scholarly journals Comparative Targeted Proteomics of the Central Metabolism and Photosystems in SigE Mutant Strains of Synechocystis sp. PCC 6803

Molecules ◽  
2018 ◽  
Vol 23 (5) ◽  
pp. 1051 ◽  
Author(s):  
Yuma Tokumaru ◽  
Kiyoka Uebayashi ◽  
Masakazu Toyoshima ◽  
Takashi Osanai ◽  
Fumio Matsuda ◽  
...  
Keyword(s):  
Targeted Proteomics ◽  
Central Metabolism ◽  
Mutant Strains ◽  
Synechocystis Sp ◽  
Pcc 6803

scholarly journals Reduction of Photoautotrophic Productivity in the Cyanobacterium Synechocystis sp. Strain PCC 6803 by Phycobilisome Antenna Truncation

2012 ◽  
Vol 78 (17) ◽  
pp. 6349-6351 ◽  
Author(s):  
Lawrence E. Page ◽  
Michelle Liberton ◽  
Himadri B. Pakrasi
Keyword(s):  
Light Harvesting ◽  
Wild Type ◽  
Content Type ◽  
Photosynthetic Productivity ◽  
Mutant Strains ◽  
Light Harvesting Antenna ◽  
Culture Productivity ◽  
Synechocystis Sp ◽  
Pcc 6803

ABSTRACTTruncation of the algal light-harvesting antenna is expected to enhance photosynthetic productivity. The wild type and three mutant strains ofSynechocystissp. strain 6803 with a progressively smaller phycobilisome antenna were examined under different light and CO2conditions. Surprisingly, such antenna truncation resulted in decreased whole-culture productivity for this cyanobacterium.

scholarly journals Temperature enhanced succinate production concurrent with increased central metabolism turnover in the cyanobacterium Synechocystis sp. PCC 6803

2018 ◽  
Vol 48 ◽  
pp. 109-120 ◽  
Author(s):  
Tomohisa Hasunuma ◽  
Mami Matsuda ◽  
Yuichi Kato ◽  
Christopher John Vavricka ◽  
Akihiko Kondo
Keyword(s):  
Central Metabolism ◽  
Succinate Production ◽  
Synechocystis Sp ◽  
Pcc 6803

scholarly journals Minor pilin genes are involved in motility and natural competence in Synechocystis sp. PCC 6803

2020 ◽  
Author(s):  
Sabrina Oeser ◽  
Thomas Wallner ◽  
Nils Schuergers ◽  
Annegret Wilde ◽  
Lenka Bucinska ◽  
...  
Keyword(s):  
Cell Surface ◽  
Microarray Analysis ◽  
Natural Transformation ◽  
Type Iv Pili ◽  
Transcriptional Unit ◽  
Natural Competence ◽  
Type Iv ◽  
Mutant Strains ◽  
Synechocystis Sp ◽  
Pcc 6803

Cyanobacteria synthesize type IV pili, which are known to be essential for motility, adhesion and natural competence. They consist of long flexible fibres that are primarily composed of the major pilin PilA1 in Synechocystis sp. PCC 6803. In addition, Synechocystis encodes less abundant pilin-like proteins, which are known as minor pilins. The transcription of the minor pilin genes pilA5, pilA6 and pilA9-pilA11 is inversely regulated in response to different conditions. In this study, we show that the minor pilin PilA5 is essential for natural transformation but is dispensable for motility and flocculation. In contrast, a set of minor pilins encoded by the pilA9-slr2019 transcriptional unit are necessary for motility but are dispensable for natural transformation. Neither pilA5-pilA6 nor pilA9-slr2019 are essential for pilus assembly as mutant strains showed type IV pili on the cell surface. Microarray analysis demonstrated that the transcription levels of known and newly predicted minor pilin genes change in response to surface contact. A total of 120 genes were determined to have altered transcription between planktonic and surface growth. Among these genes, 13 are located on the pSYSM plasmid. The results of our study indicate that different minor pilins facilitate distinct pilus functions.

Sign in / Sign up

Export Citation Format

Share Document