scholarly journals Anti-Haemophilus Activity of Selected Essential Oils Detected by TLC-Direct Bioautography and Biofilm Inhibition

Molecules ◽  
2019 ◽  
Vol 24 (18) ◽  
pp. 3301 ◽  
Author(s):  
Viktória Lilla Balázs ◽  
Barbara Horváth ◽  
Erika Kerekes ◽  
Kamilla Ács ◽  
Béla Kocsis ◽  
...  
Keyword(s):  
Thin Layer ◽  
Essential Oils ◽  
Thin Layer Chromatography ◽  
Absolute Ethanol ◽  
Biofilm Inhibition ◽  
Cinnamon Oil ◽  
Cinnamon Bark ◽  
Direct Bioautography ◽  
Nano Emulsion ◽  
Layer Chromatography

Essential oils (EOs) are becoming increasingly popular in medical applications because of their antimicrobial effect. Direct bioautography (DB) combined with thin layer chromatography (TLC) is a screening method for the detection of antimicrobial compounds in plant extracts, for example, in EOs. Due to their lipophilic character, the common microbiological assays (etc. disk diffusion) could not provide reliable results. The aim of this study was the evaluation of antibacterial and anti-biofilm properties of the EO of cinnamon bark, clove, peppermint, thyme, and their main components against Haemophilus influenzae and H. parainfluenzae. Oil in water (O/W) type Pickering nano-emulsions stabilized with silica nanoparticles from each oil were prepared to increase their water-solubility. Samples with Tween80 surfactant and absolute ethanol were also used. Results showed that H. influenzae was more sensitive to the EOs than H. parainfluenzae (except for cinnamon bark oil). In thin layer chromatography-direct bioautography (TLC-DB) the ethanolic solutions of thyme oil presented the best activity against H. influenzae, while cinnamon oil was the most active against H. parainfluenzae. Pickering nano-emulsion of cinnamon oil inhibited the biofilm formation of H. parainfluenzae (76.35%) more efficiently than samples with Tween80 surfactant or absolute ethanol. In conclusion, Pickering nano-emulsion of EOs could inhibit the biofilm production effectively.

scholarly journals Detection and Identification of Antibacterial and Antioxidant Components of Essential Oils by TLC-Biodetection and GC-MS

2016 ◽  
Vol 11 (11) ◽  
pp. 1934578X1601101 ◽  
Author(s):  
Ágnes M. Móricz ◽  
Györgyi Horváth ◽  
Andrea Böszörményi ◽  
Péter G. Ott
Keyword(s):  
Essential Oils ◽  
Solid Phase ◽  
Radical Scavenging ◽  
Gas Chromatography Mass Spectrometry ◽  
Cinnamon Bark ◽  
Scavenging Effect ◽  
Detection And Identification ◽  
Radical Scavenging Effect ◽  
Direct Bioautography ◽  
Layer Chromatography

Components of cinnamon bark, rosemary, clove and thyme essential oils were screened for antioxidant and antibacterial activity utilizing thin-layer chromatography (TLC) coupled with the DPPH• test and direct bioautography using Bacillus subtilis cells. The compounds in the active chromatographic zones were identified by solid-phase microextraction-gas chromatography-mass spectrometry (SPME-GC-MS) after their elution. Seven antibacterial components were found: cinnamaldehyde and eugenol in cinnamon bark oil, 1,8-cineole, camphor, borneol and α-terpineol in rosemary oil, eugenol in clove oil and thymol in thyme oil. Only two of them, thymol and eugenol displayed a free radical scavenging effect.

Purification of vetiver alcohols and esters for quantitative high-performance thin-layer chromatography determination in Haitian vetiver essential oils and vetiver acetates

2012 ◽  
Vol 1241 ◽  
pp. 103-111 ◽  
Author(s):  
Lionel Paillat ◽  
Christine Périchet ◽  
Jean-Philippe Pierrat ◽  
Sophie Lavoine ◽  
Jean-Jacques Filippi ◽  
...  
Keyword(s):  
Thin Layer ◽  
Essential Oils ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Layer Chromatography

Bioguided identification of antimicrobial compounds from Chrysopogon zizaniodes (L.) Roberty root essential oil

2019 ◽  
Vol 14 (14) ◽  
pp. 1179-1189
Author(s):  
Román Yesid Ramírez-Rueda ◽  
Jane Marinho ◽  
Marcos José Salvador
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oil ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Relevant Compound ◽  
Vancomycin Resistant ◽  
Direct Bioautography ◽  
Layer Chromatography ◽  
Root Essential Oil

Aim: To determine the group of compounds from Chrysopogon zizaniodes root essential oil that have antimicrobial activity. Materials & methods: Thin-layer chromatography coupled to direct bioautography was used to determinate the fraction(s) having antimicrobial activity against methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant Enterococcus faecalis (VREF). Through GC-MS identification, the fractions with the greatest similarity to the active thin-layer chromatography fraction were used to determinate the MIC. Results: The subfraction 8 from column chromatography was responsible for the best MIC for MRSA (62.5 μg/ml) and VREF (125 μg/ml). Five compounds possibly responsible for antimicrobial activity were preliminary identified. Conclusion: We suggest that Cedr-8-en-13-ol, could be the more relevant compound involved in the antimicrobial activity in this study.

scholarly journals ÓLEOS ESSENCIAIS E VEGETAIS: MATÉRIAS-PRIMAS PARA FABRICAÇÃO DE BIOPRODUTOS NAS AULAS DE QUÍMICA ORGÂNICA EXPERIMENTAL

Química Nova ◽  
2021 ◽  
Author(s):  
Catarina Bruno ◽  
Márcia Almeida
Keyword(s):  
Organic Chemistry ◽  
Green Chemistry ◽  
Thin Layer ◽  
Essential Oils ◽  
Thin Layer Chromatography ◽  
Vegetable Oils ◽  
Raw Materials ◽  
Natural Dye ◽  
Class 1 ◽  
Layer Chromatography

ESSENTIAL AND VEGETABLE OILS: RAW MATERIALS FOR THE MANUFACTURE OF BIOPRODUCTS IN CLASSES IN EXPERIMENTAL ORGANIC CHEMISTRY. A schedule was prepared for three undergraduate classes for the discipline of experimental organic chemistry for obtaining bioproducts. As natural raw materials, lavender flowers and cinnamon peels were used to obtain hydrolates and essential oils (Class 1), coconut and avocado pulp to obtain vegetable oils and annatto seeds to obtain a natural dye (Class 2 ). Cosmetic bioproducts (Class 3), body emulsions similar to those found on the market, were obtained from the mixture of essential and vegetable oils and the addition of natural dye. The raw materials obtained can also be used in other classes of the discipline, complying with the principles of Green Chemistry. Essential oils, for example, can be used as samples for Thin Layer Chromatography (TLC) classes and vegetable oils for the production of biodiesel.

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