scholarly journals Metabolite Profiling by UPLC-MSE, NMR, and Antioxidant Properties of Amazonian Fruits: Mamey Apple (Mammea Americana), Camapu (Physalis Angulata), and Uxi (Endopleura Uchi)

Molecules ◽  
2020 ◽  
Vol 25 (2) ◽  
pp. 342 ◽  
Author(s):  
Larissa Gabrielly Barbosa Lima ◽  
Julia Montenegro ◽  
Joel Pimentel de Abreu ◽  
Millena Cristina Barros Santos ◽  
Talita Pimenta do Nascimento ◽  
...  
Keyword(s):  
Bioactive Compounds ◽  
Metabolite Profiling ◽  
High Resolution Mass Spectrometry ◽  
Antioxidant Properties ◽  
Bioactive Compound ◽  
Ultra Performance Liquid Chromatography ◽  
Economic Exploitation ◽  
Ethanolic Extracts ◽  
Physalis Angulata ◽  
Amazonian Fruits

The metabolite profiling associated with the antioxidant potential of Amazonian fruits represents an important step to the bioactive compound′s characterization due to the large biodiversity in this region. The comprehensive bioactive compounds profile and antioxidant capacities of mamey apple (Mammea americana), camapu (Physalis angulata), and uxi (Endopleura uchi) was determined for the first time. Bioactive compounds were characterized by ultra-performance liquid chromatography coupled to high resolution mass spectrometry (UPLC-MSE) in aqueous and ethanolic extracts. Globally, a total of 293 metabolites were tentatively identified in mamey apple, campau, and uxi extracts. The main classes of compounds in the three species were terpenoids (61), phenolic acids (58), and flavonoids (53). Ethanolic extracts of fruits showed higher antioxidant activity and total ion abundance of bioactive compounds than aqueous. Uxi had the highest values of phenolic content (701.84 mg GAE/100 g), ABTS (1602.7 μmol Trolox g−1), and ORAC (15.04 μmol Trolox g−1). Mamey apple had the highest results for DPPH (1168.42 μmol TE g−1) and FRAP (1381.13 μmol FSE g−1). Nuclear magnetic resonance (NMR) spectroscopy results showed that sugars and lipids were the substances with the highest amounts in mamey apple and camapu. Data referring to chemical characteristics and antioxidant capacity of these fruits can contribute to their economic exploitation.

Bioactive compounds by UPLC-PDA in different cocoa clones (Theobroma cacao L.) developed in the Southern region of Bahia, Brazil

2017 ◽  
Vol 119 (9) ◽  
pp. 2117-2127 ◽  
Author(s):  
Leonardo Fonseca Maciel ◽  
Ana Lúcia de Souza Madureira Felício ◽  
Elisa Yoko Hirooka
Keyword(s):  
Bioactive Compounds ◽  
Phenolic Content ◽  
Principal Component ◽  
Bioactive Compound ◽  
Ultra Performance Liquid Chromatography ◽  
Southern Region ◽  
Cocoa Bean ◽  
Total Phenolic ◽  
Array Detector ◽  
Content Type

Purpose Many factors can influence the bioactive compounds in cocoa beans. The purpose of this paper is to characterize the bioactive compound profile of 12 cocoa bean clones produced in Bahia’s Southern region. Design/methodology/approach Total phenolic content, total flavonoids and total anthocyanins were determined using spectrophotometric methods. Caffeic acid, caffeine, theobromine, catechin and epicatechin contents were determined using Ultra Performance Liquid Chromatography with a Photodiode Array Detector. Principal component analysis (PCA) was carried out using to determine the variability of the results found in relation to the clones studied. Findings The authors observed variations in the bioactive compound content between the analyzed cocoa clones. The SRN clone showed the highest levels of phenolic content, flavonoids, caffeine, catechin, and epicatechin. PCA showed that all the clones had unique characteristics related to the composition of their bioactive compounds content, classifying each clone in a well-defined manner. Originality/value This work is an important contribution for the characterization of new cocoa bean clones in relation to their content of bioactive compounds. The information reported in this paper can be decisive in the choice of the variety of cocoa in the production of chocolate with high bioactive compounds content and claim of functional property.

Instant Insights: Nutraceuticals in fruit and vegetables

2020 ◽  
Keyword(s):  
Vitamin C ◽  
Bioactive Compounds ◽  
Crop Production ◽  
Metabolic Diseases ◽  
Antioxidant Properties ◽  
Bioactive Compound ◽  
Fruit And Vegetables ◽  
Specific Cell ◽  
Anthocyanin Content ◽  
Genetic Dissection

This specially curated collection features three reviews of current and key research on nutraceuticals in fruit and vegetables. The first chapter provides a brief description of the chemistry of bioactive compounds (BCs) and their presence in temperate fruits, and discusses recent advances in strategies towards improving sustainable crop production for nutraceuticals. It examines polyphenols, carotenoids, vitamin C and production practices that influence bioactive compound synthesis. The second chapter describes the claimed health benefits associated with the antioxidant properties of bioactive compounds found in mangoes, such as vitamin C, phenolics and carotenoids. The chapter also examines specific cell, animal and clinical studies that suggest mango pulp, juice and extract are effective against metabolic diseases and certain forms of cancer. The final chapter considers how developments such as genetic dissection using fruit ripening mutants, new transgenic plants, and molecular breeding have opened a road map for scientists to further unravel the intricacies and regulation of genes governing fruit quality attributes. Improvements in precision in engineering plant genomes have enabled development of novel tomatoes with marketable traits such as higher carotenoid and anthocyanin content, both beneficial for human health.

scholarly journals Determination of Phenolic Composition and Antioxidant Activity in Flowers and Stems of Rumex Tunetanus Using RP-UHPLC-ESI-QTOF-MS

2018 ◽  
Author(s):  
Jouda Abidi ◽  
Sonda Ammar ◽  
Mohamed Bouaziz
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Bioactive Compounds ◽  
Folk Medicine ◽  
Antioxidant Properties ◽  
Bioactive Compound ◽  
Selective Extraction ◽  
Total Phenolic ◽  
Negative Ionization Mode ◽  
Negative Ionization

The present study was designed to investigate the bioactive compound in Rumextunetanus extracts (polygonaceae), a plant growing in GarâaSejnane region (NW-Tunisia). Hydro-methanol extracts of flowers and stems of Rumextunetanus were analyzed by RP-UHPLC-ESI-QTOF-MS in the negative mode to identify the maximum of bioactive compounds. Applied the aforementioned method, a total of 60 bioactive compounds were characterized for the first time in Rumextunetanus between them, 18 photochemical were firstly identified in the Polygonaceae family in negative ionization mode. Quantification of the identified compounds revealed that quercetin-3-O-glucuronide and (-)-epicatechingallate were the most abundant phenolic compounds in flowers and stems, respectively. Moreover, positive correlations were found between the antioxidant activity measured by DPPH and FRAP assays with the total phenolic compounds (r = 0.98; r = 0.99, respectively) and the abundance of some phenolic subfamilies such as hydroxycinnamic acids, hydroxybenzoic acids, flavonols and flavones with r > 0.86. The compounds displaying significant (P < 0.01) and good correlations with the antioxidant activity (r > 0.93) were hydroxybenzoic acid, rutin, quercetin-3-O-glucuronide, quercetin-3-O-glucoside, quercetin and luteolin-7-O-rutinoside. In addition, the flowers and stems of Rumex tunetanus showed different bioactive compound profiles and significant antioxidant properties of extracts. These results highlight the potential of the RP-UHPLC-ESI-QTOF-MS and MS/MS system to identify untargeted metabolic profiling of Rumex tunetanus. Overall, these results contribute to the clear explanation of the past and current usage of genus Rumex in folk medicine. Future investigations are necessary to develop purified antioxidant extracts, with the application of more selective extraction techniques.

scholarly journals Characterisation and Anti-diabetic Activity of Phenylquinoline, and Narceine Isolated from Ficus polita Leaf

2020 ◽  
pp. 102-114
Author(s):  
A. Nasir ◽  
M. S. Sule ◽  
A. J. Alhassan ◽  
A. M. Wudil ◽  
A. A. Imam ◽  
...  
Keyword(s):  
Bioactive Compounds ◽  
Fasting Blood Glucose ◽  
Antioxidant Properties ◽  
Bioactive Compound ◽  
Diabetic Rats ◽  
Serum Total Bilirubin ◽  
Spectroscopic Techniques ◽  
Serum Total Protein ◽  
Pancreatic Toxicity ◽  
Antioxidant Markers

The present study was conducted to evaluate anti-diabetic potentials of column chromatography fractions (F1-F6) of chloroform leaf extract of Ficus polita and to detect the probable bioactive compounds present in the most active fraction using spectroscopic techniques. Antidiabetic potential of the fractions (F1-F6) were tested at a dose of 50 mg/kg on wistar rats. Fraction 3 and metformin treated diabetic groups showed significant decreases in fasting blood glucose (FBS) level, ameliorate hepatic and renal damages by decreasing the levels of serum total bilirubin, direct bilirubin AST, ALT, creatinine, urea, potassium and chloride, but increasing the levels of serum total protein, albumin, sodium, and bicarbonate compared to untreated diabetic rats. Fraction 3 also stimulates insulin secretion by β cells. After alloxan administration, the levels of hepatic and renal tissues antioxidant markers such as GSH, SOD and catalase were decreased whereas the level of hepatic and renal tissues MDA was elevated. The levels of these antioxidant markers were brought to normalcy by fraction 3 (F3). Histological studies of the pancreas supported the biochemical findings, and treatment with fraction 3 (F3) was found to be effective in restoring alloxan-induced pancreatic toxicity in rats. FTIR and GCMS and analyses were conducted for the detection of bioactive compound(s) in fraction 3 (F3) and the result revealed the presence of "8-methoxy-4-phenylquinoline and narceine The study concludes that; the anti-diabetic property of the leaf of Ficus polita is mediated by the bioactive compounds " 8-methoxy-4-phenylquinoline, and narceine." through their antioxidant properties and stimulation of damaged pancreas to produce more insulin.

scholarly journals The influence of weather conditions on bioactive compound content in sorghum grain

2019 ◽  
Vol 246 (1) ◽  
pp. 13-22 ◽  
Author(s):  
Anna Przybylska-Balcerek ◽  
Jakub Frankowski ◽  
Kinga Stuper-Szablewska
Keyword(s):  
Quantitative Analysis ◽  
Bioactive Compounds ◽  
Phenolic Acids ◽  
Bioactive Compound ◽  
Weather Conditions ◽  
Ultra Performance Liquid Chromatography ◽  
Temperate Climate ◽  
Total Carotenoids ◽  
Liquid Chromatograph ◽  
Sorghum Grain

Abstract Sorghum is the fifth most important cereal in the world in terms of the cropped area. It is mainly grown for feeding animals and it is also used in the food industry. Sorghum grain is generally a rich source of antioxidants such as polyphenols and carotenoids. For this reason, it is considered as a good source of bioactive food components and it has health-promoting properties. Sorghum is a gluten-free cereal grown in many regions worldwide, primarily in the tropical and subtropical regions. However, new hybrids and forms of sorghum are capable to produce seeds also in temperate climate. The aim of this study was to conduct the influence of weather conditions on bioactive compound content in sorghum grain. The quantitative analysis of selected bioactive compounds, such as phenolic acids, flavonoids, carotenoids, and phytosterols, was carried out. The tested material comprised grain of two varieties: ‘Sweet Susana’ and ‘Sweet Caroline’, which have different color of grain: red and white. The research material was obtained from growing seasons 2016–2018. Quantitative analysis of free phenolic acids, total carotenoids, and total phytosterols was performed by ultra-performance liquid chromatography (UPLC) after prior basic hydrolysis followed by acid. An ultra-efficient liquid chromatograph coupled with an absorption-based detector (UPLC-PDA) was used for these analyses. The results showed the variability of the content of bioactive compounds depending on weather conditions.

scholarly journals An Entire Process Optimization Strategy for Comprehensive In Vivo Metabolite Profiling of Prucalopride in Rats Based on Ultra-Performance Liquid Chromatography With Q-Exactive Hybrid Quadrupole–Orbitrap High-Resolution Mass Spectrometry

2021 ◽  
Vol 12 ◽  
Author(s):  
Lihua Zuo ◽  
Liwei Liu ◽  
Yantao Yang ◽  
Jie Yang ◽  
Min Chen ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
High Resolution ◽  
Oral Administration ◽  
Metabolite Profiling ◽  
High Resolution Mass Spectrometry ◽  
Ultra Performance Liquid Chromatography ◽  
Multiple Time ◽  
Q Exactive

Prucalopride was widely used for chronic constipation, which is difficult to be adequately relieved by laxatives in adult patients in clinic. Due to the difficulty in metabolite identification, metabolic process of prucalopride had not been investigated in vivo. In this study, an efficient strategy was proposed for comprehensive metabolite profiling of prucalopride after oral administration in rat plasma, urine, and feces samples. This strategy was composed of five steps. First, the samples at multiple time points after oral administration were collected to increase the representativeness of the samples. Second, different sample preparation methods were investigated to obtain superior extraction efficiency. Third, the raw data of test sample and blank sample were acquired using ultra-performance liquid chromatography with Q-Exactive hybrid quadrupole–orbitrap high-resolution accurate mass spectrometry under the positive and negative full-scan/dd MS2 mode. Fourth, combined mass defect filter with background subtraction model in soft of compound discovery, all peaks were constructed to filter potential metabolites after retention time alignment and ion filtration, which could remove large amounts of interference ions. Besides, it can predict potential biotransformation, promoting to understand how to metabolize the drug. This provides multiple possibilities and prevents us conjecturing the potential metabolites blindly. Finally, the verification procedure was implemented through exporting the structure and MS2 spectrum to the analytical tool of Mass Frontier. The proposed strategy significantly improved the targeted detection and identification for metabolites in vivo. A total of 47 metabolites were tentatively characterized in the plasma, urine, and feces samples after oral administration of prucalopride. This study could provide a valuable reference for systematic metabolite profile of drug in vivo.

scholarly journals Bioactive Compounds, Nutritional Traits, and Antioxidant Properties of Artocarpus altilis (Parkinson) Fruits: Exploiting a Potential Functional Food for Food Security on the Comoros Islands

2018 ◽  
Vol 2018 ◽  
pp. 1-11 ◽  
Author(s):  
Toilibou Soifoini ◽  
Dario Donno ◽  
Victor Jeannoda ◽  
Ernest Rakotoniaina ◽  
Soule Hamidou ◽  
...  
Keyword(s):  
Bioactive Compounds ◽  
Functional Food ◽  
Sugar Content ◽  
Antioxidant Properties ◽  
Bioactive Compound ◽  
Drying Process ◽  
Cinnamic Acids ◽  
Artocarpus Altilis ◽  
Lead Compounds ◽  
Phytochemical Investigation

Comoros Union presents a considerable biodiversity of food resources that are neglected or still not valorised, as breadfruit. This study aimed to evaluating nutritional and nutraceutical traits of Artocarpus altilis (Parkinson) Fosberg by characterizing its main bioactive compounds, nutritional traits, and antioxidant properties in order to contribute to the development of traditional and innovative uses of this species as functional food (e.g., infant flour). Bioactive compound composition, antioxidant properties, protein and sugar content, lipids, fibre, and macro- and microelements were observed in these fruits after a specific drying process. Breadfruit showed positive nutritional traits. The main identified phenolic groups were cinnamic acids (with a maximum of 51.88 ± 2.63 mg/100 gDW for chlorogenic acid) and tannins. The highest value of antioxidant activity was 6.40 ± 1.02 mmol·Fe2+/kgDW. This preliminary phytochemical investigation may provide a contribution to the identification and quantification of lead compounds responsible for traditional nutritional and therapeutic claims.

scholarly journals Effect of Solvents on Bioactive Compounds and Antioxidant Activity of Padina Tetrastromatica and Gracilaria Tenuistipitata Seaweeds Collected From Bangladesh

2021 ◽  
Author(s):  
Mohammad Khairul Alam Sobuj ◽  
Md. Ariful Islam ◽  
Md. Shoebul Islam ◽  
Md. Mohidul Islam ◽  
Yahia Mahmud ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Antioxidant Activity ◽  
Bioactive Compounds ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Reducing Power ◽  
Bioactive Compound ◽  
Total Phenolic ◽  
Gracilaria Tenuistipitata ◽  
Padina Tetrastromatica

Abstract Seaweeds are now recognized as a treasure of bioactive compounds. However, the seaweed of Bangladesh is still unexplored. So, this study was designed to explore the secondary metabolites and antioxidant activities of solvent extracts of Padina tetrastromatica and Gracilaria tenuistipitata. Phytochemical screening and Fourier transform infrared (FTIR) confirm the diverse type of bioactive compounds. Antioxidant activity of extracts were evaluated by 1, 1-diphenyl-2- picrylhydrazyl (DPPH), 2, 2-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), reducing power (RP), phosphomolybdenum and hydrogen peroxide assays. Here, methanolic extract of P. tetrastromatica showed the highest amount of total phenolic content (85.61 mg of GA/g), total flavonoid content (41.77 mg of quercetin/g), which showed as potent antioxidant properties confirmed by DPPH (77.07%), ABTS (77.65%), RP (53.24 mg AAE/g), phosphomolybdenum (31.58 mg AAE/g) and hydrogen peroxide (67.89%) assays followed by ethanol and water. This study concluded that bioactive compound and antioxidant activities are dose and solvent-dependent and seaweed type.

scholarly journals Ghanaian Cocoa (Theobroma cacao L.) Bean Shells Coproducts: Effect of Particle Size on Chemical Composition, Bioactive Compound Content and Antioxidant Activity

Agronomy ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 401
Author(s):  
Carmen Botella-Martínez ◽  
Raquel Lucas-Gonzalez ◽  
Carmen Ballester-Costa ◽  
José Ángel Pérez-Álvarez ◽  
Juana Fernández-López ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Bioactive Compounds ◽  
Theobroma Cacao ◽  
Antioxidant Properties ◽  
Bioactive Compound ◽  
Cocoa Bean ◽  
Particle Sizes ◽  
Physico Chemical ◽  
Polyphenolic Profile ◽  
Theobroma Cacao L

The worldwide cocoa bean shell (CBS) generation is estimated at around 900,000 tons. In their composition, this coproduct showed several bioactive compounds like methylxanthines or polyphenolic compounds. Thus, the aim of this work was to investigate the effects of different particle sizes on the chemical composition, physico-chemical, bioactive compounds content, and antioxidant properties of flours obtained from cocoa (Theobroma cacao L.) bean shells. The flours obtained from CBS with different particle sizes had high content of dietary fiber (61.18–65.58 g/100 g). The polyphenolic profile identified seven compounds being epicatechin and catechin (values ranged 4.56–6.33 and 2.11–4.56 mg/g, respectively) as the most abundant compounds. Additionally, the methylxanthines theobromine and caffeine were quantified with values ranging from 7.12 to 12.77 and 4.02 to 6.13 mg/g, respectively. For the fatty acid profile, the principal compounds identified were oleic, stearic and palmitic acids. CBS had antioxidant capacity with all methods assayed. For DPPH, ABTS and FRAP assays values ranged between 2.35–5.53, 3.39–11.55, and 3.84–7.62 mg Trolox equivalents/g sample, respectively. This study suggests that cocoa bean shells may constitute a valuable coproduct for the food industry due to its high content in valuable bioactive compounds.

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