scholarly journals Epigallocatechin Gallate-Modified Gelatin Sponges Treated by Vacuum Heating as a Novel Scaffold for Bone Tissue Engineering

Molecules ◽  
2018 ◽  
Vol 23 (4) ◽  
pp. 876 ◽  
Author(s):  
Yoshitomo Honda ◽  
Yoshihiro Takeda ◽  
Peiqi Li ◽  
Anqi Huang ◽  
Satoshi Sasayama ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Epigallocatechin Gallate ◽  
Vacuum Heating ◽  
Novel Scaffold

scholarly journals Synthesis and characterization of a novel scaffold for bone tissue engineering based on Wharton's jelly

2017 ◽  
Vol 105 (4) ◽  
pp. 1034-1045 ◽  
Author(s):  
Cristian Martínez ◽  
Carlos Fernández ◽  
Miguel Prado ◽  
Andres Ozols ◽  
Daniel G. Olmedo
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Wharton’S Jelly ◽  
Synthesis And Characterization ◽  
Wharton's Jelly ◽  
Novel Scaffold

scholarly journals Physical Properties and Biocompatibility of a Core-Sheath Structure Composite Scaffold for Bone Tissue Engineering In Vitro

2012 ◽  
Vol 2012 ◽  
pp. 1-9 ◽  
Author(s):  
Chuangjian Wang ◽  
Guolin Meng ◽  
Laquan Zhang ◽  
Zuo Xiong ◽  
Jian Liu
Keyword(s):  
Tissue Engineering ◽  
Physical Properties ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Composite Scaffold ◽  
Control Group ◽  
The Novel ◽  
Novel Scaffold ◽  
Sheath Structure

Scaffolds play a critical role in the practical realization of bone tissue engineering. The purpose of this study was to assess whether a core-sheath structure composite scaffold possesses admirable physical properties and biocompatibility in vitro. A novel scaffold composed of poly(lactic-co-glycolic acid)/β-tricalcium phosphate (PLGA/β-TCP) skeleton wrapped with Type I collagen via low-temperature deposition manufacturing (LDM) was prepared, and bone mesenchymal stem cells (BMSCs) were used to evaluate cell behavior on the scaffold. PLGA/β-TCP skeleton was chosen as the control group. Physical properties were evaluated by pority ratio, compressive strength, and Young’s modulus. Scanning electron microscope (SEM) was used to study morphology of cells. Hydrophilicity was evaluated by water absorption ratio. Cell proliferation was tested by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay (MTT). Osteogenic differentiation of BMSCs was evaluated by alkaline phosphates activity (ALP). The results indicated that physical properties of the novel scaffold were as good as those of the control group, hydrophilicity was observably better (P<0.01) than that of control group, and abilities of proliferation and osteogenic differentiation of BMSCs on novel scaffold were significantly greater (P<0.05) than those of control group, which suggests that the novel scaffold possesses preferable characteristics and have high value in bone tissue engineering.

scholarly journals Effect of Bone Morphogenic Protein-2-Loaded Mesoporous Strontium Substitution Calcium Silicate/Recycled Fish Gelatin 3D Cell-Laden Scaffold for Bone Tissue Engineering

Processes ◽  
2020 ◽  
Vol 8 (4) ◽  
pp. 493 ◽  
Author(s):  
Chun-Ta Yu ◽  
Fu-Ming Wang ◽  
Yen-Ting Liu ◽  
Hooi Yee Ng ◽  
Yi-Rong Jhong ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Calcium Silicate ◽  
Cellular Proliferation ◽  
Recent Decade ◽  
Engineering Research ◽  
Non Union ◽  
Enhanced Secretion ◽  
Novel Scaffold

Bone has a complex hierarchical structure with the capability of self-regeneration. In the case of critical-sized defects, the regeneration capabilities of normal bones are severely impaired, thus causing non-union healing of bones. Therefore, bone tissue engineering has since emerged to solve problems relating to critical-sized bone defects. Amongst the many biomaterials available on the market, calcium silicate-based (CS) cements have garnered huge interest due to their versatility and good bioactivity. In the recent decade, scientists have attempted to modify or functionalize CS cement in order to enhance the bioactivity of CS. Reports have been made that the addition of mesoporous nanoparticles onto scaffolds could enhance the bone regenerative capabilities of scaffolds. For this study, the main objective was to reuse gelatin from fish wastes and use it to combine with bone morphogenetic protein (BMP)-2 and Sr-doped CS scaffolds to create a novel BMP-2-loaded, hydrogel-based mesoporous SrCS scaffold (FGSrB) and to evaluate for its composition and mechanical strength. From this study, it was shown that such a novel scaffold could be fabricated without affecting the structural properties of FGSr. In addition, it was proven that FGSrB could be used for drug delivery to allow stable localized drug release. Such modifications were found to enhance cellular proliferation, thus leading to enhanced secretion of alkaline phosphatase and calcium. The above results showed that such a modification could be used as a potential alternative for future bone tissue engineering research.

scholarly journals 3D-Printing of Hierarchically Designed and Osteoconductive Bone Tissue Engineering Scaffolds

Materials ◽  
2020 ◽  
Vol 13 (8) ◽  
pp. 1836 ◽  
Author(s):  
Nicolas Söhling ◽  
Jonas Neijhoft ◽  
Vinzenz Nienhaus ◽  
Valentin Acker ◽  
Jana Harbig ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cell Survival ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Modular Design ◽  
Negative Impact ◽  
Frame Structure ◽  
Large Animal ◽  
Large Animal Models ◽  
Novel Scaffold

In Bone Tissue Engineering (BTE), autologous bone-regenerative cells are combined with a scaffold for large bone defect treatment (LBDT). Microporous, polylactic acid (PLA) scaffolds showed good healing results in small animals. However, transfer to large animal models is not easily achieved simply by upscaling the design. Increasing diffusion distances have a negative impact on cell survival and nutrition supply, leading to cell death and ultimately implant failure. Here, a novel scaffold architecture was designed to meet all requirements for an advanced bone substitute. Biofunctional, porous subunits in a load-bearing, compression-resistant frame structure characterize this approach. An open, macro- and microporous internal architecture (100 µm–2 mm pores) optimizes conditions for oxygen and nutrient supply to the implant’s inner areas by diffusion. A prototype was 3D-printed applying Fused Filament Fabrication using PLA. After incubation with Saos-2 (Sarcoma osteogenic) cells for 14 days, cell morphology, cell distribution, cell survival (fluorescence microscopy and LDH-based cytotoxicity assay), metabolic activity (MTT test), and osteogenic gene expression were determined. The adherent cells showed colonization properties, proliferation potential, and osteogenic differentiation. The innovative design, with its porous structure, is a promising matrix for cell settlement and proliferation. The modular design allows easy upscaling and offers a solution for LBDT.

scholarly journals Preparation, characterization, bioactivity and degradation behavior in vitro of copper-doped calcium polyphosphate as a candidate material for bone tissue engineering

RSC Advances ◽  
2017 ◽  
Vol 7 (67) ◽  
pp. 42614-42626 ◽  
Author(s):  
Chengrui Guo ◽  
Li Li ◽  
Shuangshuang Li ◽  
Yaping Wang ◽  
Xixun Yu
Keyword(s):  
Tissue Engineering ◽  
Trace Elements ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Human Body ◽  
Degradation Behavior ◽  
Calcium Polyphosphate ◽  
Essential Trace Elements ◽  
Novel Scaffold

In this study, copper, as one of the essential trace elements in the human body, was introduced into calcium polyphosphate (CPP) to prepare a novel scaffold in bone tissue engineering: copper-doped calcium polyphosphate (CCPP) scaffolds.

scholarly journals Enhanced Proliferation and Differentiation of Human Mesenchymal Stem Cell-laden Recycled Fish Gelatin/Strontium Substitution Calcium Silicate 3D Scaffolds

2020 ◽  
Vol 10 (6) ◽  
pp. 2168 ◽  
Author(s):  
Chun-Ta Yu ◽  
Fu-Ming Wang ◽  
Yen-Ting Liu ◽  
Alvin Kai-Xing Lee ◽  
Tsung-Li Lin ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Calcium Silicate ◽  
Clinical Applications ◽  
In Vitro Assays ◽  
Calcium Deposition ◽  
Fish Gelatin ◽  
3D Scaffolds ◽  
Novel Scaffold

Cell-encapsulated bioscaffold is a promising and novel method to allow fabrication of live functional organs for tissue engineering and regenerative medicine. However, traditional fabrication methods of 3D scaffolds and cell-laden hydrogels still face many difficulties and challenges. This study uses a newer 3D fabrication technique and the concept of recycling of an unutilized resource to fabricate a novel scaffold for bone tissue engineering. In this study, fish-extracted gelatin was incorporated with bioactive ceramic for bone tissue engineering, and with this we successfully fabricated a novel fish gelatin methacrylate (FG) polymer hydrogel mixed with strontium-doped calcium silicate powder (FGSr) 3D scaffold via photo-crosslinking. Our results indicated that the tensile strength of FGSr was almost 2.5-fold higher as compared to FG thus making it a better candidate for future clinical applications. The in-vitro assays illustrated that the FGSr scaffolds showed good biocompatibility with human Wharton jelly-derived mesenchymal stem cells (WJMSC), as well as enhancing the osteogenesis differentiation of WJMSC. The WJMSC-laden FGSr 3D scaffolds expressed a higher degree of alkaline phosphatase activity than those on cell-laden FG 3D scaffolds and this result was further proven with the subsequent calcium deposition results. Therefore, these results showed that 3D-printed cell-laden FGSr scaffolds had enhanced mechanical property and osteogenic-related behavior that made for a more suitable candidate for future clinical applications.

scholarly journals Graphene Oxide Enhances Chitosan-Based 3D Scaffold Properties for Bone Tissue Engineering

2019 ◽  
Vol 20 (20) ◽  
pp. 5077 ◽  
Author(s):  
Sorina Dinescu ◽  
Mariana Ionita ◽  
Simona-Rebeca Ignat ◽  
Marieta Costache ◽  
Anca Hermenean
Keyword(s):  
Tissue Engineering ◽  
Graphene Oxide ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Cell Attachment ◽  
Bone Development ◽  
Total Porosity ◽  
Stem Cell Differentiation ◽  
3D Scaffold ◽  
Novel Scaffold

The main goal of bone tissue engineering (BTE) is to refine and repair major bone defects based on bioactive biomaterials with distinct properties that can induce and support bone tissue formation. Graphene and its derivatives, such as graphene oxide (GO), display optimal properties for BTE, being able to support cell growth and proliferation, cell attachment, and cytoskeleton development as well as the activation of osteogenesis and bone development pathways. Conversely, the presence of GO within a polymer matrix produces favorable changes to scaffold morphologies that facilitate cell attachment and migration i.e., more ordered morphologies, greater surface area, and higher total porosity. Therefore, there is a need to explore the potential of GO for tissue engineering applications and regenerative medicine. Here, we aim to promote one novel scaffold based on a natural compound of chitosan, improved with 3 wt.% GO, for BTE approaches, considering its good biocompatibility, remarkable 3D characteristics, and ability to support stem cell differentiation processes towards the bone lineage.

Poly(lactic-co-glycolic acid)(PLGA)/TiO 2 nanotube bioactive composite as a novel scaffold for bone tissue engineering: In vitro and in vivo studies

Biologicals ◽  
2018 ◽  
Vol 53 ◽  
pp. 51-62 ◽  
Author(s):  
Hossein Eslami ◽  
Hamidreza Azimi Lisar ◽  
Tahereh Sadat Jafarzadeh Kashi ◽  
Mohammadreza Tahriri ◽  
Mojtaba Ansari ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Glycolic Acid ◽  
In Vivo Studies ◽  
Novel Scaffold

scholarly journals Interconnected porous hydroxyapatite ceramics for bone tissue engineering

2008 ◽  
Vol 6 (suppl_3) ◽  
Author(s):  
Hideki Yoshikawa ◽  
Noriyuki Tamai ◽  
Tsuyoshi Murase ◽  
Akira Myoui
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Animal Experiments ◽  
Average Diameter ◽  
Dimensional Structure ◽  
Uniform Size ◽  
Porous Hydroxyapatite ◽  
Hydroxyapatite Ceramics ◽  
Novel Scaffold

Several porous calcium hydroxyapatite (HA) ceramics have been used clinically as bone substitutes, but most of them possessed few interpore connections, resulting in pathological fracture probably due to poor bone formation within the substitute. We recently developed a fully interconnected porous HA ceramic (IP-CHA) by adopting the ‘foam-gel’ technique. The IP-CHA had a three-dimensional structure with spherical pores of uniform size (average 150 μm, porosity 75%), which were interconnected by window-like holes (average diameter 40 μm), and also demonstrated adequate compression strength (10–12 MPa). In animal experiments, the IP-CHA showed superior osteoconduction, with the majority of pores filled with newly formed bone. The interconnected porous structure facilitates bone tissue engineering by allowing the introduction of mesenchymal cells, osteotropic agents such as bone morphogenetic protein or vasculature into the pores. Clinically, we have applied the IP-CHA to treat various bony defects in orthopaedic surgery, and radiographic examinations demonstrated that grafted IP-CHA gained radiopacity more quickly than the synthetic HA in clinical use previously. We review the accumulated data on bone tissue engineering using the novel scaffold and on clinical application in the orthopaedic field.

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