scholarly journals Effect of Nanocrystallization of Anthocyanins Extracted from Two Types of Red-Fleshed Apple Varieties on Its Stability and Antioxidant Activity

Molecules ◽  
2019 ◽  
Vol 24 (18) ◽  
pp. 3366 ◽  
Author(s):  
Jihua Xu ◽  
Xinxin Li ◽  
Shifeng Liu ◽  
Peilei Zhao ◽  
Heqiang Huo ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Corn Starch ◽  
Uv Light ◽  
Radical Scavenging ◽  
Malus Sieversii ◽  
Total Anthocyanins ◽  
High Antioxidant Activity ◽  
Starch Nanoparticles ◽  
The Stability

Red-fleshed apple (Malus sieversii f. neidzwetzkyana (Dieck) Langenf) has attracted more and more attention due to its enriched anthocyanins and high antioxidant activity. In this study we extracted total anthocyanins and phenols from two types of red-fleshed apples—Xinjing No.4 (XJ4) and Red Laiyang (RL)—to study the stability and antioxidant activity of anthocyanins after encapsulation onto Corn Starch Nanoparticles (CSNPs). The results indicated the anthocyanins and total phenol levels of XJ4 were 2.96 and 2.25 times higher than those of RL respectively. The anthocyanin concentration and loading time had a significant effect on CSNPs encapsulation, and XJ4 anthocyanins always showed significantly higher loading capacity than RL. After encapsulation, the morphology of RL-CSNPs and XJ4-CSNPs was still spherical with a smooth surface as CSNPs, but the particle size increased compared to CSNPs especially for RL-CSNPs. Different stress treatments including UV light, pH, temperature, and salinity suggested that XJ4-CSNPs exhibited consistently higher stability than RL-CSNPs. A significantly enhanced free radical scavenging rate under stress conditions was observed, and XJ4-CSNPs had stronger antioxidant activity than RL-CSNPs. Furthermore, XJ4-CSNPs exhibited a slower released rate than RL-CSNPs in simulated gastric (pH 2.0) and intestinal (pH 7.0) environments. Our research suggests that nanocrystallization of anthocyanins is an effective method to keep the anthocyanin ingredients intact and active while maintaining a slow release rate. Compared to RL, encapsulation of XJ4 anthocyanins has more advantages, which might be caused by the significant differences in the metabolites of XJ4. These findings give an insight into understanding the role of nanocrystallization using CSNPs in enhancing the antioxidant ability of anthocyanins from different types of red-fleshed apples, and provide theoretical foundations for red-fleshed apple anthocyanin application.

scholarly journals Enhancement of Chemical Stability and Dermal Delivery of Cordyceps militaris Extracts by Nanoemulsion

Nanomaterials ◽  
2020 ◽  
Vol 10 (8) ◽  
pp. 1565 ◽  
Author(s):  
Pachabadee Marsup ◽  
Kankanit Yeerong ◽  
Waranya Neimkhum ◽  
Jakkapan Sirithunyalug ◽  
Songyot Anuchapreeda ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Chemical Stability ◽  
Radical Scavenging Activity ◽  
Skin Irritation ◽  
Radical Scavenging ◽  
Water Extract ◽  
Cordyceps Militaris ◽  
Alpha Tocopherol ◽  
Dermal Delivery ◽  
The Stability

This study aimed to develop nanoemulsions for enhancing chemical stability and dermal delivery of Cordyceps militaris extracts. C. militaris was extracted by maceration and infusion. The extracts were investigated for cordycepin, phenolic, and flavonoid content. The antioxidant activity was investigated by in vitro spectrophotometric methods. The irritation profile was investigated by hen’s egg-chorioallantoic membrane test. Nanoemulsions were developed using high-pressure homogenizer. C. militaris extract was incorporated into the nanoemulsion and investigated for safety, release profile, permeation, and skin retention. The results demonstrated that water extract (CW) contained the significantly highest content of cordycepin, phenolics, and flavonoids, which were responsible for antioxidant activity. CW was the most potent antioxidant. CW possessed comparable 2,2′-diphenyl-1-picrylhydrazyl radical scavenging activity and lipid peroxidation inhibition to l-ascorbic acid (96.9 ± 3.1%) and alpha-tocopherol (87.2 ± 1.0%). Consequently, ten mg/mL of CW was incorporated into nanoemulsions composing of sugar squalene, Tween® 85, and deionized water. Nanoemulsion, which had the smallest internal droplet size (157.1 ± 2.6 nm), enhanced the stability of CW, had no cytotoxicity effect and no skin irritation, released the most CW (0.9 ± 0.0% w/w after 24 h), and delivered the highest CW into the skin layer (33.5 ± 0.7% w/w). Therefore, nanoemulsion was suggested for enhancing the stability and dermal delivery of CW.

scholarly journals Analysis of Polyphenolic Content and Antioxidant Activity of Four Dark Skin Grapes

2020 ◽  
Vol 145 ◽  
pp. 01005
Author(s):  
Yanqiu Shen ◽  
Wanling Zhang ◽  
Xiaohan Wei ◽  
Guihong Zhou ◽  
Hui Xia ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging ◽  
Chengdu Plain ◽  
Table Grapes ◽  
Polyphenolic Content ◽  
Total Anthocyanins ◽  
Total Flavonoids Content ◽  
Grape Skins ◽  
Phenolics Content ◽  
Radical Scavenging Ability

The polyphenolic of grape skins, pulps and seeds are potential source of natural antioxidant, which are receiving increased attention. In the present work, the total phenolics content (TPC), total flavonoids content (TFC), total flavanols content (TFAC), total anthocyanins content (TMAC) and antioxidant activity (DPPH, ABTS and FRAP) in different tissues of 4 table grapes cultivated in Chengdu plain, south of China, were investigated. In present study, expect TMAC was not be detected in seeds, and our results showed that seeds were the highest in polyphenolic content and antioxidant activity followed by skins, and then pulp. Besides, ‘Fenghou’ is richer in polyphenolic content than other three cultivars. Hence, ‘Fenghou’ extracts possesses higher free radical scavenging ability.

scholarly journals The Role of C-8 OH on the Antioxidant Activity of Norwogonin and Isowogonin

2020 ◽  
Vol 15 (5) ◽  
pp. 1934578X2092488
Author(s):  
Qiushan Zhang ◽  
Jin Shao ◽  
Tong Zhao ◽  
Lei He ◽  
Huiping Ma ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ferrous Iron ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Iron Chelation ◽  
Reducing Power ◽  
Antioxidant Property ◽  
Hydroxyl Group ◽  
Total Antioxidant

In the present study, the antioxidant property of 4 flavones (moslosooflavone, wogonin, isowogonin, and norwogonin) was evaluated using 6 different assays: 1,1-diphenyl-2-picrylhydrazyl (DPPH·), superoxide (O2 •−), and nitric oxide (NO) radical scavenging assays, ferrous iron chelation, reducing power, and total antioxidant capacity. The 4 flavones exhibited antioxidant activities with decreasing order as norwogonin > isowogonin >> wogonin> moslosooflavone. The present results demonstrated that norwogonin and isowogonin exhibited excellent antioxidant activity, which was mainly based on the presence of C-8 hydroxyl group.

A DFT STUDY ON THE ROLE OF DIFFERENT OH GROUPS IN THE RADICAL SCAVENGING PROCESS

2012 ◽  
Vol 11 (04) ◽  
pp. 871-893 ◽  
Author(s):  
K. SADASIVAM ◽  
R. JAYAPRAKASAM ◽  
R. KUMARESAN
Keyword(s):  
Antioxidant Activity ◽  
Density Functional ◽  
Dominant Role ◽  
Food Additives ◽  
Radical Scavenging ◽  
Theory Approach ◽  
Oh Groups ◽  
Flavonoid Compounds ◽  
Dissociation Enthalpy

The molecular properties of robinetin and melanoxetin which are the two naturally occurring flavonoid compounds have been studied theoretically by means of density functional theory approach (DFT) at the level of B3LYP/6-311G(d,p). The analysis of computed bond dissociation enthalpy (BDE), proton dissociation enthalpy (PDE), proton affinity (PA), electron transfer enthalpy (ETE) values for both the flavonoid compounds indicate the role of B-ring for the significant antioxidant characteristics and the instability of the A-ring. It also concerns the dominant role of BDE mechanism for antioxidant activity than PDE, PA and ETE mechanisms. Ionization potential (IP) is also found to be trustworthy in the study of antioxidant activity and the computed IP magnitudes are in agreement with the values of synthetic food additives. Further, the various molecular descriptors along with the plot of frontier molecular orbitals and Mulliken spin population analysis have been obtained and the validity of Koopmans' theorem is also verified with reference to antioxidant behavior.

scholarly journals Mongolian crude propolis content analysis and antioxidant activity

2019 ◽  
Vol 27 (02) ◽  
pp. 34-39
Author(s):  
Ichinkhorloo P ◽  
Gantsetseg G ◽  
Selenge Kh ◽  
Undrakhbayar Ts ◽  
Otgonsugar P ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Amino Acid ◽  
Radical Scavenging ◽  
Antioxidant Effect ◽  
Chemical Components ◽  
Total Amino Acid ◽  
Geographic Origins ◽  
Chemical Content ◽  
High Antioxidant Activity ◽  
Dpph Radical Scavenging

Propolis, a precious bee product has been widely used for pharmaceutical purposes for centuries. Chemical components, properties, and activities are differed based on geographic origins, respective plant sources, and bee species. In our research, Mongolian propolis from 2 different places was examined for chemical content and antioxidant effect. The samples were collected from Northern and Central parts of Mongolia and were examined for a total of 8 contents. Crude Mongolian propolis sample from central part of Mongolia displayed higher results in every aspect with total moisture of 2.1%; ash 1.7%; fat 67.8, 69.3%; and 1.05% of protein. The free amino acid in 70% EtOH was higher in sample from northern part with 1.21µg/mg and the total amino acid was quantified by HPLC and highest concentration detected in methionine 1.90mg/g, and 2.62 mg/g. Totally 3 alkaloids and 2 phenolic were detected by TLC and high antioxidant activity was presented in DPPH radical scavenging test. The results suggest that the best solvent for propolis samples is EtOH 70% solution. The test propolis samples contain different types of alkaloids and phenolic, have high amount of methionine and has high antioxidant effect. 

scholarly journals Characterization of The Physicochemical Properties, Antioxidant Activity, And Antiproliferative Activity of Natural Melanin From S. Reiliana

2021 ◽  
Author(s):  
Ming Lu ◽  
Mengxi Xie ◽  
Xin Fu ◽  
Miao Yu ◽  
Taiyuan Shi
Keyword(s):  
Antioxidant Activity ◽  
Physicochemical Properties ◽  
Food Additives ◽  
Radical Scavenging ◽  
Hydroxyl Groups ◽  
Health Food ◽  
Sporisorium Reilianum ◽  
High Antioxidant Activity ◽  
Radical Scavenging Ability ◽  
Dopa Melanin

Abstract This study aimed to characterize the physicochemical properties and stability of L-25 melanin extracted from Sporisorium reilianum (S. reiliana). The results showed that the maximum absorption wavelength of melanin was 215 nm. Reducing agents, heat, light, microwaving, oxidants, and common food additives did not affect the melanin. Additionally, it has a good metal stability except Mn2+. The IR spectra revealed the presence of O-H, N-H, C=O, and C=C bonds as well as carboxyl, alcohol hydroxyl, and phenolic hydroxyl groups and a pyran ring. L-25 melanin could be defined as DL-hydroxy phenylalanine (DOPA)-melanin. The antioxidant and antiproliferative were also measured. The melanin has a specific stability and high antioxidant activity, including a strong DPPH free radical scavenging ability, and protected damaged HepG2 cells by reducing reactive oxygen species, malondialdehyde, and lactate dehydrogenase content. In conclusion, S. reilianum represents a novel source of melanin, that could be applied to health food or food additives. Our results show that melanin from S. reilianum is a natural pigment with good stability that has a great prospect of development and application, providing a theoretical basis and methods for its further processing and development as a functional food.

scholarly journals In- vitro antioxidant and In vivo Anti-inflammatory Potentials of Marantochloa Leucantha (Marantaceae) Extracts and Fractions

2019 ◽  
Vol 18 (2) ◽  
pp. 233-240
Author(s):  
Wilfred O Obonga ◽  
Charles O Nnadi ◽  
Chinonye C Chima ◽  
Sunday N Okafor ◽  
Edwin O Omeje
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging ◽  
Paw Edema ◽  
Frap Assay ◽  
Rat Paw Edema ◽  
Etoac Fraction ◽  
High Antioxidant Activity ◽  
Anti Inflammatory

This study evaluated the antioxidant and anti-inflammatory properties of Marantochloa leucantha (Marantaceae). The in vitro antioxidant activity of the extracts and solvent fractions was evaluated by 1,1-diphenyl- 2-picrylhydrazyl (DPPH) radical scavenging and ferric reducing antioxidant potential (FRAP) assay models and in vivo anti-inflammatory activity by the rat paw edema model. The phytochemical screening indicated the presence of tannins, terpenoids, steroids, flavonoids, reducing sugar and phenolics. The antioxidant assay showed that all the extracts exhibited high antioxidant activity comparable with ascorbic and gallic acid controls. In DPPH model, a 250 μg/ml EtOAc fraction of the leaves showed antioxidant activity of 93.9 ± 1.7 % (EC50 0.82 μg/ml) and a 1000 μg/ml of same stem fraction produced 91.9 ± 0.3 % activity (EC50 1.38 μg/ml). In the FRAP model, EtOAc fraction exhibited 31.1±0.7 and 92.0 ± 2.2 μM Fe2+/g of dried leaves and stem, respectively at 1000 μg/ml FeSO4 equivalent. The anti-inflammatory potential of the plant showed that the crude stem extract and fractions at 200 - 600 mg/kg exhibited significant (p < 0.01) dose-related inhibition of paw edema in rats. A 200 mg/kg EtOAc fraction showed 18.8 % inhibition compared to 31 % observed in diclofenac-treated rats in 2 h post albumin challenge. These findings validated the folkloric use of this plant in the treatment of diseases associated to the oxidative stress and could further provide promising lead compounds with potent antioxidant and anti-inflammatory activities Dhaka Univ. J. Pharm. Sci. 18(2): 233-240, 2019 (December)

Triethanolamine as an Additive in the Electrophoretic Deposition of TiTe3O8 Thick Films

2012 ◽  
Vol 507 ◽  
pp. 27-34
Author(s):  
Xin Ming Su ◽  
Ai Ying Wu ◽  
Paula M. Vilarinho
Keyword(s):  
Zeta Potential ◽  
Electrophoretic Deposition ◽  
Uv Light ◽  
Thick Films ◽  
Solid State Reaction Method ◽  
Conventional Solid State Reaction ◽  
Si Substrates ◽  
Improved Stability ◽  
The Stability

A successful electrophoretic deposition (EPD) markedly depends on the stability of the suspension. In this study the role of Triethanolamine (TEA) as a stabilizer in EPD of thick films of TiTe3O8 is presented. TiTe3O8 powders were synthesized via a conventional solid-state-reaction method and dispersed in acetone with and without TEA. The stability of the suspensions was addressed by zeta-potential, UV light and FTIR measurements. The specific adsorption of TEA to TiTe3O8 particles results in a high zeta potential and improved stability of the suspensions, allowing the preparation of high quality TiTe3O8 thick films on Pt coated Si substrates. TiTe3O8 films sintered at 700 °C are dense and homogeneous.

Application of Response Surface Methodology to Optimise Preparation High Antioxidant Activity Product from Pinctada fucata Muscle

2011 ◽  
Vol 396-398 ◽  
pp. 1341-1348 ◽  
Author(s):  
Yan Yan Wu ◽  
Lai Hao Li ◽  
Zhen Hua Duan ◽  
Xian Qing Yang ◽  
Jun Shang ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Response Surface Methodology ◽  
Response Surface ◽  
Radical Scavenging ◽  
Response Surfaces ◽  
Pinctada Fucata ◽  
Degree Of Hydrolysis ◽  
Hydrolysis Time ◽  
Activity Product ◽  
High Antioxidant Activity

The high antioxidant activity product was isolated from Pinctada fucata muscle by a method of Alcalase hydrolysis. Optimization using response surface methodology was performed and 3D response surfaces were plotted from the mathematical model. The results indicated that the optimal extraction conditions were as follows: pH 7.0, temperature 61°C, E/S 3.01%, hydrolysis time 3h, under the conditions of hydrolysis products of the DPPH radical scavenging rate of 67.3% and the degree of hydrolysis of 31.2%, which was in good agreement with the predicted model value.

HPTLC analysis and in vitro antioxidant activity of aqueous bark extract of Erythrina variegata L.

2016 ◽  
Vol 63 (3) ◽  
pp. 143-157 ◽  
Author(s):  
Kanakasabapathy Devaki
Keyword(s):  
Antioxidant Activity ◽  
Secondary Metabolites ◽  
High Performance ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Bark Extract ◽  
Erythrina Variegata ◽  
High Antioxidant Activity ◽  
Layer Chromatography

Erythrina variegata L. is an important medicinal plant used in the preparations of Ayurvedic formulations used against several ailments. This study was carried out to investigate the presence of secondary metabolites using phytochemical screening, high-performance thin-layer chromatography (HPTLC) fingerprinting analysis and the antioxidant potential of the aqueous bark extract of E. variegata L. The secondary metabolites and the free radical scavenging activity were analyzed using standard protocols. The results obtained in the present study revealed that E. variegata has high antioxidant activity against free radicals based on phytoconstituents.

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