scholarly journals Microfluidic-Based Single-Cell Study: Current Status and Future Perspective

Molecules ◽  
2018 ◽  
Vol 23 (9) ◽  
pp. 2347 ◽  
Author(s):  
Haiwa Wu ◽  
Jing Zhu ◽  
Yao Huang ◽  
Daming Wu ◽  
Jingyao Sun
Keyword(s):  
Single Cell ◽  
Soft Lithography ◽  
High Efficiency ◽  
Low Cost ◽  
Cell Manipulation ◽  
Current Status ◽  
Future Perspective ◽  
Cellular Processes ◽  
Cell Groups ◽  
Cell Study

Investigation of cell behavior under different environments and manual operations can give information in specific cellular processes. Among all cell-based analysis, single-cell study occupies a peculiar position, while it can avoid the interaction effect within cell groups and provide more precise information. Microfluidic devices have played an increasingly important role in the field of single-cell study owing to their advantages: high efficiency, easy operation, and low cost. In this review, the applications of polymer-based microfluidics on cell manipulation, cell treatment, and cell analysis at single-cell level are detailed summarized. Moreover, three mainly types of manufacturing methods, i.e., replication, photodefining, and soft lithography methods for polymer-based microfluidics are also discussed.

scholarly journals The Possibility of a High-efficiency Wave Power Generation System using Dielectric Elastomers

2021 ◽  
Author(s):  
Kazuhiro Ohyama ◽  
Seiki Chiba ◽  
Mikio Waki ◽  
Changqing Jiang ◽  
Makoto Takeshita ◽  
...  
Keyword(s):  
Power Generation ◽  
Environmental Sustainability ◽  
High Efficiency ◽  
Low Cost ◽  
Artificial Muscle ◽  
Current Status ◽  
Oscillating Water Column ◽  
Generation System ◽  
High Efficient ◽  
Power Generation System

Abstract: Power generation using dielectric elastomer (DE) artificial muscle is attracting attention because of its light weight, low cost, and high efficiency. Since this method is a system that produces electricity without emitting carbon dioxide nor using rare earths, it would contribute to the goal of environmental sustainability. In this paper, the background of DEs, the associated high-efficient wave energy generation (WEG) systems that we developed using DEs, as well as the latest development of its material are summarized. By covering the challenges we face and the achievements that we’ve reached, we can discuss the opportunities to build the foundation of a recycled energy society through the usage of these WEGs. On the other hand, to make these possibilities commercially successful, the advantages of DEs need to be integrated with traditional technologies. To achieve this, we also consider the method of using DEs alone and a system used in combination with an oscillating water column. Finally, the current status and future of DEGs are discussed.

scholarly journals Triacontanol Promotes the Fruit Development and Retards Fruit Senescence in Strawberry: A Transcriptome Analysis

Plants ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 488
Author(s):  
Qianqian Pang ◽  
Xueqin Chen ◽  
Jinhua Lv ◽  
Teng Li ◽  
Jinggui Fang ◽  
...  
Keyword(s):  
Plant Growth ◽  
Fruit Development ◽  
Growth And Development ◽  
High Efficiency ◽  
Low Cost ◽  
Sugar Content ◽  
Regulation Mechanism ◽  
Cellular Processes ◽  
Go Enrichment ◽  
Related Enzyme

Triacontanol (TA) is a non-toxic, pollution-free, low-cost, high-efficiency, broad-spectrum plant growth regulator that plays an important role in plant growth and development, but its regulation mechanism of strawberry (Sweet charlie, Fragaria × ananassa Duch.) fruit development is still unclear. In this study, we showed that TA treatment (50 μM) could promote fruit development by up-regulating factors related to fruit ripening-related growth and development. TA increased fruit sugar content and anthocyanin accumulation, and many stress-related enzyme activities. In the meantime, Illumina RNA-Seq technology was used to evaluate the effect of TA treatment on strawberry fruit senescence. The results showed that 9338 differentially expressed genes (DEGs) were obtained, including 4520 up-regulated DEGs and 4818 down-regulated DEGs. We performed gene ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis of these DEGs. The results showed that TA treatment caused changes in transcript levels related to cellular processes, hormones and secondary metabolism, such as DNA metabolic processes, flavonoid synthesis, and plant hormone signal transduction. Bioinformatics analysis showed that many transcription factors were related to fruit maturity. Taken together, this study will provide new insights into the mechanism of strawberry development and postharvest response to TA treatment.

Dielectrophoresis Tweezers for Single Cell Manipulation Using Metal Coated Chemically Etched Fiber

2014 ◽  
Vol 625 ◽  
pp. 678-682
Author(s):  
Kozo Taguchi ◽  
Keishu Aritoshi ◽  
Kyohei Nishimoto ◽  
Shun Fukutomi
Keyword(s):  
Single Cell ◽  
Low Cost ◽  
Yeast Cells ◽  
Cell Manipulation ◽  
Signal Frequency ◽  
Experimental Investigations ◽  
Target Cells ◽  
Promising Tool ◽  
Single Target ◽  
Single Cell Manipulation

We proposed a simple and low cost dielectrophoretic device to trap and isolate single target cells. The device consisted of a metal coated chemically etched fiber and an AC signal generator. It did not require microfabrication technologies or sophisticated electronics. Using this system, we could easily trap and isolate yeast cells at will. Furthermore, our dielectrophoretic manipulator also could discriminate between live and dead cells by tuning of the applied signal frequency. From these experimental investigations, it was found that our proposed dielectrophoresis tweezers using metal coated chemically etched fiber was a promising tool for the single cell manipulation and isolation.

High quality diffractive optical elements (DOEs) using SMILE imprint technique

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Simon Drieschner ◽  
Fabian Kloiber ◽  
Marc Hennemeyer ◽  
Jan J. Klein ◽  
Manuel W. Thesen
Keyword(s):  
Soft Lithography ◽  
High Efficiency ◽  
Low Cost ◽  
Industrial Applications ◽  
Diffractive Optical Elements ◽  
Large Area ◽  
High Quality ◽  
Optical Elements ◽  
Uv Curable ◽  
Aspect Ratios

Abstract Augmented reality (AR) enhancing the existing natural environment by overlaying a virtual world is an emerging and growing market and attracts huge commercial interest into optical devices which can be implemented into head-mounted AR equipment. Diffractive optical elements (DOEs) are considered as the most promising candidate to meet the market’s requirements such as compactness, low-cost, and reliability. Hence, they allow building alternatives to large display headsets for virtual reality (VR) by lightweight glasses. Soft lithography replication offers a pathway to the fabrication of large area DOEs with high aspect ratios, multilevel features, and critical dimensions below the diffractive optical limit down to 50 nm also in the scope of mass manufacturing. In combination with tailored UV-curable photopolymers, the fabrication time can be drastically reduced making it very appealing to industrial applications. Here, we illustrate the key features of high efficiency DOEs and how the SMILE (SUSS MicroTec Imprint Lithography Equipment) technique can be used with advanced imprint photopolymers to obtain high quality binary DOEs meeting the market’s requirements providing a very versatile tool to imprint both nano- and microstructures.

scholarly journals Salt-Resistive Photothermal Materials and Microstructures for Interfacial Solar Desalination

2021 ◽  
Vol 9 ◽  
Author(s):  
Xiaoqiang Yu ◽  
Qian Zhang ◽  
Xin Liu ◽  
Ning Xu ◽  
Lin Zhou
Keyword(s):  
Energy Transfer ◽  
High Efficiency ◽  
Low Cost ◽  
High Energy ◽  
Current Status ◽  
Long Term Stability ◽  
Solar Desalination ◽  
Point Of Use ◽  
Art Research

Solar interfacial evaporation, featured by high energy transfer efficiency, low cost, and environmental compatibility, has been widely regarded as a promising technology for solar desalination. However, the interplay between energy transfer and water transport in the same channels suggests that the tradeoff between high efficiency and long-term stability inherently exists in conventional photothermal nanomaterials. We summarize state-of-the-art research on various anti-salt clogging photothermal microstructures as long-term stable interfacial solar evaporators for solar desalination. The review starts with an overview of the current status and the fundamental limit of photothermal materials for solar desalination. Four representative strategies are analyzed in detail with the most recent experimental demonstrations, including fluid convection enhancement, surface wettability engineering, energy-mass-path decoupling, and surface chemistry engineering. Finally, this article focuses on the challenges in anti-salt clogging solar interfacial evaporators and potential point-of-use applications in the future.

scholarly journals OPAM, an Open source, 3D printed Low-cost Micro-Manipulator for Single Cell Manipulation

2021 ◽  
Author(s):  
Jiang Xu ◽  
Zhuowei Du ◽  
Paul Hsi Liu ◽  
Yi Kou ◽  
Lin Chen
Keyword(s):  
Single Cell ◽  
Open Source ◽  
Low Cost ◽  
Cell Manipulation ◽  
Stepper Motor ◽  
Great Promise ◽  
3D Printer ◽  
Cell Capture ◽  
Entry Level ◽  
3D Printed

We introduce OPAM, an Open source, low-cost (under $150), 3D-Printed, stepper motor driven, Arduino based, single cell Micromanipulator (OPAM). Modification of a commercial stepper motor led to dramatically increased stability and maneuverability of the motor, based on which the micromanipulator was designed. All components of this micromanipulator can be 3D printed using an entry-level 3D printer and assembled with ease. With this single cell manipulator, successful targeted single cell capture and transfer was confirmed under the microscope, which showed great promise for single cell related experiments.

Nanoliters Discharge/Suction by Thermoresponsive Polymer Actuated Probe and Applied for Single Cell Manipulation

2010 ◽  
Vol 22 (5) ◽  
pp. 644-650 ◽  
Author(s):  
Masaru Takeuchi ◽  
◽  
Masahiro Nakajima ◽  
Masaru Kojima ◽  
Toshio Fukuda ◽  
...  
Keyword(s):  
Single Cell ◽  
Volume Change ◽  
Target Cell ◽  
Single Cell Analysis ◽  
Low Cost ◽  
Cell Manipulation ◽  
Cell Analysis ◽  
Thermoresponsive Polymer ◽  
Solution Volume ◽  
Single Cell Manipulation

We propose the Thermoresponsive Polymer Actuated (TPA) probe which uses thermoresponsive polymer poly (N-isopropylacrylamide) (PNIPAAm) volume change as an actuator. The proposed probe is applicable to single cell analysis, especially single cell manipulation. The TPA probe can discharge and suck solution in several nanoliters (nl) using the volume change. Normally, it is difficult to realize solution discharge and suction less than several dozen nl by the conventional air- or oil-pressure-actuated probe. We designed the TPA probe for low-cost fabrication and disposable use. The probe also takes in and ejects on a nl order by simply switching a heater on and off. PNIPAAm solution volume change was evaluated in this paper. The manipulation of single microbead and the suction of target cell were also demonstrated by the TPA probe in the semi-closed microchip. It is considered that the TPA probe can contribute to the manipulation of single cell.

Route towards low cost-high efficiency second generation solar cells: current status and perspectives

2014 ◽  
Vol 26 (8) ◽  
pp. 5562-5573 ◽  
Author(s):  
O. Vigil-Galán ◽  
Maykel Courel ◽  
J. A. Andrade-Arvizu ◽  
Y. Sánchez ◽  
M. Espíndola-Rodríguez ◽  
...  
Keyword(s):  
Solar Cells ◽  
Second Generation ◽  
High Efficiency ◽  
Low Cost ◽  
Current Status

scholarly journals The Possibility of a High-Efficiency Wave Power Generation System Using Dielectric Elastomers

Energies ◽  
2021 ◽  
Vol 14 (12) ◽  
pp. 3414
Author(s):  
Seiki Chiba ◽  
Mikio Waki ◽  
Changqing Jiang ◽  
Makoto Takeshita ◽  
Mitsugu Uejima ◽  
...  
Keyword(s):  
Power Generation ◽  
Environmental Sustainability ◽  
High Efficiency ◽  
Low Cost ◽  
Artificial Muscle ◽  
Current Status ◽  
Oscillating Water Column ◽  
Generation System ◽  
Power Generation System ◽  
Recycling Society

Power generation using dielectric elastomer (DE) artificial muscle is attracting attention because of its light weight, low cost, and high-efficiency. Since this method is a system that produces electricity without emitting carbon dioxide nor using rare earths, it would contribute to the goal of environmental sustainability. In this paper, the background of DEs, the associated high efficiency wave energy generation (WEG) systems that we developed using DEs, as well as the latest development of its material are summarized. By covering both the challenges and achievements, this paper discusses the opportunities to build the foundation of an energy recycling society through the usage of these WEGs. To make these possibilities commercially successful, the advantages of DEs need to be integrated with traditional technologies. To achieve this, the method of using DEs alone and a system used in combination with an oscillating water column were also considered. Finally, the current status and future of DE generators (DEGs) are discussed.

scholarly journals A Single-Cell Study of a Highly Effective Hog1 Inhibitor for in Situ Yeast Cell Manipulation

Micromachines ◽  
2014 ◽  
Vol 5 (1) ◽  
pp. 81-96 ◽  
Author(s):  
Charlotte Blomqvist ◽  
Peter Dinér ◽  
Morten Grøtli ◽  
Mattias Goksör ◽  
Caroline Adiels
Keyword(s):  
Yeast Cell ◽  
Single Cell ◽  
Cell Manipulation ◽  
Highly Effective ◽  
Cell Study
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