scholarly journals Lamiophlomis rotata Identification via ITS2 Barcode and Quality Evaluation by UPLC-QTOF-MS Couple with Multivariate Analyses

Molecules ◽  
2018 ◽  
Vol 23 (12) ◽  
pp. 3289 ◽  
Author(s):  
Wang Jian ◽  
Gao YunLing ◽  
Chen YiLong ◽  
Chen YiWen ◽  
Zhang Yi ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Performance ◽  
Multivariate Analyses ◽  
Dna Barcodes ◽  
Multivariate Statistical ◽  
Flight Mass Spectrometry ◽  
Chemical Profiles ◽  
Nj Tree ◽  
First Time

Lamiophlomis rotata (L. rotata), is known as “Daba” in the Tibetan region, Ajuga ovalifolia and Oreosolen wartii have also been utilized as substitutes for “Daba”, however, only L. rotata has been officially listed in the Chinese Pharmacopoeia for hemostasis preparations. To safely apply the traditional uses of the herb, internal transcribed spacer 2 (ITS2) DNA barcodes were employed to discriminate L. rotata from its adulterants. For further evaluation of the quality of different originating habitats, the chemical profiles of 25 samples were determined by ultra-high-performance liquid chromatography coupled with time-of-flight mass spectrometry (UPLC-QTOF-MS) coupled with multivariate analyses. ITS2 DNA barcodes differentiated L. rotata from O. wartii and A. ovalifolia accurately. A neighbor-joining (NJ) tree showed that three origins clustered into three clades. Forty-nine compounds were identified in the total ion current (TIC) profile of L. rotata. Additionally, two pairs of isomers were identified for the first time by using mass spectrometry fragmentation. The differences between the variable habitats were determined by multivariate statistical analysis of the UPLC-QTOF-MS data from 25 specimens. Ten compounds were identified as the characteristic markers distinguishing the sample from four geographical origins. The results also suggest that samples from Qinghai and Sichuan province would be the most suitable choice for traditional prescriptions and preparations.

scholarly journals Rapid Screening and Structural Characterization of Antioxidants from the Extract ofSelaginella doederleiniiHieron with DPPH-UPLC-Q-TOF/MS Method

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Gang Wang ◽  
Shun Yao ◽  
Xiu-Xiu Zhang ◽  
Hang Song
Keyword(s):  
Mass Spectrometry ◽  
High Performance ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Rapid Screening ◽  
Flight Mass Spectrometry ◽  
First Time ◽  
Strong Capacity ◽  
Tof Ms

2,2-Diphenyl-1-picrylhydrazyl-ultra-high performance liquid chromatography-Q-time-of-flight mass spectrometry (DPPH-UPLC-Q-TOF/MS), as a rapid and efficient means, now was used for the first time to screen antioxidants fromSelaginella doederleinii. The nine biflavone compounds were screened as potential antioxidants. The biflavones were structurally identified and divided into the three types, that is, amentoflavone-type, robustaflavone-type, and hinokiflavone-type biflavonoids. Among the compounds bilobetin (3) and putraflavone (8) were found fromSelaginella doederleiniifor the first time and others including amentoflavone (1), robustaflavone (2), 4′-methoxy robustaflavone (4), podocarpusflavone A (5), hinokiflavone (6), ginkgetin (7), and heveaflavone (9) were identified previously in the plant. Moreover, nine biflavones possessed a good antioxidant activity via their DPPH free radical scavenging. It demonstrates that DPPH-UPLC-Q-TOF/MS exhibits strong capacity in separation and identification for small molecule. The method is suitable for rapid screening of antioxidants without the need for complicated systems and additional instruments.

scholarly journals Identification of Chemical Markers for the Discrimination of Radix Angelica sinensis Grown in Geoherb and Non-Geoherb Regions Using UHPLC-QTOF-MS/MS Based Metabolomics

Molecules ◽  
2019 ◽  
Vol 24 (19) ◽  
pp. 3536 ◽  
Author(s):  
Kaixue Zhang ◽  
Menglin Yan ◽  
Shu Han ◽  
Longfei Cong ◽  
Liyao Wang ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Mass Spectrometry ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Angelica Sinensis ◽  
Chemical Markers ◽  
Flight Mass Spectrometry ◽  
Senkyunolide I

This research aimed to discover chemical markers for discriminating radix Angelica sinensis (RAS) from different regions and to explore the differences of RAS in the content of four active compounds and anti-inflammatory activities on lipopolysacchride (LPS)-induced RAW264.7 cells and calcium antagonists on the HEK 293T cells of RAS. Nine compounds were selected as characteristic chemical markers by ultra-high-performance liquid chromatography-quadrupole/time-of-flight mass spectrometry (UHPLC-QTOF-MS/MS), based on metabolomics, in order to rapidly discriminate RAS from geoherb and non-geoherb regions. The contents of senkyunolide I and butylidenephthalide in geoherb samples were higher than those in non-geoherb samples, but the contents of ferulic acid and levistolide A were lower in the geoherb samples. Furthermore, the geoherbs showed better nitric oxide (NO) inhibitory and calcium antagonistic activities than the non-geoherbs. These results demonstrate the diversity in quality of RAS between geoherbs and non-geoherbs.

scholarly journals Metabolomics insights into diabetes nephropathy and protective effects of Radix Scutellariae on rats using ultra-high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry

RSC Advances ◽  
2017 ◽  
Vol 7 (27) ◽  
pp. 16494-16504 ◽  
Author(s):  
Lihui Men ◽  
Zifeng Pi ◽  
Yuan Zhou ◽  
Yuanyuan Liu ◽  
Mengying Wei ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Protective Effects ◽  
Multivariate Statistical ◽  
Flight Mass Spectrometry ◽  
Radix Scutellariae ◽  
Diabetes Nephropathy ◽  
Tof Ms

UHPLC-Q-TOF-MS based metabolomics combined with multivariate statistical analysis for evaluating protective effects ofRadix Scutellariaeon DN rats.

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