scholarly journals Antibacterial Effects of the Essential Oils of Commonly Consumed Medicinal Herbs Using an In Vitro Model

Molecules ◽  
2010 ◽  
Vol 15 (11) ◽  
pp. 7532-7546 ◽  
Author(s):  
Marina Soković ◽  
Jasmina Glamočlija ◽  
Petar D. Marin ◽  
Dejan Brkić ◽  
Leo J. L. D. van Griensven
Keyword(s):  
Essential Oils ◽  
In Vitro Model ◽  
Medicinal Herbs ◽  
Antibacterial Effects ◽  
Vitro Model

scholarly journals Corrigendum: Stojkovic D, Glamoclija J, Ciric A, Nikolic M, Ristic M, Siljegovic J, Sokovic M. Investigation on antibacterial synergism of Origanum vulgare and Thymus vulgaris essential oils. Arch biol sci. 2013;65(2):639-43. DOI: 10.2298/ABS1302639S

2015 ◽  
Vol 67 (4) ◽  
pp. 1441-1441
Author(s):  
E Editorial
Keyword(s):  
Essential Oils ◽  
In Vitro Model ◽  
Medicinal Herbs ◽  
Thymus Vulgaris ◽  
Antibacterial Effects ◽  
Origanum Vulgare ◽  
Vitro Model ◽  
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The authors of the above-referenced article have requested a corrigendum to it. The article should include the following reference: Sokovic, M., Glamoclija, J., Marin, D.P., Brkic, D., and L. J. L. D. van Griensven (2010) Antibacterial Effects of the Essential Oils of Commonly Consumed Medicinal Herbs Using an In Vitro Model. Molecules. 15(11), 7532-7546. <br><br><font color="red"><b> Link to the corrected article <u><a href="http://dx.doi.org/10.2298/ABS1302639S">10.2298/ABS1302639S</a></b></u>

scholarly journals Antimicrobial Effects of Essential Oils on Oral Microbiota Biofilms: The Toothbrush In Vitro Model

Antibiotics ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 21
Author(s):  
Andreia Aires ◽  
António Salvador Barreto ◽  
Teresa Semedo-Lemsaddek
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
In Vitro Model ◽  
Inhibitory Concentration ◽  
Oral Bacteria ◽  
Bacterial Biofilm ◽  
Antimicrobial Effects ◽  
Vitro Model ◽  
Biofilm Development

The present investigation intended to evaluate the bacteriostatic and bactericidal abilities of clove, oregano and thyme essential oils against oral bacteria in planktonic and biofilm states. Furthermore, aiming to mimic everyday conditions, a toothbrush in vitro model was developed. Determination of the minimum inhibitory concentration, minimum bactericidal concentration, minimum biofilm inhibitory concentration and minimum biofilm eradication concentration were achieved using the microdilution procedure. To simulate the toothbrush environment, nylon fibers were inoculated with oral bacteria, which, after incubation to allow biofilm development, were submitted to contact with the essential oils under study. Thyme and oregano essential oils revealed promising antimicrobial effects, both in growth inhibition and the destruction of cells in planktonic and biofilm states, while clove essential oil showed a weaker potential. Regarding the toothbrush in vitro model, observation of the nylon fibers under a magnifying glass proved the essential oil anti-biofilm properties. Considering the effects observed using the in vitro toothbrush model, a realistic approximation to oral biofilm establishment in an everyday use object, a putative application of essential oils as toothbrush sanitizers to help prevent the establishment of bacterial biofilm can be verified.

scholarly journals Essential oils of Bursera morelensis and Lippia graveolens for the development of a new biopesticides in postharvest control

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Yoli Mariana Medina-Romero ◽  
Ana Bertha Hernandez-Hernandez ◽  
Marco Aurelio Rodriguez-Monroy ◽  
María Margarita Canales-Martínez
Keyword(s):  
Antifungal Activity ◽  
Essential Oils ◽  
In Vitro Model ◽  
Gas Chromatography Mass Spectrometry ◽  
Vegetable Crops ◽  
Environment Friendly ◽  
Plant Model ◽  
Vitro Model ◽  
Phytopathogenic Microorganisms

AbstractFruit and vegetable crops that are not consumed immediately, unlike other agricultural products, require economic and time investments until they reach the final consumers. Synthetic agrochemicals are used to maintain and prolong the storage life of crops and avoid losses caused by phytopathogenic microorganisms. However, the excessive use of synthetic agrochemicals creates health problems and contributes to environmental pollution. To avoid these problems, less toxic and environment-friendly alternatives are sought. One of these alternatives is the application of biopesticides. However, few biopesticides are currently used. In this study, the biopesticide activity of Bursera morelensis and Lippia graveolens essential oils was evaluated. Their antifungal activity has been verified in an in vitro model, and chemical composition has been determined using gas chromatography-mass spectrometry. Their antifungal activity was corroborated in vitro, and their activity as biopesticides was subsequently evaluated in a plant model. In addition, the persistence of these essential oils on the surface of the plant model was determined. Results suggest that both essential oils are promising candidates for producing biopesticides. This is the first study showing that B. morelensis and L. graveolens essential oils work by inhibiting mycelial growth and spore germination and are environment-friendly biopesticides.

An in vitro model for investigation of vitamin A effects on wound healing

2021 ◽  
pp. 1-6
Author(s):  
Hoda Keshmiri Neghab ◽  
Mohammad Hasan Soheilifar ◽  
Gholamreza Esmaeeli Djavid
Keyword(s):  
Wound Healing ◽  
Endothelial Cell ◽  
Vitamin A ◽  
In Vitro Model ◽  
Cellular Proliferation ◽  
Endothelial Cell Migration ◽  
Normal Fibroblast ◽  
Anti Inflammatory ◽  
Vitro Model

Abstract. Wound healing consists of a series of highly orderly overlapping processes characterized by hemostasis, inflammation, proliferation, and remodeling. Prolongation or interruption in each phase can lead to delayed wound healing or a non-healing chronic wound. Vitamin A is a crucial nutrient that is most beneficial for the health of the skin. The present study was undertaken to determine the effect of vitamin A on regeneration, angiogenesis, and inflammation characteristics in an in vitro model system during wound healing. For this purpose, mouse skin normal fibroblast (L929), human umbilical vein endothelial cell (HUVEC), and monocyte/macrophage-like cell line (RAW 264.7) were considered to evaluate proliferation, angiogenesis, and anti-inflammatory responses, respectively. Vitamin A (0.1–5 μM) increased cellular proliferation of L929 and HUVEC (p < 0.05). Similarly, it stimulated angiogenesis by promoting endothelial cell migration up to approximately 4 fold and interestingly tube formation up to 8.5 fold (p < 0.01). Furthermore, vitamin A treatment was shown to decrease the level of nitric oxide production in a dose-dependent effect (p < 0.05), exhibiting the anti-inflammatory property of vitamin A in accelerating wound healing. These results may reveal the therapeutic potential of vitamin A in diabetic wound healing by stimulating regeneration, angiogenesis, and anti-inflammation responses.

A new in vitro model for pre-transplantation diagnosis of frozen/thawed human ovarian tissue

2011 ◽  
Vol 71 (05) ◽  
Author(s):  
M Salama ◽  
K Winkler ◽  
KF Murach ◽  
S Hofer ◽  
L Wildt ◽  
...  
Keyword(s):  
In Vitro Model ◽  
Ovarian Tissue ◽  
Human Ovarian Tissue ◽  
Vitro Model
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