scholarly journals A Single-Cell Study of a Highly Effective Hog1 Inhibitor for in Situ Yeast Cell Manipulation

Micromachines ◽  
2014 ◽  
Vol 5 (1) ◽  
pp. 81-96 ◽  
Author(s):  
Charlotte Blomqvist ◽  
Peter Dinér ◽  
Morten Grøtli ◽  
Mattias Goksör ◽  
Caroline Adiels
Keyword(s):  
Yeast Cell ◽  
Single Cell ◽  
Cell Manipulation ◽  
Highly Effective ◽  
Cell Study

Simulation of Single Cell Trapping Via Hydrodynamic Manipulation

2014 ◽  
Vol 69 (8) ◽  
Author(s):  
Amelia Ahmad Khalili ◽  
Mohd Ariffanan Mohd Basri ◽  
Mohd Ridzuan Ahmad
Keyword(s):  
Yeast Cell ◽  
Single Cell ◽  
Flow Rate ◽  
Cell Model ◽  
Cell Manipulation ◽  
Hydrodynamic Resistance ◽  
Cell Trapping ◽  
Trapping Model ◽  
Single Cell Trapping ◽  
Suction Flow

Microfluidic devices are important for the single cell analysis such as cell mechanical and electrical characterization. Single cell characterization could be related to many significant applications including early disease diagnosis. However to perform the single cell manipulation, firstly a single cell have to be isolated and a platform for the cell manipulation have to be provided. One of the methods to trap a single cell is by using hydrodynamic trapping in the microfluidic channel. This study provides a finite element model for single cell trapping for a yeast cell model. The objectives of the simulations are to obtain the appropriate channels’ geometry and optimized ratio of the fluid’s inlet and suction flow rate to trap a single yeast cell. Trap channel was designed to trap a 5μm yeast cell with a suction hole placed in the end of the trap channel. Design geometry and the ratio of fluid flow rates for the cell trapping model were studied using the hydrodynamic resistance concept. The analysis was carried out using numerical solutions from the finite element ABAQUS-FEA software. Using the cell trapping model, a single yeast cell able to be trapped into the trap channel with optimized channel’s suction hole’s geometry and appropriate fluid’s inlet and suction flow rate ratio. The appropriate QTrap/QMain ratio to perform cell trapping using hydrodynamic resistance concept is the ratio value above 1. A 5 μm yeast cell model able to be trap inside a trap channel with the height, width and length of 7 μm by manipulating the suction hole’s flow rate of  1.5 and 2.0 μm of height, 7 and 3 μm of length and width, respectively which situated at the centre edge of the trap channel.

scholarly journals In Situ 3D-Printing using a Bio-ink of Protein–photosensitizer Conjugates for Single-cell Manipulation

2020 ◽  
Vol 3 (4) ◽  
pp. 2378-2384 ◽  
Author(s):  
Akihiro Nishiguchi ◽  
Gent Kapiti ◽  
J. Robin Höhner ◽  
Smriti Singh ◽  
Martin Moeller
Keyword(s):  
3D Printing ◽  
Single Cell ◽  
Cell Manipulation ◽  
Single Cell Manipulation

scholarly journals Microfluidic-Based Single-Cell Study: Current Status and Future Perspective

Molecules ◽  
2018 ◽  
Vol 23 (9) ◽  
pp. 2347 ◽  
Author(s):  
Haiwa Wu ◽  
Jing Zhu ◽  
Yao Huang ◽  
Daming Wu ◽  
Jingyao Sun
Keyword(s):  
Single Cell ◽  
Soft Lithography ◽  
High Efficiency ◽  
Low Cost ◽  
Cell Manipulation ◽  
Current Status ◽  
Future Perspective ◽  
Cellular Processes ◽  
Cell Groups ◽  
Cell Study

Investigation of cell behavior under different environments and manual operations can give information in specific cellular processes. Among all cell-based analysis, single-cell study occupies a peculiar position, while it can avoid the interaction effect within cell groups and provide more precise information. Microfluidic devices have played an increasingly important role in the field of single-cell study owing to their advantages: high efficiency, easy operation, and low cost. In this review, the applications of polymer-based microfluidics on cell manipulation, cell treatment, and cell analysis at single-cell level are detailed summarized. Moreover, three mainly types of manufacturing methods, i.e., replication, photodefining, and soft lithography methods for polymer-based microfluidics are also discussed.

In-Situ Dual Beam (FIBSEM) Techniques for Probe Pad Deposition and Dielectric Integrity Inspection in 0.2 μm Technology DRAM Single Cells

1999 ◽  
Author(s):  
Gunnar Zimmermann ◽  
Richard Chapman
Keyword(s):  
Electron Beam ◽  
Single Cell ◽  
Single Cells ◽  
Ion Beam ◽  
Gate Oxide ◽  
Ion Milling ◽  
Dual Beam ◽  
Sem Imaging ◽  
Innovative Techniques

Abstract Dual beam FIBSEM systems invite the use of innovative techniques to localize IC fails both electrically and physically. For electrical localization, we present a quick and reliable in-situ FIBSEM technique to deposit probe pads with very low parasitic leakage (Ipara < 4E-11A at 3V). The probe pads were Pt, deposited with ion beam assistance, on top of highly insulating SiOx, deposited with electron beam assistance. The buried plate (n-Band), p-well, wordline and bitline of a failing and a good 0.2 μm technology DRAM single cell were contacted. Both cells shared the same wordline for direct comparison of cell characteristics. Through this technique we electrically isolated the fail to a single cell by detecting leakage between the polysilicon wordline gate and the cell diffusion. For physical localization, we present a completely in-situ FIBSEM technique that combines ion milling, XeF2 staining and SEM imaging. With this technique, the electrically isolated fail was found to be a hole in the gate oxide at the bad cell.

scholarly journals Microfluidic extraction and stretching of chromosomal DNA from single cell nuclei for DNA fluorescence in situ hybridization

2012 ◽  
Vol 14 (3) ◽  
pp. 443-451 ◽  
Author(s):  
Xiaozhu Wang ◽  
Shin-ichiro Takebayashi ◽  
Evans Bernardin ◽  
David M. Gilbert ◽  
Ravindran Chella ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Single Cell ◽  
Cell Nuclei ◽  
Chromosomal Dna
Sign in / Sign up

Export Citation Format

Share Document