scholarly journals Origanum vulgare ssp. vulgare: Chemical Composition and Biological Studies

Molecules ◽  
2018 ◽  
Vol 23 (8) ◽  
pp. 2077 ◽  
Author(s):  
Ilioara Oniga ◽  
Cristina Pușcaș ◽  
Radu Silaghi-Dumitrescu ◽  
Neli-Kinga Olah ◽  
Bogdan Sevastre ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Salmonella Enteritidis ◽  
Biological Properties ◽  
Gamma Glutamyl Transferase ◽  
Reducing Ability ◽  
Biological Studies ◽  
Antioxidant And Antimicrobial Activity ◽  
Agar Well Diffusion Assay ◽  
New Research ◽  
Glutamyl Transferase

The biological properties and main phenolic compounds of the O. vulgare L. ssp. vulgare extract are described in the present paper. The polyphenolic compounds were analyzed by chromatographic and spectrophotometric techniques. The antioxidant potential was evaluated using several methods: CUPRAC (cupric ion reducing antioxidant capacity), FRAP (ferric reducing ability of plasma), inhibition of lipid peroxidation catalyzed by cytochrome c, and superoxide (SO) scavenging assays. The antimicrobial activity of the oregano extract was evaluated by means of agar-well diffusion assay. The hepatoprotective effect of the O. vulgare extract on CCl4-induced hepatotoxicity was evaluated in rats. Liver injury was estimated by determination of alanine aminotransaminase (ALT), aspartate aminotransaminase (AST), gamma-glutamyl transferase GGT, total protein and albumin concentrations, glutathione peroxidase (GPx), catalase (CAT), superoxide dismutase (SOD) and malondialdehyde (MDA). These values were improved by the administration of oregano extract. A specific phenolic profile was evidenced by these data, with large amounts of rosmarinic and chlorogenic acids. The oregano extract showed very strong antioxidant activity in good agreement with the phenolic content. Antimicrobial activity was good, especially against Salmonella enteritidis and Aspergillus niger strains. The high hepatoprotective, antioxidant and antimicrobial activity, along with polyphenol-rich content, can support the use of O. vulgare in therapy. We also expect our results to open new research directions for designing important new drug products, using indigenous plant material.

scholarly journals Antioxidant and antimicrobial activity of sapodilla (Manilkara zapota L.) fresh, juice and bar

Food Research ◽  
2019 ◽  
pp. 400-406
Author(s):  
Murnisyazwani J. ◽  
Rabeta M.S.
Keyword(s):  
Antimicrobial Activity ◽  
Condensed Tannin ◽  
Total Flavonoid ◽  
Total Flavonoid Content ◽  
Flavonoid Content ◽  
Salmonella Spp ◽  
Antioxidant Power ◽  
Reducing Ability ◽  
Ferric Reducing Antioxidant Power ◽  
Antioxidant And Antimicrobial Activity

This study evaluates the antioxidant and antimicrobial activity of sapodilla fresh, juice and bar using three extraction solvents with different polarities (70% acetone, 80% ethanol and water). For evaluating antioxidant capacity, Ferric Reducing Antioxidant Power (FRAP) and 1,1-Diphenyl-2-Picrylhydrazyl (DPPH) assays were used. The total flavonoid content (TFC) and condensed tannin of the sapodilla juice and bar extracts were determined as well. Antimicrobial activity was measured using Staphylococcus aureus, Salmonella spp., Escherichia coli and Bacillus subtilis. Both sapodilla bar and juice produced more inhibition of the DPPH assay with 70% acetone and 80% ethanol than with the water extraction. The reducing ability of fresh sapodilla was on the order of 70% acetone > 80% ethanol > water. The 70% acetone was the best solvent for yielding condensed tannins. For total flavonoid content, fresh sapodilla and the sapodilla bar and juice showed the highest flavonoid content in 80% ethanol. The results showed that the 70% acetone extract for sapodilla bar and juice exhibited stronger antibacterial activity against gram-positive bacteria. Despite some antioxidants being lost in processed food, these still retained important sources of bioactive compounds. Antimicrobial activity done showed different selectivity for sapodilla juice and bar.

scholarly journals Red Algae as Source of Nutrients with Antioxidant and Antimicrobial Potential

Proceedings ◽  
2020 ◽  
Vol 70 (1) ◽  
pp. 5
Author(s):  
María. Carpena ◽  
Cristina Caleja ◽  
Paula García-Oliveira ◽  
Carla Pereira ◽  
Marina Sokovic ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Organic Acids ◽  
Red Algae ◽  
Antimicrobial Agents ◽  
Biological Properties ◽  
Nutritional Composition ◽  
In Vitro Assays ◽  
Antimicrobial Potential ◽  
Antioxidant And Antimicrobial Activity

Seaweeds have been consumed since ancient times in different cultures, especially in Asian regions. Currently, several scientific studies have highlighted the nutritional value of algae as well as their biological properties. The present work was directed towards the determination of the nutritional composition (ash, protein, fat, carbohydrate content and energy value), the organic acids content, and also the antioxidant and antimicrobial activity of three typical red algae from Galicia: Chondrus crispus, Mastocarpus stellatus and Gigartina pistillata. The nutritional profile was determined by the methods described by AOAC, while organic acids were determined by UPFLC-PDA. The antioxidant activity was assessed using two in vitro assays: TBARS and OxHLIA. At last, antimicrobial activity was tested against two Gram (−), three Gram (+) and three fungus species. The results showed a similar nutritional composition among the three algae, with a low-fat content and a high content of proteins, carbohydrates and energy. Finally, in vitro assays showed significant antioxidant capacity of the three algae and antimicrobial potential against all the tested microorganisms. Thus, the study shows the potential of red algae to be part of the human diet, due to their nutritional content and bioactive properties, acting as antioxidant and antimicrobial agents.

Identification of 3-Bromo-1-Ethyl-1H-Indole as a Potent Anticancer Agent with Promising Inhibitory Effects on GST Isozymes

2020 ◽  
Vol 20 ◽  
Author(s):  
Can Yılmaz ◽  
Sevki Arslan ◽  
Dogukan Mutlu ◽  
Metin Konus ◽  
Abdussamet Kayhan ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
13C Nmr ◽  
Anticancer Agents ◽  
Biological Properties ◽  
Docking Studies ◽  
Pharmaceutical Chemistry ◽  
Live Cells ◽  
1H And 13C Nmr ◽  
Antioxidant And Antimicrobial Activity

Background: Indole based heterocyclic compounds plays important roles in pharmaceutical chemistry due to their unexpected biological and pharmacological properties. Objective: Herein, we describe novel biological properties (antioxidant, antimicrobial and anticancer) of 3-bromo-1-ethyl-1H-indole (BEI) structure. Method: BEI was synthesized from 1-Methyl-2-phenylindole and N-bromosuccinimide and were characterized by using 1H and 13C NMR. 1H and 13C NMR. Cytotoxity was determined by MTT assay. Apoptosis analysis of BEI was determined by Arthur™ imagebased Cytometer. Different methods was applied to assessed the antioxidant activity of BEI. Molecular docking studies were conducted to determine the interactions of bonding between GST isozymes and BEI. Results: According to the antioxidant and antimicrobial activity assays, BEI compound showed less total antioxidant activity compared to trolox standard whereas it showed moderate antimicrobial activity against Aspergillus niger and Phytophora eryhtrospora. Notably, BEI compound demonstrated substantial selective cytotoxicity for the first time towards cancer cell lines and there existed significant decrease in the percentage of live cells treated with BEI, in comparison to the control ones. Interestingly, BEI exhibited a promising glutathione S-transferase isozymes inhibition. Conclusion: The results of this study suggest that BEI seems to be a promising molecule to be used in design of new anticancer agents that provide superiority to present commercial anticancer drugs.

Total phenolic content, flavonoid concentration, antioxidant and antimicrobial activity of methanol extracts from three Seseli L. taxa

2012 ◽  
Vol 7 (6) ◽  
pp. 1116-1122 ◽  
Author(s):  
Jelena Matejić ◽  
Ana Džamić ◽  
Tatjana Mihajilov-Krstev ◽  
Vladimir Ranđelović ◽  
Zoran Krivošej ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Salmonella Enteritidis ◽  
Antimicrobial Agents ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Bacterial Strains ◽  
Methanol Extracts ◽  
Antioxidant And Antimicrobial Activity

AbstractThe present study describes the total phenolic content, concentrations of flavonoids and in vitro antioxidant and antimicrobial activity of methanol extracts from Seseli pallasii Besser, S. libanotis (L.) Koch ssp. libanotis and S. libanotis (L.) Koch ssp. intermedium (Rupr.) P. W. Ball, growing wild in Serbia. The total phenolic content in the extracts was determined using Folin-Ciocalteu reagent and their amounts ranged between 84.04 to 87.52 mg GA (gallic acid)/g. The concentrations of flavonoids in the extracts varied from 4.75 to 19.37 mg Qu (quercetin)/g. Antioxidant activity was analyzed using DPPH reagent. Antioxidant activity ranged from 0.46 to 4.63 IC50 (mg/ml) and from 1.98 to 2.19 mg VitC (vitamin C)/g when tested with the DPPH and ABTS reagents, respectively, using BHA and VitC as controls. The antimicrobial activity of the extracts was investigated using a micro-well dilution assay for the most common human gastrointestinal pathogenic bacterial strains: Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 9027, Salmonella enteritidis ATCC 13076, Bacillus cereus ATCC 10876, Listeria monocytogenes ATCC15313, Staphylococcus aureus ATCC 25923 and Candida albicans ATCC 10231. This finding suggests that Seseli species may be considered as a natural source of antioxidants and antimicrobial agents.

scholarly journals Biological Properties of Bee Bread Collected from Apiaries Located across Greece

Antibiotics ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 555
Author(s):  
Nikos Asoutis Didaras ◽  
Ioannis Kafantaris ◽  
Tilemachos G. Dimitriou ◽  
Chrysanthi Mitsagga ◽  
Katerina Karatasou ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Antibacterial Properties ◽  
Antimicrobial Properties ◽  
Castanea Sativa ◽  
Biological Properties ◽  
Total Phenolic ◽  
Bee Bread ◽  
Different Seasons ◽  
Antioxidant And Antimicrobial Activity

Bee bread is the only fermented product of the beehive. It constitutes the main source of proteins, lipids, vitamins, and macro- and microelements in honeybee nutrition and it exerts antioxidant and antimicrobial properties, though research on these aspects has been limited so far. In this study 18 samples of Greek bee bread, two of which were monofloral, were collected during different seasons from diverse locations such as Crete and Mount Athos and were tested for their bioactivity. Samples were analyzed for their antibacterial properties, antioxidant activity, total phenolic content (TPC), and total flavonoid content (TFC). The antimicrobial activity of each sample was tested against Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumoniae, and Salmonella typhimurium. Our data demonstrate that all samples exert inhibitory and most of them bactericidal activity against at least two pathogens. Furthermore, all samples exert significant antioxidant activity, where the monofloral Castanea Sativa sample demonstrated superior antioxidant activity. Nevertheless, the antioxidant and antimicrobial activity were not strongly correlated. Furthermore, machine learning methods demonstrated that the palynological composition of the samples is a good predictor of their TPC and ABTS activity. This is the first study that focuses on the biological properties of Greek bee bread and demonstrates that bee bread can be considered a functional food and a possible source of novel antimicrobial compounds.

scholarly journals Evaluation of Gold Nanoparticles in Terms of Their Use in Biomedical Applications

2019 ◽  
Vol 7 (10) ◽  
pp. 1694
Author(s):  
Burcu Biterge-Süt ◽  
Elif Canpolat
Keyword(s):  
Antimicrobial Activity ◽  
Gold Nanoparticles ◽  
Physical Properties ◽  
Biomedical Applications ◽  
Salmonella Enteritidis ◽  
Biological Properties ◽  
Disk Diffusion ◽  
Diffusion Method ◽  
Chloroauric Acid ◽  
Disk Diffusion Method

Recently nanotechnology has become an integral part of modern biomedical applications. Accordingly, nanoparticles are considered as promising components for the development of innovative tags, probes, biosensors and carrier molecules for drug delivery. Spherical colloidal gold nanoparticles (AuNPs) are prime candidates to be utilized for these purposes due to their useful physical properties. However, in order for the gold nanoparticles to be used in nanomedicine, their biological properties should be extensively studied as well. Therefore, in this paper we chemically synthesized gold nanoparticles and studied their physical and biological characteristics to determine their potential use in medicine. Gold nanoparticles were synthesized by the reduction of chloroauric acid (HAuCl4) solution with sodium citrate. The physical properties of the AuNPs were determined by UV–vis spectrophotometer and Zetasizer readings. The antimicrobial activity of the newly synthesized gold nanoparticles on Escherichia coli, Salmonella infantis, Salmonella kentucky, Salmonella typhimurium, Salmonella enteritidis, Pseudomonas aeruginosa, Listeria monocytogenes, Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecalis, Bacillus subtilis and Candida albicans were investigated via disk diffusion method. We found that the AuNPs were monodisperse, stable and not prone to aggregation with an average size of 22.12 nm and an emission band at 522 nm. The disk diffusion tests revealed that the gold nanoparticles did not have a significant growth inhibitory effect on the pathogens tested. In conclusion, here we showed the successful synthesis of gold nanoparticles by a safe and non-toxic method. Furthermore, our evaluation of the antimicrobial activity of these nanoparticles suggests that these molecules could be considered as biologically safe molecules for future medical applications.

Antioxidant and antimicrobial activity of willow herb (Epilobium angustifolium L.)

Planta Medica ◽  
2008 ◽  
Vol 74 (09) ◽  
Author(s):  
I Kosalec ◽  
M Zovko ◽  
K Sankovic ◽  
D Kremer ◽  
S Pepeljnjak
Keyword(s):  
Antimicrobial Activity ◽  
Epilobium Angustifolium ◽  
Antioxidant And Antimicrobial Activity

Recovery from LCDD-associated Severe Liver Cholestasis: a Case Report and Literature Review

2016 ◽  
Vol 25 (1) ◽  
pp. 99-103 ◽  
Author(s):  
Benoit Brilland ◽  
Johnny Sayegh ◽  
Anne Croue ◽  
Frank Bridoux ◽  
Jean-François Subra ◽  
...  
Keyword(s):  
Multiple Myeloma ◽  
Light Chain ◽  
Intrahepatic Cholestasis ◽  
Relevant Literature ◽  
Gamma Glutamyl Transferase ◽  
Light Chain Deposition Disease ◽  
Deposition Disease ◽  
Uncommon Presentation ◽  
Light Chain Deposition ◽  
Glutamyl Transferase

Light chain deposition disease (LCDD) is a rare multisystemic disorder associated with plasma cell proliferation. It mainly affects the kidney, but liver and heart involvement may occur, sometimes mimicking the picture of systemic amyloidosis. Liver disease in LCDD is usually asymptomatic and exceptionally manifests with severe cholestatic hepatitis. We report the case of a 66-year-old female with κ-LCDD and cast nephropathy in the setting of symptomatic multiple myeloma who, after a first cycle of bortezomib-dexamethasone chemotherapy, developed severe and rapidly worsening intrahepatic cholestasis secondary to liver κ-light chain deposition. Intrahepatic cholestasis was attributed to LCDD on the basis of the liver histology and exclusion of possible diagnoses. Chemotherapy was maintained and resulted in progressive resolution of cholestasis. We report here an uncommon presentation of LCDD, with prominent liver involvement that fully recovered with bortezomib-based chemotherapy, and briefly review the relevant literature. Abbreviations: AKI: Acute kidney injury; ALP: alkaline phosphatase; ALT: alanine aminotransferase; AST: aspartate aminotransferase; CMV: Cytomegalovirus; EBV: Epstein–Barr virus; GGT: gamma-glutamyl transferase; HSV: Herpes simplex virus; LC: light chain; LCDD: Light chain deposition disease; MIDD: Monoclonal immunoglobulin deposition disease; MM: Multiple myeloma.

Evaluation of Hematological and Biochemical Changes in Dromedary Camel during the Different Stages of Lactation

2020 ◽  
Vol 21 (3) ◽  
pp. 121-124
Author(s):  
Ahmed El-Sayed
Keyword(s):  
Organic Phosphorus ◽  
Hemoglobin Concentration ◽  
Controlled Study ◽  
Mean Corpuscular Hemoglobin ◽  
Dromedary Camel ◽  
Gamma Glutamyl Transferase ◽  
Corpuscular Hemoglobin ◽  
Stage Of Lactation ◽  
Group 2 ◽  
Glutamyl Transferase

Objective: To assess the potential hematobiochemical alterations in healthy dromedary camel during the different stages of lactation. Design: Randomized controlled study. Animals: Fifteen healthy female dromedary camels, with mean body weight of 499.6 kg and mean age of 20 years. Procedures: Camels were categorized into 3 groups' according to their stage of lactation: group 1, early lactation (1-3 months), group 2, mid-lactation (four-6 months) and group3, late lactation (≥ 7 months). Blood samples were collected from every animals for hematological and biochemical evaluation. Results: Total erythrocyte count (TEC), hemoglobin (Hb), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), total leukocytes (TLC), lymphocytes, neutrophils, monocytes, Calcium, glucose, aspartate aminotransferase (AST), alanine transaminase (ALT), gamma glutamyl transferase (GGT) and alkaline phosphatase (ALP) confirmed significant (p < 0.05) variation between different stages of lactation. However, non-notable (p > 0.05) dissimilarity were located in packed cell volume (PCV), mean corpuscular hemoglobin concentration (MCHC), in organic phosphorus (P), magnesium (Mg), cholesterol, total protein (TP), albumen, globulin, blood urea nitrogen (BUN) and creatinine kinase (CK) in the course of different ranges of lactation, Conclusion and clinical relevance: The results of this investigation may be useful as reference guide for dromedary camel to evaluate the metabolic health status at different stages of lactation.

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