scholarly journals Development of Phaleria macrocarpa (Scheff.) Boerl Fruits Using Response Surface Methodology Focused on Phenolics, Flavonoids and Antioxidant Properties

Molecules ◽  
2018 ◽  
Vol 23 (4) ◽  
pp. 724 ◽  
Author(s):  
Khurul Mohamed Mahzir ◽  
Siti Abd Gani ◽  
Uswatun Hasanah Zaidan ◽  
Mohd Halmi
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Antioxidant Properties ◽  
Phaleria Macrocarpa

scholarly journals Development of Phaleria macrocarpa (Scheff.) Boerl Fruits Using Response Surface Methodology On Phenolic, Flavonoid and Antioxidants Properties

2018 ◽  
Author(s):  
Khurul Ain Mohamed Mahzir ◽  
Siti Salwa Abdul Gani ◽  
Nor Fadzillah Mokhtar
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Extraction Time ◽  
Feed Ratio ◽  
Total Phenolic ◽  
Extraction Temperature ◽  
Phaleria Macrocarpa

In this study, the optimal condition for the extraction of antioxidants from the fruit Buah Mahkota Dewa (Phaleria macrocarpa) was determined by using Response Surface Methodology (RSM). The optimization was applied using central composite design (CCD) to investigate the three independent variables, namely extraction temperature (oC), extraction time (minutes) and extraction solvent to-feed ratio (%v/v) on the responses of free radical scavenging activity (DPPH), ferric ion reducing power assay (FRAP), total phenolic content (TPC) and total flavonoid content (TFC).The optimal conditions for the antioxidants extraction were found to be extraction temperature (64oC), extraction time (66 minutes) and solvent to-feed ratio (75 %v/v) with the highest percentage yield of DPPH, FRAP, TPC and TFC were 86.85%, 7.47%, 292.86 mg/g and 3.22 mg/g respectively. Moreover, the data were subjected to response surface methodology (RSM) and the results showed that the polynomial equations for all models were significant, did not show lack of fit, and presented adjusted determination coefficients (R2) above 99%, proving the yield of phenolic, flavonoid and antioxidants activities obtained experimentally were close to the predicted values and the suitability of the model employed in RSM to optimize the extraction conditions. Hence, in this study, the fruit from P.macrocarpa could be considered to have the strong antioxidant ability and can be used in various cosmeceutical or medicinal applications.

scholarly journals Optimization of Growth and Carotenoid Production by Haloferax mediterranei Using Response Surface Methodology

Marine Drugs ◽  
2018 ◽  
Vol 16 (10) ◽  
pp. 372 ◽  
Author(s):  
Zaida Montero-Lobato ◽  
Adrián Ramos-Merchante ◽  
Juan Fuentes ◽  
Ana Sayago ◽  
Ángeles Fernández-Recamales ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Large Scale ◽  
Antioxidant Properties ◽  
Carotenoid Content ◽  
Scale Production ◽  
Optimal Conditions ◽  
Haloferax Mediterranei ◽  
Carotenoid Production ◽  
Total Carotenoid Content

Haloferax mediterranei produces C50 carotenoids that have strong antioxidant properties. The response surface methodology (RSM) tool helps to accurately analyze the most suitable conditions to maximize C50 carotenoids production by haloarchaea. The effects of temperature (15–50 °C), pH (4−10), and salinity (5–28% NaCl (w/v)) on the growth and carotenoid content of H. mediterranei were analyzed using the RSM approach. Growth was determined by measuring the turbidity at 600 nm. To determine the carotenoid content, harvested cells were lysed by freeze/thawing, then re-suspended in acetone and the total carotenoid content determined by measuring the absorbance at 494 nm. The analysis of carotenoids was performed by an HPLC system coupled with mass spectrometry. The results indicated the theoretical optimal conditions of 36.51 or 36.81 °C, pH of 8.20 or 8.96, and 15.01% or 12.03% (w/v) salinity for the growth of haloarchaea (OD600 = 12.5 ± 0.64) and production of total carotenoids (3.34 ± 0.29 mg/L), respectively. These conditions were validated experimentally for growth (OD600 = 13.72 ± 0.98) and carotenoid production (3.74 ± 0.20 mg/L). The carotenoid profile showed four isomers of bacterioruberin (89.13%). Our findings suggest that the RSM approach is highly useful for determining optimal conditions for large-scale production of bacterioruberin by haloarchaea.

scholarly journals OPTIMIZATION OF EXTRACTION PARAMETERS FOR FUCOXANTHIN, GALLIC ACID AND RUTIN FROM NITZSCHIA THERMALIS

2019 ◽  
Vol 13 (1) ◽  
Author(s):  
Vasfiye Hazal Özyur ◽  
Ayşegül Erdoğan ◽  
Zeliha Zeliha Demirel ◽  
Meltem Conk Dalay ◽  
Semih Ötleş
Keyword(s):  
Response Surface Methodology ◽  
Gallic Acid ◽  
Response Surface ◽  
Desirability Function ◽  
Antioxidant Properties ◽  
Value Added ◽  
Extraction Time ◽  
Solvent Ratio ◽  
Extraction Temperature ◽  
Extraction Parameters

Recently, microalgae have become important in their health, and cosmetic applications since they are viewed as new sources of carotenoids. Fucoxanthin is also a type of carotenoid. The anti-diabetic, anti-obesity, anti-cancer, and antioxidant properties of fucoxanthin have been widely reported. Since these valuable properties, they also represent a valuable resource of nutraceuticals for functional food applications. This study aims to determine the amount of fucoxanthin, gallic acid, and rutin in Nitzschia thermalis obtained from the Ege University Microalgae Culture Collection. The extraction parameters have been optimized using response surface methodology. The extraction temperature (25, 35, and 45°C), the extraction time (10, 20, and 30 min) and the biomass/solvent ratio (0.005, 0.001, and 0.015 g ml-1) have been assessed as response variables in the Box – Behnken design. The amount of fucoxanthin was determined by the C30 column at 450 nm, while both the amount of gallic acid and rutin were separated in the C18 column at 275 nm by HPLC-DAD. In the present study, the optimum extraction conditions providing the maximum amount of fucoxantin, gallic acid, and rutin were selected by applying the “desirability” function approach in response surface methodology. Finally, the temperature has been determined to be 27.30°C, the extraction time 10 minutes, and the biomass ratio 0.05 g ml-1. Under these conditions, the optimum fucoxanthin level has been determined as 5.8702 mg g-1, the gallic acid level as 0.0140 mg g-1, and the rutin level as 0.0496 mg g-1. The findings are in good agreement with international published values for fucoxanthin content. In addition, response surface methodology was shown to be an effective technique for optimising extraction conditions for maximum fucoxanthin yield. In conclusion, these findings may be applied in the development of extraction methodologies for value added microalgea products as well as can serve as a reference for the extraction of fucoxanthin having high gallic acid and rutin from other brown microalgae, and therefore it could potentially be applied in both pharmaceutical and food industries.

scholarly journals Optimization of Extraction of Total Phenolic and Total Flavonoid from Seeds of Alpinia blepharocalyx K. Schum. Using The Response Surface Methodology

2019 ◽  
Vol 57 (3B) ◽  
pp. 105
Author(s):  
Thanh Tan Nguyen ◽  
Huyen Thi Thanh Mai ◽  
Tuan Ngoc Nguyen ◽  
Quang Dinh Tran ◽  
Thang Dinh Tran
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Antioxidant Properties ◽  
Total Flavonoid ◽  
Extraction Time ◽  
Total Phenolic ◽  
Extraction Temperature ◽  
Flavonoid Compounds ◽  
Phenolic And Flavonoid ◽  
Phenolic And Flavonoid Compounds

Seeds of Alpinia blepharocalyx contain high amounts of phenolic and flavonoid compounds with potential antioxidant properties. In this study, the effects of the extraction method on the recovery of phenolic and flavonoid compounds from Alpinia blepharocalyx seeds were investigated. Response surface methodology (RSM) has been used to optimize the extraction conditions of total phenolic and total flavonoid from seeds of Alpinia blepharocalyx. A Box-Behnken design was used to investigate the effects of four independent variables, namely extraction temperature (°C), extraction time (min), (v/m) and ethanol concentration (%) on the responses: total phenolic content (TPC) and yield. The optimal conditions obtained from response RSM were 52.66 % v/v for the solvent composition, 62.34 (°C) for extraction temperature, 34.48/1 (ml/g) for solvent/material ratio and 125.42 (min) for extraction time. The experimental values of TPC, TFC and yield were 39.31±0.05 mgGAE/g, 12.75±0.07 mgCE/g and 6.97±0.05%, respectively.   

Optimization of oil yield of Phaleria macrocarpa seed using response surface methodology and its fatty acids constituents

2014 ◽  
Vol 52 ◽  
pp. 405-412 ◽  
Author(s):  
J. Azmir ◽  
I.S.M. Zaidul ◽  
M.M. Rahman ◽  
K.M. Sharif ◽  
F. Sahena ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Response Surface Methodology ◽  
Response Surface ◽  
Oil Yield ◽  
Phaleria Macrocarpa

scholarly journals Enzymatic inactivation and antioxidant properties of blackberry juice after thermoultrasound: Optimization using response surface methodology

2017 ◽  
Vol 34 ◽  
pp. 371-379 ◽  
Author(s):  
Alicia Cervantes-Elizarrarás ◽  
Javier Piloni-Martini ◽  
Esther Ramírez-Moreno ◽  
Ernesto Alanís-García ◽  
Norma Güemes-Vera ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Antioxidant Properties ◽  
Enzymatic Inactivation

scholarly journals Optimization of the Drum Drying Parameters and Citric Acid Level to Produce Purple Sweet Potato (Ipomoea batatas L.) Powder Using Response Surface Methodology

Foods ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1378
Author(s):  
Sri Sampath Janaka Senevirathna ◽  
Nurul Shazini Ramli ◽  
Ezzat Mohamad Azman ◽  
Nurul Hanisah Juhari ◽  
Roselina Karim
Keyword(s):  
Response Surface Methodology ◽  
Citric Acid ◽  
Response Surface ◽  
Sweet Potato ◽  
Ipomoea Batatas ◽  
Antioxidant Properties ◽  
Processing Condition ◽  
Anthocyanin Content ◽  
Total Anthocyanin ◽  
Purple Sweet Potato

Purple sweet potato (PSP) is a rich source of anthocyanins, but the anthocyanin content and color can be affected by the drying method and processing condition. Response surface methodology (RSM) with a Box–Behnken design (BBD) was used to investigate the effects of citric acid (CA) concentration, steam pressure (SP) and rotation speed (DS) on the physicochemical and functional properties of drum-dried purple sweet potato powder (PSPP). The anthocyanins of the PSPP were analyzed using mass spectrometry with electrospray ionization and twelve anthocyanins were identified. The results indicated that the moisture content (4.80 ± 0.17–9.97 ± 0.03%) and water activity (0.290 ± 0.004–0.47 ± 0.001) (p < 0.05) decreased with increasing drum temperature as well as with reduced drum rotating speed. CA had a significant (p < 0.05) effect on the color and total anthocyanin content (101.83 ± 2.20–124.09 ± 2.89 mg/100 g) of the PSPP. High SP and low DS negatively affected the antioxidant properties of the PSPP. DPPH value of the PSPP ranged from 20.41 ± 0.79 to 30.79 ± 1.00 μmol TE/g. The optimal parameters were achieved at 0.59% CA, 499.8 kPa SP and 3 rpm DS.

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