scholarly journals Size and Shape-Dependent Antimicrobial Activities of Silver and Gold Nanoparticles: A Model Study as Potential Fungicides

Molecules ◽  
2020 ◽  
Vol 25 (11) ◽  
pp. 2682 ◽  
Author(s):  
Francis J. Osonga ◽  
Ali Akgul ◽  
Idris Yazgan ◽  
Ayfer Akgul ◽  
Gaddi B. Eshun ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Pathogenic Fungi ◽  
Antimicrobial Activities ◽  
Plant Diseases ◽  
Production Plant ◽  
Silver And Gold Nanoparticles ◽  
Size And Shape ◽  
Model Study ◽  
Pathogenic Microbes ◽  
Bacteria And Fungi

Plant-based pathogenic microbes hinder the yield and quality of food production. Plant diseases have caused an increase in food costs due to crop destruction. There is a need to develop novel methods that can target and mitigate pathogenic microbes. This study focuses on investigating the effects of luteolin tetraphosphate derived silver nanoparticles (LTP-AgNPs) and gold nanoparticles (LTP-AuNPs) as a therapeutic agent on the growth and expression of plant-based bacteria and fungi. In this study, the silver and gold nanoparticles were synthesized at room temperature using luteolin tetraphosphate (LTP) as the reducing and capping agents. The synthesis of LTP-AgNPs and LTP-AuNP was characterized by Transmission Electron Microscopy (TEM) and size distribution. The TEM images of both LTP-AgNPs and LTP-AuNPs showed different sizes and shapes (spherical, quasi-spherical, and cuboidal). The antimicrobial test was conducted using fungi: Aspergillus nidulans, Trichaptum biforme, Penicillium italicum, Fusarium oxysporum, and Colletotrichum gloeosporioides, while the class of bacteria employed include Pseudomonas aeruginosa, Aeromonas hydrophila, Escherichia coli, and Citrobacter freundii as Gram (−) bacteria, and Listeria monocytogenes and Staphylococcus epidermidis as Gram (+) bacterium. The antifungal study demonstrated the selective size and shape-dependent capabilities in which smaller sized spherical (9 nm) and quasi-spherical (21 nm) AgNPs exhibited 100% inhibition of the tested fungi and bacteria. The LTP-AgNPs exhibited a higher antimicrobial activity than LTP-AuNPs. We have demonstrated that smaller sized AgNPs showed excellent inhibition of A. nidulans growth compared to the larger size nanoparticles. These results suggest that LTP-AuNP and LTP-AgNPs could be used to address the detection and remediation of pathogenic fungi, respectively.

scholarly journals Multi-Faceted Role of Silver and Gold Nanoparticles Synthesized from Biowaste and its in vitroAntibacterial, Antifungal and Antidiabetic Activities

2021 ◽  
Vol 11 (1) ◽  
pp. 3076-3092
Keyword(s):  
Gold Nanoparticles ◽  
Open Water ◽  
Antimicrobial Activities ◽  
Assay Method ◽  
Innovative Strategy ◽  
Silver And Gold Nanoparticles ◽  
Resazurin Assay ◽  
Ic50 Values ◽  
Uv Visible

Increasing global fish production, increasing the amount of waste generated, unsafe disposal of waste tissues like head, bones, skin, scales, fins etc., into land and open water reservoirs leads to environmental pollution. The role of nanobiotechnology in biowaste management is an innovative strategy to handle environmental issues. This study synthesized silver and gold nanoparticles from prawn heads using one millimolar AgNO3 and HAuCl4. Biosynthesized nanoparticles were characterized by UV -Visible spectroscopy, XRD, FTIR, SEM and EDAX. The maximum absorption spectrum was monitored at 437 nm for silver and 552 for gold nanoparticles. Antimicrobial activity was assessed using the resazurin assay method. MIC values obtained for the tested organisms revealed antimicrobial activities. P aeruginosa, K. pneumoniae, showed MIC at 15.6 µg for silver nanoparticles, and A. niger, A.flavus and C.albicans showed MIC at 125 µg for gold nanoparticles synthesized from the prawn head extract. IC 50 values of α- amylase activity were found to be 296 and 356 µg/ml for the silver and gold nanoparticles, respectively. IC50 values are about 705 and 2475 µg/ml for the silver and gold nanoparticles, respectively, in α-glucosidase activity. Conclusively silver and gold nanoparticles synthesized from prawn head extract (PHE) showed antibacterial, antifungal, and antidiabetic activities.

scholarly journals Plasmon-Modulated Excitation-Dependent Fluorescence and Antimicrobial Activities of Silver and Gold Nanoparticles Prepared with Eugenia unifloraL. extracts

2021 ◽  
Author(s):  
Marcia Regina Franzolin ◽  
Daniella dos Santos Courrol ◽  
Karina de oliveira Gonçalves ◽  
Lilia Coronato Courrol
Keyword(s):  
Gold Nanoparticles ◽  
Field Enhancement ◽  
Ph Control ◽  
Antimicrobial Activities ◽  
Excitation Wavelength ◽  
Xenon Lamp ◽  
Local Field Enhancement ◽  
Silver And Gold Nanoparticles ◽  
Gram Positive Bacteria ◽  
Nanoparticles Stability

Abstract Green synthesis using plant extract is a sustainable method to obtain silver and gold nanoparticles (Ag and AuNPs) and was employed in this work. The Eugenia uniflora L. fruits and leaves extracts were used in nanoparticles synthesis. The photoreduction process with a xenon lamp and pH control improved optical properties and nanoparticles stability. The UV-vis, TEM, FTIR, and Zeta potential of the prepared solutions were obtained. The fluorescence spectra of Ag and AuNPs were investigated at different excitation wavelengths, which showed two kinds of fluorescence peaks. The shorter wavelength peaks red-shift with the increasing excitation wavelength, which results from the electron interband transitions, and the longer fixed wavelength peaks due to the local field enhancement. Finally, the antimicrobial tests were performed with Gram-negative and Gram-positive bacteria and Candida albicans. The best results were obtained with EuAgNPs prepared with fruits extract, photoreduction, and pH 7.0 (with a mean of 95.12% ± 10.29% of inhibition).

scholarly journals Impact of Erodium moschatum (L.) L.'Hér. extracts against some bacteria and fungi species

2018 ◽  
Vol 7 (11) ◽  
pp. 2457
Author(s):  
Mostafa Qahtan Mosataf ◽  
Sirwan Hassan Salih ◽  
Talib Ouaid Alkhazraji
Keyword(s):  
Pathogenic Bacteria ◽  
Pathogenic Fungi ◽  
Trichophyton Mentagrophytes ◽  
Antimicrobial Activities ◽  
Plant Parts ◽  
Streptococcus Lactis ◽  
Entire Plant ◽  
The Difference ◽  
Bacteria And Fungi ◽  
Geographical Locations

The effectiveness of Erodium moschatum extracts (ethanol and methanol, water) were tested against pathogenic bacteria (two gram positive: Staphylococcus aureus and Streptococcus lactis and two gram negative: Escherichia coli and Proteus mirabilis) and four pathogenic fungi (Microsprum canis, Trichophyton mentagrophytes, Pencillium chrysogenum and Fusarium oxysporeum). Tannins, flavonoid and glycoside contents were studied by using HPLC technique and their antimicrobial activities were examined. The results showed the extracts efficacy on the tested microbes in which the ethanol with 11.11mm methanol with 11.22mm diameter were the most effective against the studied bacteria compared with the rest of the studied extracts, either in the fungi it was found that the studied extracts did not have significant differences in their effectiveness against the tested fungi, the results seemed that there are a difference in the concentration of studied active compounds according to the environment (geographical locations) and the difference in the plant parts, rutin showed the highest concentration in the entire plant (Sulaymaniyah) at concentration 22.92ppm compared to the rest of the compounds and Saladin locality and the studied plant part.

Antibacterial and Anti-Biofilm Biosynthesised Silver and Gold Nanoparticles for Medical Applications: Mechanism of Action, Toxicity and Current Status

2020 ◽  
Vol 17 (2) ◽  
pp. 88-100 ◽  
Author(s):  
Sundos Suleman Ismail Abdalla ◽  
Haliza Katas ◽  
Fazren Azmi ◽  
Mohd Fauzi Mh Busra
Keyword(s):  
Gold Nanoparticles ◽  
Synthesis Method ◽  
Chemical Characterization ◽  
Current Status ◽  
Silver And Gold Nanoparticles ◽  
Physical And Chemical Characterization ◽  
Biosynthesized Agnps ◽  
Physical And Chemical ◽  
Insight Into ◽  
Bio Synthesis

Fast progress in nanoscience and nanotechnology has contributed to the way in which people diagnose, combat, and overcome various diseases differently from the conventional methods. Metal nanoparticles, mainly silver and gold nanoparticles (AgNPs and AuNPs, respectively), are currently developed for many applications in the medical and pharmaceutical area including as antibacterial, antibiofilm as well as anti-leshmanial agents, drug delivery systems, diagnostics tools, as well as being included in personal care products and cosmetics. In this review, the preparation of AgNPs and AuNPs using different methods is discussed, particularly the green or bio- synthesis method as well as common methods used for their physical and chemical characterization. In addition, the mechanisms of the antimicrobial and anti-biofilm activity of AgNPs and AuNPs are discussed, along with the toxicity of both nanoparticles. The review will provide insight into the potential of biosynthesized AgNPs and AuNPs as antimicrobial nanomaterial agents for future use.

Design and synthesis of new antifungals based on N- un-substituted azoles as 14α demethylase inhibitor

2020 ◽  
Vol 16 ◽  
Author(s):  
Asghar Davood ◽  
Aneseh Rahimi ◽  
Maryam Iman ◽  
Parisa Azerang ◽  
Soroush Sardari ◽  
...  
Keyword(s):  
Antimicrobial Agents ◽  
Antifungal Agents ◽  
Molecular Design ◽  
Pathogenic Fungi ◽  
Antimicrobial Activities ◽  
Docking Studies ◽  
Receptor Interaction ◽  
Main Role ◽  
Design And Synthesis ◽  
Antifungal Antibiotics

Objective(s): Azole antifungal agents, which are widely used as antifungal antibiotics, inhibit cytochrome P450 sterol 14α-demethylase (CYP51). Nearly all azole antifungal agents are N-substituted azoles. In addition, an azolylphenalkyl pharmacophore is uniquely shared by all azole antifungals. Due to importance of nitrogen atom of azoles (N-3 of imidazole and N-4 of triazole) in coordination with heme in the binding site of the enzyme, here a group of N- un-substituted azoles in which both of nitrogen is un-substituted was reported. Materials and Methods: Designed compounds were synthesized by reaction of imidazole-4-carboxaldehyde with appropriate arylamines and subsequently reduced to desired amine derivatives. Antifungal activity against Candida albicans and Saccharomyces cervisiae were done using a broth micro-dilution assay. Docking studies were done using AutoDock. Results: Antimicrobial evaluation revealed that some of these compounds exhibited moderate antimicrobial activities against tested pathogenic fungi, wherein compound 3, 7 and 8 were potent. Docking studies propose that all of the prepared azoles interacted with 14α-DM, wherein azole-heme coordination play main role in drug-receptor interaction. Conclusion: Our results offer some useful references in order to molecular design performance or modification of this series of compounds as a lead compound to discover new and potent antimicrobial agents.

Stabilization of Silver and Gold Nanoparticles: Preservation and Improvement of Plasmonic Functionalities

2018 ◽  
Vol 119 (1) ◽  
pp. 664-699 ◽  
Author(s):  
Hyunho Kang ◽  
Joseph T. Buchman ◽  
Rebeca S. Rodriguez ◽  
Hattie L. Ring ◽  
Jiayi He ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Silver And Gold Nanoparticles

scholarly journals The Use of Essential Oils from Thyme, Sage and Peppermint against Colletotrichum acutatum

Plants ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 114
Author(s):  
Armina Morkeliūnė ◽  
Neringa Rasiukevičiūtė ◽  
Lina Šernaitė ◽  
Alma Valiuškaitė
Keyword(s):  
Essential Oils ◽  
Fungal Pathogens ◽  
Control Plant ◽  
Antimicrobial Activities ◽  
Plant Diseases ◽  
Colletotrichum Acutatum ◽  
Low Toxicity ◽  
Colletotrichum Spp ◽  
Biological Protection

The Colletotrichum spp. is a significant strawberry pathogen causing yield losses of up to 50%. The most common method to control plant diseases is through the use of chemical fungicides. The findings of plants antimicrobial activities, low toxicity, and biodegradability of essential oils (EO), make them suitable for biological protection against fungal pathogens. The aim is to evaluate the inhibition of Colletotrichum acutatum by thyme, sage, and peppermint EO in vitro on detached strawberry leaves and determine EO chemical composition. Our results revealed that the dominant compound of thyme was thymol 41.35%, peppermint: menthone 44.56%, sage: α,β-thujone 34.45%, and camphor: 20.46%. Thyme EO inhibited C. acutatum completely above 200 μL L−1 concentration in vitro. Peppermint and sage EO reduced mycelial growth of C. acutatum. In addition, in vitro, results are promising for biological control. The detached strawberry leaves experiments showed that disease reduction 4 days after inoculation was 15.8% at 1000 μL L−1 of peppermint EO and 5.3% at 800 μL L−1 of thyme compared with control. Our findings could potentially help to manage C. acutatum; however, the detached strawberry leaves assay showed that EO efficacy was relatively low on tested concentrations and should be increased.

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