scholarly journals Identification of Absorbed Constituents in the Rabbit Plasma and Cerebrospinal Fluid after Intranasal Administration of Asari Radix et Rhizoma by HS-SPME-GC-MS and HPLC-APCI-IT-TOF-MSn

Molecules ◽  
2014 ◽  
Vol 19 (4) ◽  
pp. 4857-4879 ◽  
Author(s):  
Chen Li ◽  
Feng Xu ◽  
De-Mei Xie ◽  
Yu Jing ◽  
Ming-Ying Shang ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Intranasal Administration ◽  
Rabbit Plasma

scholarly journals Intranasal administration of the chemotherapeutic perillyl alcohol results in selective delivery to the cerebrospinal fluid in rats

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Geetika Nehra ◽  
Shannon Andrews ◽  
Joan Rettig ◽  
Michael N. Gould ◽  
Jill D. Haag ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Brain Tumors ◽  
Nasal Mucosa ◽  
Brain Cancer ◽  
Intranasal Administration ◽  
High Performance ◽  
Early Stage ◽  
Plasma Concentrations ◽  
Perillyl Alcohol ◽  
Systemic Absorption

AbstractPerillyl alcohol (POH) has been extensively studied for the treatment of peripheral and primary brain tumors. The intranasal route of administration has been preferred for dosing POH in early-stage clinical trials associated with promising outcomes in primary brain cancer. However, it is unclear how intranasal POH targets brain tumors in these patients. Multiple studies indicate that intranasally applied large molecules may enter the brain and cerebrospinal fluid (CSF) through direct olfactory and trigeminal nerve-associated pathways originating in the nasal mucosa that bypass the blood–brain barrier. It is unknown whether POH, a small molecule subject to extensive nasal metabolism and systemic absorption, may also undergo direct transport to brain or CSF from the nasal mucosa. Here, we compared CSF and plasma concentrations of POH and its metabolite, perillic acid (PA), following intranasal or intravascular POH application. Samples were collected over 70 min and assayed by high-performance liquid chromatography. Intranasal administration resulted in tenfold higher CSF-to-plasma ratios for POH and tenfold higher CSF levels for PA compared to equal dose intravascular administration. Our preclinical results demonstrate POH undergoes direct transport from the nasal mucosa to the CSF, a finding with potential significance for its efficacy as an intranasal chemotherapeutic for brain cancer.

Pharmacokinetics of tramadol in rat plasma and cerebrospinal fluid after intranasal administration

2008 ◽  
Vol 60 (9) ◽  
pp. 1149-1154 ◽  
Author(s):  
Yan Zhao ◽  
Tao Tao ◽  
Jinjin Wu ◽  
Jiaxin Pi ◽  
Ning He ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Intranasal Administration ◽  
Rat Plasma

Blood–cerebrospinal fluid barrier to arginine-vasopressin, desmopressin and desglycinamide arginine-vasopressin in the dog

1982 ◽  
Vol 93 (3) ◽  
pp. 319-325 ◽  
Author(s):  
V. T. Y. Ang ◽  
J. S. Jenkins
Keyword(s):  
Cerebrospinal Fluid ◽  
Intravenous Injection ◽  
Arginine Vasopressin ◽  
Half Life ◽  
Intranasal Administration ◽  
Route Of Administration ◽  
Total Plasma ◽  
Intranasal Route ◽  
Plasma Half Life

The passage of 125I-labelled arginine-vasopressin (AVP) and its analogues desmopressin (DDAVP) and desglycinamide arginine-vasopressin (DGAVP) into cerebrospinal fluid (CSF) has been studied in the dog. After intravenous injection or infusion of these peptides radioactive sustances were found in the CSF in amounts ranging from 0·5 to 1·4% of the total plasma radioactivity. However, only DDAVP could be identified in the CSF as the unmetabolized peptide. This observation may be related to the long plasma half-life of DDAVP which was found to be 50 min, compared with a half-life of 13 min for AVP and 8 min for DGAVP. After the intranasal administration of either [3H]AVP or 125I-labelled AVP similar results were obtained. Radioactivity was again present in the CSF but no AVP could be identified. These observations showed that the intranasal route of administration provides no increased access to the CSF. The existence of a blood–CSF barrier to AVP is confirmed and indicates that the concentrations of the hormone normally found in CSF arise from sources other than the blood.

Plasma Prostaglandins of the E and F Series in Rabbits during Fever Induced by Newcastle Disease Virus, E. coli-Endotoxin, or Endogenous Pyrogen

1974 ◽  
Vol 29 (11-12) ◽  
pp. 773-776 ◽  
Author(s):  
W Philipp-Dormston ◽  
R Siegert
Keyword(s):  
Cerebrospinal Fluid ◽  
Body Temperature ◽  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Carotid Arteries ◽  
Prostaglandin E ◽  
Rabbit Plasma ◽  
Endogenous Pyrogen ◽  
E Coli

Abstract Prostaglandins of the E and F series in rabbit plasma were estimated by radioimmunoassay after chromatographic separation during fever following the intravenous injection of Newcastle disease virus, E. coli-endotoxin, or endogenous pyrogen. Although concentrations of prostaglandins from both series are increased considerably by the three pyrogens, there seemed to be no correlation between prostaglandin concentrations in plasma and body temperature. These findings were supported by the fact, that after the injection of tritiated prostaglandin E1 into the carotid arteries of normal or feverish animals no radioactivity was detectable in cerebrospinal fluid. The enhanced prostaglandin concentrations in plasma night be responsible for various pathological effects observed during fever such as diarrhoea and abortion.

scholarly journals Elevated cerebrospinal fluid and blood concentrations of oxytocin following its intranasal administration in humans

2013 ◽  
Vol 3 (1) ◽  
Author(s):  
Nadine Striepens ◽  
Keith M. Kendrick ◽  
Vanessa Hanking ◽  
Rainer Landgraf ◽  
Ullrich Wüllner ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Intranasal Administration ◽  
Blood Concentrations

Clearance of rabbit plasma angiotensinogen and relationship to CSF angiotensinogen

1983 ◽  
Vol 244 (4) ◽  
pp. H577-H585
Author(s):  
J. A. Lewicki ◽  
J. M. Printz ◽  
M. P. Printz
Keyword(s):  
Cerebrospinal Fluid ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Component Model ◽  
Activity Assay ◽  
Rabbit Plasma ◽  
Plasma Pool ◽  
The Central Nervous System ◽  
Clearance Kinetics ◽  
The Brain

Rabbit plasma angiotensinogen was purified 1,390-fold by classical purification procedures. Analytical polyacrylamide gel electrophoresis and direct activity assay revealed that the purified preparations were greater than 90% native angiotensinogen. The purified angiotensinogen was radiolabeled with 125I-Na using the immobilized lactoperoxidase-Sepharose method and injected into awake, conscious rabbits. Complex clearance kinetics were observed that were resolved by a three-component model; half of the protein was cleared rapidly (t1/2 = 6 and 54 min), presumably reflecting mixing and redistribution, whereas half exhibited slow clearance kinetics (t1/2 = 8.58 h). The clearance kinetics were independent of the method of iodination and the isoelectric-point microheterogeneity of the protein. With knowledge of clearance kinetics we tested whether cerebrospinal fluid angiotensinogen could derive from the plasma pool. After injection of approximately 100 microCi 125I-angiotensinogen into the rabbit circulation, little 125I-angiotensinogen was detected in cerebrospinal fluid. Further, the brain space of 125I-angiotensinogen was identical to that of 125I-albumin, a protein that does not partition from plasma into the central nervous system. We conclude that the brain prohormone does not appear to be derived from the plasma pool.

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