scholarly journals Application of pH-Responsive Fucoidan/Chitosan Nanoparticles to Improve Oral Quercetin Delivery

Molecules ◽  
2019 ◽  
Vol 24 (2) ◽  
pp. 346 ◽  
Author(s):  
Ana Barbosa ◽  
Sofia Costa Lima ◽  
Salette Reis
Keyword(s):  
Antioxidant Activity ◽  
Particle Size ◽  
Self Assembly ◽  
Functional Food ◽  
Polymeric Nanoparticles ◽  
Chitosan Nanoparticles ◽  
Weight Ratio ◽  
Ph Responsive ◽  
Assembly Method ◽  
Nm Range

Polymeric nanoparticles based on fucoidan and chitosan were developed to deliver quercetin as a novel functional food. Through the polyelectrolyte self-assembly method, fucoidan/chitosan (F/C) nanoparticles were obtained with three different weight ratios (1/1, 3/1, and 5/1). The content of quercetin in the fucoidan/chitosan nanoparticles was in the range 110 ± 3 to 335 ± 4 mg·mL−1, with the increase of weight ratio of fucoidan to chitosan in the nanoparticle. Physicochemically stable nanoparticles were obtained with a particle size within the 300–400 nm range and surface potential higher than +30 mV for the 1F/1C ratio nanoparticle and around −30 mV for the 3F/1C and 5F/1C ratios nanoparticles. The 1F/1C ratio nanoparticle became larger and more unstable as the pH increased from 2.5 to 7.4, while the 3F/1C and 5F/1C nanoparticles retained their initial characteristics. This result indicates that the latter nanoparticles were stable along the gastrointestinal tract. The quercetin-loaded fucoidan/chitosan nanoparticles showed strong antioxidant activity and controlled release under simulated gastrointestinal environments (in particular for the 3F/1C and 5F/1C ratios), preventing quercetin degradation and increasing its oral bioavailability.

scholarly journals Controlling the Antioxidant Activity of Green Tea Extract through Encapsulation in Chitosan-Citrate Nanogel

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
F. Piran ◽  
Z. Khoshkhoo ◽  
S. E. Hosseini ◽  
M. H. Azizi
Keyword(s):  
Antioxidant Activity ◽  
Particle Size ◽  
Zeta Potential ◽  
Green Tea ◽  
Chitosan Nanoparticles ◽  
Green Tea Extract ◽  
X Ray Diffraction ◽  
Bioactive Ingredients ◽  
Ft Ir ◽  
Tea Extract

Applying bioactive ingredients in the formulation of foods instead of artificial preservatives is problematic because bioactive ingredients are unstable and sensitive to environmental conditions. The present study aimed to control the antioxidant activity of green tea extract (GT) through encapsulating in chitosan nanoparticles (CS-NP). The synthesized nanoparticles were analyzed by using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), and X-ray diffraction (XRD). The encapsulation efficiency (EE), particle size, zeta potential, and polydispersity index (PDI) of GT-loaded CS-nanoparticles (CS-NP-GT) were assessed. Based on the results, the particle size and zeta potential related to the ratio of CS to GT of 1 : 0.5 were obtained as 135.43 ± 2.52 nm and 40.40 ± 0.2 mV, respectively. Furthermore, the results of FT-IR and XRD confirmed the validity of encapsulating GT in CS-NP. In addition, the antioxidant activity of GT increased after nanoencapsulation since the IC50 value of CS-NP-GT decreased to 6.13 ± 0.12 μg/ml. Finally, applying these particles for delivering GT polyphenols in foods is regarded as promising.

scholarly journals Chitosan anchored nanoparticles for breast cancer: preparation and evaluation: part-I

2019 ◽  
Vol 9 (1) ◽  
pp. 1-5
Author(s):  
Rajkumari Thagele ◽  
Archana Bagre ◽  
Mohan Lal Kori
Keyword(s):  
Breast Cancer ◽  
Particle Size ◽  
Sodium Tripolyphosphate ◽  
Polymeric Nanoparticles ◽  
Research Work ◽  
Chitosan Nanoparticles ◽  
Physical Factors ◽  
Ionic Gelation ◽  
Shell Nanoparticles ◽  
Preparation And Evaluation

The objective of present research work was to develop methotrexate loaded chitosan anchored shell nanoparticles for drug delivery in breast cancer. Chitosan nanoparticles (CS-NPs) were synthesized by ionic gelation of chitosan using sodium tripolyphosphate (STPP). The optimized nanoparticles were characterized for particle size and polydispersity index (PDI) revealed particle size were found to be between 57.08 nm to169.5 nm and PDI 0.252 to 0.639 respectively. The results signpost that stirring speed during ionic gelation reaction was also decisive parameters for the size of the nanoparticles obtained. Further characterization involved to show polymer-drug interaction was FTIR and DSC. This paper grants a revision of the physical factors elaborate in attaining nanoparticles in order to regulate the particle size of polymeric nanoparticles made from chitosan, without any surplus chemical treatment. Keywords: Breast cancer, Nanoparticles, Chitosan, Methotrexate

scholarly journals Effects of Different Drying Methods on the Characterization, Dissolution Rate and Antioxidant Activity of Ursolic Acid-Loaded Chitosan Nanoparticles

Foods ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 2470
Author(s):  
Lujie Zhao ◽  
Xu Duan ◽  
Weiwei Cao ◽  
Xing Ren ◽  
Guangyue Ren ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Particle Size ◽  
Ursolic Acid ◽  
Freeze Drying ◽  
Water Solubility ◽  
Drug Loading ◽  
Chitosan Nanoparticles ◽  
Simulated Gastric Fluid ◽  
Drying Methods ◽  
Drying Time

To improve the water solubility of ursolic acid (UA), UA-loaded chitosan nanoparticles were firstly prepared by the ionotropic gelation method and dried by freeze drying (FD), microwave freeze drying (MFD) and spray drying (SD). The characterization of UA-loaded chitosan nanoparticles was performed with particle size, drug loading (DL), scanning electron microscope (SEM), fourier transform infrared spectroscopy (FT-IR), differential scanning calorimetry (DSC), dissolution studies and antioxidant activity. The results demonstrated that UA was successfully encapsulated into chitosan nanoparticles using sodium tripolyphosphate (TPP) as a cross-linker, with a 79% encapsulation efficiency. The spray-dried, UA-loaded chitosan nanoparticles had the lowest drug loading (11.8%) and the highest particle size (496.9 ± 11.20 nm). The particle size of UA-loaded chitosan nanoparticles dried by MFD and FD was lower, at 240.8 ± 12.10 nm and 184.4 ± 10.62 nm, respectively, and their antioxidant activity was higher than those nanoparticles dried by SD. Moreover, the drying time and energy consumption of UA-loaded chitosan nanoparticles dried by MFD and SD were lower than that of FD. The dissolution rates of UA-loaded chitosan nanoparticles prepared by FD and MFD were 60.6% and 57.1%, respectively, in a simulated gastric fluid, which was a greater value than SD (55.9%). Therefore, the UA-loaded chitosan nanoparticles encapsulation method, combined with MFD technology, showed a promising potential to improve the water solubility of UA.

scholarly journals Sea Grape (Ceulerpa racemosa) Cereal with Addition of Tempe as an Anti-Aging Functional Food: In Vitro Study

2021 ◽  
Vol 5 (Supplement_2) ◽  
pp. 41-41
Author(s):  
Fahrul Nurkolis ◽  
Keren Esther Kristina Mantik ◽  
Mury Kuswari ◽  
Fachruddin Perdana ◽  
Nelly Mayulu ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Particle Size ◽  
Functional Food ◽  
In Vitro Study ◽  
Isolation Technique ◽  
Funding Sources ◽  
Significant Difference ◽  
Naoh Solution ◽  
Tyrosinase Inhibitors

Abstract Objectives This study aims to determine in vitro the effect of collagen size on anti-aging activity in the form of anti-glycation, tyrosinase inhibitors and antioxidant activity in order to obtain the optimum anti-aging collagen isolation technique from sea grape cereal with the addition of tempe. Methods Sample preparation by making cereals consisting of sea grape and tempe flour into powder using a Freeze Dryer (Lyovapor ™ L-200). Collagen isolation was carried out by varying the concentration of NaOH, which are 0.10 M; 0.20 M; and 0.30 M with a ratio of 1:10 (w/v) for 48 hours, followed by immersion using 1 M acetic acid with a ratio of 1:10 (w/v) for 24 hours. Collagen obtained from the best immersion treatment with NaOH solution is dissolved in distilled water with a ratio of 1 : 2 (v/v) and the size is changed with the help of a magnetic stirrer at a speed of 1000 rpm with a time variation of 5 and 10 hours. The results were then tested for particle size using a Particle Size Analyzer (PSA) and then tested for antioxidant activity using the DPPH (2,2-diphenyl-1-picrylhydrazyl) method, anti-glycation, and tyrosinase inhibitors. Selection of the best results is based on antioxidant, anti-glycation, and anti-tyrosinase activities. The data obtained from three replications were analyzed using ANOVA at a 95% confidence interval. Results Collagen isolated with 0.10 M NaOH was the collagen with the largest particle size, yield and anti-glycation respectively 2.45 µm, 11.65%, 62.76% and had the infrared spectrum that best matched the standard collagen. Furthermore, this collagen is stirred at a speed of 1000 rpm for 5 and 10 hours for size reduction. Collagen with 5 hours stirring has a smaller particle size (1482 nm) compared to 10 hours stirring (1568 nm). Collagen with a size of 1482 nm showed the best activity, namely antioxidant activity against (DPPH) of 24.70% and tyrosinase inhibitor of 26.77%. There was a significant difference (P < 0.05) in the DPPH inhibition test and the tyrosinase inhibition of L-DOPA substrates. Conclusions Based on the anti-glycation, anti-tyrosinase and antioxidant activity of collagen by immersing 0.10 M NaOH and stirring for 5 hours, it has the best anti-aging properties. This is useful for tests in determining variations of cereal formulations that will be used as functional food products rich in anti-aging collagen. Funding Sources Personal funding from author

scholarly journals NANO KITOSAN EKSTRAK TONGKOL JAGUNG MANADO KUNING (Zea Mays L.) DAN AKTIVITAS ANTIOKSIDANNYA

2020 ◽  
Vol 12 (1) ◽  
Author(s):  
Julista Alfa Kabalmay ◽  
Edi Suryanto ◽  
Max R. J. Runtuwene
Keyword(s):  
Antioxidant Activity ◽  
Particle Size ◽  
Surface Morphology ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Chitosan Nanoparticles ◽  
Reducing Power ◽  
Total Phenolic ◽  
Yellow Corn ◽  
Micrometer Size

ABSTRAK Tujuan dari penelitian ini untuk mengetahui potensi antioksidan dari nanopartikel kitosan ekstrak tongkol jagung Manado kuning. Penelitian ini terdiri dari 2 tahap yaitu ekstraksi dan pembuatan nanopartikel kitosan. Parameter yang digunakan adalah total senyawa fenolik dan kemampuan mereduksi, ukuran partikel, indeks polidispersitas, dan morfologi permukaan nanopartikel kitosan ekstrak tongkol jagung Manado kuning. Pembuatan nanopartikel kitosan menggunakan metode gelasi ionik. Hasil penelitian menunjunkkan partikel serbuk tongkol jagung 75 µm  memiliki rendemen dan kandungan total fenolik yang lebih tinggi dibanding  ukuran 150 µm. Formula F1 nanopartikel kitosan ekstrak tongkol jagung memiliki ukuran partikel  yang lebih kecil (318,7 nm) daripada F2 (333,8 nm), dan F3  (339,9 nm).  Hasil pengamatan morfologi permukaan nanopartikel kitosan adalah berbentuk seperti gumpalan yang masih berukuran mikrometer dan bentuk bulat tidak sempurna. Hasil pengujian aktivitas antioksidan bahwa formulasi F1 memiliki aktivitas yang terbaik dibandingkan dengan F0, F2 dan F3. ABSTRACT The objective of this study was to determine the antioxidant potential of chitosan nanoparticles from corncob extracts of Manado yellow corn. The study was consist of two stages: extraction and synthesis of chitosan nanoparticles. The parameters evaluated were total phenolic content (TPC), reducing power, particle size, index of polydispersity, and surface morphology of chitosan nanoparticles from Manado yellow corncob extract. Synthesis of chitosan nanoparticles was carried out by using ionic gelation method. The result showed that corncob powder with particle size 75 μm had higher recovery yield and higher total phenolic content (150 μm) than 150 μm. Formula F1 chitosan nanoparticles extract of corncob had smaller particle size (318.7 nm) compared to F2 (333.8 nm) and F3 (339.9 nm). Surface morphology observation revealed that the shape of chitosan nanoparticles were like lumps with imperfect round shape within micrometer-size. Antioxidant activity assay showed that the F1 had the highest antioxidant activity compared to F0, F2 and F3. 

scholarly journals Sea grapes powder with addition of tempe rich in collagen: An anti-aging functional food

F1000Research ◽  
2021 ◽  
Vol 10 ◽  
pp. 789
Author(s):  
Happy Kurnia Permatasari ◽  
Fahrul Nurkolis ◽  
Christopherous Diva Vivo ◽  
Sutamara Lasurdi Noor ◽  
Rahmawati Rahmawati ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Particle Size ◽  
Functional Food ◽  
Functional Foods ◽  
Inhibition Activity ◽  
Aging Effects ◽  
Freeze Dried ◽  
Aging Properties ◽  
Tyrosinase Inhibitors ◽  
Collagen Powder

Background: This study aimed to determine the potential anti-aging effects of sea grapes and tempe (Fermented soybeans) collagen particle size, by measuring the activities of anti-glycation, antioxidant and tyrosinase inhibitors. Methods: Collagen was isolated from sea grapes and tempe freeze dried powder and treated with different NaOH concentrations (0.10 M; 0.20 M; 0.30 M), and CH3COOH 1 M solution, separately. The collagen particle size was adjusted by stirring at 1000 rpm for 5 and 10 hours. 2,2-diphenyl-1-picrylhydrazyl (DPPH) was used to measure the antioxidant activity, and L-tyrosine and L-DOPA (l-3,4-dihydroxyphenylalanine) were used as a marker of tyrosine inhibition.  Results:  The collagen treated with 0.10 M NaOH produced the highest collagen yield (11.65%), and largest particle size (2455 nm). Additionally, this collagen, when treated for 5 hours, exhibited 24.70% antioxidant activity, 62.60% anti-glycation, 8.97% L-tyrosine, and 26.77% L-Dopa inhibition activities. Meanwhile, the collagen treated for 10 hours had a 9.98% antioxidant activity, 41.48% anti-glycation, 7.89% L-tyrosine, and 2.67% L-Dopa inhibition activity.  Conclusion: Sea grapes and tempe collagen powder treated with 0.10 M NaOH and stirred for 5 hours, as functional foods have anti-aging properties.

scholarly journals Preparation and Characterization of Cinnamomum Essential Oil–Chitosan Nanocomposites: Physical, Structural, and Antioxidant Activities

Processes ◽  
2020 ◽  
Vol 8 (7) ◽  
pp. 834 ◽  
Author(s):  
Hongxia Su ◽  
Chongxing Huang ◽  
Ying Liu ◽  
Song Kong ◽  
Jian Wang ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Essential Oil ◽  
Antioxidant Activities ◽  
Chitosan Nanoparticles ◽  
Weight Ratio ◽  
Size Analysis ◽  
X Ray Diffraction ◽  
X Ray ◽  
Oil In Water

In this study, different amounts of cinnamomum essential oil (CEO) were encapsulated in chitosan nanoparticles (NPs) (CS-NPs) through oil-in-water emulsification and ionic gelation. An ultraviolet-visible spectrophotometer, Fourier-transform infrared spectroscopy, synchronous thermal analysis, and X-ray diffraction were employed to analyze the CEO encapsulation. As observed by field-emission scanning electron microscopy, NP size analysis and zeta potential, the prepared CS-NPs, containing CEO (CS-CEO), were spherical with uniformly distributed sizes (diameters: 190–340 nm). The ranges of encapsulation efficiency (EE) and loading capacity (LC) were 4.6–32.9% and 0.9–10.4%, with variations in the starting weight ratio of CEO to CS from 0.11 to 0.53 (w/w). It was also found that the antioxidant activity of the CS-NPs loaded with CEO increased as the EE increased. The active ingredients of the CEO were prevented from being volatilized, significantly improving the chemical stability. The antioxidant activity of CS-CEO was higher than that of the free CEO. These results indicate the promising potential of CS-CEO as an antioxidant for food processing, and packaging applications.

Structural and Morphological Studies of TiO2 Films for Dye-Sensitized Solar Cell Applications

2009 ◽  
Vol 293 ◽  
pp. 63-66
Author(s):  
Abdullah Huda ◽  
Ari Sulistyo Rini ◽  
Mohd. Zulfa Ya ◽  
Mohd Ambar Yarmo
Keyword(s):  
Particle Size ◽  
Self Assembly ◽  
X Ray Diffraction ◽  
Titania Film ◽  
Sem Images ◽  
X Ray ◽  
Dye Sensitized ◽  
Morphological Studies ◽  
Assembly Method ◽  
Evaporation Induced Self Assembly

Titanium dioxide has been successfully synthesized via an evaporation-induced self-assembly method. Here, TiCl4 was chosen as a precursor and CTAB as a surfactant. The SEM images revealed that hydrochloric acid, added during reaction, obviously enlarges the particle size of TiO2 since a larger size was obtained. The X-ray diffraction (XRD) results confirmed the appearance of both the anatase and rutile structures of TiO2 after titania film was calcined at 450°C for 1 hour. The TEM results showed that the evaporation temperature had no significant impact upon the particle size of TiO2.

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