scholarly journals Extraction Optimization, Physicochemical Characteristics, and Antioxidant Activities of Polysaccharides from Kiwifruit (Actinidia chinensis Planch.)

Molecules ◽  
2019 ◽  
Vol 24 (3) ◽  
pp. 461 ◽  
Author(s):  
Qiao-Hong Han ◽  
Wen Liu ◽  
Hong-Yi Li ◽  
Jing-Liu He ◽  
Huan Guo ◽  
...  
Keyword(s):  
Functional Food ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Physicochemical Characteristics ◽  
Extraction Techniques ◽  
Food Ingredients ◽  
Molar Ratios ◽  
Assisted Extraction ◽  
Ultrasonic Assisted

In order to evaluate effects of extraction techniques on the physicochemical characteristics and antioxidant activities of kiwifruit polysaccharides (KPS), and further explore KPS as functional food ingredients, both microwave-assisted extraction (MAE) and ultrasonic-assisted extraction (UAE) were optimized for the extraction of KPS. Furthermore, the physicochemical structures and antioxidant activities of KPS extracted by different techniques were investigated. The optimal extraction conditions of UAE and MAE for the extraction of KPS were obtained by response surface methodology. Different extraction techniques significantly affected the contents of uronic acids, molecular weights, molar ratios of constituent monosaccharides, and the degree of esterification of KPS. Results showed that KPS exhibited remarkable DPPH and ABTS radical scavenging activities, and reducing power. The high antioxidant activities observed in KPS extracted by the MAE method (KPS-M) might be partially attributed to its low molecular weight and high content of unmethylated galacturonic acid. Results suggested that the MAE method could be a good potential technique for the extraction of KPS with high antioxidant activity, and KPS could be further explored as functional food ingredients.

scholarly journals Extraction Optimization, Structural Characterization, and Antioxidant Activities of Polysaccharides from Cassia Seed (Cassia obtusifolia)

Molecules ◽  
2019 ◽  
Vol 24 (15) ◽  
pp. 2817 ◽  
Author(s):  
Ding-Tao Wu ◽  
Wen Liu ◽  
Qiao-Hong Han ◽  
Ping Wang ◽  
Xian-Rong Xiang ◽  
...  
Keyword(s):  
Functional Food ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Weight Ratio ◽  
Reducing Power ◽  
Natural Antioxidants ◽  
Hot Water ◽  
Raw Material ◽  
Food Ingredients ◽  
Chemical Structures

In order to explore Cassia seed polysaccharides (CSPs) as natural antioxidants for application in the functional-food industry, microwave-assisted extraction (MAE) was optimized for the extraction of CSPs by using a response surface methodology. Furthermore, the chemical structures and antioxidant activities of CSPs extracted by MAE and hot water extraction were investigated and compared. The maximum extraction yield of CSPs extracted by MAE (8.02 ± 0.19%) was obtained at the optimized extraction parameters as follows: microwave power (415 W), extraction time (7.0 min), and ratio of water to raw material (51 mL/g). Additionally, the contents of the uronic acids, molecular weight, ratio of constituent monosaccharides, intrinsic viscosities, and degrees of esterification of CSPs were significantly affected by the MAE method. Moreover, CSPs exhibited remarkable 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) ABTS, 2,2-diphenyl-1-(2,4,6-trinitrophenyl) hydrazyl DPPH, nitric oxide, and hydroxyl radical scavenging activities as well as reducing power. The high antioxidant activities observed in CSPs extracted by MAE could be partially attributed to its low molecular weights and high content of unmethylated galacturonic acid. Results indicate that the MAE method could be an efficient technique for the extraction of CSPs with high antioxidant activity, and CSPs could be further explored as functional food ingredients.

scholarly journals Comparison of Classical and Ultrasound-Assisted Extraction Methods on Antioxidant Activities of Pharmaceutically Active Compounds from Valeriana Officinalis

2021 ◽  
Vol 5 (2) ◽  
Keyword(s):  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Extraction Methods ◽  
Total Phenolic ◽  
Valeriana Officinalis ◽  
Assisted Extraction ◽  
Ultrasonic Assisted ◽  
Dpph Radical Scavenging

Medicinal plants are good sources and rich of substances which having nutraceutical and health benefits. Valeriana officinalis L. (Valerianaceae) is a known medicinal plant used in traditional medicine worldwide. The aim of present study was to evaluate the efficiencies of three methods for evaluated for antioxidant from Valeriana officinalis. Besides, the impact of extraction methods on total phenollic and flavonoids contents and antioxidant activities of Valeriana root was investigated. The dried-root was extracted by three different methods including maceration, ultrasonic assisted, and Soxhlet assisted extraction. Antioxidant capacity was assessed using four different methods: DPPH and nitric oxide (NO) free radicals scavenging, reducing power and iron chelating activity. The total phenolic and flavonoid contents were also identified. The ultrasonic extract showed highest amount of total phenolics and flavonoids contents. In DPPH radical scavenging activity, the ultrasonic assisted extract, (IC50=0.546 μg/ ml) had a higher activity from other extracts. In reducing power assay, maceration extract showed the highest activity. In DPPH radical scavenging activity, IC50 for ultrasonic extract, soxhlet assisted extraction and maceration extract were 0.546, 0.816 and 0.678 μg/ml, respectively. Here, the maceratin extract showed the highest activity, too. The results clearly indicated the extraction methods used in this study significantly affected antioxidant capacities and total phenolic and flavonoids contents. Ultrasonic assisted extraction and Soxhlet methods were found to be more efficient in extraction of antioxidant components of valeriana.

Effects of Ultrasound on the Physicochemical Properties and Antioxidant Activities of Chestnut Polysaccharide

2016 ◽  
Vol 12 (5) ◽  
pp. 439-449 ◽  
Author(s):  
Fan Hou ◽  
Yanwen Wu ◽  
Lina Kan ◽  
Qian Li ◽  
Shuangshuang Xie ◽  
...  
Keyword(s):  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Hot Water ◽  
Ultrasound Assisted Extraction ◽  
Scanning Calorimetry ◽  
Sem Images ◽  
Optimal Method ◽  
Assisted Extraction ◽  
Ultrasound Assisted

Abstract A comparison of chestnut polysaccharide extraction using ultrasound-assisted extraction (UAE) and hot water extraction (HWE) demonstrated that UAE is superior to HWE due to its higher extraction efficiency. Scanning electron microscopy (SEM), thermogravimetric analysis-differential scanning calorimetry (TGA-DSC) and Fourier-transform infrared spectroscopy (FTIR) were used to characterize the ultrasound-assisted-extracted polysaccharide (UAEP) and hot water-extracted polysaccharide (HWEP). SEM images revealed that the UAEP and chestnut residue were crushed, with particle sizes that were smaller than those of the HWEP, which was related to the breakage of long-chain polysaccharides. TGA-DSC showed a higher transition temperature and enthalpy value for the UAEP than the HWEP, and the FTIR spectrum revealed typical characteristics of polysaccharides, with some differences between the UAEP and HWEP. The evaluation of antioxidant activities showed that the UAEP had stronger antioxidant capacities than the HWEP, regardless of the reducing power and DPPH-, ABTS- and hydroxyl radical-scavenging activities, suggesting that ultrasound is an optimal method to rapidly extract chestnut polysaccharide, a potential natural antioxidant.

Characterization and Antioxidant Activity of Flash-Assisted Extracted Dihydroquercetin from Wood Sawdust of Larix gmelinii Using a Response Surface Methodology

2016 ◽  
Vol 12 (6) ◽  
pp. 587-597 ◽  
Author(s):  
Jianxia Li ◽  
Qiaohui Zhang ◽  
Jie Cui ◽  
Yaping Ning ◽  
Fengjun Wang ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Larix Gmelinii ◽  
Wood Sawdust ◽  
Assisted Extraction

Abstract We investigate a flash-assisted extraction of dihydroquercetin (DHQ) from wood sawdust of Larix gmelinii by response surface methodology. Box–Behnken design optimized the extraction conditions, and the highest DHQ yield (6.15 %) was obtained under optimal extraction conditions: FAE time (3 min), extraction time (3 h), solid-to-liquid (g/mL) ratio (1:15), and ethanol concentration (75 %). After purification, the DHQ purity was 96 % as determined by HPLC and was further characterized by FTIR. Purified DHQ’s antioxidant activities were confirmed in vitro by determining DPPH and ABTS radical scavenging activities and reducing Fe3+ power. In the DPPH and ABTS radical scavenging activity assays, DHQ displayed prominent antioxidant activities with low IC50 values (11.568 and 12.475 μg/mL, respectively). In reducing Fe3+ power assay, high DHQ absorbance values showed that DHQ had higher reducing power than butylated hydroxyl toluene and had nearly the same consistent power as ascorbic acid at the same doses.

scholarly journals EVALUATION OF THREE METHODS FOR THE EXTRACTION OF ANTIOXIDANTS FROM VICIA FABA L. BEAN AND HULLS

2014 ◽  
Vol 44 (3) ◽  
pp. 203-208
Author(s):  
Z. HASHEMI ◽  
M. A. EBRAHIMZADEH
Keyword(s):  
Vicia Faba ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Extraction Methods ◽  
Aluminium Chloride ◽  
Total Phenolic ◽  
Assisted Extraction ◽  
Vicia Faba L ◽  
Phenolic And Flavonoid

 The efficiencies of three extraction methods (percolation, Soxhlet and ultrasonically assisted extraction) were evaluated for the extraction of antioxidants from Vicia faba L. bean and hulls. Antioxidant activities of extracts were evaluated using four different tests. Total phenolic and flavonoid contents of the extracts were determined using the Folin–Ciocalteu and aluminium chloride methods, respectively. Although the yield of extractions for ultrasonically assisted extraction was about half to one-fourth of that of other methods, the extraction ratio of total phenol was higher. Hull extracts had higher total phenolic and flavonoid contents and antioxidant activities than bean extracts. The hull ultrasonic extract showed the best DPPH (IC50= 56.9 ± 2.5 g ml-1) and NO radical scavenging (11.3 ± 0.5 gml-1). The hull percolation extract showed the best iron chelating ability (171.8 ± 6.8 g ml-1) and reducing power. The results indicated that all extraction methods could effectively extract antioxidants from medicinal plants

Ultrasonic-Assisted Extraction and Antioxidant Activities of Polysaccharides from Radix astragali

2013 ◽  
Vol 864-867 ◽  
pp. 549-552 ◽  
Author(s):  
Ming Wu ◽  
Chuan Fu Ma ◽  
Jun Man Kan ◽  
Li Quan Guo
Keyword(s):  
Superoxide Anion ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Ultrasonic Power ◽  
Extraction Temperature ◽  
Radix Astragali ◽  
Assisted Extraction ◽  
Ultrasonic Assisted ◽  
Ultrasonic Assisted Extraction ◽  
Solid Liquid

Ultrasonic-assisted extraction (UAE) method was used to polysaccharides from Radix Astragali (RAP), and the orthogonal test was applied to optimize extraction conditions. Furthermore, the antioxidant activities of RAP were evaluated by DPPH and superoxide anion radical scavenging activities assay. The results indicated that the optimal conditions for RAP extraction as followings: solid/liquid ratios of 1:20, extraction time of 10 min, ultrasonic power of 65 W and extraction temperature of 60°C. Meanwhile, RAP had relatively higher scavenging activities on DPPH and superoxide anion radicals.

scholarly journals Ultrasonic-Assisted Extraction of Natural Yellow Pigment from Physalis pubescens L. and Its Antioxidant Activities

2018 ◽  
Vol 2018 ◽  
pp. 1-6
Author(s):  
Haitang Wang ◽  
Lin Shi ◽  
Xiaoyu Yang ◽  
Rui Hong ◽  
Liang Li
Keyword(s):  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Yellow Pigment ◽  
Ultrasonic Power ◽  
Optimal Parameters ◽  
Assisted Extraction ◽  
Ultrasonic Time ◽  
Ultrasonic Assisted ◽  
Ultrasonic Assisted Extraction

Physalis pubescens L. is rich in natural pigments but has not yet been fully utilized. Ultrasound-assisted extraction of yellow pigment from Physalis pubescens L. was investigated by response surface methodology in this study. Optimal parameters were ultrasonic power of 29.21%, ultrasonic time of 14.41 min, and ultrasonic interval time of 10.55 s. The yield was 0.193% under optimal parameters. FRAP, ABTS, and superoxide radical scavenging activity of the yellow pigment were 6.11 ± 0.22 mmol/g, 2.80 ± 0.27 mmol/g, and 57281.5 ± 2749.5 U/g, respectively. The results showed that the yield of yellow pigment could be improved by ultrasonic-assisted extraction and the yellow pigment extracted by ultrasound had antioxidant activity.

scholarly journals Response Surface Optimized Ultrasonic Assisted Extraction of Total Flavonoids from QingLi Cao and Antioxidant Activities

2015 ◽  
Vol 9 (1) ◽  
pp. 134-142 ◽  
Author(s):  
Xudong Jiang ◽  
YaoLing Liao ◽  
GuiXi Lu ◽  
Zhike Xiao
Keyword(s):  
Response Surface ◽  
Antioxidant Activities ◽  
Reducing Power ◽  
Total Flavonoids ◽  
Extraction Temperature ◽  
Ultrasound Assisted Extraction ◽  
Solid Ratio ◽  
Assisted Extraction ◽  
Ultrasonic Assisted ◽  
Ultrasonic Assisted Extraction

An ultrasound-assisted extraction technique was used to extract the total flavonoids from QingLi Cao. The optimal conditions were ethanol concentration 59.20%, liquid-to-solid ratio 31.15 mL/g, extraction time 57.42 min and extraction temperature 58.57°C, which were determined using response surface methodology. The antioxidant activities including reducing power, ABTS+, DPPH, superoxide anion and hydroxyl radical were evaluated, which suggested significant antioxidant activities.

scholarly journals Extraction Optimization and Effects of Extraction Methods on the Chemical Structures and Antioxidant Activities of Polysaccharides from Snow Chrysanthemum (Coreopsis Tinctoria)

Polymers ◽  
2019 ◽  
Vol 11 (2) ◽  
pp. 215 ◽  
Author(s):  
Huan Guo ◽  
Qin Yuan ◽  
Yuan Fu ◽  
Wen Liu ◽  
Ya-Hong Su ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Antioxidant Activities ◽  
Reducing Power ◽  
Natural Antioxidants ◽  
Extraction Methods ◽  
Hot Water ◽  
Extraction Time ◽  
Food Ingredients ◽  
Chemical Structures ◽  
Assisted Extraction

In order to explore snow chrysanthemum polysaccharides (SCPs) as functional food ingredients and natural antioxidants for industrial applications, both microwave-assisted extraction (MAE) and ultrasonic-assisted extraction (UAE) were firstly optimized for the extraction of SCPs. Furthermore, the effects of conventional hot water extraction, UAE, and MAE on the chemical structures and antioxidant activities of SCPs were investigated. The maximum extraction yields of SCPs extracted by UAE (4.13 ± 0.24%) and MAE (4.26 ± 0.21%) were achieved at the optimized extraction parameters as follows: ultrasound amplitude (68%) and microwave power (500 W), ultrasound extraction time (21 min) and microwave extraction time (6.5 min), and ratio of liquid to raw material (42.0 mL/g for UAE and 59.0 mL/g for MAE). In addition, different extraction methods significantly affected the contents of uronic acids, the molecular weights, the molar ratio of constituent monosaccharides, and the degree of esterification of SCPs. SCPs exhibited remarkable DPPH (IC50 ≤ 1.702 mg/mL), ABTS (IC50 ≤ 1.121 mg/mL), and nitric oxide (IC50 ≤ 0.277 mg/mL) radical scavenging activities, as well as reducing power (≥ 80.17 ± 4.8 μg Trolox/mg), which suggested that SCPs might be one of the major contributors toward the antioxidant activities of snow chrysanthemum tea. The high antioxidant activities (DPPH, IC50 = 0.693 mg/mL; ABTS, IC50 = 0.299 mg/mL; nitric oxide, IC50 = 0.105 mg/mL; and reducing power, 127.79 ± 2.57 μg Trolox/mg) observed in SCP-M extracted by the MAE method might be partially attributed to its low molecular weight and high content of unmethylated galacturonic acids. Results suggested that the MAE method could be an efficient technique for the extraction of SCPs with high antioxidant activity, and SCPs could be further explored as natural antioxidants for industrial application.

scholarly journals Bioassay-Guided Different Extraction Techniques of Carica papaya (Linn.) Leaves on In Vitro Wound-Healing Activities

Molecules ◽  
2020 ◽  
Vol 25 (3) ◽  
pp. 517 ◽  
Author(s):  
Husnul Hanani Soib ◽  
Hassan Fahmi Ismail ◽  
Fitrien Husin ◽  
Mohamad Hafizi Abu Bakar ◽  
Harisun Yaakob ◽  
...  
Keyword(s):  
Wound Healing ◽  
Carica Papaya ◽  
Octadecenoic Acid ◽  
Radical Scavenging ◽  
Human Skin Fibroblast ◽  
Extraction Techniques ◽  
Assisted Extraction ◽  
Ultrasonic Assisted ◽  
Ultrasonic Assisted Extraction ◽  
Conventional Methods

Herbal plants are traditionally utilized to treat various illnesses. They contain phytochemicals that can be extracted using conventional methods such as maceration, soxhlet, and boiling, as well as non-conventional methods including ultrasonic, microwave, and others. Carica papaya leaves have been used for the treatment of dengue, fungal, and bacterial infections as well as an ingredient in anti-aging products. Phytochemicals analysis detected the presence of kaempferol, myricetin, carpaine, pseudocarpaine, dehydrocarpaine I and II, ferulic acid, caffeic acid, chlorogenic acid, β-carotene, lycopene, and anthraquinones glycoside. Conventional preparation by boiling and simple maceration is practical, simple, and safe; however, only polar phytochemicals are extracted. The present study aims to investigate the effects of three different non-conventional extraction techniques (ultrasonic-assisted extraction, reflux, and agitation) on C. papaya phytochemical constituents, the antioxidant capacity, and wound-healing activities. Among the three techniques, the reflux technique produced the highest extraction yield (17.86%) with the presence of saponins, flavonoids, coumarins, alkaloids, and phenolic metabolites. The reflux technique also produced the highest 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging with an IC50 value of 0.236 mg/mL followed by ultrasonic-assisted extraction (UAE) (IC50: 0.377 mg/mL) and agitation (IC50: 0.404 mg/mL). At tested concentrations (3.125 µg/mL to 500 µg/mL), all extracts do not exhibit a cytotoxicity effect on the human skin fibroblast, HSF1184. Interestingly, reflux and UAE were active fibroblast proliferators that support 85% (12.5 µg/mL) and 41% (6.25 µg/mL) better cell growth, respectively. Additionally, during the early 24 h of the scratch assay, the migration rate at 12.5 µg/mL was faster for all extracts with 51.8% (reflux), 49.3% (agitation), and 42.5% (UAE) as compared to control (21.87%). At 48 h, proliferated cells covered 78.7% of the scratch area for reflux extract, 63.1% for UAE, 61% for agitation, and 42.6% for control. Additionally, the collagen synthesis was enhanced for 31.6% and 65% after 24 and 48 h of treatment for reflux. An HPLC-MS/MS-QTOF (quadruple time-of-flight) analysis of reflux identified nine phytochemicals, including carpaine, kaempferol 3-(2G-glucosylrutinoside), kaempferol 3-(2″-rhamnosylgalactoside), 7-rhamnoside, kaempferol 3-rhamnosyl-(1->2)-galactoside-7-rhamnoside, luteolin 7-galactosyl-(1->6)-galactoside, orientin 7-O-rhamnoside, 11-hydroperoxy-12,13-epoxy-9-octadecenoic acid, palmitic amide, and 2-hexaprenyl-6-methoxyphenol. The results suggested that reflux was the best technique as compared to ultrasonic and agitation.

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