scholarly journals Characterisation and screening of antimicrobial essential oil components against clinically important antibiotic-resistant bacteria using thin layer chromatography-direct bioautography hyphenated with GC-MS, LC-MS and NMR

2018 ◽  
Vol 30 (2) ◽  
pp. 121-131 ◽  
Author(s):  
Lucy Owen ◽  
Alex W. White ◽  
Katie Laird
Keyword(s):  
Essential Oil ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Resistant Bacteria ◽  
Antibiotic Resistant Bacteria ◽  
Antibiotic Resistant ◽  
Oil Components ◽  
Direct Bioautography ◽  
Layer Chromatography

Bioguided identification of antimicrobial compounds from Chrysopogon zizaniodes (L.) Roberty root essential oil

2019 ◽  
Vol 14 (14) ◽  
pp. 1179-1189
Author(s):  
Román Yesid Ramírez-Rueda ◽  
Jane Marinho ◽  
Marcos José Salvador
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oil ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Relevant Compound ◽  
Vancomycin Resistant ◽  
Direct Bioautography ◽  
Layer Chromatography ◽  
Root Essential Oil

Aim: To determine the group of compounds from Chrysopogon zizaniodes root essential oil that have antimicrobial activity. Materials & methods: Thin-layer chromatography coupled to direct bioautography was used to determinate the fraction(s) having antimicrobial activity against methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant Enterococcus faecalis (VREF). Through GC-MS identification, the fractions with the greatest similarity to the active thin-layer chromatography fraction were used to determinate the MIC. Results: The subfraction 8 from column chromatography was responsible for the best MIC for MRSA (62.5 μg/ml) and VREF (125 μg/ml). Five compounds possibly responsible for antimicrobial activity were preliminary identified. Conclusion: We suggest that Cedr-8-en-13-ol, could be the more relevant compound involved in the antimicrobial activity in this study.

scholarly journals Studies on the Antibacterial Activity and Chemical Composition of Methanol Extract of Cochlospermum tinctorium Root

2019 ◽  
pp. 1-11
Author(s):  
R. Abdulaziz ◽  
M. H. Usman ◽  
U. B. Ibrahim ◽  
B. M. Tambari ◽  
A. Nafiu ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Plant Extract ◽  
Methanol Extract ◽  
Antibiotic Resistant ◽  
Food Borne Pathogens ◽  
Food Borne ◽  
Layer Chromatography

The aim of study was to evaluate the antibacterial activity of Cochlospermum tinctorium against ten (10) strains of antibiotic resistant food-borne pathogens Staphylococcus aureus and Listeria monocytogene. Ten (10) strains of antibiotic resistant food-borne pathogens Staphylococcus aureus and Listeria monocytogene procured from Microbiology Research Laboratory Usman Danfodiyo University Sokoto. The roots of Cochlospermum tinctorium were collected from the rock side in Dambu Gomo, Zuru Local Government Area of Kebbi State, Nigeria. The roots were washed, air-dried and milled to powder using mortal and pestle and sieved to obtained fine powder. Maceration was used for extraction using methanol as solvent. The antibacterial activity of the plant was determined on Mueller Hinton agar using agar well diffusion method. Minimum concentration (MIC) and minimum inhibitory concentration (MBC) of plant extract was also determined. Thin layer chromatography and column chromatography was employed for separation and fraction of different compounds in the plant extract. The fractions were screened for antibacterial activity and active fractions having high antibacterial activity were subjected Gas Chromatography Mass Spectoscopy (GC-MS) analysis. The result of methanol extraction yield 5.17% extracts. The methanol extract of Cochlospermum tinctorium was effective in inhibiting the isolates at high concentration of 10 mg/mL. The results thin layer chromatography revealed four spots with Rf values 0.02, 0.37, 0.44 and 0.80 respectively. The GC-MS analysis of the active methanol extract of Cochlospermum tinctorium root powder revealed the existence of major peaks 1-(+)-Ascorbic acid 2,6-dihexadecanoate (R.T: 13.666), Diethyl phthalate (R.T: 10.440), Undecyl acetate (R.T: 10.007), 3-tetradecanone (R.T: 9.793), 3-hexadecanone (R.T: 12.427). It therefore concluded that the root of Cochlospermum tinctorium has immense potential to be used in the area of pharmacology as it possess antimicrobial activity against the antibiotic resistant food-borne pathogens, thus could be exploited as alternative antimicrobial drugs.

scholarly journals Anti-Haemophilus Activity of Selected Essential Oils Detected by TLC-Direct Bioautography and Biofilm Inhibition

Molecules ◽  
2019 ◽  
Vol 24 (18) ◽  
pp. 3301 ◽  
Author(s):  
Viktória Lilla Balázs ◽  
Barbara Horváth ◽  
Erika Kerekes ◽  
Kamilla Ács ◽  
Béla Kocsis ◽  
...  
Keyword(s):  
Thin Layer ◽  
Essential Oils ◽  
Thin Layer Chromatography ◽  
Absolute Ethanol ◽  
Biofilm Inhibition ◽  
Cinnamon Oil ◽  
Cinnamon Bark ◽  
Direct Bioautography ◽  
Nano Emulsion ◽  
Layer Chromatography

Essential oils (EOs) are becoming increasingly popular in medical applications because of their antimicrobial effect. Direct bioautography (DB) combined with thin layer chromatography (TLC) is a screening method for the detection of antimicrobial compounds in plant extracts, for example, in EOs. Due to their lipophilic character, the common microbiological assays (etc. disk diffusion) could not provide reliable results. The aim of this study was the evaluation of antibacterial and anti-biofilm properties of the EO of cinnamon bark, clove, peppermint, thyme, and their main components against Haemophilus influenzae and H. parainfluenzae. Oil in water (O/W) type Pickering nano-emulsions stabilized with silica nanoparticles from each oil were prepared to increase their water-solubility. Samples with Tween80 surfactant and absolute ethanol were also used. Results showed that H. influenzae was more sensitive to the EOs than H. parainfluenzae (except for cinnamon bark oil). In thin layer chromatography-direct bioautography (TLC-DB) the ethanolic solutions of thyme oil presented the best activity against H. influenzae, while cinnamon oil was the most active against H. parainfluenzae. Pickering nano-emulsion of cinnamon oil inhibited the biofilm formation of H. parainfluenzae (76.35%) more efficiently than samples with Tween80 surfactant or absolute ethanol. In conclusion, Pickering nano-emulsion of EOs could inhibit the biofilm production effectively.

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