scholarly journals Surface-Functionalized Hyperbranched Poly(Amido Acid) Magnetic Nanocarriers for Covalent Immobilization of a Bacterial γ-Glutamyltranspeptidase

Molecules ◽  
2014 ◽  
Vol 19 (4) ◽  
pp. 4997-5012 ◽  
Author(s):  
Tzong-Yuan Juang ◽  
Shao-Ju Kan ◽  
Yi-Yu Chen ◽  
Yi-Lin Tsai ◽  
Min-Guan Lin ◽  
...  
Keyword(s):  
Covalent Immobilization ◽  
Amido Acid ◽  
Hyperbranched Poly ◽  
Magnetic Nanocarriers

Changing the Wound: Covalent Immobilization of the Epidermal Growth Factor

2021 ◽  
Author(s):  
VijayKrishna Raghunathan ◽  
Shin Ae Park ◽  
Nihar M. Shah ◽  
Christopher M. Reilly ◽  
Leandro Teixeira ◽  
...  
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Covalent Immobilization ◽  
Epidermal Growth

scholarly journals Optical Monitoring of the Production Quality of Si-Nanoribbon Chips Intended for the Detection of ASD-Associated Oligonucleotides

Micromachines ◽  
2021 ◽  
Vol 12 (2) ◽  
pp. 147
Author(s):  
Kristina A. Malsagova ◽  
Tatyana O. Pleshakova ◽  
Vladimir P. Popov ◽  
Igor N. Kupriyanov ◽  
Rafael A. Galiullin ◽  
...  
Keyword(s):  
Production Quality ◽  
Autism Spectrum ◽  
Covalent Immobilization ◽  
Biological Macromolecules ◽  
Label Free ◽  
Raman Scattering Spectroscopy ◽  
Dna Oligonucleotides ◽  
Highly Sensitive ◽  
Highly Sensitive Detection

Gas-phase etching and optical lithography were employed for the fabrication of a silicon nanoribbon chip (Si-NR chip). The quality of the so-fabricated silicon nanoribbons (Si-NRs) was monitored by optical Raman scattering spectroscopy. It was demonstrated that the structures of the Si-NRs were virtually defect-free, meaning they could be used for highly sensitive detection of biological macromolecules. The Si-NR chips were then used for the highly sensitive nanoelectronics detection of DNA oligonucleotides (oDNAs), which represent synthetic analogs of 106a-5p microRNA (miR-106a-5p), associated with the development of autism spectrum disorders in children. The specificity of the analysis was attained by the sensitization of the Si-NR chip sur-face by covalent immobilization of oDNA probes, whose nucleotide sequence was complementary to the known sequence of miR-106a-5p. The use of the Si-NR chip was demonstrated to al-low for the rapid label-free real-time detection of oDNA at ultra-low (~10−17 M) concentrations.

A simple method to construct a low-cost immunosensor based on a dithiol-functionalized polydopamine platform

2021 ◽  
Author(s):  
Bo Liu ◽  
Luanying Yang ◽  
Gang Wang ◽  
Sha He ◽  
Xiaobo Wang ◽  
...  
Keyword(s):  
Detection Limit ◽  
Linear Response ◽  
Low Cost ◽  
Covalent Immobilization ◽  
The Self ◽  
Simple Method ◽  
Low Detection Limit

A simple and low-cost electrochemical CEA immunosensor was investigated via the self-polymerization of dopamine and a dithiol compound spacer for the covalent immobilization of antibodies. The designed CEA immunosensor exhibited a linear response and a low detection limit.

scholarly journals Distribution and Transport of CO2 in Hydrated Hyperbranched Poly(ethylenimine) Membranes: A Molecular Dynamics Simulation Approach

ACS Omega ◽  
2021 ◽  
Vol 6 (4) ◽  
pp. 3390-3398
Author(s):  
Kyung Il Kim ◽  
Robin Lawler ◽  
Hyun June Moon ◽  
Pavithra Narayanan ◽  
Miles A. Sakwa-Novak ◽  
...  
Keyword(s):  
Molecular Dynamics ◽  
Molecular Dynamics Simulation ◽  
Dynamics Simulation ◽  
Simulation Approach ◽  
Hyperbranched Poly

scholarly journals Green Production of Cladribine by Using Immobilized 2′-Deoxyribosyltransferase from Lactobacillus delbrueckii Stabilized through a Double Covalent/Entrapment Technology

Biomolecules ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 657
Author(s):  
Cintia Wanda Rivero ◽  
Natalia Soledad García ◽  
Jesús Fernández-Lucas ◽  
Lorena Betancor ◽  
Gustavo Pablo Romanelli ◽  
...  
Keyword(s):  
Silica Nanoparticles ◽  
Enzymatic Synthesis ◽  
Calcium Alginate ◽  
Covalent Immobilization ◽  
Chemical Processes ◽  
Lactobacillus Delbrueckii ◽  
Proof Of Concept ◽  
Enzymatic Production ◽  
Green Production ◽  
Efficient Alternative

Nowadays, enzyme-mediated processes offer an eco-friendly and efficient alternative to the traditional multistep and environmentally harmful chemical processes. Herein we report the enzymatic synthesis of cladribine by a novel 2′-deoxyribosyltransferase (NDT)-based combined biocatalyst. To this end, Lactobacillus delbrueckii NDT (LdNDT) was successfully immobilized through a two-step immobilization methodology, including a covalent immobilization onto glutaraldehyde-activated biomimetic silica nanoparticles followed by biocatalyst entrapment in calcium alginate. The resulting immobilized derivative, SiGPEI 25000-LdNDT-Alg, displayed 98% retained activity and was shown to be active and stable in a broad range of pH (5–9) and temperature (30–60 °C), but also displayed an extremely high reusability (up to 2100 reuses without negligible loss of activity) in the enzymatic production of cladribine. Finally, as a proof of concept, SiGPEI 25000-LdNDT-Alg was successfully employed in the green production of cladribine at mg scale.

Hyperbranched Poly(ethylene glycol)s:  A New Class of Ion-Conducting Materials

1996 ◽  
Vol 29 (11) ◽  
pp. 3831-3838 ◽  
Author(s):  
Craig J. Hawker ◽  
Fengkui Chu ◽  
Peter J. Pomery ◽  
David J. T. Hill
Keyword(s):  
Ethylene Glycol ◽  
Poly Ethylene Glycol ◽  
New Class ◽  
Hyperbranched Poly ◽  
Conducting Materials ◽  
Ion Conducting ◽  
Poly Ethylene
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