Covalent immobilization of 4-pyridinepropanol monolayers on Si(111) surfaces: Axially ligation F16CoPc on pyridine moieties

2019 ◽  
Vol 480 ◽  
pp. 235-242 ◽  
Author(s):  
Ali Alavi ◽  
Pooya Tahay ◽  
Nasser Safari
Keyword(s):  
Covalent Immobilization

Changing the Wound: Covalent Immobilization of the Epidermal Growth Factor

2021 ◽  
Author(s):  
VijayKrishna Raghunathan ◽  
Shin Ae Park ◽  
Nihar M. Shah ◽  
Christopher M. Reilly ◽  
Leandro Teixeira ◽  
...  
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Covalent Immobilization ◽  
Epidermal Growth

scholarly journals Optical Monitoring of the Production Quality of Si-Nanoribbon Chips Intended for the Detection of ASD-Associated Oligonucleotides

Micromachines ◽  
2021 ◽  
Vol 12 (2) ◽  
pp. 147
Author(s):  
Kristina A. Malsagova ◽  
Tatyana O. Pleshakova ◽  
Vladimir P. Popov ◽  
Igor N. Kupriyanov ◽  
Rafael A. Galiullin ◽  
...  
Keyword(s):  
Production Quality ◽  
Autism Spectrum ◽  
Covalent Immobilization ◽  
Biological Macromolecules ◽  
Label Free ◽  
Raman Scattering Spectroscopy ◽  
Dna Oligonucleotides ◽  
Highly Sensitive ◽  
Highly Sensitive Detection

Gas-phase etching and optical lithography were employed for the fabrication of a silicon nanoribbon chip (Si-NR chip). The quality of the so-fabricated silicon nanoribbons (Si-NRs) was monitored by optical Raman scattering spectroscopy. It was demonstrated that the structures of the Si-NRs were virtually defect-free, meaning they could be used for highly sensitive detection of biological macromolecules. The Si-NR chips were then used for the highly sensitive nanoelectronics detection of DNA oligonucleotides (oDNAs), which represent synthetic analogs of 106a-5p microRNA (miR-106a-5p), associated with the development of autism spectrum disorders in children. The specificity of the analysis was attained by the sensitization of the Si-NR chip sur-face by covalent immobilization of oDNA probes, whose nucleotide sequence was complementary to the known sequence of miR-106a-5p. The use of the Si-NR chip was demonstrated to al-low for the rapid label-free real-time detection of oDNA at ultra-low (~10−17 M) concentrations.

A simple method to construct a low-cost immunosensor based on a dithiol-functionalized polydopamine platform

2021 ◽  
Author(s):  
Bo Liu ◽  
Luanying Yang ◽  
Gang Wang ◽  
Sha He ◽  
Xiaobo Wang ◽  
...  
Keyword(s):  
Detection Limit ◽  
Linear Response ◽  
Low Cost ◽  
Covalent Immobilization ◽  
The Self ◽  
Simple Method ◽  
Low Detection Limit

A simple and low-cost electrochemical CEA immunosensor was investigated via the self-polymerization of dopamine and a dithiol compound spacer for the covalent immobilization of antibodies. The designed CEA immunosensor exhibited a linear response and a low detection limit.

scholarly journals Green Production of Cladribine by Using Immobilized 2′-Deoxyribosyltransferase from Lactobacillus delbrueckii Stabilized through a Double Covalent/Entrapment Technology

Biomolecules ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 657
Author(s):  
Cintia Wanda Rivero ◽  
Natalia Soledad García ◽  
Jesús Fernández-Lucas ◽  
Lorena Betancor ◽  
Gustavo Pablo Romanelli ◽  
...  
Keyword(s):  
Silica Nanoparticles ◽  
Enzymatic Synthesis ◽  
Calcium Alginate ◽  
Covalent Immobilization ◽  
Chemical Processes ◽  
Lactobacillus Delbrueckii ◽  
Proof Of Concept ◽  
Enzymatic Production ◽  
Green Production ◽  
Efficient Alternative

Nowadays, enzyme-mediated processes offer an eco-friendly and efficient alternative to the traditional multistep and environmentally harmful chemical processes. Herein we report the enzymatic synthesis of cladribine by a novel 2′-deoxyribosyltransferase (NDT)-based combined biocatalyst. To this end, Lactobacillus delbrueckii NDT (LdNDT) was successfully immobilized through a two-step immobilization methodology, including a covalent immobilization onto glutaraldehyde-activated biomimetic silica nanoparticles followed by biocatalyst entrapment in calcium alginate. The resulting immobilized derivative, SiGPEI 25000-LdNDT-Alg, displayed 98% retained activity and was shown to be active and stable in a broad range of pH (5–9) and temperature (30–60 °C), but also displayed an extremely high reusability (up to 2100 reuses without negligible loss of activity) in the enzymatic production of cladribine. Finally, as a proof of concept, SiGPEI 25000-LdNDT-Alg was successfully employed in the green production of cladribine at mg scale.

Cytocompatible polyurethanes from fatty acids through covalent immobilization of collagen

2013 ◽  
Vol 73 (5) ◽  
pp. 690-697 ◽  
Author(s):  
Rodolfo J. González-Paz ◽  
Ana M. Ferreira ◽  
Clara Mattu ◽  
Francesca Boccafoschi ◽  
Gerard Lligadas ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Covalent Immobilization

Novel Method for the Covalent Immobilization of Oligonucleotides via Diels—Alder Bioconjugation

ChemInform ◽  
2004 ◽  
Vol 35 (8) ◽  
Author(s):  
Hallie A. Latham-Timmons ◽  
Andreas Wolter ◽  
J. Shawn Roach ◽  
Rubina Giare ◽  
Michael Leuck
Keyword(s):  
Covalent Immobilization ◽  
Diels Alder ◽  
Novel Method
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