scholarly journals Cerebrosides and Steroids from the Edible Mushroom Meripilus giganteus with Antioxidant Potential

Molecules ◽  
2020 ◽  
Vol 25 (6) ◽  
pp. 1395
Author(s):  
András Sárközy ◽  
Zoltán Béni ◽  
Miklós Dékány ◽  
Zoltán Péter Zomborszki ◽  
Kinga Rudolf ◽  
...  
Keyword(s):  
Spectroscopic Analysis ◽  
Methanol Extract ◽  
Octadecadienoic Acid ◽  
Edible Mushroom ◽  
Fungal Metabolites ◽  
Balanced Diet ◽  
Antioxidant Effects ◽  
Bioactivity Profile ◽  
Notable Addition ◽  
Detailed Chemical

The detailed chemical analysis of the methanol extract of Meripilus giganteus (Pers.) P. Karst. led to the isolation of two new cerebrosides, mericeramides A (1) and B (2) together with cerebroside B (3), ergosterol (4), 3β-hydroxyergosta-7,22-diene (5), cerevisterol (6), 3β-hydroxyergosta-6,8(14),22-triene (7), 3β-O-glucopyranosyl-5,8-epidioxyergosta-6,22-diene (8) and (11E,13E)-9,10-dihydroxy-11,13-octadecadienoic acid (9). The structures of the compounds were determined on the basis of NMR and MS spectroscopic analysis. Mericeramide A (1) is the first representative of halogenated natural cerebrosides. The isolated fungal metabolites 1–9 were evaluated for their antioxidant activity using the oxygen radical absorbance capacity (ORAC) assay. Compounds 2, 5 and 9 proved to possess considerable antioxidant effects, with 2.50 ± 0.29, 4.94 ± 0.37 and 4.27 ± 0.05 mmol TE/g values, respectively. The result obtained gives a notable addition to the chemical and bioactivity profile of M. giganteus, highlighting the possible contribution of this species to a versatile and balanced diet.

scholarly journals DETERMINATION OF THE ANTIOXIDANT ACTIVITY OF PRASMAN LEAF EXTRACTS (AYAPANA TRIPLINERVIS [VAHL]) AND THE TOTAL FLAVONOID AND PHENOL CONTENTS OF THE MOST ACTIVE EXTRACTS

2020 ◽  
pp. 107-111
Author(s):  
YUNITRIANTI ◽  
BERNA ELYA ◽  
ARIKADIA NOVIANI
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Methanol Extract ◽  
Total Flavonoid ◽  
Dpph Assay ◽  
Frap Assay ◽  
Methanol Extracts ◽  
Uv Visible ◽  
Antioxidant Effects

Objective: Prasman (Ayapana triplinervis [Vahl]) is a plant that can eliminate free radicals through its antioxidant effects. However, little research hasbeen conducted to explore the antioxidant activity of the plant.Methods: A. triplinervis (Vahl) leaves, which were determined by the Indonesian Institute of Sciences (LIPI) Bogor, were used in this study. Meanwhile,extraction was performed using n-hexane, ethyl acetate, and methanol as the solvents. Based on the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay usinga UV–visible spectrophotometer and based on ferric reducing antioxidant power (FRAP) assay using a microplate reader.Results: Based on the DPPH assay using a UV–visible spectrophotometer, n-hexane, ethyl acetate, and methanol extracts with a final concentrationof 25 μg/mL inhibited DPPH radical production by 38.91, 51.03, and 54.06%, respectively. Using the percent inhibition, the IC50 for the ethyl acetateand methanol extracts were 28.71 and 23.472 μg/mL, respectively. Based on FRAP assay using a microplate reader, the n-hexane, ethyl acetate, andmethanol extracts had ferrous equivalent antioxidant capacity values of 460, 828.99, and 940.22 μmol/g, respectively. The methanol extract had thegreatest antioxidant activity. The ethyl acetate and methanol extracts at the initial concentrations contained total phenol levels of 12.06 and 42.11 mgGAE/g extract, respectively, as well as total flavonoid levels of 3.24 and 3.41 mg QE/g extract, respectively.Conclusion: Based on the determination of phenol and flavonoid levels, the methanol extract had the greatest antioxidant effects.

scholarly journals Constituents of the Green Tea Seeds of Camellia sinensis

2011 ◽  
Vol 6 (3) ◽  
pp. 1934578X1100600
Author(s):  
Toshio Hasegawa ◽  
Kensuke Akutsu ◽  
Yasuhiro Kishi ◽  
Kouji Nakamura
Keyword(s):  
Mass Spectrometry ◽  
Fatty Acid ◽  
Nmr Spectroscopy ◽  
Green Tea ◽  
Camellia Sinensis ◽  
Spectroscopic Analysis ◽  
Methanol Extract ◽  
Tea Leaves ◽  
Hexane Extracts ◽  
Green Tea Leaves

Green tea ( Camellia sinensis) leaves are known to contain active ingredients such as catechins and caffeine, and are widely useful materials. Recently, green tea flowers also have been in the spotlight. However, little attention has been paid to the tea seeds. In this work, the constituents of green tea seeds and green tea leaves were compared. Caffeine was found in the seeds, whereas catechins (usually obtained from green tea leaves) were not observed. Next, we investigated the constituents of hexane extracts and methanol extracts of green tea seeds. We found that the hexane extracts contained high amounts of oleic glyceride (79.9%) in addition to linoleic glyceride (20%). We confirmed the structures of these glycerides by NMR spectroscopy and by synthesis from a fatty acid and glycerol. The methanol extract was found to contain naringenin glucosides by mass spectrometry and NMR spectroscopic analysis.

scholarly journals Chemical Constituents and Protection of Biodiversity of Corypha taliera Roxb., a Critically Endangered Plant of Bangladesh

2018 ◽  
Vol 20 (2) ◽  
pp. 213-220
Author(s):  
Akhtaruzzaman Chowdhury ◽  
Md Ashraful Alam ◽  
Md Shafiullah Shajib ◽  
Mohammad Abdullah Al Mansur ◽  
Mohammad A Rashid
Keyword(s):  
Spectroscopic Analysis ◽  
Methanol Extract ◽  
Chemical Constituents ◽  
Pharmaceutical Journal ◽  
Critically Endangered ◽  
Ex Situ ◽  
Endangered Plant ◽  
Separation And Purification ◽  
First Time ◽  
Mature Seeds

This article focuses on the chemical constituents and protection of biodiversity through plantation of saplings of Corypha taliera Roxb., a critically endangered plant of Bangladesh. Until 2010, the tree in the campus of University of Dhaka, used to be considered as the lone surviving species in the world in nature. Succesive chromatographic separation and purification of the methanol extract of air dried flowers of C. taliera provided β-sitosterol (1), β-amyrin (2), and betulinic acid (3) for the first time from its flowers. The structures of these purified compounds were established by extensive spectroscopic analysis and comparison of spectral data with published values as well as co-TLC with authentic samples. On the other hand, 500 mature seeds were sown in seed beds in the Medicinal Plant Garden of Faculty of Pharmacy, University of Dhaka, and Azimpur Government Officers' Quarter premises. After 40 days, the root was first seen to grow in its habitat and 85 days later the shoot developed up to 2.5 cm in height. The rate of germination was found to be 89-93%. The produced saplings were later on planted in different places of Bangladesh for conservation of the plant and protection of biodiversity by ex situ arrangement.Bangladesh Pharmaceutical Journal 20(2): 213-220, 2017

scholarly journals Chemical constituents and theira-glucosidase inhibitory activity of the stem of Salacia chinensis L.

2021 ◽  
Vol 5 (3) ◽  
pp. first
Author(s):  
Huu Tho Le ◽  
Truc Thanh Thi Duong ◽  
Phu Hoang Dang ◽  
Truong Nhat Van Do ◽  
Hai Xuan Nguyen ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Back Pain ◽  
Inhibitory Activity ◽  
Spectroscopic Analysis ◽  
Methanol Extract ◽  
Chemical Constituents ◽  
Positive Control ◽  
Ic50 Values ◽  
Treatment Of Diabetes ◽  
Chcl3 Fraction

Salacia chinensis L., known as “Chop mao” in Vietnam, is a climbing shrub that belongs to the Celastraceae family. The stem of S. chinensis L. is used as a traditional medicine for the treatment of diabetes, rheumatoid arthritis, back pain, … The dried powdered stem of S. chinensis L. was collected in Phu Yen province and was extracted with methanol to yield methanol extract. The methanol extract was suspended in H2O and partitioned successively with n-hexane, CHCl3, EtOAc to obtain n-hexane, CHCl3, EtOAc, and H2O fractions, respectively. The CHCl3 fraction was subjected to a series of chromatographic separation to afford four purified compounds including 3-oxolup-20(29)-en-30-al (1), betulin-3-caffeate (2), 2-(4-hydroxy-3,5-dimethoxylphenyl)ethanol (3), and acetosyringone (4). Their structures were elucidated on the basis of the spectroscopic analysis and comparison with literature data. The isolated compounds were tested for their α-glucosidase inhibitory activity. The result indicated that all compounds (1-4) possessed significant α-glucosidase at the testing concentration of 100 µM with the percent inhibition values of 9.5 ± 1.3, 70.89 ± 0.25, 44.2 ± 1.6, and 6.7 ± 1.7 %, respectively. In addition, betulin-3-caffeate (2) and 2-(4-hydroxy-3,5-dimethoxylphenyl)ethanol (3) show more potent α-glucosidase inhibitory activity, with IC50 values of 69.7 and 152.0 µM, respectively, than that of positive control acarbose (IC50 = 214.5 µM).

scholarly journals Compounds with Antifouling Activities from the Roots of Notopterygium franchetii

2015 ◽  
Vol 10 (12) ◽  
pp. 1934578X1501001
Author(s):  
Chun Yu ◽  
Liqing Cheng ◽  
Zhongling Zhang ◽  
Yu Zhang ◽  
Chunmao Yuan ◽  
...  
Keyword(s):  
Spectroscopic Analysis ◽  
Methanol Extract ◽  
Larval Settlement ◽  
Bugula Neritina ◽  
Antifouling Activity

In antifouling screening, the extract of Notopterygium franchetii de Boiss showed obvious activity. Two new phenylpropanoids (1–2) and five known coumarins (3-7) were isolated from the methanol extract of the roots of this species. The structures of the isolated compounds were determined on the basis of spectroscopic analysis. Compounds 1–2 showed definite antifouling activity against larval settlement of Bugula neritina.

scholarly journals Bioactive Metabolites from the Deep-Sea-Derived Fungus Diaporthe longicolla FS429

Marine Drugs ◽  
2020 ◽  
Vol 18 (8) ◽  
pp. 381
Author(s):  
Zhaoming Liu ◽  
Yuchan Chen ◽  
Saini Li ◽  
Qinglin Wang ◽  
Caiyun Hu ◽  
...  
Keyword(s):  
Deep Sea ◽  
Spectroscopic Analysis ◽  
Methanol Extract ◽  
Tyrosine Phosphatase ◽  
Bioactive Metabolites ◽  
X Ray Diffraction ◽  
Antiproliferative Effects ◽  
X Ray ◽  
Ic50 Value ◽  
First Time

The chemical investigation of a methanol extract of the deep-sea-derived fungus Diaporthe longicolla FS429 led to the isolation of two novel diterpenoids longidiacids A and B (1 and 2), two new polyketides (3 and 4), two new cytochalasin analogues longichalasins A and B (6 and 8) and three known analogues 5, 7, 9. Their structures were elucidated through comprehensive spectroscopic analysis, while the absolute configurations were established by the comparison of the experimental and quantum chemical calculated ECD spectra. The structure of compound 7 was confirmed through X-ray diffraction for the first time. In the bioassays compound 8 exhibited antiproliferative effects against SF-268, with an IC50 value of 16.44 μM. Moreover, compounds 1 and 8 were detected to inhibit 35.4% and 53.5% of enzyme activity of Mycobacterium tuberculosis protein tyrosine phosphatase B (MptpB) at a concentration of 50 μM.

Antioxidant and Hepatoprotective Effects ofAnoectochilus formosanusandGynostemma pentaphyllum

2000 ◽  
Vol 28 (01) ◽  
pp. 87-96 ◽  
Author(s):  
Chun-Ching Lin ◽  
Pei-Chen Huang ◽  
Jer-Min Lin
Keyword(s):  
Liver Injury ◽  
Hepatic Injury ◽  
Methanol Extract ◽  
Antiinflammatory Activity ◽  
Water Extract ◽  
Hepatoprotective Activity ◽  
Central Vein ◽  
Crude Drugs ◽  
Anoectochilus Formosanus ◽  
Antioxidant Effects

Anoectochilus formosanus Hay. and Gynostemma pentaphyllum Makino are popular folk medicines that have been used for treating hepatitis, hypertension and cancer in Taiwan. Our previous studies showed that these crude drugs exert antiinflammatory activity and hepatoprotective activity against CCI4-induced liver damage. In this study, the antioxidant effect of these crude drugs and their hepatoprotective activity on acetaminophen-induced liver injury in rat was evaluated. Our results suggest that A. formosanus and G. pentaphyllum do have antioxidant effects. On acetaminophen-intoxicated model, the increased levels of aspartate aminotransferase (AST) and alanine aminotrans-ferase (ALT) by acetaminophen administration were reduced by treatment with these two herbs. In histological observation, gross necrosis in the centribular area, sinusoidal congestion, infiltration of the lymphocytes and Kupffer cells around the hepatic central vein, and loss of cell boundaries and ballooning degeneration were reduced with herbal treatment. However, the effect of A. formosanus and G. pentaphyllum is biphasic. Methanol extract (100 and 300 mg/kg) and water extract (300 and 500 mg/kg) of A formosanus and water extract (100, 300 and 500 mg/kg) of G. pentaphyllum enhanced the recovery of liver injury while treatment with 500 mg/kg of A. formosanus methanol extract resulted in serious hepatic injury.

scholarly journals A New Compound from the Rhizomes of Boesenbergia pandurata

2018 ◽  
Vol 13 (6) ◽  
pp. 1934578X1801300
Author(s):  
Truong Nhat Van Do ◽  
Hai Xuan Nguyen ◽  
Tho Huu Le ◽  
Trang My Thi Ngo ◽  
Phu Hoang Dang ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Secondary Metabolite ◽  
Cell Lines ◽  
Cancer Cell ◽  
Spectroscopic Analysis ◽  
Pancreatic Cancer Cell ◽  
Methanol Extract ◽  
Human Pancreatic Cancer ◽  
Human Pancreatic Cancer Cell ◽  
Phytochemical Investigation

Phytochemical investigation of the methanol extract of the rhizomes of Vietnamese Boesenbergia pandurata resulted in the isolation of a new secondary metabolite named panduratin P (1). The structure of this compound was elucidated by NMR spectroscopic analysis. Compound 1 exhibited a mild activity against PANC-1 human pancreatic cancer cell lines in nutrient-deprived medium (NDM) with PC50 value of 55.9 μM.

scholarly journals Novel Antifungal Dimers from the Roots of Taiwania cryptomerioides

Molecules ◽  
2022 ◽  
Vol 27 (2) ◽  
pp. 437
Author(s):  
Ming-Jen Cheng ◽  
Ming-Der Wu ◽  
Chao-Lin Chang ◽  
Hsun-Shuo Chang ◽  
Chiou-Fung Chyu ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Candida Albicans ◽  
Aspergillus Niger ◽  
Spectroscopic Analysis ◽  
Methanol Extract ◽  
Penicillium Italicum ◽  
Antifungal Activities ◽  
Nmr Data ◽  
Taiwania Cryptomerioides

Five new dimer compounds, namely Taiwaniacryptodimers A‒E (1–5), were isolated from the methanol extract of the roots of Taiwania cryptomerioides. Their structures were established by mean of spectroscopic analysis and comparison of NMR data with those of known analogues. Their antifungal activities were also evaluated. Our results indicated that metabolites 1, 2, 4, and 5 displayed moderate antifungal activities against Aspergillus niger, Penicillium italicum, Candida albicans, and Saccharomyces cerevisiae.

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