scholarly journals Biodegradable Poly(Amino Ester) with Aromatic Backbone as Efficient Nonviral Gene Delivery Vectors

Molecules ◽  
2017 ◽  
Vol 22 (4) ◽  
pp. 566 ◽  
Author(s):  
Qiang Liu ◽  
Rong-Chuan Su ◽  
Wen-Jing Yi ◽  
Zhi-Gang Zhao
Keyword(s):  
Gene Delivery ◽  
Nonviral Gene Delivery ◽  
Amino Ester ◽  
Gene Delivery Vectors ◽  
Biodegradable Poly

scholarly journals The transition from linear to highly branched poly(β-amino ester)s: Branching matters for gene delivery

2016 ◽  
Vol 2 (6) ◽  
pp. e1600102 ◽  
Author(s):  
Dezhong Zhou ◽  
Lara Cutlar ◽  
Yongsheng Gao ◽  
Wei Wang ◽  
Jonathan O’Keeffe-Ahern ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Transfection Efficiency ◽  
Gene Transfection ◽  
Branched Polymers ◽  
Nonviral Gene Delivery ◽  
Great Promise ◽  
Amino Ester ◽  
Design And Synthesis ◽  
Gene Delivery Vectors

Nonviral gene therapy holds great promise but has not delivered treatments for clinical application to date. Lack of safe and efficient gene delivery vectors is the major hurdle. Among nonviral gene delivery vectors, poly(β-amino ester)s are one of the most versatile candidates because of their wide monomer availability, high polymer flexibility, and superior gene transfection performance both in vitro and in vivo. However, to date, all research has been focused on vectors with a linear structure. A well-accepted view is that dendritic or branched polymers have greater potential as gene delivery vectors because of their three-dimensional structure and multiple terminal groups. Nevertheless, to date, the synthesis of dendritic or branched polymers has been proven to be a well-known challenge. We report the design and synthesis of highly branched poly(β-amino ester)s (HPAEs) via a one-pot “A2 + B3 + C2”–type Michael addition approach and evaluate their potential as gene delivery vectors. We find that the branched structure can significantly enhance the transfection efficiency of poly(β-amino ester)s: Up to an 8521-fold enhancement in transfection efficiency was observed across 12 cell types ranging from cell lines, primary cells, to stem cells, over their corresponding linear poly(β-amino ester)s (LPAEs) and the commercial transfection reagents polyethyleneimine, SuperFect, and Lipofectamine 2000. Moreover, we further demonstrate that HPAEs can correct genetic defects in vivo using a recessive dystrophic epidermolysis bullosa graft mouse model. Our findings prove that the A2 + B3 + C2 approach is highly generalizable and flexible for the design and synthesis of HPAEs, which cannot be achieved by the conventional polymerization approach; HPAEs are more efficient vectors in gene transfection than the corresponding LPAEs. This provides valuable insight into the development and applications of nonviral gene delivery and demonstrates great prospect for their translation to a clinical environment.

Lipid-Based Nanoparticles as Nonviral Gene Delivery Vectors

2013 ◽  
pp. 269-279 ◽  
Author(s):  
Daniele Pezzoli ◽  
Anna Kajaste-Rudnitski ◽  
Roberto Chiesa ◽  
Gabriele Candiani
Keyword(s):  
Gene Delivery ◽  
Nonviral Gene Delivery ◽  
Gene Delivery Vectors

Nonviral Gene Delivery Vectors Use Syndecan-Dependent Transport Mechanisms in Filopodia To Reach the Cell Surface

ACS Nano ◽  
2012 ◽  
Vol 6 (8) ◽  
pp. 7521-7532 ◽  
Author(s):  
Zia ur Rehman ◽  
Klaas A. Sjollema ◽  
Jeroen Kuipers ◽  
Dick Hoekstra ◽  
Inge S. Zuhorn
Keyword(s):  
Gene Delivery ◽  
Cell Surface ◽  
Nonviral Gene Delivery ◽  
Transport Mechanisms ◽  
Gene Delivery Vectors ◽  
Dependent Transport

Engineered Glycated Amino Dendritic Polymers as Specific Nonviral Gene Delivery Vectors Targeting the Receptor for Advanced Glycation End Products

2014 ◽  
Vol 25 (6) ◽  
pp. 1151-1161 ◽  
Author(s):  
M. Dolores Giron-Gonzalez ◽  
Arturo Morales-Portillo ◽  
Alfonso Salinas-Castillo ◽  
F. Javier Lopez-Jaramillo ◽  
Fernando Hernandez-Mateo ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Advanced Glycation End Products ◽  
Nonviral Gene Delivery ◽  
Dendritic Polymers ◽  
Advanced Glycation ◽  
Gene Delivery Vectors ◽  
Glycation End Products ◽  
End Products

scholarly journals Multi-armed poly(L-glutamic acid)-graft-oligoethylenimine copolymers as efficient nonviral gene delivery vectors

2010 ◽  
Vol 12 (1) ◽  
pp. 64-76 ◽  
Author(s):  
Lei Chen ◽  
Huayu Tian ◽  
Jie Chen ◽  
Xuesi Chen ◽  
Yubin Huang ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Glutamic Acid ◽  
Nonviral Gene Delivery ◽  
Gene Delivery Vectors

scholarly journals Cationic Amino Acid Based Lipids as Effective Nonviral Gene Delivery Vectors for Primary Cultured Neurons

2013 ◽  
Vol 4 (12) ◽  
pp. 1514-1519 ◽  
Author(s):  
Yumiko Aoshima ◽  
Ryosuke Hokama ◽  
Keitaro Sou ◽  
Satya Ranjan Sarker ◽  
Kabuto Iida ◽  
...  
Keyword(s):  
Amino Acid ◽  
Gene Delivery ◽  
Nonviral Gene Delivery ◽  
Cultured Neurons ◽  
Cationic Amino Acid ◽  
Gene Delivery Vectors ◽  
Primary Cultured Neurons

Alkyl sulfonyl derivatized PAMAM-G2dendrimers as nonviral gene delivery vectors with improved transfection efficiencies

2011 ◽  
Vol 9 (3) ◽  
pp. 851-864 ◽  
Author(s):  
Julia Morales-Sanfrutos ◽  
Alicia Megia-Fernandez ◽  
Fernando Hernandez-Mateo ◽  
Ma Dolores Giron-Gonzalez ◽  
Rafael Salto-Gonzalez ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Nonviral Gene Delivery ◽  
Gene Delivery Vectors

Gemini Amphiphiles: A Novel Class of Nonviral Gene Delivery Vectors

2010 ◽  
Vol 27 (3) ◽  
pp. 237-278 ◽  
Author(s):  
Mukesh Kumar ◽  
Kaustubh Jinturkar ◽  
M.R. Yadav ◽  
Ambikanandan Misra
Keyword(s):  
Gene Delivery ◽  
Nonviral Gene Delivery ◽  
Gene Delivery Vectors ◽  
Gemini Amphiphiles
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