scholarly journals Dirhamnolipid Produced by the Pathogenic Fungus Colletotrichum gloeosporioides BWH-1 and Its Herbicidal Activity

Molecules ◽  
2019 ◽  
Vol 24 (16) ◽  
pp. 2969 ◽  
Author(s):  
Zhaolin Xu ◽  
Mengying Shi ◽  
Yongqing Tian ◽  
Pengfei Zhao ◽  
Yifang Niu ◽  
...  

Fungal phytotoxins used as ecofriendly bioherbicides are becoming efficient alternatives to chemical herbicides for sustainable weed management. Previous study found that cultures of the pathogenic fungus Colletotrichum gloeosporioides BWH-1 showed phytotoxic activity. This study further isolated the major phytotoxin from cultures of the strain BWH-1 using bioactivity-guided isolation, by puncturing its host plant for an activity test and analyzing on the HPLC-DAD-3D mode for a purity check. Then, the active and pure phytotoxin was characterized as a dirhamnolipid (Rha-Rha-C10-C10) using the NMR, ESIMS, IR and UV methods. The herbicidal activity of dirhamnolipid was evaluated by the inhibition rate on the primary root length and the fresh plant weight of nine test plants, and the synergistic effect when combining with commercial herbicides. Dirhamnolipid exhibited broad herbicidal activity against eight weed species with IC50 values ranging from 28.91 to 217.71 mg L−1 and no toxicity on Oryza sativa, and the herbicidal activity could be synergistically improved combining dirhamnolipid with commercial herbicides. Thus, dirhamnolipid that originated from C. gloeosporioides BWH-1 displayed the potential to be used as a bioherbicide alone, or as an adjuvant added into commercial herbicides, leading to a decrease in herbicides concentration and increased control efficiency.

2020 ◽  
Vol 57 (3) ◽  
pp. 199-210
Author(s):  
Rajib Kundu ◽  
Mousumi Mondal ◽  
Sourav Garai ◽  
Ramyajit Mondal ◽  
Ratneswar Poddar

Field experiments were conducted at research farm of Bidhan Chandra Krishi Viswavidyalaya, Kalyani, West Bengal, India (22°97' N latitude and 88°44' E longitude, 9.75 m above mean sea level) under natural weed infestations in boro season rice (nursery bed as well as main field) during 2017-18 and 2018-19 to evaluate the herbicidal effects on weed floras, yield, non-target soil organisms to optimize the herbicide use for sustainable rice-production. Seven weed control treatments including three doses of bispyribac-sodium 10% SC (150,200, and 250 ml ha-1), two doses of fenoxaprop-p-ethyl 9.3% EC (500 and 625 ml ha-1), one weed free and weedy check were laid out in a randomized complete block design, replicated thrice. Among the tested herbicides, bispyribac-sodium with its highest dose (250 ml ha-1) resulted in maximum weed control efficiency, treatment efficiency index and crop resistance index irrespective of weed species and dates of observation in both nursery as well as main field. Similar treatment also revealed maximum grain yield (5.20 t ha-1), which was 38.38% higher than control, closely followed by Fenoxaprop-p-ethyl (625 ml ha-1) had high efficacy against grasses, sedge and broadleaf weed flora. Maximum net return (Rs. 48765 ha-1) and benefit cost ratio (1.72) were obtained from the treatment which received bispyribac-sodium @ 250 ml ha-1. Based on overall performance, the bispyribac-sodium (250 ml ha-1) may be considered as the best herbicide treatment for weed management in transplanted rice as well as nursery bed.


Weed Science ◽  
1979 ◽  
Vol 27 (5) ◽  
pp. 497-501 ◽  
Author(s):  
C. D. Boyette ◽  
G. E. Templeton ◽  
R. J. Smith

An indigenous, host-specific, pathogenic fungus that parasitizes winged waterprimrose [Jussiaea decurrens(Walt.) DC.] is endemic in the rice growing region of Arkansas. The fungus was isolated and identified asColletotrichum gloeosporioides(Penz.) Sacc. f.sp. jussiaeae(CGJ). It is highly specific for parasitism of winged waterprimrose and not parasitic on creeping waterprimrose (J. repensL. var.glabrescensKtze.), rice (Oryza sativaL.), soybeans [Glycine max(L.) Merr.], cotton (Gossypium hirsutumL.), or 4 other crops and 13 other weeds. The fungus was physiologically distinct from C.gloeosporioides(Penz.) Sacc. f. sp.aeschynomene(CGA), an endemic anthracnose pathogen of northern jointvetch[Aeschynomene virginica(L.) B.S.P.], as indicated by cross inoculations of both weeds. Culture in the laboratory and inoculation of winged waterprimrose in greenhouse, growth chamber and field experiments indicated that the pathogen was stable, specific, and virulent in a wide range of environments. The pathogen yielded large quantities of spores in liquid culture. It is suitable for control of winged waterprimrose. Winged waterprimrose and northern jointvetch were controlled in greenhouse and field tests by application of spore mixtures of CGJ and CGA at concentrations of 1 to 2 million spores/ml of each fungus in 94 L/ha of water; the fungi did not damage rice or nontarget crops.


Agronomy ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 146
Author(s):  
Leonardo F. Rocha ◽  
Karla L. Gage ◽  
Mirian F. Pimentel ◽  
Jason P. Bond ◽  
Ahmad M. Fakhoury

The soybean cyst nematode (SCN; Heterodera glycines Ichinohe) is a major soybean-yield-limiting soil-borne pathogen, especially in the Midwestern US. Weed management is recommended for SCN integrated management, since some weed species have been reported to be hosts for SCN. The increase in the occurrence of resistance to herbicides complicates weed management and may further direct ecological–evolutionary (eco–evo) feedbacks in plant–pathogen complexes, including interactions between host plants and SCN. In this review, we summarize weed species reported to be hosts of SCN in the US and outline potential weed–SCN management interactions. Plants from 23 families have been reported to host SCN, with Fabaceae including most host species. Out of 116 weeds hosts, 14 species have known herbicide-resistant biotypes to 8 herbicide sites of action. Factors influencing the ability of weeds to host SCN are environmental and edaphic conditions, SCN initial inoculum, weed population levels, and variations in susceptibility of weed biotypes to SCN within a population. The association of SCN on weeds with relatively little fitness cost incurred by the latter may decrease the competitive ability of the crop and increase weed reproduction when SCN is present, feeding back into the probability of selecting for herbicide-resistant weed biotypes. Therefore, proper management of weed hosts of SCN should be a focus of integrated pest management (IPM) strategies to prevent further eco–evo feedbacks in the cropping system.


2006 ◽  
Vol 86 (3) ◽  
pp. 875-885 ◽  
Author(s):  
J. R. Moyer ◽  
S. N. Acharya

Weeds, especially dandelion (Taraxacum officinale Weber in F.H. Wigg.), tend to infest a forage alfalfa (Medicago sativa L.) stand 2 to 4 yr after establishment. To develop better weed management systems, experiments were conducted at Lethbridge, Alberta, from 1995 to 2002 and Creston, British Columbia, from 1998 to 2001, which included the alfalfa cultivars Beaver (standard type) and AC Blue J (Flemish type) and annual applications of metribuzin and hexazinone. These herbicides are registered for weed control in irrigated alfalfa in Alberta and alfalfa grown for seed. In addition, two sulfonylurea herbicides, metsulfuron and sulfosulfuron, and glyphosate were included. All of the herbicides except glyphosate controlled or suppressed dandelion and mustard family weeds. Metsulfuron at 5 g a.i. ha-1 almost completely controlled dandelion at both locations. However, after metsulfuron application at Lethbridge, dandelion was replaced with an infestation of downy brome, which is unpalatable for cattle. None of the herbicides increased total forage (alfalfa + weed) yield, and in some instances herbicides reduced forage quality by causing a shift from a palatable to an unpalatable weed species. However, it was observed that AC Blue J consistently yielded more than Beaver, and weed biomass was consistently less in the higher-yielding cultivar. AC Blue J was developed primarily for the irrigated area in southern Alberta and for southern British Columbia. Therefore, additional experiments should be conducted to determine which alfalfa cultivars have the greatest ability to compete with weeds in other regions of western Canada. Key words: Alfalfa yield, dandelion, forage quality, weed control


1970 ◽  
Vol 33 (4) ◽  
pp. 623-629 ◽  
Author(s):  
MSA Khan ◽  
MA Hossain ◽  
M Nurul Islam ◽  
SN Mahfuza ◽  
MK Uddin

Field experiments were conducted at the research farm of Bangladesh Agricultural Research Institute, Joydebpur during kharif-1 (March to July) seasons of 2005 and 2006 to identify the critical period of crop-weed competition for Indian spinach. Major weed species were Paspalurn commersoni, Echinochlaa crusgalli. Lie nv/nc india. Cyanotis axillaris and Cyperus rotundus. The lowest weed dry matter was 76.3 g m-2 in 2005 and l01.60 g m-2 in 2006 from the plots weeded up to 40 days after transplanting (DAT). The highest yields were obtained (74.82 t ha in 2005 and 48.48 t ha in 2006) from the weed free plots. The fresh yield of Indian spinach did not vary among no weeding upto 20, 30 and 40 DAT in 2006. But weeded plot upto 30 and 40 DAT produced identical yield in 2005. Maximum BCR (4.52) was obtained from weeded plots upto 30 DAT in 2005 but BCR (2.60) was same from weeded upto 30 and 40 DA F in 2006. On an average, highest BCR (3.55) was recorded from weeding upto 30 DAT. Results revealed that the critical period of crop weed competition lies between 20 and 30 DAT and two times hand weeding would be necessary within 30 DAT for maximum benefit. Key Words: Crop-weed competitions, critical period, weed management and Indian spinach. doi: 10.3329/bjar.v33i4.2306 Bangladesh J. Agril. Res. 33(4) : 623-629, December 2008


2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
Alexandra M. Knight ◽  
Wesley J. Everman ◽  
David L. Jordan ◽  
Ronnie W. Heiniger ◽  
T. Jot Smyth

Adequate fertility combined with effective weed management is important in maximizing corn (Zea mays L.) grain yield. Corn uptake of nitrogen (N) is dependent upon many factors including weed species and density and the rate and formulation of applied N fertilizer. Understanding interactions among corn, applied N, and weeds is important in developing management strategies. Field studies were conducted in North Carolina to compare corn and weed responses to urea ammonium nitrate (UAN), sulfur-coated urea (SCU), and composted poultry litter (CPL) when a mixture of Palmer amaranth (Amaranthus palmeri S. Wats.) and large crabgrass (Digitaria sanguinalis L.) was removed with herbicides at heights of 8 or 16 cm. These respective removal timings corresponded with 22 and 28 days after corn planting or V2 and V3 stages of growth, respectively. Differences in N content in above-ground biomass of corn were noted early in the season due to weed interference but did not translate into differences in corn grain yield. Interactions of N source and N rate were noted for corn grain yield but these factors did not interact with timing of weed control. These results underscore that timely implementation of control tactics regardless of N fertility management is important to protect corn grain yield.


2015 ◽  
Vol 22 (1) ◽  
Author(s):  
Annabella T. Carcusia ◽  
Pet Roey L. Pascual ◽  
James Francienne J. Rosit

Synthetic herbicide, the most popular weed control, causes environmental hazards. The use of allelopathy in controlling weeds is a possible alternative for sustainable weed management. The study was conducted to determine the allelopathic potentials of identified invasive weed species in terms of percent and rate of germination, plant height, length of leaves and roots and percent mortality of grasses, sedges, and broadleaves. The treatments were: T0-Pre/Post-emergence Herbicide, T1Tap Water, T2-Mimosa pudica extract,T3-Lantana camara extract,T4-Chromolaena odorata extract. These were compared according to their effects on Cenchrus spinifex, Conyza canadensis, Impatiens wallerana and Cyperus rotundus. Data were analyzed using Analysis of Variance (ANOVA) for Completely Randomized Design. A further test was done using Duncans Multiple Range Test (DMRT). For germination, Lantana camara is effective in reducing the percent germination and prolonging germination rate of most common weed species. On the other hand, Chromolaena odorata is effective in reducing the percent germination and prolonging the germination rate of Impatiens wallerana, while Mimosa pudica was effective against Cenchrus spinifex and Cyperus rotundus. For growth and development, Mimosa pudica was found to be the most effective in all common weed species, except for Cyperus rotundus where Chromolaena odorata was most effective on reducing the length of leaves and roots, and above ground fresh weight. Furthermore, Chromolaena odorata was found effective against both Cyperus rotundus and Conyza canadensis while Mimosa pudica was effective against Cenchrus spinifex in terms of percent mortality. Thus, such potential of invasive weed species for weed management should be utilized. 


2020 ◽  
pp. 7-30
Author(s):  
Md. Golam Mostafa ◽  
Syed Arvin Hassan ◽  
Md. Ehsanul Haq ◽  
Md. Ahasan Habib ◽  
Kaniz Fatema ◽  
...  

A field experiment was conducted in medium fertile soil at Sher-e-Bangla Agricultural University, Dhaka, Bangladesh during November 2017 to April 2018 in Rabi season with a view to evaluate the performance of wheat varieties under different weed control methods. The experiment was carried out with three varieties i.e. BARI Gom-28, BARI Gom-29 and BARI Gom-30 in the main plot and five weed management methods viz. control (no weeding), two hand weeding at 20 and 40 DAS, Panida 33EC (Pendimethalin) @ 2000 ml ha-1 at 5 DAS pre-emergence, Afinity 50.75WP (Isoproturon) 1500 g ha-1 at 25 DAS as post-emergence herbicide and Panida 33EC (Pendimethalin) @ 2000 ml ha-1 at 5 DAS + Afinity 50.75WP (Isoproturon)1500 g ha-1 at 25 DAS in the sub plot in split plot design. Nine different major weed species were found in the field such as Cynodon dactylon, Cyperus rotundus, Echinochloa colonum, Eleusine indica, Chenopodium album, Alternanthera philoxeroides, Brassica kaber, Leliotropium indicum, Vicia sativa. Results reveled that BARI Gom-30 contributed the highest grain yield 3.01 tha-1. Pre-emergence application of Panida 33EC controlled weeds significantly which showed highest growth followed by yield achieved in wheat. BARI Gom-30 in combination with Panida 33EC produced the highest grain yield 3.52 tha-1 while the lowest grain yield 2.09 t ha-1 was obtained from BARI Gom-28 with no weeding treatment. Results reveled that Panida 33EC (pre-emergence) was found more effective to controlling weeds in wheat. Results of the study finally reveled that Panida 33EC might be considered as a feasible option for combating weed and ensuring higher yield in wheat cultivation.


Author(s):  
K. H. Shirgapure ◽  
Pritam Ghosh

Phytochemicals released by plant species into the environment inhibit the emergence and growth of surrounding plants by changing their metabolic activity or impacting on their soil community mutualists referred as allelopathy. Allelochemicals are the compounds produced from the secondary metabolism of higher plants and microorganisms such as fungi, bacteria and viruses and affect on many processes in ecosystems and agro-ecosystems.  In complex agro-ecosystem both crop and weed shows allelophathic effect. Allelochemicals from crop plant affect on other crop and weed while allelochemicals from weed effect on other weed and crop, beside this both weed and crop also shows autoallelopathy. Hence scientific and proper estimation of allelopathic plant, their allelochemical and susceptible weed species is necessary through advance research. This is helps to increase agricultural production, reduction in cost of pesticides, environment hazard, and way for the sustainable weed management and sustainable development of agricultural production as well as ecological systems.


2020 ◽  
Vol 10 ◽  
pp. 1-14
Author(s):  
Charles N. Nyamwamu ◽  
Rebecca Karanja ◽  
Peter Mwangi

This study sought to determine the relation between soil weed seed bank and weed management practices and diversity in farms in Kisii Central Sub County, Western Kenya. Eight administrative sub-locations were randomly selected. Ten farms were selected at equal distance along transect laid across each sub-location. Weed soil seed bank was assessed from soil samples collected from each of the farms; a sub-sample was taken from a composite sample of ten soil cores of 5cm diameter and 15cm deep and placed in germination trays in a greenhouse. Weed diversity in soil weed seedbank was calculated using the Shannon index (H’). Twelve weed species from 12 genera of nine families were recorded. Diversity of the weed species in soil weed seed bank was (H'=1.48). Weed management practises significantly affected weed species soil weed seedbank reserves. Use of inefficient and ineffective hand-weeding techniques resulted in high weed species diversity and abundance.


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