scholarly journals Application of Real-Time Cell Electronic Analysis System in Modern Pharmaceutical Evaluation and Analysis

Molecules ◽  
2018 ◽  
Vol 23 (12) ◽  
pp. 3280 ◽  
Author(s):  
Guojun Yan ◽  
Qian Du ◽  
Xuchao Wei ◽  
Jackelyn Miozzi ◽  
Chen Kang ◽  
...  
Keyword(s):  
Chinese Medicine ◽  
Herbal Medicine ◽  
Real Time ◽  
Cell Analysis ◽  
Label Free ◽  
Technology Application ◽  
Chinese Herbal ◽  
Analysis System ◽  
Free Dynamic ◽  
Application Prospects

Objective: We summarized the progress of the xCELLigence real-time cell analysis (RTCA) technology application in recent years for the sake of enriching and developing the application of RTCA in the field of Chinese medicine. Background: The RTCA system is an established electronic cellular biosensor. This system uses micro-electronic biosensor technology that is confirmed for real-time, label-free, dynamic and non-offensive monitoring of cell viability, migration, growth, spreading, and proliferation. Methods: We summarized the relevant experiments and literature of RTCA technology from the principles, characteristics, applications, especially from the latest application progress. Results and conclusion: RTCA is attracting more and more attention. Now it plays an important role in drug screening, toxicology, Chinese herbal medicine and so on. It has wide application prospects in the area of modern pharmaceutical evaluation and analysis.

Acupuncture plus Herbal Medicine for Alzheimer’s Disease: A Systematic Review and Meta-Analysis

2017 ◽  
Vol 45 (07) ◽  
pp. 1327-1344 ◽  
Author(s):  
Simin Zhou ◽  
Lanlan Dong ◽  
Yuan He ◽  
Hong Xiao
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Chinese Medicine ◽  
Herbal Medicine ◽  
Traditional Chinese Medicine ◽  
Chinese Herbal Medicine ◽  
Meta Analysis ◽  
World Population ◽  
Chinese Herbal ◽  
Significant Difference

Alzheimer’s disease (AD) is associated with the unprecedented aging tendency in our world population and has become a significant health issue. The use of Traditional Chinese Medicine to treat AD has been increasing in recent years. The objective of this meta-analysis is to evaluate the effectiveness of combining acupuncture with herbal medicine to treat AD. Randomized controlled trials (RCTs) of acupuncture plus herbals versus treatment with western drugs for AD were retrieved from 11 databases. The data were extracted by two authors; dichotomous data were expressed as odds ratio (ORs) and 95% confidence intervals (CIs), while continuous data were calculated by mean differences (MDs) with 95% CIs. Although the combined analysis of the score of Activity of Daily Life (ADL) scale MD was [Formula: see text]3.59 (95% CI [Formula: see text]7.18–0.01, [Formula: see text]), which indicates there was no statistically significant difference between the two treatments at reducing the ADL scale score, the pooled results of 12 trials indicated that acupuncture plus Chinese herbal medicine was better than western drugs at improving the effectiveness rate (OR 2.24, 95% CI 1.40–3.56), the combined evidence of 11 articles showed that acupuncture plus Chinese herbal medicine was more effective than western drugs at improving the scores for the Mini Mental State Examination (MMSE) scale (2.10, 95% CI 0.69–3.51, [Formula: see text]) and the traditional Chinese medicine symptom (MD 5.07, 95% CI 3.90–6.25, [Formula: see text]). From the current research results, acupuncture plus herbal medicine may have advantages over western drugs for treating AD. Nevertheless, well-designed RCTs with a larger sample size are required in the future.

scholarly journals iCELLigence real-time cell analysis system for examining the cytotoxicity of drugs to cancer cell lines

2017 ◽  
Vol 14 (3) ◽  
pp. 1866-1870 ◽  
Author(s):  
Leyla Türker Şener ◽  
Gürcan Albeni̇z ◽  
Bi̇rcan Di̇nç ◽  
Işil Albeni̇z
Keyword(s):  
Real Time ◽  
Cell Lines ◽  
Cancer Cell ◽  
Cancer Cell Lines ◽  
Cell Analysis ◽  
Analysis System

scholarly journals Chinese Herbal Medicine for Treating Epilepsy

2021 ◽  
Vol 15 ◽  
Author(s):  
Chia-Hui Lin ◽  
Ching-Liang Hsieh
Keyword(s):  
Chinese Medicine ◽  
Herbal Medicine ◽  
Chinese Herbal Medicine ◽  
Chinese Herb ◽  
Diet Therapy ◽  
Cochrane Library ◽  
Randomized Controlled Clinical Trials ◽  
Chinese Herbal ◽  
Alternative Medicines ◽  
History Of Use

Chinese herbal medicine has a long history of use for treating epilepsy. Because of the side effects of Western antiepileptic therapy and the quest for more accessible treatment, complementary and alternative medicines have become popular. Traditional Chinese medical diet therapy appears to be safe and effective. We searched PubMed and the Cochrane Library through November 2020 for the use of traditional Chinese medicine in clinical settings, including plants, fungi, and animals. Combinations of keywords included “epilepsy,” “seizure,” “antiepileptic,” “anticonvulsive,” “Chinese herbal medicine,” “Chinese herb,” and each of the Latin names, English names, and scientific names of herbs. We also summarized the sources and functions of these herbs in Chinese medicine. Different herbs can be combined to increase antiepileptic effects through various mechanisms, including anti-inflammation, antioxidation, GABAergic effect enhancement, modulation of NMDA channels and sodium channel, and neuroprotection. Despite reports of their anticonvulsive effects, adequate experimental evidence and randomized controlled clinical trials are required to confirm their antiepileptic effects.

scholarly journals A Randomized, Controlled Clinical Trial of Combining Therapy with Traditional Chinese Medicine-Based Psychotherapy and Chinese Herbal Medicine for Menopausal Women with Moderate to Serious Mood Disorder

2019 ◽  
Vol 2019 ◽  
pp. 1-13 ◽  
Author(s):  
Yuyan Zeng ◽  
Xuchun Huang ◽  
Changqian Chen ◽  
Guangning Nie ◽  
Xiaojing Cao ◽  
...  
Keyword(s):  
Chinese Medicine ◽  
Herbal Medicine ◽  
Traditional Chinese Medicine ◽  
Mood Disorder ◽  
Chinese Herbal Medicine ◽  
Chinese Herbal ◽  
Self Rating ◽  
Menopausal Women ◽  
Significant Difference

Objective. To comparatively examine the effectiveness and safety of the combination therapy of traditional Chinese medicine formula Bushen-Shugan granule and psychotherapy (BSSG-P) and Chinese herbal medicine Bushen-Shugan granule (BSSG) alone in the treatment of moderate to serious mood disorder in menopausal women. Methods. In our previous clinical studies, BSSG-P had been proved to be superior to BSSG, psychological treatment, and placebo in improving mild mood disorder in menopausal women. In this study, we analyzed the efficacy of BSSG-P and BSSG in the treatment of moderate to serious mood disorder. Eighty-five eligible participants, who were diagnosed as menopausal women with moderate to serious mood disorder and categorized as kidney deficiency and liver-qi stagnation pattern, were randomly assigned into two groups and treated with BSSG-P or BSSG. They were subjected to an 8-week treatment period and a 4-week follow-up study. The primary outcome instrument was the Greene Climacteric Scale, Self-Rating Depression Scale (SDS), and Self-Rating Anxiety Scale (SAS), respectively. Results. When comparing all time points with baseline, both BSSG-P and BSSG markedly decreased the total score of Greene, SDS, and SAS and the score of each dimension, in which BSSG-P exerted superior effect after 8-week treatment and 4-week follow-up (P<0.05). Furthermore, BSSG-P also showed great advantage in reducing the score of Greene, SDS, and SAS for menopausal women with moderate mood disorder at the end of the 8th and 12th week when compared with BSSG (P < 0.05), whereas there was no significant difference between groups at any time point for patients with serious mood disorder (P>0.05). No serious event occurred in both groups, and no significant difference was found between groups in adverse event proportion. Conclusions. BSSG-P was superior to BSSG in improving the physical and psychological symptoms of menopausal women with mood disorder. For patients with moderate mood disorder, BSSG-P showed obvious advantages; however, no superiority was observed for serious mood disorder.

The (pro)renin receptor and soluble (pro)renin receptor in choriocarcinoma

Reproduction ◽  
2021 ◽  
Author(s):  
Saije K Morosin ◽  
Sarah Jane Delforce ◽  
Richard G. S. Kahl ◽  
Celine Corbisier de Meaultsart ◽  
Eugenie R Lumbers ◽  
...  
Keyword(s):  
Real Time ◽  
Cell Invasion ◽  
Cell Analysis ◽  
Invasive Potential ◽  
Cellular Invasion ◽  
Protein Levels ◽  
Hcg Secretion ◽  
Cytotrophoblast Cells ◽  
Choriocarcinoma Cells ◽  
Analysis System

This study aimed to determine if the (pro)renin receptor (ATP6AP2) changes the cellular profile of choriocarcinomas from cytotrophoblast cells to terminally syncytialised cells and ascertain whether this impacts on the invasive potential of choriocarcinoma cells. Additionally, we aimed to confirm that FURIN and/or site 1 protease (MBTPS1) cleave soluble ATP6AP2 (sATP6AP2) in BeWo choriocarcinoma cells and determine whether sATP6AP2 levels reflect the cellular profile of choriocarcinomas. BeWo choriocarcinoma cells were treated with ATP6AP2 siRNA, FURIN siRNA, DEC-RVKR-CMK (to inhibit FURIN activity) or PF 429242 (to inhibit MBTPS1 activity). Cells were also treated with forskolin, to induce syncytialisation, or vehicle and incubated for 48h before collection of cells and supernatants. Syncytialisation was assessed by measuring hCG secretion (by ELISA) and E-cadherin protein levels (by immunoblot and immunocytochemistry). Cellular invasion was measured using the xCELLigence real-time cell analysis system and secreted sATP6AP2 levels measured by ELISA. Forskolin successfully induced syncytialisation and significantly increased both BeWo choriocarcinoma cell invasion (P<0.0001) and sATP6AP2 levels (P=0.02). Treatment with ATP6AP2 siRNA significantly inhibited syncytialisation (decreased hCG secretion (P=0.005), the percent of nuclei in syncytia (P=0.05)), forskolin-induced invasion (P=0.046) and sATP6AP2 levels (P<0.0001). FURIN siRNA and DEC-RVKR-CMK significantly decreased sATP6AP2 levels (both P<0.0001). In conclusion, ATP6AP2 is important for syncytialisation of choriocarcinoma cells and thereby limits choriocarcinoma cell invasion. We postulate that sATP6AP2 could be used as a biomarker measuring the invasive potential of choriocarcinomas. Additionally, we confirmed that FURIN, not MBTPS1, cleaves sATP6AP2 in BeWo cells, but other proteases (inhibited by DEC-RVKR-CMK) may also be involved.

scholarly journals Single Cell Analysis: Label-Free Optofluidic Nanobiosensor Enables Real-Time Analysis of Single-Cell Cytokine Secretion (Small 26/2018)

Small ◽  
2018 ◽  
Vol 14 (26) ◽  
pp. 1870119 ◽  
Author(s):  
Xiaokang Li ◽  
Maria Soler ◽  
Crispin Szydzik ◽  
Khashayar Khoshmanesh ◽  
Julien Schmidt ◽  
...  
Keyword(s):  
Single Cell ◽  
Real Time ◽  
Single Cell Analysis ◽  
Cytokine Secretion ◽  
Cell Analysis ◽  
Label Free ◽  
Time Analysis ◽  
Real Time Analysis
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