scholarly journals Anti-Inflammatory Triterpenoids from the Caulophyllum robustum Maximin LPS-Stimulated RAW264.7 Cells

Molecules ◽  
2018 ◽  
Vol 23 (5) ◽  
pp. 1149 ◽  
Author(s):  
Bin-Hua Qin ◽  
Xin-Qiao Liu ◽  
Qiao-Yu Yuan ◽  
Jing Wang ◽  
Hai-Yan Han
Keyword(s):  
Raw264.7 Cells ◽  
Anti Inflammatory ◽  
Caulophyllum Robustum

Anti-inflammatory Effect of Allium hookeri Root Methanol Extract in LPS-induced RAW264.7 Cells

2012 ◽  
Vol 41 (11) ◽  
pp. 1645-1648 ◽  
Author(s):  
Chang-Hyun Kim ◽  
Mi-Ai Lee ◽  
Tae-Woon Kim ◽  
Ja Young Jang ◽  
Hyun Ju Kim
Keyword(s):  
Methanol Extract ◽  
Raw264.7 Cells ◽  
Allium Hookeri ◽  
Anti Inflammatory ◽  
Inflammatory Effect

scholarly journals Anti-Inflammatory Effect of Simonsinol on Lipopolysaccharide Stimulated RAW264.7 Cells through Inactivation of NF-κB Signaling Pathway

Molecules ◽  
2020 ◽  
Vol 25 (16) ◽  
pp. 3573
Author(s):  
Lian-Chun Li ◽  
Zheng-Hong Pan ◽  
De-Sheng Ning ◽  
Yu-Xia Fu
Keyword(s):  
Nitric Oxide ◽  
Signaling Pathway ◽  
Morphological Changes ◽  
Raw264.7 Cells ◽  
Enzyme Linked Immunosorbent Assay ◽  
Tnf Α ◽  
Anti Inflammatory ◽  
Factor Α ◽  
Oxide Synthase ◽  
Necrosis Factor

Simonsinol is a natural sesqui-neolignan firstly isolated from the bark of Illicium simonsii. In this study, the anti-inflammatory activity of simonsinol was investigated with a lipopolysaccharide (LPS)-stimulated murine macrophages RAW264.7 cells model. The results demonstrated that simonsinol could antagonize the effect of LPS on morphological changes of RAW264.7 cells, and decrease the production of nitric oxide (NO), tumor necrosis factor α (TNF-α), and interleukin 6 (IL-6) in LPS-stimulated RAW264.7 cells, as determined by Griess assay and enzyme-linked immunosorbent assay (ELISA). Furthermore, simonsinol could downregulate transcription of inducible nitric oxide synthase (iNOS), TNF-α, and IL-6 as measured by reverse transcription polymerase chain reaction (RT-PCR), and inhibit phosphorylation of the alpha inhibitor of NF-κB (IκBα) as assayed by Western blot. In conclusion, these data demonstrate that simonsinol could inhibit inflammation response in LPS-stimulated RAW264.7 cells through the inactivation of the nuclear transcription factor kappa-B (NF-κB) signaling pathway.

scholarly journals Anti-Neuroinflammatory and Anti-Inflammatory Activities of Phenylheptatriyne Isolated from the Flowers of Coreopsis lanceolata L. via NF-κB Inhibition and HO-1 Expression in BV2 and RAW264.7 Cells

2021 ◽  
Vol 22 (14) ◽  
pp. 7482
Author(s):  
Hwan Lee ◽  
Zhiming Liu ◽  
Chi-Su Yoon ◽  
Linsha Dong ◽  
Wonmin Ko ◽  
...  
Keyword(s):  
Silica Gel ◽  
Column Chromatography ◽  
Raw264.7 Cells ◽  
Necrosis Factor Alpha ◽  
Tnf Α ◽  
Etoac Fraction ◽  
Factor Alpha ◽  
Anti Inflammatory ◽  
And Oxidative Stress ◽  
Necrosis Factor

Aging is associated with immune disregulation and oxidative stress which lead to inflammation and neurodegenerative diseases. We have tried to identify the anti-neuroinflammatory and anti-inflammatory components of Coreopsis lanceolata L. The dried flowers of C. lanceolata were extracted with 70% EtOH, and the obtained extract was divided into CH2Cl2, EtOAc, n-BuOH, and H2O fractions. The CH2Cl2 fraction was separated using silica gel and C-18 column chromatography to yield phenylheptatriyne (1), 2′-hydroxy-3,4,4′-trimethoxychalcone (2), and 4′,7-dimethoxyflavanone (3). Additionally, the EtOAc fraction was subjected to silica gel, C-18, and Sephadex LH-20 column chromatography to yield 8-methoxybutin (4) and leptosidin (5). All the compounds isolated from C. lanceolata inhibited the production of nitric oxide (NO) in LPS-induced BV2 and RAW264.7 cells. In addition, phenylheptatriyne and 4′,7-dimethoxyflavanone reduced the secretion of inflammatory cytokines, tumor necrosis factor alpha (TNF-α), and interleukin (IL)-6. Among them, phenylheptatriyne was significantly downregulated in the expression of inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2). Subsequently, phenylheptatriyne also effectively inhibited nuclear factor-kappa B (NF-κB) activation in LPS-stimulated BV2 and RAW264.7 cells. Based on these results, the anti-neuroinflammatory effect of phenylheptatriyne isolated from C. lanceolata was confirmed, which may exert a therapeutic effect in treatment of neuroinflammation-related diseases.

scholarly journals Procyanidin A2, a polyphenolic compound, exerts anti-inflammatory and anti-oxidative activity in lipopolysaccharide-stimulated RAW264.7 cells

PLoS ONE ◽  
2020 ◽  
Vol 15 (8) ◽  
pp. e0237017
Author(s):  
Qin-Qin Wang ◽  
Hongwei Gao ◽  
Renyikun Yuan ◽  
Shan Han ◽  
Xin-Xing Li ◽  
...  
Keyword(s):  
Oxidative Activity ◽  
Raw264.7 Cells ◽  
Polyphenolic Compound ◽  
Anti Inflammatory

scholarly journals Anti-Inflammatory and Antiosteoclastogenic Activities of Parthenolide on Human Periodontal Ligament CellsIn Vitro

2014 ◽  
Vol 2014 ◽  
pp. 1-11 ◽  
Author(s):  
Xufang Zhang ◽  
Chen Fan ◽  
Yin Xiao ◽  
Xueli Mao
Keyword(s):  
Periodontal Ligament ◽  
Tooth Loss ◽  
Nuclear Factor Kappa B ◽  
Raw264.7 Cells ◽  
Erk Activation ◽  
Tanacetum Parthenium ◽  
Coculture Model ◽  
Anti Inflammatory ◽  
Potential Use

Periodontitis is an inflammatory disease that causes osteolysis and tooth loss. It is known that the nuclear factor kappa B (NF-κB) signalling pathway plays a key role in the progression of inflammation and osteoclastogenesis in periodontitis. Parthenolide (PTL), a sesquiterpene lactone extracted from the shoots ofTanacetum parthenium, has been shown to possess anti-inflammatory properties in various diseases. In the study reported herein, we investigated the effects of PTL on the inflammatory and osteoclastogenic response of human periodontal ligament-derived cells (hPDLCs) and revealed the signalling pathways in this process. Our results showed that PTL decreased NF-κB activation, I-κB degradation, and ERK activation in hPDLCs. PTL significantly reduced the expression of inflammatory (IL-1β, IL-6, and TNF-α) and osteoclastogenic (RANKL, OPG, and M-CSF) genes in LPS-stimulated hPDLCs. In addition, PTL attenuated hPDLC-induced osteoclastogenic differentiation of macrophages (RAW264.7 cells), as well as reducing gene expression of osteoclast-related markers in RAW264.7 cells in an hPDLC-macrophage coculture model. Taken together, these results demonstrate the anti-inflammatory and antiosteoclastogenic activities of PTL in hPDLCsin vitro. These data offer fundamental evidence supporting the potential use of PTL in periodontitis treatment.

scholarly journals The role of autophagy in the process of osseointegration around titanium implants with micro-nano topography promoted by osteoimmunity

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Ting Zhang ◽  
Mengyang Jiang ◽  
Xiaojie Yin ◽  
Peng Yao ◽  
Huiqiang Sun
Keyword(s):  
Titanium Implants ◽  
Eukaryotic Cells ◽  
Raw264.7 Cells ◽  
Control Group ◽  
Immune Microenvironment ◽  
Promote Cell Proliferation ◽  
Osteogenic Markers ◽  
Proliferation And Differentiation ◽  
Anti Inflammatory

AbstractOsteoimmunity plays an important role in the process of implant osseointegration. Autophagy is a conservative metabolic pathway of eukaryotic cells, but whether the interaction between autophagy and osteoimmunity plays a key role in osseointegration remains unclear. In this study, we prepared smooth titanium disks and micro-nano topography titanium disks, to study the immune microenvironment of RAW264.7 cells, and prepared the conditioned medium to study the effect of immune microenvironment on the osteogenesis and autophagy of MC3T3-E1 cells. Autophagy inhibitor 3-MA was used to inhibit autophagy to observe the change of expression of osteogenic markers. The results showed that the micro-nano topography titanium disks could stimulate RAW264.7 cells to differentiate into M2 type, forming an anti-inflammatory immune microenvironment; compared with the control group, the anti-inflammatory immune microenvironment promoted the proliferation and differentiation of osteoblasts better. The anti-inflammatory immune environment activated the autophagy level of osteoblasts, while the expression of osteogenic markers was down-regulated after inhibition of autophagy. These results indicate that anti-inflammatory immune microenvironment can promote cell proliferation and osteogenic differentiation, autophagy plays an important role in this process. This study further explains the mechanism of implant osseointegration in osteoimmune microenvironment, and provides reference for improving implant osseointegration.

scholarly journals Anti-Inflammatory Effect of Asterias amurensis Fatty Acids through NF-��B and MAPK Pathways against LPS-Stimulated RAW264.7 Cells

2018 ◽  
Vol 28 (10) ◽  
pp. 1635-1644 ◽  
Author(s):  
Chaiwat Monmai ◽  
Seok Hyeon Go ◽  
Il-shik Shin ◽  
SangGuan You ◽  
Dae-ok Kim ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Raw264.7 Cells ◽  
Asterias Amurensis ◽  
Mapk Pathways ◽  
Anti Inflammatory ◽  
Inflammatory Effect

scholarly journals Anti-Inflammatory Effect of the Extracts from Abeliophyllum distichum Nakai in LPS-Stimulated RAW264.7 Cells

2014 ◽  
Vol 27 (3) ◽  
pp. 209-214 ◽  
Author(s):  
Gwang Hun Park ◽  
Jae Ho Park ◽  
Hyun Ji Eo ◽  
Hun Min Song ◽  
Man Hyo Lee ◽  
...  
Keyword(s):  
Raw264.7 Cells ◽  
Anti Inflammatory ◽  
Abeliophyllum Distichum ◽  
Inflammatory Effect
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