scholarly journals Isolation and Characterization of Phenolic Compounds and Anthocyanins from Murta (Ugni molinae Turcz.) Fruits. Assessment of Antioxidant and Antibacterial Activity

Molecules ◽  
2015 ◽  
Vol 20 (4) ◽  
pp. 5698-5713 ◽  
Author(s):  
Maria Junqueira-Gonçalves ◽  
Lina Yáñez ◽  
Carolina Morales ◽  
Muriel Navarro ◽  
Rodrigo A. Contreras ◽  
...  
Biomolecules ◽  
2019 ◽  
Vol 9 (12) ◽  
pp. 847 ◽  
Author(s):  
Sónia A. O. Santos ◽  
Rafael Félix ◽  
Adriana C. S. Pais ◽  
Sílvia M. Rocha ◽  
Armando J. D. Silvestre

The current interest of the scientific community for the exploitation of high-value compounds from macroalgae is related to the increasing knowledge of their biological activities and health benefits. Macroalgae phenolic compounds, particularly phlorotannins, have gained particular attention due to their specific bioactivities, including antioxidant, antiproliferative, or antidiabetic. Notwithstanding, the characterization of macroalgae phenolic compounds is a multi-step task, with high challenges associated with their isolation and characterization, due to the highly complex and polysaccharide-rich matrix of macroalgae. Therefore, this fraction is far from being fully explored. In fact, a critical revision of the extraction and characterization methodologies already used in the analysis of phenolic compounds from macroalgae is lacking in the literature, and it is of uttermost importance to compile validated methodologies and discourage misleading practices. The aim of this review is to discuss the state-of-the-art of phenolic compounds already identified in green, red, and brown macroalgae, reviewing their structural classification, as well as critically discussing extraction methodologies, chromatographic separation techniques, and the analytical strategies for their characterization, including information about structural identification techniques and key spectroscopic profiles. For the first time, mass spectrometry data of phlorotannins, a chemical family quite exclusive of macroalgae, is compiled and discussed.


2015 ◽  
Vol 17 ◽  
pp. 979
Author(s):  
Alka Choudhary ◽  
Raj Kumar ◽  
Ravi Bihari Srivastava ◽  
Sunil Kumar Surapaneni ◽  
Kulbhushan Tikoo ◽  
...  

2018 ◽  
Vol 1 (2) ◽  
Author(s):  
A. Kwaji ◽  
H. M. Adamu ◽  
I. Y. Chindo ◽  
R. Atiko

The present study is aimed at the isolation and characterization of betulin from Entada africana.  A dichloromethane soluble portion of the stem bark methanol/acetone (1:1 v/v) extract was subjected to gradient elution using ethyl acetate in hexane (5 – 30 %) on an open column. A pure compound was obtained with Rf = 0.61 in hexane/ethyl acetate (8:2 v/v) after repeated washing and recrystallization from methanol and coded Enac1. The pure compound was analyzed using IR, 1H & 13C NMR and GC-MS. Clinical isolates of Escherichia coli, Klebsiella pneumoniae, Salmonella typhi and Staphylococcus aureus were used to assess the antibacterial activity of the pure compound while its preliminary Cytotoxicity was evaluated using brine shrimp nauplii. Based on the spectroscopic data obtained and in comparison with literature, the isolated compound was identified as betulin. The minimum inhibitory concentration (MIC) of betulin ranged between 62.50 - 250.00 µg/mL for all the four bacterial isolates in the study while its fifty percent lethal concentration (LC50) was 10.00 µg/mL. Significant Cytotoxicity with moderate antibacterial activity was observed. The study therefore justifies the existence of bioactive compounds in the stem bark of Entada africana and its use in traditional medicine.


1985 ◽  
Vol 33 (2) ◽  
pp. 527-531 ◽  
Author(s):  
SHOJI YAHARA ◽  
MAYUMI SATOSHIRO ◽  
ITSUO NISHIOKA ◽  
TETSURO NAGASAWA ◽  
HIKOKICHI OURA

2015 ◽  
Vol 16 ◽  
pp. 183-193 ◽  
Author(s):  
Alka Choudhary ◽  
Raj Kumar ◽  
Ravi Bihari Srivastava ◽  
Sunil Kumar Surapaneni ◽  
Kulbhushan Tikoo ◽  
...  

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