scholarly journals Functions of the Duplicated hik31 Operons in Central Metabolism and Responses to Light, Dark, and Carbon Sources in Synechocystis sp. Strain PCC 6803

2011 ◽  
Vol 194 (2) ◽  
pp. 448-459 ◽  
Author(s):  
S. Nagarajan ◽  
D. M. Sherman ◽  
I. Shaw ◽  
L. A. Sherman
Keyword(s):  
Carbon Sources ◽  
Central Metabolism ◽  
Synechocystis Sp ◽  
Pcc 6803

scholarly journals Temperature enhanced succinate production concurrent with increased central metabolism turnover in the cyanobacterium Synechocystis sp. PCC 6803

2018 ◽  
Vol 48 ◽  
pp. 109-120 ◽  
Author(s):  
Tomohisa Hasunuma ◽  
Mami Matsuda ◽  
Yuichi Kato ◽  
Christopher John Vavricka ◽  
Akihiko Kondo
Keyword(s):  
Central Metabolism ◽  
Succinate Production ◽  
Synechocystis Sp ◽  
Pcc 6803

scholarly journals Comparative Targeted Proteomics of the Central Metabolism and Photosystems in SigE Mutant Strains of Synechocystis sp. PCC 6803

Molecules ◽  
2018 ◽  
Vol 23 (5) ◽  
pp. 1051 ◽  
Author(s):  
Yuma Tokumaru ◽  
Kiyoka Uebayashi ◽  
Masakazu Toyoshima ◽  
Takashi Osanai ◽  
Fumio Matsuda ◽  
...  
Keyword(s):  
Targeted Proteomics ◽  
Central Metabolism ◽  
Mutant Strains ◽  
Synechocystis Sp ◽  
Pcc 6803

scholarly journals Contribution of protein synthesis depression to poly-β-hydroxybutyrate accumulation in Synechocystis sp. PCC 6803 under nutrient-starved conditions

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Kazuho Hirai ◽  
Miki Nojo ◽  
Yosuke Sato ◽  
Mikio Tsuzuki ◽  
Norihiro Sato
Keyword(s):  
Protein Synthesis ◽  
Lipid Droplets ◽  
Carbon Sources ◽  
Cellular Proteins ◽  
Protein Synthesis Inhibitor ◽  
Wild Type ◽  
Synthesis Inhibitor ◽  
Synechocystis Sp ◽  
Pcc 6803

AbstractPoly-β-hydroxybutyrate (PHB) in cyanobacteria, which accumulates as energy and carbon sources through the action of photosynthesis, is expected to substitute for petroleum-based plastics. This study first demonstrated that PHB accumulation was induced, with the appearance of lipid droplets, in sulfur (S)-starved cells of a cyanobacterium, Synechocystis sp. PCC 6803, however, to a lower level than in nitrogen (N)- or phosphorus (P)-starved cells. Concomitantly found was repression of the accumulation of total cellular proteins in the S-starved cells to a similar level to that in N-starved cells, and a severer level than in P-starved cells. Intriguingly, PHB accumulation was induced in Synechocystis even under nutrient-replete conditions, upon repression of the accumulation of total cellular proteins through treatment of the wild type cells with a protein synthesis inhibitor, chloramphenicol, or through disruption of the argD gene for Arg synthesis. Meanwhile, the expression of the genes for PHB synthesis was hardly induced in S-starved cells, in contrast to their definite up-regulation in N- or P-starved cells. It therefore seemed that PHB accumulation in S-starved cells is achieved through severe repression of protein synthesis, but is smaller than in N- or P-starved cells, owing to little induction of the expression of PHB synthesis genes.

scholarly journals Expanding the toolbox for Synechocystis sp. PCC 6803: validation of replicative vectors and characterization of a novel set of promoters

2018 ◽  
Vol 3 (1) ◽  
Author(s):  
Eunice A Ferreira ◽  
Catarina C Pacheco ◽  
Filipe Pinto ◽  
José Pereira ◽  
Pedro Lamosa ◽  
...  
Keyword(s):  
Low Cost ◽  
Carbon Sources ◽  
Proof Of Concept ◽  
Gene Encoding ◽  
Metabolic Plasticity ◽  
Cell Factories ◽  
Wide Range ◽  
Synechocystis Sp ◽  
Pcc 6803

Abstract Cyanobacteria are promising ‘low-cost’ cell factories since they have minimal nutritional requirements, high metabolic plasticity and can use sunlight and CO2 as energy and carbon sources. The unicellular Synechocystis sp. PCC 6803, already considered the ‘green’ Escherichia coli, is the best studied cyanobacterium but to be used as an efficient and robust photoautotrophic chassis it requires a customized and well-characterized toolbox. In this context, we evaluated the possibility of using three self-replicative vectors from the Standard European Vector Architecture (SEVA) repository to transform Synechocystis. Our results demonstrated that the presence of the plasmid does not lead to an evident phenotype or hindered Synechocystis growth, being the vast majority of the cells able to retain the replicative plasmid even in the absence of selective pressure. In addition, a set of heterologous and redesigned promoters were characterized exhibiting a wide range of activities compared to the reference PrnpB, three of which could be efficiently repressed. As a proof-of-concept, from the expanded toolbox, one promoter was selected and assembled with the ggpS gene [encoding one of the proteins involved in the synthesis of the native compatible solute glucosylglycerol (GG)] and the synthetic device was introduced into Synechocystis using one of the SEVA plasmids. The presence of this device restored the production of the GG in a ggpS deficient mutant validating the functionality of the tools/device developed in this study.

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