scholarly journals Preparative Separation of Alkaloids from Picrasma quassioides (D. Don) Benn. by Conventional and pH-Zone-Refining Countercurrent Chromatography

Molecules ◽  
2014 ◽  
Vol 19 (7) ◽  
pp. 8752-8761 ◽  
Author(s):  
Qinghai Zhang ◽  
Xikai Shu ◽  
Feng Jing ◽  
Xiao Wang ◽  
Changhu Lin ◽  
...  
Keyword(s):  
Countercurrent Chromatography ◽  
Zone Refining ◽  
Preparative Separation ◽  
Picrasma Quassioides

scholarly journals Preparative separation of structural isomeric pentacyclic triterpene oleanolic acid and ursolic acid from natural products by pH-zone-refining countercurrent chromatography

RSC Advances ◽  
2019 ◽  
Vol 9 (66) ◽  
pp. 38860-38866
Author(s):  
Chaoyue Wang ◽  
Xiang Wang ◽  
Shanshan Zhao ◽  
Wenyu Sun ◽  
Shengqiang Tong
Keyword(s):  
Natural Products ◽  
Ursolic Acid ◽  
Oleanolic Acid ◽  
Eriobotrya Japonica ◽  
Countercurrent Chromatography ◽  
Zone Refining ◽  
Preparative Separation ◽  
Pentacyclic Triterpene ◽  
Apple Peels

pH-zone-refining countercurrent chromatography was investigated in preparative separation of oleanolic acid and ursolic acid from three different natural products, Aralia chinensis, apple peels and Eriobotrya japonica Thunb.

scholarly journals Preparative separation of three isoquinoline alkaloids from Berberidis radix by pH-zone-refining countercurrent chromatography

2018 ◽  
Vol 24 (1) ◽  
pp. 1-7 ◽  
Author(s):  
Changlei Sun ◽  
Wei Zhao ◽  
Wenjuan Duan ◽  
Peng Yang ◽  
Bingtian Yang ◽  
...  
Keyword(s):  
Herbal Medicine ◽  
Volume Ratio ◽  
Ionization Mass ◽  
Countercurrent Chromatography ◽  
Zone Refining ◽  
Preparative Separation ◽  
Bioactive Constituents ◽  
Two Phase ◽  
Traditional Chinese Herbal Medicine ◽  
Chemical Structures

Berberidis radix, as a traditional Chinese herbal medicine, is well known as the main source of berberine. This herbal medicine has been used for the treatment of many diseases and alkaloids including jatrorrhizine, palmatine, and berberine are major bioactive constituents. Few literatures report the preparative separation of these alkaloids from Berberidis radix. In the present study, the combinative application of ultrasonic extraction and pH-zone-refining countercurrent chromatography was used to extract and purify these alkaloids. The PZRCCC separation was performed in a two-phase solvent system composed of chloroform? methanol?water (4:3:3 volume ratio), where 40 mM HCl was added in the upper aqueous stationary phase as a retainer and 10 mM triethylamine was added in the lower organic mobile phase as an eluter. From a crude sample of 1.5 g, palmatine (251.6 mg), berberine (392.5 mg), and jatrorrhizine (412.6 mg) were obtained in a single run with purities of all being over 93.0%, analyzed by HPLC. The chemical structures of the separated alkaloids were identified by electrospray ionization?mass spectrometry (ESI-MS), 1H-NMR and 13C-NMR.

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