scholarly journals Antioxidation and Melanogenesis Inhibition of Various Dendrobium tosaense Extracts

Molecules ◽  
2018 ◽  
Vol 23 (7) ◽  
pp. 1810 ◽  
Author(s):  
Chin-Feng Chan ◽  
Chin-Tung Wu ◽  
Wen-Ying Huang ◽  
Wen-Shin Lin ◽  
Han-Wei Wu ◽  
...  
Keyword(s):  
Free Radical ◽  
Inhibitory Concentration ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Total Phenol Content ◽  
Mushroom Tyrosinase ◽  
Trolox Equivalent ◽  
Application Potential ◽  
Gallic Acid Equivalent

This study investigated the polyphenol content, antioxidant activity, and inhibition ability of mushroom tyrosinase and melanogenesis of Dendrobium tosaense (DT) extract. Ground DT was extracted using deionized water (W) or 50% ethanol (50E) at room temperature (RT) or 50 °C (50T) for 20 min. The 50T + 50E extract exhibited the highest total phenol content 47.0 ± 4.0 mg gallic acid equivalent/g DT extract, the highest level of 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) free-radical scavenging 66.0 ± 3.0 mg Trolox equivalent/g DT extract, and the highest reducing power 12.00 ± 0.50 mg vitamin C equivalent/g DT extract. The RT + W extract had the highest total flavonoid content 110.0 ± 3.0 mg quercetin equivalent/g DT extract. The RT + 50E extract had the lowest half maximal inhibitory concentration 1.30 ± 0.00 mg/mL for 2,2-diphenyl-1-picrylhydrazyl free-radical scavenging, and the lowest half maximal inhibitory concentration 6.40 ± 0.30 mg/mL for mushroom tyrosinase inhibition activity. DT extracts, especially RT + W and 50T + W, exhibited potent inhibitory effects on melanogenesis of B16/F10 cells. These results demonstrated the application potential of DT extract for skincare.

scholarly journals Antioxidant potential of common leafy vegetables in Bangladesh

2015 ◽  
Vol 44 (1) ◽  
pp. 51-57 ◽  
Author(s):  
Sheikh Julfikar Hossain ◽  
M Shahina Sultana ◽  
M Iftekharuzzaman ◽  
S Amir Hossain ◽  
M Abu Taleb
Keyword(s):  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Leafy Vegetables ◽  
Basella Alba ◽  
Total Antioxidant ◽  
Ethanol Extracts ◽  
Gallic Acid Equivalent

Amount of polyphenols, flavonoids, anthocyanins in ethanol extracts and antioxidant activity of both ethanol and lipophilic extracts of common leafy vegetables in Bangladesh were compared. Among the 12 leafy vegetables, Ipomoea aquatica Forssk. showed the highest content of total polyphenols (38.9 mg gallic acid equivalent (GAE)/g extract) and flavonoids (23.2 mg (+)-catechin equivalent (CE)/g extract). Highest anthocyanins content was found in Amaranthus gangeticus L. (1.15 ?mol/g extract) followed by Pisum sativum L. (0.85 ?mol/g extract). Ethanol extracts of Brassica campestris L., Enhydra fluctuans Lour., I. aquatica, Lagenaria siceraria (Mol.) Standl. and P. sativum exhibited high DPPH free radical scavenging activity with IC50 of 104.2, 85.5, 26.9, 125 and 68.5 ?g/ml, respectively whereas for the same lipophilic pentane extracts of Basella alba L. showed the lowest (68.4 ?g/ml) IC50 followed by I. aquatica (70.4 ?g/ml), E. fluctuans (75.7 ?g/ml), L. siceraria (78.3 ?g/ml) and B. campestris (80.6 ?g/ml). I. aquatica also showed the highest NO free radical scavenging followed by B. campestris and B. alba. Highest reducing power (O.D. = 1.7) was observed for I. aquatica followed by P. sativum (O.D. = 1.12), B. alba (O.D. = 0.86) and L. siceraria (O.D. = 0.72) at 400 ?g/ml extract. They also displayed high total antioxidant capacity. Therefore, the top five potential leafy vegetables consist of both hydrophilic and lipophilic antioxidant(s), the order being I. aquatica > B. campestris > B. alba > P. sativum > and L. siceraria. DOI: http://dx.doi.org/10.3329/bjb.v44i1.22723 Bangladesh J. Bot. 44(1): 51-57, 2015 (March)

scholarly journals BIOMEDICAL POTENTIAL OF SELECTED MUSHROOM SPECIES

2020 ◽  
Vol 11 (2) ◽  
Author(s):  
Tatjana Stanojković ◽  
Marijana Kosanić
Keyword(s):  
Free Radical ◽  
Superoxide Anion ◽  
Anion Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Superoxide Anion Radical ◽  
Total Phenol Content ◽  
Scavenging Activity

The aim of this study was to determine neuroprotective, antioxidant, antimicrobial and cytotoxic potential of acetone extracts of Coprinus comatus and Coprinopsis picacea mushrooms. The neuroprotetive activity was tested against acetylcholinesterase enzyme using the Ellman method. Antioxidant activity was evaluated by free radical scavenging ability, superoxide anion radical scavenging activity and reducing power. The total phenol content was examined using Folin-Ciocalteu reagent. The antimicrobial potential was determined by a microdilution method against 12 microorganisms. The cytotoxic activity was tested using MTT method on the Hela, A549 and LS174 cells. Our results indicate that C. comatus expressed a stronger neuroprotective effect (the percentage of inhibition of acetylcholinesterase was within the range 19.66-51.73%) than C. picacea. In antioxidant effect C. comatus had more potent free radical scavenging activity (IC50 = 276.69 μg/mL) and superoxide anion radical scavenging activity (IC50 = 39.40 μg/mL), while reducing power was relatively similar for both species. The total amount of phenols for C. comatus and C. picacea was 50.57 and 50.20 μg PE/mg of dry extracts, respectively. In antimicrobial activity, C. picacea showed a better effect with MIC values from 0.1 to 7.5 mg/mL. Finally, C. picacea expressed stronger cytotoxicity toward A549 and LS174 cells, while C. comatus was more active against Hela cell.

scholarly journals Antiradical and Cytotoxic Activities of Varying Polarity Extracts of the Aerial Part ofEuphorbia hirtaL.

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Shanmugapriya Perumal ◽  
Roziahanim Mahmud ◽  
Suthagar Pillai Piaru ◽  
Wei Cai Lee ◽  
Surash Ramanathan
Keyword(s):  
Linoleic Acid ◽  
Free Radical ◽  
Aerial Part ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Total Phenolic ◽  
Cytotoxic Activities ◽  
Uv Spectrophotometry ◽  
Etoh Extract

Euphorbia hirtais a well-known ethnomedicinal plant with diverse biological activities. The aim of the present study is to investigate the antiradical activities of various solvent extracts of the aerial part ofE. hirtaas well as to determine the possible cytotoxicity of these extracts. The aerial part ofE. hirtawas extracted with different solvent systems in order to increase polarity. The solvents used were hexane, dichloromethane (DCM), ethyl acetate (EA), ethanol (EtOH), and methanol (MeOH). The contents of total phenols and total flavonoids were analyzed by UV spectrophotometry, whereas the potential free radical-scavenging activities of the extracts were evaluated using the stable free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH), theβ-carotene-linoleic acid bleaching system, and reducing power. The EtOH extract exhibited the highest total phenolic content (237.9±2.26 mg GAE/g), and DCM extract scored the highest total flavonoid content (121±0.15 mg CE/g). The MeOH extract showed a potent free radical-scavenging activity as evidenced by low EC50at 42.81 µg/mL. Interestingly, the EtOH extract demonstrated the highest reducing power activity with EC50value of 6.18 µg/mL. Inβ-carotene-linoleic acid assay, oxidation of linoleic acid was effectively inhibited by DCM extract with96.15±0.78%. All the extracts showed no cytotoxic activity against Vero cells.

scholarly journals EVALUATION OF ANTIOXIDANT AND FREE RADICAL SCAVENGING ACTIVITIES OF DIFFERENT SOLVENT EXTRACTS OF LEAVES OF PIPER UMBELLATUM

2017 ◽  
Vol 10 (2) ◽  
pp. 274
Author(s):  
Sethupandian Geetha ◽  
Kokkaiah Irulandi ◽  
Palanichamy Mehalingam
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Ethyl Acetate ◽  
Methanol Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Frap Assay ◽  
Radical Scavenging Properties

Objective: This study was designed to determine the Total phenol, flavonoid content, antioxidant and free radical scavenging properties of different solvent extracts of Piper umbellatum.  Methods: Different solvent extracts evaluated with DPPH radical scavenging activity and Reducing power activity.Results: The presence of phenol and flavonoid showed highly in the methanol extracts than ethyl acetate and acetone extracts. All the extracts have various level of antioxidant activity. Methanol solvent extract have good extraction and show significant antioxidant activity. The effect of reducing power of methanol extract revealed good antioxidant activity compare with other tested extracts.Conclusion: On the basis of the above results we concluded that methanol extract of Piper umbellatum whole plant extracts shows significant antioxidant activity than ethyl acetate and acetone extracts.Keywords: Piper umbellatum, Antioxidant, DPPH, FRAP assay, Polar, Non polar solvents

scholarly journals Research antioxidant activity of chitooligosacchride by UV-VIS absorption spectrocopy

2020 ◽  
Vol 8 (1) ◽  
pp. 75-81
Author(s):  
Bui Van Hoai ◽  
Ngo Dai Nghiep ◽  
Dao An Quang ◽  
Nguyen Thi Nam Phuong
Keyword(s):  
Molecular Weight ◽  
Antioxidant Activity ◽  
Absorption Spectrum ◽  
Free Radical ◽  
Trichoderma Viride ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Degree Of Deacetylation ◽  
Radical Scavenging Ability

Chitosan with 80% degree of deacetylation was hydrolyzed by cellulase of Trichoderma viride to prepare chitooligosaccharides (COSs) by the fractionation of the COSs with ultrafiltration membrane. The antioxidant activities of the COSs were clarified in this study by reducing power and free radical scavenging ability assay by UV-VIS absorption spectrum. The results show that the COS 1 (10,000-5,000 Da), COS 2 (5,000-3,000 Da), COS 3 (3,000-1,000 Da) and COS 4 (less than1,000 Da) segments have antioxidant properties.The antioxidant activitives of the COSs increased with the increment of concentration, and they also depended on molecular weight.

scholarly journals Aktivitas Antioksidan Minuman Daun Beluntas Teh Hitam (Pluchea indica Less-Camelia sinensis)

2018 ◽  
Vol 38 (2) ◽  
pp. 200
Author(s):  
Paini Sri Widyawati ◽  
Tarsisius Dwi Wibawa Budianta ◽  
Yesiana Dwi Wahyu Werdani ◽  
Maria Olivia Halim
Keyword(s):  
Free Radical ◽  
Secondary Metabolites ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Black Tea ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Iron Ion

The research was conducted to explore the potency of pluchea leaves-black tea drink as antioxidant at various proportions. The research used a single factor randomized block design of pluchea leaves and black tea proportions, including 100:0; 75:25; 50:50; 25:75; and 0:100% (w/w). Each of it was repeated five times. The parameters observed in this study were secondary metabolites, total phenolic, total flavonoids, free radical DPPH scavenging activity, and iron reducing power. The data were statistically analyzed using Analysis of Variance (ANOVA) at α = 5%, if the analysis showed a significant effect then it was continued with Duncan's Multiple Range Test (DMRT). Data was stated as mean ± standard deviation. The results showed that the secondary metabolites containing in drink from pluchea leaves and black tea at various proportions were alkaloids, flavonoids, phenolics, saponins, tannins, and cardiac glycosides. The increasing of black tea proportion in samples added the intensity of alkaloids, phenolics, flavonoids, saponins, and cardiac glycosides compounds detected, but the tannins were decreased. These secondary metabolites were correlated with total phenolic content (TPC) and total flavonoid content (TFC). The increasing of black tea proportion in drink significantly decreased DPPH free radical scavenging activity and iron ion reducing power, except for 100% black tea proportion. Tannin compounds seems determining antioxidant activity.  Based on coefficient correlation between TPC or TFC and DPPH free radical scavenging activity or iron ion reducing power, the result showed that DPPH free radical scavenging activity was dominantly contributed by TPC and iron ion reducing power was determined by TPC and TFC. ABSTRAKPenelitian ini dilakukan untuk mengetahui potensi minuman daun beluntas teh hitam sebagai antioksidan pada berbagai proporsi. Penelitian ini menggunakan rancangan acak kelompok satu faktor, yaitu proporsi daun beluntas teh hitam meliputi 100:0; 75:25; 50:50; 25:75; dan 0:100% (b/b). Tiap faktor diulang sebanyak 5 kali. Parameter yang diamati pada penelitian ini meliputi kandungan metabolit sekunder, total fenolik (TPC), total flavonoid (TFC), kemampuan menangkal radikal bebas DPPH, dan kemampuan mereduksi ion besi. Data dianalisis secara statistik dengan Analysis of Variance (ANOVA) pada α = 5%, jika terdapat beda signifikan dilanjutkan dengan uji Duncan's Multiple Range Test (DMRT). Data dinyatakan dengan rata-rata ± standar deviasi.  Hasil menunjukkan bahwa metabolit sekunder yang terkandung dalam minuman daun beluntas teh hitam pada berbagai proporsi adalah alkaloid, flavonoid, fenolik, saponin, tannin, dan kardiak glikosida. Peningkatan proporsi teh hitam menambah intensitas senyawa alkaloid, fenolik, flavonoid, saponin, dan kardiak glikosida yang terdeteksi, tetapi kandungan senyawa tannin berkurang. Kandungan metabolit sekunder ini berkorelasi dengan total fenolik (TPC) dan total flavonoid (TFC). Peningkatan proporsi teh hitam dalam minuman menurunkan kemampuan menangkal radikal bebas DPPH dan kemampuan mereduksi ion besi, kecuali pada proporsi teh hitam 100%. Kandungan senyawa tannin dalam minuman menentukan aktivitas antioksidan. Berdasarkan koefisien korelasi antara TPC atau TFC dan kemampuan menangkal radikal bebas DPPH atau kemampuan mereduksi ion besi, hasil menunjukkan bahwa kemampuan menangkal radikal bebas DPPH dominan dikontribusi oleh TPC dan kemampuan mereduksi ion besi ditentukan oleh TPC dan TFC.

scholarly journals PHYTOCHEMICAL CHARACTERIZATION AND ANTIOXIDANT ACTIVITY OF THE NORTHERN MOROCCAN SPECIES: WITHANIA FRUTESCENS L.

2019 ◽  
pp. 276-279 ◽  
Author(s):  
ABDELFATTAH EL MOUSSAOUI ◽  
FATIMA ZAHRA JAWHARI ◽  
DALILA BOUSTA ◽  
AMINA BARI
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Antioxidant Capacity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Mineral Elements ◽  
Free Radical Scavenging ◽  
Butylated Hydroxytoluene ◽  
Phytochemical Analysis ◽  
Total Polyphenols

Objective: In this study, we were interested in qualitative, quantitative phytochemical characterization and evaluation of the antioxidant capacity of the total extracts of a plant from northern Morocco, the species selected for this study is Withania frutescens. Materials and Methods: Analysis of mineral elements by inductive coupling plasma-atomic absorption spectroscopy (ICP-AES), phytochemical screening, polyphenol and tannin assays, evaluation of antioxidant activity by the 1,2-diphenyl-β-picrylhydrazyl (DPPH) free radical scavenging method and reducing power. Results: The plant contains alkaloids, saponins, tannins, mucilages, and coumarins. It has a relatively high content of total polyphenols and tannins of 19.53±0.018 μg genetic generalized epilepsy/mg MS and 6.258±0.062 μg Eqcat/mg MS, respectively. The analysis of mineral elements by ICP-AES shows that our species is rich in mineral elements which are calcium, magnesium, and sodium, and it is devoid of metallic elements such as nickel, lead, cadmium, and cobalt. The evaluation of antioxidant activity by the DPPH free radical scavenging method shows that the half maximal inhibitory concentration of the tested extracts has an antiradical activity of about 0.056±0.008 μg/ml for the ethanol extract and 0.213±0.004 μg/ml for the methanol extract compared to the butylated hydroxytoluene value of 0.009±0.0004 μg/ml which was used as a reference. The reducing capacity test shows that methanolic extract has a high antioxidant capacity (0.213±0.006) compared to ethanolic extract (0.043±0.004) but remains low compared to ascorbic acid (0.003±0.0004) which was used as reference. Conclusion: Phytochemical analysis of W. frutescens shows that this plant is rich in high quantities of alkaloids, saponins, mucilage, tannins, and coumarins. It contains an average amount of total polyphenols and tannins that confer significant antioxidant activity to the plant studied.

Antioxidant, Antityrosinase Activity and Toxicity of Alpinia nigra Extracts

2019 ◽  
Vol 819 ◽  
pp. 111-117 ◽  
Author(s):  
Papatsiri Janyapanich ◽  
Chatchanok Kotipan ◽  
Kritsanat Teerachawalwong ◽  
Surawut Watana ◽  
Nopparat Nuntharatanapon
Keyword(s):  
Free Radical ◽  
Brine Shrimp ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Free Radical Scavenging ◽  
Aqueous Extracts ◽  
Mushroom Tyrosinase ◽  
Melanin Production ◽  
Brine Shrimp Lethality ◽  
Alpinia Nigra

Excessive of ultraviolet light causes abnormality of melanin production. Antioxidants and antityrosinase agents are able to reduce hyperpigmentation by interrupting the process of melanin production. The purpose of this study is to examine the antioxidant and antityrosinase activities as well as toxicity of both 80% ethanol and aqueous extracts of Alpinia nigra by DPPH free radical scavenging assay, mushroom tyrosinase assay and brine shrimp lethality bioassay. Alpinia nigra extracts showed positive result on antioxidant and antityrosinase activities. We found that extract of A. nigra’s leaf has the most effective activity of antioxidant and antityrosinase among other parts of this plant. The ethanol and aqueous extracts from the leaf of A. nigra at the concentration of 125 μg/mL showed % inhibition for free radical scavenging as 94.97% and 93.35%, respectively. The IC50 values of antioxidant were 39.83±16.21 and 46.33±15.22 μg/mL, respectively. In addition, ethanol extract of the leaf from A. nigra at the concentration of 1,000 μg/mL produced 92.61% inhibition of mushroom tyrosinase activity, whereas aqueous extract of A. nigra’s leaf at the same concentration produced 74.47% inhibition. The IC50 of antityrosinase activities were 142.81±13.32 and 406.88±66.43 μg/mL for ethanol and aqueous extracts, respectively. Moreover, the brine shrimp lethality bioassay showed that all extracts were non-toxic (LC50 >1,000 μg/mL). In conclusion, the ethanol extract of A. nigra’s leaf may be beneficial and provide the novel and safe source for antioxidant and whitening agent.

Evaluation of antioxidant activity of Melothria maderaspatana in vitro

2010 ◽  
Vol 5 (2) ◽  
pp. 224-230 ◽  
Author(s):  
Boobalan Raja ◽  
Kodukkur Pugalendi
Keyword(s):  
Hydrogen Peroxide ◽  
Antioxidant Activity ◽  
Free Radical ◽  
Hydroxyl Radical ◽  
Superoxide Anion ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Melothria Maderaspatana

AbstractIn this study, an aqueous extract of leaves from Melothria maderaspatana was tested for in vitro antioxidant activity. Free radical scavenging assays, such as hydroxyl radical, hydrogen peroxide, superoxide anion radical and 2,2-diphenyl-1-picryl hydrazyl (DPPH), 2,2’-azinobis-(3-ethyl-enzothiazoline-6-sulfonic acid) (ABTS) radical scavenging, and reducing power assay, were studied. The extract effectively scavenged hydroxyl radical, hydrogen peroxide and superoxide anion radicals. It also scavenged DPPH and ABTS radicals. Furthermore, it was found to have reducing power. All concentrations of leaf extract exhibited free radical scavenging and antioxidant power, and the preventive effects were in a dose-dependent manner. The antioxidant activities of the above were compared to standard antioxidants such as butylated hydroxytoluene (BHT), ascorbic acid, and α-tocopherol. The results obtained in the present study indicate that the M. maderaspatana extract could be considered a potential source of natural antioxidant.

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