scholarly journals Tissue-Specific Analysis of Secondary Metabolites Creates a Reliable Morphological Criterion for Quality Grading of Polygoni Multiflori Radix

Molecules ◽  
2018 ◽  
Vol 23 (5) ◽  
pp. 1115 ◽  
Author(s):  
Li Liang ◽  
Jun Xu ◽  
Zhi-Tao Liang ◽  
Xiao-Ping Dong ◽  
Hu-Biao Chen ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Morphological Criterion ◽  
Tissue Specific ◽  
Specific Analysis ◽  
Quality Grading

Mechanisms of glycolytic control during facultative anaerobiosis in a marine mollusc: tissue-specific analysis of glycogen phosphorylase and fructose-2,6-bisphosphate

1988 ◽  
Vol 66 (8) ◽  
pp. 1767-1771 ◽  
Author(s):  
Kenneth B. Storey
Keyword(s):  
Glycogen Phosphorylase ◽  
Retractor Muscle ◽  
Dramatic Reduction ◽  
Metabolic Rate Depression ◽  
Tissue Specific ◽  
Marine Mollusc ◽  
Energy Needs ◽  
Specific Analysis ◽  
Anaerobic Energy ◽  
Glycolytic Control

Changes in the activity of glycogen phosphorylase and the content of fructose-2,6-bisphosphate (F-2, 6-P2) were monitored in tissues of the whelk, Busycotypus canaliculatum, over a 21-h course of environmental anoxia. Tissue-specific responses to anoxia were seen with respect to phosphorylase content: in the radular retractor muscle and foot, the content of phosphorylase a expressed rose rapidly over the initial hours of anoxia (maximal increases were 4.3- and 2.5-fold, respectively) while in the gill, content dropped 2-fold during anoxia. Phosphorylase content was modulated by two mechanisms, changes in the percentage of enzyme in the active a form and changes in the total amount (a + b) of enzyme expressed. Anoxia stimulated a dramatic reduction in F-2,6-P2 content in five tissues. In the ventricle, content fell by 224-fold with a t1/2 of only 35 min. Levels in gill, radular retractor, hepatopancreas, and kidney fell to 2.5–3.5% of control values within the first 8 h of anoxia. F-2,6-P2 content in foot muscle was not altered during anoxia. Changes in glycogen phosphorylase activities and F-2,6-P2 contents help to produce tissue-specific responses of glycolysis to environmental anoxia that acknowledge competing metabolic demands including metabolic rate depression, changes in fuel use, anaerobic energy needs, and carbohydrate use for anabolic purposes.

Tissue specific analysis of bioconversion traits in the bioenergy grass Sorghum bicolor

2013 ◽  
Vol 50 ◽  
pp. 118-130 ◽  
Author(s):  
Joshua P. Vandenbrink ◽  
Ryan E. Hammonds ◽  
Roger N. Hilten ◽  
K.C. Das ◽  
J. Michael Henson ◽  
...  
Keyword(s):  
Sorghum Bicolor ◽  
Tissue Specific ◽  
Specific Analysis

scholarly journals Tissue‐Specific Analysis of Pharmacological Pathways

2018 ◽  
Vol 7 (7) ◽  
pp. 453-463 ◽  
Author(s):  
Yun Hao ◽  
Kayla Quinnies ◽  
Ronald Realubit ◽  
Charles Karan ◽  
Nicholas P. Tatonetti
Keyword(s):  
Tissue Specific ◽  
Specific Analysis

scholarly journals De novo transcriptome analysis of the critically endangered alpine Himalayan herb Nardostachys jatamansi reveals the biosynthesis pathway genes of tissue-specific secondary metabolites

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Nisha Dhiman ◽  
Anil Kumar ◽  
Dinesh Kumar ◽  
Amita Bhattacharya
Keyword(s):  
Secondary Metabolites ◽  
Transcriptome Analysis ◽  
Vegetative Growth ◽  
De Novo ◽  
Biosynthesis Pathway ◽  
De Novo Transcriptome ◽  
Tissue Specific ◽  
Nardostachys Jatamansi ◽  
Secondary Metabolite Biosynthesis

Abstract The study is the first report on de novo transcriptome analysis of Nardostachys jatamansi, a critically endangered medicinal plant of alpine Himalayas. Illumina GAIIx sequencing of plants collected during end of vegetative growth (August) yielded 48,411 unigenes. 74.45% of these were annotated using UNIPROT. GO enrichment analysis, KEGG pathways and PPI network indicated simultaneous utilization of leaf photosynthates for flowering, rhizome fortification, stress response and tissue-specific secondary metabolites biosynthesis. Among the secondary metabolite biosynthesis genes, terpenoids were predominant. UPLC-PDA analysis of in vitro plants revealed temperature-dependent, tissue-specific differential distribution of various phenolics. Thus, as compared to 25 °C, the phenolic contents of both leaves (gallic acid and rutin) and roots (p-coumaric acid and cinnamic acid) were higher at 15 °C. These phenolics accounted for the therapeutic properties reported in the plant. In qRT-PCR of in vitro plants, secondary metabolite biosynthesis pathway genes showed higher expression at 15 °C and 14 h/10 h photoperiod (conditions representing end of vegetative growth period). This provided cues for in vitro modulation of identified secondary metabolites. Such modulation of secondary metabolites in in vitro systems can eliminate the need for uprooting N. jatamansi from wild. Hence, the study is a step towards effective conservation of the plant.

scholarly journals A unique mouse strain expressing Cre recombinase for tissue-specific analysis of gene function in palate and kidney development

genesis ◽  
2007 ◽  
Vol 45 (10) ◽  
pp. 618-624 ◽  
Author(s):  
Yu Lan ◽  
Qingru Wang ◽  
Catherine E. Ovitt ◽  
Rulang Jiang
Keyword(s):  
Gene Function ◽  
Mouse Strain ◽  
Kidney Development ◽  
Cre Recombinase ◽  
Tissue Specific ◽  
Specific Analysis

Preparative laser capture microdissection and single-pot cell wall material preparation: a novel method for tissue-specific analysis

Planta ◽  
2006 ◽  
Vol 224 (1) ◽  
pp. 228-232 ◽  
Author(s):  
Guillermo Angeles ◽  
Jimmy Berrio-Sierra ◽  
Jean-Paul Joseleau ◽  
Philippe Lorimier ◽  
Andrée Lefèbvre ◽  
...  
Keyword(s):  
Cell Wall ◽  
Laser Capture Microdissection ◽  
Wall Material ◽  
Cell Wall Material ◽  
Tissue Specific ◽  
Specific Analysis ◽  
Novel Method ◽  
Laser Capture ◽  
Material Preparation
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