Direct purification of tanshinones from Salvia miltiorrhiza Bunge by high-speed counter-current chromatography without presaturation of the two-phase solvent mixture

2009 ◽  
Vol 33 (1) ◽  
pp. 67-73 ◽  
Author(s):  
Dingfang Wu ◽  
Xinhang Jiang ◽  
Shihua Wu
Keyword(s):  
High Speed ◽  
Salvia Miltiorrhiza ◽  
Solvent Mixture ◽  
Salvia Miltiorrhiza Bunge ◽  
Two Phase ◽  
Counter Current

Separation of tanshinones from Salvia miltiorrhiza Bunge by high-speed counter-current chromatography using stepwise elution

2000 ◽  
Vol 904 (1) ◽  
pp. 107-111 ◽  
Author(s):  
Guilian Tian ◽  
Yabin Zhang ◽  
Tianyou Zhang ◽  
Fuquan Yang ◽  
Yoichiro Ito
Keyword(s):  
High Speed ◽  
Salvia Miltiorrhiza ◽  
Salvia Miltiorrhiza Bunge ◽  
Counter Current

scholarly journals Separation of Five Flavonoids from Aerial Parts of Salvia Miltiorrhiza Bunge Using HSCCC and Their Antioxidant Activities

Molecules ◽  
2019 ◽  
Vol 24 (19) ◽  
pp. 3448 ◽  
Author(s):  
Fan Yang ◽  
Yingxue Qi ◽  
Wei Liu ◽  
Jia Li ◽  
Daijie Wang ◽  
...  
Keyword(s):  
High Speed ◽  
Antioxidant Activities ◽  
Salvia Miltiorrhiza ◽  
Natural Antioxidants ◽  
2D Nmr ◽  
Solvent System ◽  
Positive Control ◽  
Salvia Miltiorrhiza Bunge ◽  
Two Phase ◽  
Aerial Parts

The aerial parts of Salvia miltiorrhiza Bunge, as the non-medicinal parts, are always discarded during harvesting, resulting in a huge waste of resources and environmental pressure. Due to the high flavonoid content and their antioxidant activities characteristics, the aerial parts of S. miltiorrhiza can be developed into natural antioxidants and used in foods. A high-speed counter-current chromatography (HSCCC) method, using a two-phase solvent system composed of tert-butyl methyl ether/n-butanol/acetonitrile/water (3:1:1:20, v/v), was the first to successfully isolate five flavonoids from the aerial parts of S. miltiorrhiza in one attempt, and separately categorized as rutin (1), isoquercitrin (2), kaempferol-3-O-α-l-rhamnopyranosyl-(1→6)-β-d-glucopyranoside (3), kaempferol-3-O-β-d-glucopyranoside (4) and apigenin-7-O-β-d-glucopyranoside (5) after identification. The purities of these plant isolates were 97.3%, 99.5%, 92.8%, 98.1% and 98.7%, respectively. All the flavonoids were identified by HR-ESI-MS, 1D and 2D NMR. Compounds 3 and 5 were firstly isolated from the plant of S. miltiorrhiza. Results from antioxidant assays showed that rutin (1) and isoquercitrin (2) had higher antioxidant capacities compared to L-ascorbic acid as the positive control.

Extraction and preparative purification of tanshinones from Salvia miltiorrhiza Bunge by high-speed counter-current chromatography

2011 ◽  
Vol 879 (21) ◽  
pp. 1899-1904 ◽  
Author(s):  
Ailing Sun ◽  
Yongqing Zhang ◽  
Aifeng Li ◽  
Zhaoling Meng ◽  
Renmin Liu
Keyword(s):  
High Speed ◽  
Salvia Miltiorrhiza ◽  
Salvia Miltiorrhiza Bunge ◽  
Counter Current
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