómez ◽  
Nieves Baenas ◽  
Inma Navarro-González ◽  
Javier García-Alonso ◽  
Diego A. Moreno ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Phenolic Compounds ◽  
Methyl Jasmonate ◽  
Bioactive Compounds ◽  
High Performance ◽  
Total Phenolic ◽  
Diode Array ◽  
Total Carotenoids ◽  
Health Promoting

Broccoli is a source of bioactive compounds that provide an important nutritional value. The content of these compounds can vary depending on agronomic and environmental conditions, as well as on elicitation. In this study, three crop trials were carried out to evaluate the effects of the cultivation season, the application of different dosages of methyl-jasmonate (MeJA) on the overall quality and on the total content of bioactive compounds of ‘Parthenon’ broccoli cultivated under the field conditions of southeastern Spain. Color parameters, chlorophyll content, total phenolic compounds, total flavonoids and antioxidant activity were measured to evaluate the overall quality. Moreover, individual carotenoids, phenolic compounds and glucosinolates were evaluated by high performance liquid chromatography with diode array detection (HPLC-DAD) and high performance liquid chromatography equipped with diode array detector coupled to mass spectrometer using electro spray ionization (HPLC-DAD-ESI/MSn). The content of total carotenoids, phenolic compounds and glucosinolates were higher in autumn compared with spring, showing increases of 2.8-fold, 2-fold and 1.2-fold, respectively. Moreover, a double application of MeJA increased the contents of total carotenoids, phenolic compounds and glucosinolates by 22%, 32% and 39%, respectively, relative to the untreated samples. Considering our results, the controlled and timely application of 250 µM MeJA to the aerial parts of the plants four days before harvest, on two consecutive days, seems to be a valid agronomic strategy to improve the health-promoting capacity of Parthenon broccoli, without compromising its overall quality.

Sign in / Sign up

Export Citation Format

Share Document