scholarly journals Graphene Oxide Enhances Chitosan-Based 3D Scaffold Properties for Bone Tissue Engineering

2019 ◽  
Vol 20 (20) ◽  
pp. 5077 ◽  
Author(s):  
Sorina Dinescu ◽  
Mariana Ionita ◽  
Simona-Rebeca Ignat ◽  
Marieta Costache ◽  
Anca Hermenean
Keyword(s):  
Tissue Engineering ◽  
Graphene Oxide ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Cell Attachment ◽  
Bone Development ◽  
Total Porosity ◽  
Stem Cell Differentiation ◽  
3D Scaffold ◽  
Novel Scaffold

The main goal of bone tissue engineering (BTE) is to refine and repair major bone defects based on bioactive biomaterials with distinct properties that can induce and support bone tissue formation. Graphene and its derivatives, such as graphene oxide (GO), display optimal properties for BTE, being able to support cell growth and proliferation, cell attachment, and cytoskeleton development as well as the activation of osteogenesis and bone development pathways. Conversely, the presence of GO within a polymer matrix produces favorable changes to scaffold morphologies that facilitate cell attachment and migration i.e., more ordered morphologies, greater surface area, and higher total porosity. Therefore, there is a need to explore the potential of GO for tissue engineering applications and regenerative medicine. Here, we aim to promote one novel scaffold based on a natural compound of chitosan, improved with 3 wt.% GO, for BTE approaches, considering its good biocompatibility, remarkable 3D characteristics, and ability to support stem cell differentiation processes towards the bone lineage.

scholarly journals Synthetic Biodegradable Aliphatic Polyester Nanocomposites Reinforced with Nanohydroxyapatite and/or Graphene Oxide for Bone Tissue Engineering Applications

Nanomaterials ◽  
2019 ◽  
Vol 9 (4) ◽  
pp. 590 ◽  
Author(s):  
Yuchao Li ◽  
Chengzhu Liao ◽  
Sie Chin Tjong
Keyword(s):  
Tissue Engineering ◽  
Graphene Oxide ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Cell Attachment ◽  
Porous Scaffolds ◽  
Aliphatic Polyester ◽  
Engineering Applications ◽  
Aliphatic Polyesters ◽  
Nanocomposite Scaffolds

This paper provides review updates on the current development of bionanocomposites with polymeric matrices consisting of synthetic biodegradable aliphatic polyesters reinforced with nanohydroxyaptite (nHA) and/or graphene oxide (GO) nanofillers for bone tissue engineering applications. Biodegradable aliphatic polyesters include poly(lactic acid) (PLA), polycaprolactone (PCL) and copolymers of PLA-PGA (PLGA). Those bionanocomposites have been explored for making 3D porous scaffolds for the repair of bone defects since nHA and GO enhance their bioactivity and biocompatibility by promoting biomineralization, bone cell adhesion, proliferation and differentiation, thus facilitating new bone tissue formation upon implantation. The incorporation of nHA or GO into aliphatic polyester scaffolds also improves their mechanical strength greatly, especially hybrid GO/nHA nanofilllers. Those mechanically strong nanocomposite scaffolds can support and promote cell attachment for tissue growth. Porous scaffolds fabricated from conventional porogen leaching, and thermally induced phase separation have many drawbacks inducing the use of organic solvents, poor control of pore shape and pore interconnectivity, while electrospinning mats exhibit small pores that limit cell infiltration and tissue ingrowth. Recent advancement of 3D additive manufacturing allows the production of aliphatic polyester nanocomposite scaffolds with precisely controlled pore geometries and large pores for the cell attachment, growth, and differentiation in vitro, and the new bone formation in vivo.

scholarly journals In vitro cytocompatibility evaluation of chitosan/graphene oxide 3D scaffold composites designed for bone tissue engineering

2014 ◽  
Vol 24 (6) ◽  
pp. 2249-2256 ◽  
Author(s):  
Sorina Dinescu ◽  
Mariana Ionita ◽  
Andreea Madalina Pandele ◽  
Bianca Galateanu ◽  
Horia Iovu ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Graphene Oxide ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
3D Scaffold

Silicate-doped nano-hydroxyapatite/graphene oxide composite reinforced fibrous scaffolds for bone tissue engineering

2018 ◽  
Vol 32 (10) ◽  
pp. 1392-1405 ◽  
Author(s):  
Ali Deniz Dalgic ◽  
Ammar Z. Alshemary ◽  
Ayşen Tezcaner ◽  
Dilek Keskin ◽  
Zafer Evis
Keyword(s):  
Tissue Engineering ◽  
Graphene Oxide ◽  
Bone Tissue Engineering ◽  
Protein Adsorption ◽  
Bone Tissue ◽  
Three Dimensional ◽  
Osteosarcoma Cell ◽  
In Vitro Biocompatibility ◽  
Microscopy Techniques

In this study, novel graphene oxide–incorporated silicate-doped nano-hydroxyapatite composites were prepared and their potential use for bone tissue engineering was investigated by developing an electrospun poly(ε-caprolactone) scaffold. Nanocomposite groups were synthesized to have two different ratios of graphene oxide (2 and 4 wt%) to evaluate the effect of graphene oxide incorporation and groups with different silicate-doped nano-hydroxyapatite content was prepared to investigate optimum concentrations of both silicate-doped nano-hydroxyapatite and graphene oxide. Three-dimensional poly(ε-caprolactone) scaffolds were prepared by wet electrospinning and reinforced with silicate-doped nano-hydroxyapatite/graphene oxide nanocomposite groups to improve bone regeneration potency. Microstructural and chemical characteristics of the scaffolds were investigated by X-ray diffraction, Fourier transform infrared spectroscope and scanning electron microscopy techniques. Protein adsorption and desorption on material surfaces were studied using fetal bovine serum. Presence of graphene oxide in the scaffold, dramatically increased the protein adsorption with decreased desorption. In vitro biocompatibility studies were conducted using human osteosarcoma cell line (Saos-2). Electrospun scaffold group that was prepared with effective concentrations of silicate-doped nano-hydroxyapatite and graphene oxide particles (poly(ε-caprolactone) – 10% silicate-doped nano-hydroxyapatite – 4% graphene oxide) showed improved adhesion, spreading, proliferation and alkaline phosphatase activity compared to other scaffold groups.

scholarly journals Enhanced Cell Proliferation and Osteogenesis Differentiation through a Combined Treatment of Poly-L-Lysine-Coated PLGA/Graphene Oxide Hybrid Fiber Matrices and Electrical Stimulation

2020 ◽  
Vol 2020 ◽  
pp. 1-15
Author(s):  
Jiaqi Zhu ◽  
Zhiping Qi ◽  
Changjun Zheng ◽  
Pan Xue ◽  
Chuan Fu ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cell Proliferation ◽  
Graphene Oxide ◽  
Electrical Stimulation ◽  
Bone Tissue Engineering ◽  
Bone Regeneration ◽  
Bone Tissue ◽  
Cellular Response ◽  
Hybrid Fiber ◽  
Fiber Matrices

Bone tissue engineering scaffold provides an effective treatment for bone defect repair. Biodegradable bone scaffold made of various synthetic and natural materials can be used as bone substitutes and grafts for defect site, which has great potential to support bone regeneration. Regulation of cell-scaffold material interactions is an important factor for modulating the cellular activity in bone tissue engineering scaffold applications. Thus, the hydrophilic, mechanical, and chemical properties of scaffold materials directly affect the results of bone regeneration and functional recovery. In this study, a poly-L-lysine (PLL) surface-modified poly(lactic-co-glycolic acid) (PLGA)/graphene oxide (GO) (PLL-PLGA/GO) hybrid fiber matrix was fabricated for bone tissue regeneration. Characterization of the resultant hybrid fiber matrices was done using scanning electron microscopy (SEM), contact angle, and a material testing machine. According to the results obtained from the test above, the PLL-PLGA/GO hybrid fiber matrices exhibited high wettability and mechanical strength. The special surface characteristics of PLL-PLGA/GO hybrid fiber matrices were more beneficial for protein adsorption and inhibit the proliferation of pathogens. Moreover, the enhanced regulation of MC3T3-E1 cell proliferation and differentiation was observed, when the resultant hybrid fiber matrices were combined with electrical stimulation (ES). The cellular response of MC3T3-E1 cells including cell adhesion, proliferation, alkaline phosphatase (ALP) activity, calcium deposition, and osteogenesis-related gene expression was significantly enhanced with the synergistic effect of resultant hybrid fiber matrices and ES. These data indicate that the PLL-PLGA/GO hybrid fiber matrices supported the cellular response in terms of cell proliferation and osteogenesis differentiation in the presence of electrical stimulation, which could be a potential treatment for bone defect.

scholarly journals Preparation and biological properties of silk fibroin/nano-hydroxyapatite/graphene oxide scaffolds with an oriented channel-like structure

RSC Advances ◽  
2020 ◽  
Vol 10 (17) ◽  
pp. 10118-10128 ◽  
Author(s):  
Lu Wang ◽  
Min Fang ◽  
Yijing Xia ◽  
Jiaxin Hou ◽  
Xiaoru Nan ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Graphene Oxide ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Silk Fibroin ◽  
Biological Properties ◽  
Hybrid Scaffold

A novel SF/nHAp/GO hybrid scaffold with oriented channel-like structure in bone tissue engineering.

PCL and PCL/PLA Scaffolds for Bone Tissue Regeneration

2013 ◽  
Vol 683 ◽  
pp. 168-171 ◽  
Author(s):  
Tatiana Patrício ◽  
Antonio Gloria ◽  
Paulo J. Bártolo
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Tissue Regeneration ◽  
Cell Attachment ◽  
Bone Tissue Regeneration ◽  
Engineering Applications ◽  
Printing System

This paper investigates the use of PCL and PCL/PLA scaffolds, produced using a novel additive biomanufacturing system called BioCell Printing, for bone tissue engineering applications. Results show that the BioCell Printing system produces scaffolds with regular and reproducible architecture, presenting no toxicity and enhancing cell attachment and proliferation. It was also possible to observe that the addition of PLA to PCL scaffolds strongly improves the biomechanical performance of the constructs.

Development of a nanocomposite scaffold of gelatin–alginate–graphene oxide for bone tissue engineering

2019 ◽  
Vol 133 ◽  
pp. 592-602 ◽  
Author(s):  
Shiv Dutt Purohit ◽  
Rakesh Bhaskar ◽  
Hemant Singh ◽  
Indu Yadav ◽  
Mukesh Kumar Gupta ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Graphene Oxide ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Nanocomposite Scaffold

Herbally Painted Biofunctional Scaffolds with Improved Osteoinductivity for Bone Tissue Engineering

2019 ◽  
Vol 41 ◽  
pp. 49-68 ◽  
Author(s):  
Shivaji Kashte ◽  
Gajanan Arbade ◽  
R.K. Sharma ◽  
Sachin Kadam
Keyword(s):  
Tissue Engineering ◽  
Cell Proliferation ◽  
Tensile Strength ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Cell Attachment ◽  
Biological Properties ◽  
Osteogenic Potential ◽  
Composite Scaffolds ◽  
Alizarin Red

In the bone tissue engineering composite scaffolds with osteogenic potential are emerging as the new tool. Here, we investigated the graphene (GP), graphene oxide (GO) andCissusquadrangularis(CQ) callus extract for their spontaneous osteoinductive potential. Electrospun poly ε-caprolactone (PCL) sheets were painted with varying combination GP, GO and CQ solutions as ink. The prepared PCL-GO, PCL-GO-CQ, PCL-GP and PCL-GP-CQ scaffolds were characterized for their physical, mechanical and biological properties. Addition of GO, GP, GO-CQ and GP-CQ to PCL enhanced roughness, wettability, Yield strength and tensile strength, biocompatibility .significantly. Presence of GO and CQ in PCL-GO-CQ scaffolds, while GP and CQ in PCL-GP-CQ scaffolds showed synergistic effect on the biocompatibility, Cell attachment,cell proliferation of human umbilical Wharton’s jelly derived mesenchymal stem cells (hUCMSCs) and their differentiation into osteoblasts by 21stday in culture without osteogenic differentiation media or any growth factors. Same is confirmed by the Alizarin red S staining and Von kossa staining. The combination of PCL-GO-CQ scaffold prepared by novel paint method was found to be the most potential in bone tissue engineering.

Development of new graphene oxide incorporated tricomponent scaffolds with polysaccharides and hydroxyapatite and study of their osteoconductivity on MG-63 cell line for bone tissue engineering

RSC Advances ◽  
2015 ◽  
Vol 5 (51) ◽  
pp. 41135-41143 ◽  
Author(s):  
R. Rajesh ◽  
Y. Dominic Ravichandran
Keyword(s):  
Tissue Engineering ◽  
Graphene Oxide ◽  
Bone Tissue Engineering ◽  
Cell Line ◽  
Bone Tissue ◽  
Gellan Gum ◽  
First Time

GO–alginate–HAP, GO–amylopectin–HAP and GO–gellan gum–HAP were prepared and characterized and their osteoconductivity were checked for the first time.

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