scholarly journals A Label-Free Fluorescent DNA Calculator Based on Gold Nanoparticles for Sensitive Detection of ATP

Molecules ◽  
2018 ◽  
Vol 23 (10) ◽  
pp. 2494 ◽  
Author(s):  
Jingjing Zhang ◽  
Shizhi Zhang ◽  
Chaoqun Niu ◽  
Chen Liu ◽  
Jie Du ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Deoxyribonucleic Acid ◽  
Logic Gate ◽  
Sensitive Detection ◽  
Label Free ◽  
Detection Range ◽  
Highly Sensitive ◽  
Time Logic ◽  
Fluorescence Dye

Herein we described a deoxyribonucleic acid (DNA) calculator for sensitive detection of the determination of adenosine triphosphate (ATP) using gold nanoparticles (GNP) and PicoGreen fluorescence dye as signal transducer, and ATP and single-stranded DNA (DNA-M′) as activators. The calculator-related performances including linearity, reaction time, logic gate, and selectivity were investigated, respectively. The results revealed that this oligonucleotide sensor was highly sensitive and selective. The detection range was 50–500 nmol/L (R2 = 0.99391) and the detection limit was 46.5 nmol/L. The AND DNA calculator was successfully used for the ATP detection in human urine. Compared with other methods, this DNA calculator has the characteristics of being label-free, non-enzymic, simple, and highly sensitive.

scholarly journals A Label-Free Fluorescent AND Logic Gate Aptasensor for Sensitive ATP Detection

Sensors ◽  
2018 ◽  
Vol 18 (10) ◽  
pp. 3281 ◽  
Author(s):  
Jingjing Zhang ◽  
Chunzheng Yang ◽  
Chaoqun Niu ◽  
Chen Liu ◽  
Xuepin Cai ◽  
...  
Keyword(s):  
Deoxyribonucleic Acid ◽  
Limit Of Detection ◽  
Low Cost ◽  
Logic Gate ◽  
Label Free ◽  
Detection Range ◽  
Sensitivity Range ◽  
Highly Sensitive ◽  
Input Signals ◽  
Atp Detection

In this study, a label-free fluorescent, enzyme-free, simple, highly sensitive AND logic gate aptasensor was developed for the detection of adenosine triphosphate (ATP). Double-stranded deoxyribonucleic acid (DNA) with cohesive ends was attached to graphene oxide (GO) to form an aptasensor probe. ATP and single-stranded DNA were used as input signals. Fluorescence intensity of PicoGreen dye was used as an output signal. The biosensor-related performances, including the logic gate construction, reaction time, linearity, sensitivity, and specificity, were investigated and the results showed that an AND logic gate was successfully constructed. The ATP detection range was found to be 20 to 400 nM (R2 = 0.9943) with limit of detection (LOD) of 142.6 pM, and the sensitivity range was 1.846 × 106 to 2.988 × 106 M−1. This method for the detection of ATP has the characteristics of being simple, low cost, and highly sensitive.

scholarly journals A label-free protamine-assisted colorimetric sensor for highly sensitive detection of S1 nuclease activity

The Analyst ◽  
2020 ◽  
Vol 145 (7) ◽  
pp. 2774-2778
Author(s):  
Jiahui Wen ◽  
Yongming Liu ◽  
Jingwen Li ◽  
Hao Lin ◽  
Yiran Zheng ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Colorimetric Method ◽  
Nuclease Activity ◽  
Colorimetric Sensor ◽  
Sensitive Detection ◽  
Label Free ◽  
S1 Nuclease ◽  
Highly Sensitive ◽  
Highly Sensitive Detection

A label-free, simple and rapid colorimetric method for the sensitive detection of S1 nuclease activity based on protamine-assisted aggregation of gold nanoparticles.

Label-free and highly sensitive detection of DNA adenine methylation methyltransferase through cathodic photoelectrochemistry

The Analyst ◽  
2021 ◽  
Author(s):  
Fang Li ◽  
Xiu-Ming Wu ◽  
Mengmeng Gu ◽  
Guang-Li Wang
Keyword(s):  
Potassium Ferricyanide ◽  
Sensitive Detection ◽  
Label Free ◽  
Sensing System ◽  
Highly Sensitive ◽  
Adenine Methylation ◽  
Highly Sensitive Detection

In this work, we report the first exploration of cathodic photoelectrochemistry for the determination of the activity of DNA adenine methylation (Dam) methyltransferase (MTase). In this sensing system, potassium ferricyanide...

A label-free aptasensor for highly sensitive detection of homocysteine based on gold nanoparticles

2020 ◽  
Vol 134 ◽  
pp. 107497 ◽  
Author(s):  
Hadi Beitollahi ◽  
Reza Zaimbashi ◽  
Masoud Torkzadeh Mahani ◽  
Somayeh Tajik
Keyword(s):  
Gold Nanoparticles ◽  
Sensitive Detection ◽  
Label Free ◽  
Highly Sensitive ◽  
Highly Sensitive Detection

A colorimetric Boolean INHIBIT logic gate for the determination of sulfide based on citrate-capped gold nanoparticles

RSC Advances ◽  
2015 ◽  
Vol 5 (72) ◽  
pp. 58574-58579 ◽  
Author(s):  
Hao-Hua Deng ◽  
Gang-Wei Wu ◽  
Xiao-Qing Lin ◽  
Xiong-Wei Xu ◽  
Ai-Lin Liu ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Logic Gate

An INHIBIT logic gate is designed based on citrate-capped gold nanoparticles and successfully utilized to the determination of sulfide.

Highly sensitive detection of copper(i) and copper(ii) in cell specimens by CE-UV with large-volume sample stacking

2016 ◽  
Vol 8 (21) ◽  
pp. 4272-4276 ◽  
Author(s):  
Cheng Chen ◽  
Xin Li ◽  
Xia Xie ◽  
Fengxia Chang ◽  
Meixian Li ◽  
...  
Keyword(s):  
Large Volume ◽  
Sensitive Detection ◽  
Sample Stacking ◽  
Highly Sensitive ◽  
Simultaneous Separation ◽  
Sensitive Determination ◽  
Highly Sensitive Detection ◽  
Determination Of Copper ◽  
Large Volume Sample Stacking

A CE-UV method has been developed for the simultaneous separation and sensitive determination of copper(i) and copper(ii) in cell specimens.

Redox-induced target-dependent ratiometric fluorescence sensing strategy and logic gate operation for detection of α-glucosidase activity and its inhibitor

2021 ◽  
Author(s):  
Xucan Yuan ◽  
Yi Sun ◽  
Pengfei Zhao ◽  
Longshan Zhao ◽  
Zhili Xiong
Keyword(s):  
Redox Reaction ◽  
Logic Gate ◽  
Sensitive Detection ◽  
Ratiometric Fluorescence ◽  
Fluorescence Sensing ◽  
Gate Operation ◽  
Glucosidase Activity ◽  
Highly Sensitive ◽  
Highly Sensitive Detection ◽  
Ratiometric Fluorescence Sensing

A target-dependent ratiometric fluorescence sensing strategy was designed and fabricated based on redox reaction for highly sensitive detection of α-glucosidase (α-Glu) activity and its inhibitor. In this study, silicon quantum...

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