scholarly journals Evaluation of the Cadmium Accumulation in Tamarillo Cells (Solanum betaceum) by Indirect Electrochemical Detection of Cysteine-Rich Peptides

Molecules ◽  
2019 ◽  
Vol 24 (12) ◽  
pp. 2196
Author(s):  
Marjorie Montero-Jiménez ◽  
Lenys Fernández ◽  
José Alvarado ◽  
Mauricio Criollo ◽  
Mónica Jadán ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Low Cost ◽  
Cadmium Accumulation ◽  
Native Plant ◽  
Cadmium Intake ◽  
High Concentrations ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
Vegetables And Fruits ◽  
Solanum Betaceum

Long-term cadmium intake can be very dangerous to human health due to its toxic effects. Although people can be contaminated with this element from different sources, contaminated food is probably the most important one. Foods such as vegetables and fruits can become contaminated with cadmium existing in soils, irrigation water, or chemical fertilizers. Some plants produce an excess of cysteine-rich peptides (CRp) when affected by high concentrations of heavy metals such as cadmium, thus indicating the presence of this type of contamination. Among these plants is tamarillo (Solanum betaceum), which is locally known as “tree tomato”. This is a native plant widely consumed in the Ecuadorian Andes because of its abundance, low cost, and high content of vitamin C and fiber. The fact that Solanum betaceum produces CRp upon contamination with heavy metals means that this plant may be able to accumulate heavy metals. If this is the case, the plant can possibly be used as an indicator of metal pollution. The main goals of the present work were to evaluate the possibility of using Solanum betaceum as an indicator of metal contamination in plants and to examine its capability to accumulate metals. Both goals were met by determination of the amounts of CRp produced by Solanum betaceum cells cultivated in vitro in the laboratory under controlled conditions in the presence of different concentrations of cadmium. The CRp determination was carried out by means of electrogeneration of iodine in an iodide solution containing reduced glutathione as a biological thiol model. Solanum betaceum cells were grown in a Murashige and Skoog solution enriched with a 30 g L−1 sugar aqueous solution and 1 mg L−1 2,4-dichlorophenoxyacetic acid. The results of these experiments confirmed the following: (1) CRp production is a function of the amount of cadmium present as a contaminant up to a limiting value after which cell apoptosis occurs; (2) Solanum betaceum accumulates cadmium; (3) the analytical method used is appropriate for CRp determination; and (4) CRp determination is a valid alternative to detect contamination by heavy metals in plants.

scholarly journals Chemical Analysis and Evaluation of Antioxidant, Antimicrobial, and Photoprotective Activities of Schinopsis brasiliensis Engl. (Anacardiaceae)

2017 ◽  
Vol 2017 ◽  
pp. 1-10 ◽  
Author(s):  
Sarah Raquel Gomes de Lima-Saraiva ◽  
Fernanda Granja da Silva Oliveira ◽  
Raimundo Gonçalves de Oliveira Junior ◽  
Camila de Souza Araújo ◽  
Ana Paula de Oliveira ◽  
...  
Keyword(s):  
High Performance ◽  
Ethanolic Extract ◽  
Antimicrobial Activities ◽  
Physicochemical Analysis ◽  
Minimal Bactericidal Concentration ◽  
Native Plant ◽  
Protection Factor ◽  
Dpph Method ◽  
High Concentrations

Schinopsis brasiliensis Engl. is a native plant of Caatinga which has high concentrations of compounds capable of absorbing ultraviolet light, suggesting its potential application for the development of sunscreen preparations. After its identification and collection, this vegetable drug was submitted to a physicochemical analysis through the preparation of ethanolic extract. The phytochemical screening and analysis of extracts were carried out by high-performance liquid chromatography (HPLC) evaluation. The antioxidant activity of the extract was evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) method and β-carotene bleaching test. Inhibitory hemolytic activity and morphological deformation of erythrocytes induced by H2O2 were also demonstrated and the antimicrobial activity was analyzed by the minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) method. For the in vitro determination of the sun protection factor (SPF), the spectrophotometric method was used. From the analyses carried out with this species, this plant showed significant results for the antioxidant and antimicrobial activities, as well as sunscreen action. Important flavonoids were identified. These data are an important step for the development of new photoprotective cosmetic with Caatinga species, revealing importance and representing another incentive for the preservation of the species involved and analyzed in the study.

Australian Biosolids: Characterization and Determination of Available Copper

2004 ◽  
Vol 1 (2) ◽  
pp. 116 ◽  
Author(s):  
Ian W. Oliver ◽  
Graham Merrington ◽  
Mike J. McLaughlin
Keyword(s):  
Heavy Metals ◽  
Agricultural Land ◽  
Low Cost ◽  
Land Application ◽  
Toxic Heavy Metals ◽  
High Concentration ◽  
Protection Factor ◽  
Metal Concentrations ◽  
Total Metal ◽  
High Concentrations

Environmental Context. Land application of sewage-derived biosolids is both an inexpensive method to dispose of waste and a simple way to increase soil fertility and stability. However, biosolids often contain high concentrations of heavy metals, but not all of the metals are immediately available for uptake by the soil or other organisms. To determine if this toxicologic risk outweighs the benefits, the degree of ecologically available metal, rather than simply the entire metal content, must be known in both the as-disposed and worst conditions scenarios. Application of these principles requires regulatory bodies to amend their guidelines. Abstract. Application of biosolids to agricultural land provides a low-cost disposal option with many potential benefits to soil. However, the practice may result in accumulations of potentially toxic heavy metals, and thus regulations are in place to limit the amount of metals applied to soil in this way. Current Australian regulations are not ideal because they are based on total metal concentrations in soils and biosolids, rather than the fraction that is ecologically available (the fraction accessible by organisms). Therefore more environmentally appropriate regulations, based on the available metal portion, need to be devised. However, before this is possible, more needs to be known about the characteristics of Australian biosolids, including the factors that influence the availability of biosolid metals. Copper is a metal of great concern because of its commonly high concentration in biosolids and because of its relatively high toxicity to certain groups of bacteria and fungi. Therefore an investigation was conducted to characterize the range of properties observed in Australian biosolids, and to determine the fraction of available metals and the factors that influence it (particularly in the case of copper). General properties such as pH, electrical conductivity, organic carbon, and total metal concentrations were measured. Availability of copper was specifically measured using isotopic exchange techniques and a Cu2+ ion-selective electrode. Results showed that total copper concentration and Cu2+ activity could be used to predict available copper. A new system of biosolid land-use regulation that incorporates the available metal fraction and a pH protection factor is proposed.

Low Cost Absorbents, Techniques, and Heavy Metal Removal Efficiency

2018 ◽  
pp. 50-79
Author(s):  
Harendra Kumar Sharma ◽  
Irfan Rashid Sofi ◽  
Khursheed Ahmad Wani
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Heavy Metal Contamination ◽  
Metal Removal ◽  
Low Cost ◽  
Heavy Metal Removal ◽  
Future Research ◽  
Removal Of Heavy Metals ◽  
Living Species ◽  
High Concentrations

Heavy metal contamination in water is a serious concern to the environment and human health. High concentrations of heavy metals in the environment can be toxic to a variety of living species. Natural bio-absorbents are abundant and inexpensive and considered a waste if not managed properly. The role of bio-absorbents has been widely studied and has been utilized for the removal of heavy metals. The objective of the chapter is to search the database for different absorbents and their efficiency for the removal of heavy metals. Key words related to the study have been used to select different papers published by the researchers all over the world. A rigorous three-tier process has been utilized by the authors to select the papers from the database for the current study. This chapter has identified a few research gaps in the field of heavy metal removal by using different low cast absorbents that need to be taken into account in future research.

scholarly journals Micropropagation and callogenesis in Mandevilla guanabarica (Apocynaceae), an endemic plant from Brazil

2014 ◽  
Vol 14 (2) ◽  
pp. 108-115 ◽  
Author(s):  
Sandra Zorat Cordeiro ◽  
Naomi Kato Simas ◽  
Anaize Borges Henriques ◽  
Alice Sato
Keyword(s):  
Culture Medium ◽  
Woody Plant ◽  
Boron Concentration ◽  
Dichlorophenoxyacetic Acid ◽  
Endemic Plant ◽  
Plant Maintenance ◽  
Different Types ◽  
High Concentrations ◽  
2,4 Dichlorophenoxyacetic Acid

Mandevilla guanabarica is an endemic plant from Brazil, with pharmacological and ornamental potential, both unexplored. This study established the best culture medium for in vitro plant maintenance, efficient protocol for its regeneration, and callogenesis from different explants excised from in vitro-grown plants. Woody plant medium with double boron concentration (WPM B) plus 2.27 µM thidiazuron or 0.49 µM 2-isopentenyladenine provided multiplication rates higher than 1:6. Shoots were 100% rooted on WPM B. After acclimatization, plants showed 83% survival. For callogenesis, the use of MS media supplemented with high concentrations of picloram or 2,4-dichlorophenoxyacetic acid produced, respectively, friable or compact non-morphogenic calluses from different types of explants. This micropropagation protocol allows the production of seedlings of M. guanabarica for ornamental or commercial uses, and for conservation purposes; calluses can be used to establish suspension cultures in prospecting for bioactive compounds.

scholarly journals Removing Heavy Metals from Wastewater by using Rice Husk Wastes Fiber

2019 ◽  
Vol 8 (6) ◽  
pp. 3929-3933
Keyword(s):  
Heavy Metals ◽  
Rice Husk ◽  
Industrial Wastewater ◽  
Low Cost ◽  
Agricultural Waste ◽  
Environmental Pollutants ◽  
Agricultural Wastes ◽  
Toxic Substances ◽  
High Concentrations ◽  
Better Than

Environmental problems increase due to disposal of wastes in large quantities. Industrial wastewater is the most serious pollutants because it contains high concentrations of toxic substances and heavy metals. Agricultural wastes also cause environmental pollutants especially when it is disposed by burning. So, it is necessary to find usage of agricultural wastes as a low cost material for adsorption of heavy metals such as zinc and chromium in industrial wastewater.Rice husk fiber was used in this study to remove zinc and chromium from industrial wastewater. The obtained removal ratios obtained were 94.33% and 89.2% for Zn+2 and Cr (VI), respectively.The study showed that the removal ratio increases by increasing the adsorption contact time and decreasing the flow rate. It also showed that, the removal efficiency for zinc was better than Cr (VI) using rice husk waste fiber. The success of use the cheap adsorbent as adsorbent material from the agricultural waste in industrial wastewater treatment open the door for the existing factories to treat their wastewater with low cost that has no effect on their profits and prevent any punishment for environmental pollution

Ce1-хGdхOy Nanoparticles Stimulate Proliferation of Dental Pulp Stem Cells In Vitro

2017 ◽  
Vol 13 ◽  
pp. 26-31
Author(s):  
Anton L. Popov ◽  
Olga G. Tatarnikova ◽  
Nelly R. Popova ◽  
Irina I. Selezneva ◽  
Azamat Y. Akkizov ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cerium Oxide ◽  
Dental Pulp ◽  
Low Cost ◽  
Dependent Manner ◽  
Urgent Task ◽  
High Concentrations ◽  
Further Development ◽  
Dose Dependent

One of the main reasons for limiting the widespread clinical use of mesenchymal stem cells (MSCs) is a low speed of their proliferation in vitro. In this regard, the search for new safe and effective growth stimulants is an urgent task. In this study, we investigated the effect of nanocrystalline cerium oxide doped with gadolinium (Ce1-х Gdх Oy), on the morphofunctional characteristics and proliferative activity of MSCs derived from dental pulp. It was shown that the introduction of Ce1-х Gdх Oy nanoparticles into the culture of dental MSCs provides the activation of proliferation of the cells in a dose-dependent manner. High concentrations of Ce1-х Gdх Oy nanoparticles inhibit the proliferation of the cells; however, this does not lead to further development of apoptosis and cell death. The obtained results indicate that the nanocrystalline cerium oxide can be considered as a basis for the development of highly efficient and low-cost supplements for culturing MSCs.

scholarly journals In vitro induction of callus from cotyledon and hypocotyl explants of Glycine wightii (Wight & Arn.) Verdc.

2003 ◽  
Vol 27 (6) ◽  
pp. 1277-1284 ◽  
Author(s):  
André Luis Coelho da Silva ◽  
Cecília Sulzbacher Caruso ◽  
Renato de Azevedo Moreira ◽  
Ana Cecília Góes Horta
Keyword(s):  
Callus Induction ◽  
Basal Medium ◽  
Average Weight ◽  
Dichlorophenoxyacetic Acid ◽  
Fresh Matter ◽  
Growth Room ◽  
High Concentrations ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
In Vitro Induction

With the objective to promote in vitro callus induction, cotyledon and hypocotyl segments of "perennial soybean" (Glycine wightii (Wight & Arn.) Verdc.) were inoculated in basal medium MS supplemented with sucrose (1.5 e 3%) and 0.8% agar and different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-furfurylaminopurine (kinetin). The explants were maintained in a dark growth room at 28ºC. The best callus induction was observed in explants (cotyledon and hypocotyl) maintained in medium containing the combination of 2,4-D (1 mg.L-1), kinetin (0.1 mg.L-1) and 3% sucrose. To promote callus subculture, the MS medium was supplemented with different combinations of 2,4-D (0.5 to 4.0 mg.L-1), with or without kinetin (0.1 mg.L-1) and sucrose (1.5 e 3%). The calli were maintained 35 days in a dark growth room at 28ºC. The results indicated that the use of 2,4-D 1.0 mg.L-1 + kinetin 0.1 mg.L-1 + sucrose 3% provided the highest average weight of cotyledons calli fresh matter, whereas the use of 2,4-D 2.0 mg.L-1 + kinetin 0.1 mg.L-1 + sucrose 3% provided the highest average weight of hypocotyl calli fresh matter. High concentrations of 2,4-D, independent of kinetin and sucrose concentrations, promoted oxidation and reduction in fresh weight from calli of cotyledon and hypocotyls.

scholarly journals Removal of heavy metals derived from COD analysis wastewater by electrolysis using graphite electrode

2021 ◽  
Vol 63 (3) ◽  
pp. 85-91
Author(s):  
Thi Thanh Diem Ngo ◽  
◽  
Minh Tien Tran ◽  
Keyword(s):  
Heavy Metals ◽  
High Efficiency ◽  
Low Cost ◽  
Treatment Method ◽  
High Concentration ◽  
Graphite Electrodes ◽  
Efficient Treatment ◽  
High Concentrations ◽  
Recycle And Reuse ◽  
A Current

In this study, electrolysis using graphite electrodes was applied to treat wastewater generated from a COD analysis procedure (referred to as COD wastewater). COD wastewater containing high concentrations of H2SO4, Hg2+, and Cr2O72- salts were collected and then treated by electrolysis with graphite electrodes in a lab-scale experiment. The results showed that the electrolysis process was not affected by the electrode’s distance or area. The most efficient treatment for all three metals was achieved at a current value of 31.58 mA, which corresponds to a current density of 1.974 mA/cm2 under a voltage of 3 V, 8-h electrolysis time, wastewater pH<1, electrode distance of 4 cm, and electrode area of 16 cm2. Under these conditions, the concentrations of heavy metals after treatment were 1170.17 mg/l for Hg, 871.20 mg/l for Ag, and 56.3 mg/l for Cr. The treatment efficiencies were 48.15, 66.94, and 50.76%, for Hg, Ag, and Cr, respectively. While this technology is simple, low cost, and achieves a relatively high efficiency, after treatment the COD wastewater still carried a high concentration of heavy metals that exceeded the permissible standards. Therefore, it is necessary to have a further treatment method in place to completely eliminate the heavy metals remaining in wastewater, as well as to recycle and reuse acidic components from wastewater and to treat them up to environmental standards before discharge. In conclusion, electrolysis with graphite electrodes can be applied in practice to treat other sources of wastewater contaminated by heavy metals with low emissions.

Interaction of cytokinin, auxin, and gibberellin on peroxidase isoenzymes in tobacco tissues cultured in vitro

1972 ◽  
Vol 50 (12) ◽  
pp. 2471-2477 ◽  
Author(s):  
T. T. Lee
Keyword(s):  
Indoleacetic Acid ◽  
Polyacrylamide Gel Electrophoresis ◽  
Dichlorophenoxyacetic Acid ◽  
Tobacco Callus ◽  
Tumor Type ◽  
Low Concentrations ◽  
Most Significant Change ◽  
High Concentrations ◽  
2,4 Dichlorophenoxyacetic Acid

Peroxidase in tobacco callus tissue (Nicotiana tabacum, cv. White Gold) was resolved into three groups of isoenzymes by polyacrylamide gel electrophoresis, and a combined action of cytokinin, auxin, and gibberellin in their formation was clearly demonstrated. The most significant change was in a group of fast-migrating isoperoxidases, the development of which required both kinetin and indoleacetic acid. Kinetin was most stimulatory at 0.2 μM but became inhibitory with increasing concentrations. Indole acetic acid was effective at concentrations from 0.1 to 100 μM with an optimum at 10 μM. With both kinetin and indoleacetic acid at optimal concentrations, addition of gibberellic acid further increased the contents of the fast migrating isoperoxidases, but it was inactive in the absence of indoleacetic acid or in the presence of kinetin in 5 μM or higher concentrations. Cycloheximide, actinomycin D, and abscisic acid inhibited the formation of the fast-migrating peroxidases. Formation of the fast-migrating isoperoxidase in the tissue was associated with tumor-type growth.2,4-Dichlorophenoxyacetic acid had a dual effect on peroxidase; at low concentrations (0.1 to 1 μM) it promoted a fast-migrating isoperoxidase; at high concentrations (10 to 100 μM) it inhibited the fast-migrating isoperoxidase but caused a significant increase in other isoperoxidases of lower electrophoretic mobilities.

In Vitro Effects of Ethanol on Rabbit Platelet Aggregation, Secretion of Granule Contents, and Cyclic AMP Levels in the Presence of Prostacyclin

1989 ◽  
Vol 61 (02) ◽  
pp. 254-258 ◽  
Author(s):  
Margaret L Rand ◽  
Peter L Gross ◽  
Donna M Jakowec ◽  
Marian A Packham ◽  
J Fraser Mustard
Keyword(s):  
Cyclic Amp ◽  
Inhibitory Effect ◽  
Rabbit Platelet ◽  
Inhibitory Effects ◽  
Low Concentrations ◽  
Rabbit Platelets ◽  
High Concentrations ◽  
In Vitro Effects ◽  
Aggregation Of Platelets

SummaryEthanol, at physiologically tolerable concentrations, inhibits platelet responses to low concentrations of collagen or thrombin, but does not inhibit responses of washed rabbit platelets stimulated with high concentrations of ADP, collagen, or thrombin. However, when platelet responses to high concentrations of collagen or thrombin had been partially inhibited by prostacyclin (PGI2), ethanol had additional inhibitory effects on aggregation and secretion. These effects were also observed with aspirin- treated platelets stimulated with thrombin. Ethanol had no further inhibitory effect on aggregation of platelets stimulated with ADP, or the combination of ADP and epinephrine. Thus, the inhibitory effects of ethanol on platelet responses in the presence of PGI2 were very similar to its inhibitory effects in the absence of PGI2, when platelets were stimulated with lower concentrations of collagen or thrombin. Ethanol did not appear to exert its inhibitory effects by increasing cyclic AMP above basal levels and the additional inhibitory effects of ethanol in the presence of PGI2 did not appear to be brought about by further increases in platelet cyclic AMP levels.

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