scholarly journals Separation of Five Flavonoids from Aerial Parts of Salvia Miltiorrhiza Bunge Using HSCCC and Their Antioxidant Activities

Molecules ◽  
2019 ◽  
Vol 24 (19) ◽  
pp. 3448 ◽  
Author(s):  
Fan Yang ◽  
Yingxue Qi ◽  
Wei Liu ◽  
Jia Li ◽  
Daijie Wang ◽  
...  
Keyword(s):  
High Speed ◽  
Antioxidant Activities ◽  
Salvia Miltiorrhiza ◽  
Natural Antioxidants ◽  
2D Nmr ◽  
Solvent System ◽  
Positive Control ◽  
Salvia Miltiorrhiza Bunge ◽  
Two Phase ◽  
Aerial Parts

The aerial parts of Salvia miltiorrhiza Bunge, as the non-medicinal parts, are always discarded during harvesting, resulting in a huge waste of resources and environmental pressure. Due to the high flavonoid content and their antioxidant activities characteristics, the aerial parts of S. miltiorrhiza can be developed into natural antioxidants and used in foods. A high-speed counter-current chromatography (HSCCC) method, using a two-phase solvent system composed of tert-butyl methyl ether/n-butanol/acetonitrile/water (3:1:1:20, v/v), was the first to successfully isolate five flavonoids from the aerial parts of S. miltiorrhiza in one attempt, and separately categorized as rutin (1), isoquercitrin (2), kaempferol-3-O-α-l-rhamnopyranosyl-(1→6)-β-d-glucopyranoside (3), kaempferol-3-O-β-d-glucopyranoside (4) and apigenin-7-O-β-d-glucopyranoside (5) after identification. The purities of these plant isolates were 97.3%, 99.5%, 92.8%, 98.1% and 98.7%, respectively. All the flavonoids were identified by HR-ESI-MS, 1D and 2D NMR. Compounds 3 and 5 were firstly isolated from the plant of S. miltiorrhiza. Results from antioxidant assays showed that rutin (1) and isoquercitrin (2) had higher antioxidant capacities compared to L-ascorbic acid as the positive control.

scholarly journals Isolation and Purification of Galloyl, Caffeoyl, and Hexahydroxydiphenoyl Esters of Glucoses from Balanophora simaoensis by High-Speed Countercurrent Chromatography and Their Antioxidant Activities In Vitro

Molecules ◽  
2018 ◽  
Vol 23 (8) ◽  
pp. 2027 ◽  
Author(s):  
Lei Fang ◽  
Tian-Tian He ◽  
Xiao Wang ◽  
Jie Zhou
Keyword(s):  
High Speed ◽  
Antioxidant Activities ◽  
Solvent System ◽  
Ionization Mass ◽  
Isolation And Purification ◽  
Aerial Parts ◽  
Nuclear Magnetic Resonance Spectra ◽  
Ic50 Values ◽  
One Step

High-speed counter-current chromatography was used to separate and purify galloyl, caffeoyl, and hexahydroxydiphenoyl esters of glucoses from the aerial parts of the parasitic plant Balanophora simaoensis for the first time using n-hexane-ethyl acetate-methanol-water (1:2:1:2, v/v) as the optimum solvent system. Accordingly, 1-O-(E)-caffeoyl-3-O-galloyl-β-d-glucopyranose (I, 12.5 mg), 1-O-(E)-caffeoyl-3-O-galloyl-4,6-(S)-hexahydroxydiphenoyl-β-d-glucopyranose (II, 27.2 mg), and 1-O-(E)-caffeoyl-4,6-(S)-hexahydroxydiphenoyl-β-d-glucopyranose (III, 52.8 mg) with 98.0%, 98.5%, and 98.7% purities, respectively, were purified from 210 mg crude extract of B. simaoensis in a one-step separation. The structures of the glucose esters were identified by electrospray ionization mass spectrometry (ESI-MS) and nuclear magnetic resonance spectra (NMR). Their antioxidant activities were evaluated by measuring their inhibition activity on liver microsomal lipid peroxidation induced by the Fe2+-Cys system in vitro. Compounds I–III showed significant antioxidant activities with IC50 values ranging from 2.51 to 6.68 μm, respectively.

scholarly journals Separation of Five Iridoid Glycosides from Lonicerae Japonicae Flos Using High-Speed Counter-Current Chromatography and Their Anti-Inflammatory and Antibacterial Activities

Molecules ◽  
2019 ◽  
Vol 24 (1) ◽  
pp. 197 ◽  
Author(s):  
Ran Yang ◽  
Lei Fang ◽  
Jia Li ◽  
Zhenhua Zhao ◽  
Hua Zhang ◽  
...  
Keyword(s):  
High Speed ◽  
Iridoid Glycosides ◽  
Platelet Activating Factor ◽  
2D Nmr ◽  
Solvent System ◽  
Antibacterial Activities ◽  
Two Phase ◽  
Iridoid Glucosides ◽  
Anti Inflammatory ◽  
Counter Current

A high-speed counter-current chromatography (HSCCC) method, using a two-phase solvent system composed of ethyl acetate/n-butanol/methanol/water (5:1:1:5, v/v/v/v), was successfully established to separate the five iridoid glucosides 7-O-ethyl sweroside (1), secologanin dimethylacetal (2), adinoside F (3), (7R)-secologain n-butyl methyl acetal (4) and adinoside G (5) from Lonicerae Japonicae Flos. Their purities were 96.8%, 98.5%, 93.3%, 98.0% and 99.9%, respectively. All the iridoid glucosides were identified by HR-ESI-MS, 1D and 2D NMR. Compounds 3 and 5 are new iridoid glucosides. The anti-inflammatory tests showed that compounds 1–5 all expressed moderate inhibitory effects on β-glucuronidase release in rat polymorphonuclear leukocytes (PMNs) induced by platelet-activating factor (PAF) with IC50 values ranging from 4.52 to 6.50 µM, while the antibacterial assays demonstrated that all the compounds displayed mild inhibitory activities against Staphylococcus aureus ATCC 25923 with MIC values ranging from 13.7 to 26.0 µg/mL.

scholarly journals Two New Prenylated Isoflavonoids from Erinacea anthyllis with Antioxidant and Antibacterial Activities

2017 ◽  
Vol 12 (7) ◽  
pp. 1934578X1701200 ◽  
Author(s):  
Soumia Mouffouk ◽  
Laurence Marcourt ◽  
Mohammed Benkhaled ◽  
Kaouthar Boudiaf ◽  
Jean-Luc Wolfender ◽  
...  
Keyword(s):  
Antioxidant Activities ◽  
Optical Rotation ◽  
2D Nmr ◽  
Antibacterial Activities ◽  
Total Phenolic ◽  
Aerial Parts ◽  
Whole Plant ◽  
The Family ◽  
Chemotaxonomic Markers ◽  
Phenolic And Flavonoid

Two new prenylated isoflavonoids, namely Erinasone A and Erinasone B along with 19 known secondary metabolites, including twelve isoflavonoids, three polyphenols, one flavonol, two flavanones and three steroids, were isolated from the whole plant (roots and aerial parts) of Erinacea anthyllis. Structures of all isolated compounds were elucidated by spectroscopic analysis, including 1D and 2D NMR (1H, 13C, COSY, HSQC, TOCSY, HMBC and NOESY), mass spectrometry (ESI-MS), UV-Vis, measurement of optical rotation [α]D and by comparison with the literature data. The total phenolic and flavonoid contents were determined in this study. Furthermore, the antioxidant and antibacterial activities of the EtOAc and n-BuOH extracts of E. anthyllis were evaluated. These extracts exhibited moderate antibacterial and antioxidant activities. Their IC50 values were approximately 0.04 mg/mL ( n-BuOH) and 0.037 mg/mL (EtOAc). Consequently, Erinacea anthyllis is a rich source of polyphenolic compounds particularly isoflavonoids used as chemotaxonomic markers for the subfamily Papilionoideae of the family Fabaceae.

An Efficient Strategy Based on Liquid–Liquid Extraction With Acid Condition and HSCCC for Rapid Enrichment and Preparative Separation of Three Caffeoylquinic Acid Isomers From Mulberry Leaves

2019 ◽  
Vol 57 (8) ◽  
pp. 738-744 ◽  
Author(s):  
Li-Tao Wang ◽  
Ming-Zhu Gao ◽  
Qing Yang ◽  
Qi Cui ◽  
Yue Jian ◽  
...  
Keyword(s):  
High Speed ◽  
Solvent System ◽  
Macroporous Resin ◽  
Acid Condition ◽  
Two Phase ◽  
Caffeoylquinic Acids ◽  
Mulberry Leaves ◽  
Caffeoylquinic Acid ◽  
Liquid Liquid Extraction ◽  
Separation Strategy

Abstract Morus alba L. is a medicinal plant that contains a high amount of caffeoylquinic acids such as 3-caffeoylquinic acid (3-CQA), 5-caffeoylquinic acid (5-CQA) and 4-caffeoylquinic acid (4-CQA). This study aimed to establish a fast and efficient method for separating caffeoylquinic acids from mulberry leaves by using high-speed countercurrent chromatography coupled with macroporous resin. D101 resin showed better adsorption and desorption capacity for three caffeoylquinic acids among six macroporous resin adsorbents. The contents of 3-CQA, 5-CQA and 4-CQA reached for 4.77%, 18.95% and 9.84% through one cycle of D101 resin, which were 3.13-fold, 4.57-fold and 4.78-fold more than those in crude extracts, respectively. With a two-phase solvent system of ethyl acetate-water (1:1, V/V), >93% purity of target compounds were obtained in one cycle during 150 min with the recovery yields of 80.59%, 99.56% and 94.21% for 3-CQA, 5-CQA and 4-CQA, respectively. The structural identification of target compounds was carried out by ESI-MS, 1H NMR and 13C NMR spectra. The present result represented an easy and efficient separation strategy for the utilization of mulberry resource.

scholarly journals Quality Control of Psoralea corylifolia L. Based on High-Speed Countercurrent Chromatographic Fingerprinting

Molecules ◽  
2020 ◽  
Vol 25 (2) ◽  
pp. 279
Author(s):  
Xiaoxue Wu ◽  
Xuemin Gao ◽  
Xinmei Liu ◽  
Shuyi Zhang ◽  
Huayu Yang ◽  
...  
Keyword(s):  
Quality Control ◽  
High Speed ◽  
Solvent System ◽  
Chemical Components ◽  
Therapeutic Effects ◽  
Psoralea Corylifolia ◽  
Good Separation ◽  
Two Phase ◽  
Plant Materials

Traditional Chinese medicine (TCM)has played an important role in promoting the health of Chinese people. The TCM Psoralea corylifolia L. has been used in the treatment of various kinds of diseases including enuresis, vitiligo, and calvities. However, therapeutic effects of P. corylifolia L. have often influenced by the quality of plants. So, it is very important to control the quality of P. corylifolia L. In this study, analytical high-speed countercurrent chromatography (HSCCC) was successfully used to fingerprint P. corylifolia L. Samples of P. corylifolia L. were extracted by ultrasonic extraction. n-hexane-ethyl acetate–methanol–water at a ratio of 5:5.5:6.5:5 (v/v) was selected as a two-phase solvent system and the condition of HSCCC were optimized in order to good separation. And the method of HSCCC was verified (reproducibility, precision, and stability). HSCCC chromatograms exhibited six common peaks, which were selected as indicator compounds for the quality control of P. corylifolia L. Within 20 types of medicinal materials, chemical components are similar, but the levels of components are quite different in HSCCC fingerprint. The present results demonstrate that the HSCCC method provides a reliable basis for the quality control of P. corylifolia L. and can also be applied to confirm the authenticity of plant materials.

A Novel Methodology that Combined Fast Chromatography and Antioxidant Activities Screening in Baimai Prescription Research

2013 ◽  
Vol 699 ◽  
pp. 349-353
Author(s):  
Zi Qian Zhang ◽  
Xiao Yu Chen ◽  
Yun Xia Duan ◽  
Fang Liang ◽  
Qing Shan Liu ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
High Speed ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Absorption Spectrometry ◽  
Gradient Elution ◽  
Solvent System ◽  
Difficult Problem ◽  
Flash Chromatography

To tackle the difficult problem of large ethnomedicine compound prescription research in a fast and efficient way, high-speed separation of Baimai prescriptions was performed by automated flash chromatography on 100-g Si flash columns at a flow-rate of 40 mL/min. Petroleum ether, methylene dichloride, ethyl acetate, methanol and water were used in gradient elution solvent system. 69 fractions were obtained as an ethnodrug compounds library. Separation degree of partial fractions were determined by HPLC-UV absorption spectrometry. Antioxidant activities screening of the fractions in 96 well plates was carryied out. Distribution range of fractions with strong scavenging effect of free radical DPPH was identified. Fraction 5-18 and 20 extracted from ethyl acetate exert the strongest antioxidant activities in vitro, and are identified as effective-composite of the prescription in radical scavenging. The results reported here suggest that the methods used may lead to improvements in the research and development of large ethnomedicine compound prescription.

Separation of tanshinones from Salvia miltiorrhiza Bunge by high-speed counter-current chromatography using stepwise elution

2000 ◽  
Vol 904 (1) ◽  
pp. 107-111 ◽  
Author(s):  
Guilian Tian ◽  
Yabin Zhang ◽  
Tianyou Zhang ◽  
Fuquan Yang ◽  
Yoichiro Ito
Keyword(s):  
High Speed ◽  
Salvia Miltiorrhiza ◽  
Salvia Miltiorrhiza Bunge ◽  
Counter Current
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