scholarly journals A High Molar Extinction Coefficient Mono-Anthracenyl Bipyridyl Heteroleptic Ruthenium(II) Complex: Synthesis, Photophysical and Electrochemical Properties

Molecules ◽  
2011 ◽  
Vol 16 (6) ◽  
pp. 4615-4631 ◽  
Author(s):  
Adewale O. Adeloye ◽  
Peter A. Ajibade
Keyword(s):  
Electrochemical Properties ◽  
Extinction Coefficient ◽  
Molar Extinction Coefficient

Synthesis and Characterization of ROSA Dye - A Rhodamine B-type Fluorophore, Suitable for Bioconjugation and Fluorescence Studies in Live Cells

2019 ◽  
Vol 26 (10) ◽  
pp. 758-767
Author(s):  
Vicente Rubio ◽  
Vijaya Iragavarapu ◽  
Maciej J. Stawikowski
Keyword(s):  
Quantum Yield ◽  
Cancer Cells ◽  
Fluorescent Probe ◽  
Extinction Coefficient ◽  
Rhodamine B ◽  
Solid Phase ◽  
Total Yield ◽  
Molar Extinction Coefficient ◽  
Αvβ3 Integrin ◽  
Αvβ3 Integrins

Background: Herein we report the multigram-scale synthesis, characterization and application of a rhodamine B-based fluorophore (ROSA) suitable for fluorescent studies in biological applications. This fluorophore is devoid of rhodamine spirolactone formation and furthermore characterized by a high molar extinction coefficient (ϵ=87250 ± 1630 M-1cm-1) and quantum yield (φ) of 0.589 ± 0.070 in water. Reported here is also the application of ROSA towards synthesis of a ROSA-PEG-GRGDS-NH2 fluorescent probe suitable for live cell imaging of αvβ3 integrins for in vitro assays. Objective: The main objective of this study is to efficiently prepare rhodamine B derivative, devoid of spirolactone formation that would be suitable for bioconjugation and subsequent bioimaging. Methods: Rhodamine B was transformed into rhodamine B succinimide ester (RhoB-OSu) using N-hydroxysuccinimide. RhoB-OSu was further coupled to sarcosine to obtain rhodamine Bsarcosine dye (ROSA) in good yield. The ROSA dye was then coupled to a αvβ3 integrin binding sequence using standard solid-phase conditions. Resulting ROSA-PEG-GRGDS-NH2 probe was used to image integrins on cancer cells. Results: The rhodamine B-sarcosine dye (ROSA) was obtained in multigram scale in good total yield of 47%. Unlike rhodamine B, the ROSA dye does not undergo pH-dependent spirolactone/spirolactam formation as compared with rhodamine B-glycine. It is also characterized by excellent quantum yield (φ) of 0.589 ± 0.070 in water and high molar extinction coefficient of 87250 ± 1630 M-1cm-1. ROSA coupling to the RGD-like peptide was proved to be efficient and straightforward. Imaging using standard filters on multimode plate reader and confocal microscope was performed. The αvβ3 integrins present on the surface of live WM-266-4 (melanoma) and MCF- 7 (breast cancer) cells were successfully imaged. Conclusion: We successfully derivatized rhodamine B to create an inexpensive, stable and convenient to use fluorescent probe. The obtained derivative has excellent photochemical properties and it is suitable for bioconjugation and many imaging applications.

scholarly journals Experimental Determination of the Absorption Cross-Section and Molar Extinction Coefficient of Colloidal CdSe Nanoplatelets

2015 ◽  
Vol 119 (47) ◽  
pp. 26768-26775 ◽  
Author(s):  
Aydan Yeltik ◽  
Savas Delikanli ◽  
Murat Olutas ◽  
Yusuf Kelestemur ◽  
Burak Guzelturk ◽  
...  
Keyword(s):  
Experimental Determination ◽  
Cross Section ◽  
Extinction Coefficient ◽  
Absorption Cross Section ◽  
Molar Extinction Coefficient ◽  
Absorption Cross

Extinction Coefficient of Plasmonic Nickel Sulfide Nanocrystals and Gold-Nickel Sulfide Core-Shell Nanoparticles

2018 ◽  
Vol 233 (1) ◽  
pp. 3-14 ◽  
Author(s):  
Rasmus Himstedt ◽  
Dominik Hinrichs ◽  
Dirk Dorfs
Keyword(s):  
Extinction Coefficient ◽  
Nickel Sulfide ◽  
Molar Extinction Coefficient ◽  
Localized Surface Plasmon ◽  
Core Shell ◽  
Electromagnetic Spectrum ◽  
Shell Nanoparticles ◽  
Extinction Coefficients ◽  
Optical Applications ◽  
Core Shell Nanoparticles

Abstract In the presented work, the molar extinction coefficient of plasmonic heazlewoodite (Ni3S2) nanoparticles and Au-Ni3S2 core-shell nanoparticles is determined for the first time. The results are compared to analogously determined extinction coefficients of pure Au nanocrystals (NCs), which themselves correlate very well with existing literature on the subject. The measured extinction coefficients at the localized surface plasmon resonance (LSPR) maximum wavelength of nickel sulfide particles are similar to the values of equally sized Au NCs. Therefore, considering the lower cost of the heazlewoodite material, it could be a reasonable alternative for optical applications of nanoparticles showing a LSPR in the visible regime of the electromagnetic spectrum. Furthermore, this study shows, that by growing a Ni3S2 shell onto a pure Au nanocrystal a highly tuneable optical material with variable LSPR frequency and molar extinction coefficient is obtained.

scholarly journals Plausible Applicability of Aqueous Congo Red Dye System as Secondary Gamma-ray Dosimeter

2008 ◽  
Vol 5 (3) ◽  
pp. 572-576
Author(s):  
I. Das Sarma ◽  
D. V. Parwate
Keyword(s):  
Extinction Coefficient ◽  
Congo Red ◽  
Gamma Ray ◽  
Molar Extinction Coefficient ◽  
Aqueous Systems ◽  
Γ Irradiation ◽  
Gamma Source ◽  
Congo Red Dye ◽  
Red Dye ◽  
Dye Solutions

Aqueous congo red dye solutions in the concentration range of 0.01–0.03mM were irradiated for varying γ doses 0.050 kGy to 0.150 kGy. The molar extinction coefficient of the dye solution of congo red was found to be 2.70×105at its absorption maxima 499.0 nm. No change in λmaxwas observed on irradiation. As the dye solutions are sensitive towards γ–irradiation, decolouration occurs on irradiation. The G–values for the doses in between the range of 0.035 kGy to 0.125 kGy were found to be practically constant for pure aqueous systems. Hence this was used to determine the dose of the gamma source. With the addition of alcohols in the systems, degradation of the dyes due to radiations decreased considerably.

scholarly journals Substitution of Carbazole Modified Fluorenes as π-Extension in Ru(II) Complex-Influence on Performance of Dye-Sensitized Solar Cells

2011 ◽  
Vol 2011 ◽  
pp. 1-10 ◽  
Author(s):  
Malapaka Chandrasekharam ◽  
Ganugula Rajkumar ◽  
Thogoti Suresh ◽  
Chikkam Srinivasa Rao ◽  
Paidi Yella Reddy ◽  
...  
Keyword(s):  
Dicarboxylic Acid ◽  
Extinction Coefficient ◽  
Density Functional ◽  
Fill Factor ◽  
Spectral Response ◽  
Short Circuit ◽  
Dye Sensitized Solar Cells ◽  
Molar Extinction Coefficient ◽  
Tert Butyl ◽  
Film Absorption

A new high molar extinction coefficient ruthenium(II) bipyridyl complex “cis-Ru(4,-bis(9,9-dibutyl-7-(3,6-di-tert-butyl-9H-carbazol-9-yl)-9H-fluoren-2-yl)-2,-bipyridine)(2,-bipyridine-4,-dicarboxylic acid)(NCS)2, BPFC” has been synthesized and characterized by FT-IR, -NMR, and ESI-MASS spectroscopes. The sensitizer showed molar extinction coefficient of  M−1cm−1, larger as compared to the reference N719, which showed  M−1cm−1. The test cells fabricated using BPFC sensitizer employing high performance volatile electrolyte, (E01) containing 0.05 M I2, 0.1 M LiI, 0.6 M 1,2-dimethyl-3-n-propylimidazolium iodide, 0.5 M 4-tert-butylpyridine in acetonitrile solvent, exhibited solar-to-electric energy conversion efficiency (η) of 4.65% (short-circuit current density () = 11.52 mA/cm2, open-circuit voltage () = 566 mV, fill factor = 0.72) under Air Mass 1.5 sunlight, lower as compared to the reference N719 sensitized solar cell, fabricated under similar conditions, which exhibited η-value of 6.5% ( = 14.3 mA/cm2, = 640 mV, fill factor = 0.71). UV-Vis measurements conducted on TiO2 films showed decreased film absorption ratios for BPFC as compared to those of reference N719. Staining TiO2 electrodes immediately after sonication of dye solutions enhanced film absorption ratios of BPFC relative to those of N719. Time-dependent density functional theory (TD-DFT) calculations show higher oscillation strengths for 4,-bis(9,9-dibutyl-7-(3,6-di-tert-butyl-9H-carbazol-9-yl)-9H-fluoren-2-yl)-2,-bipyridine relative to 2,-bipyridine-4,-dicarboxylic acid and increased spectral response for the corresponding BPFC complex.

Solvatation des électrons en excès dans les solvants polaires: corrélation entre les valeurs de εmax, W1/2 et EAmax

1990 ◽  
Vol 68 (4) ◽  
pp. 553-557 ◽  
Author(s):  
J.-P. Jay-Gerin ◽  
C. Ferradini
Keyword(s):  
Optical Absorption ◽  
Absorption Spectra ◽  
Extinction Coefficient ◽  
Molar Extinction Coefficient ◽  
Optical Absorption Spectra ◽  
Solvated Electron ◽  
Solvated Electrons ◽  
Half Height

On the basis of data found in the literature, it is shown that a correlation exists between the molar extinction coefficient at the maximum optical absorption of the solvated electron (εmax), its width at half height (W1/2), and the energy corresponding to that maximum (EAmax) Keywords: solvated electrons, polar solvants, optical absorption spectra. [Journal translation]

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