scholarly journals Corrigendum: Stojkovic D, Glamoclija J, Ciric A, Nikolic M, Ristic M, Siljegovic J, Sokovic M. Investigation on antibacterial synergism of Origanum vulgare and Thymus vulgaris essential oils. Arch biol sci. 2013;65(2):639-43. DOI: 10.2298/ABS1302639S

2015 ◽  
Vol 67 (4) ◽  
pp. 1441-1441
Author(s):  
E Editorial
Keyword(s):  
Essential Oils ◽  
In Vitro Model ◽  
Medicinal Herbs ◽  
Thymus Vulgaris ◽  
Antibacterial Effects ◽  
Origanum Vulgare ◽  
Vitro Model ◽  
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The authors of the above-referenced article have requested a corrigendum to it. The article should include the following reference: Sokovic, M., Glamoclija, J., Marin, D.P., Brkic, D., and L. J. L. D. van Griensven (2010) Antibacterial Effects of the Essential Oils of Commonly Consumed Medicinal Herbs Using an In Vitro Model. Molecules. 15(11), 7532-7546. <br><br><font color="red"><b> Link to the corrected article <u><a href="http://dx.doi.org/10.2298/ABS1302639S">10.2298/ABS1302639S</a></b></u>

scholarly journals Antibacterial Effects of the Essential Oils of Commonly Consumed Medicinal Herbs Using an In Vitro Model

Molecules ◽  
2010 ◽  
Vol 15 (11) ◽  
pp. 7532-7546 ◽  
Author(s):  
Marina Soković ◽  
Jasmina Glamočlija ◽  
Petar D. Marin ◽  
Dejan Brkić ◽  
Leo J. L. D. van Griensven
Keyword(s):  
Essential Oils ◽  
In Vitro Model ◽  
Medicinal Herbs ◽  
Antibacterial Effects ◽  
Vitro Model

scholarly journals Chemical composition and in vitro activity of Origanum vulgare L., Satureja hortensis L., Thymus serpyllum L. and Thymus vulgaris L. essential oils towards oral isolates of Candida albicans and Candida glabrata

2020 ◽  
Vol 18 (1) ◽  
pp. 108-118 ◽  
Author(s):  
Tomasz Baj ◽  
Anna Biernasiuk ◽  
Rafał Wróbel ◽  
Anna Malm
Keyword(s):  
Candida Albicans ◽  
Chemical Composition ◽  
Antifungal Activity ◽  
Essential Oils ◽  
Thymus Vulgaris ◽  
Origanum Vulgare ◽  
Satureja Hortensis ◽  
Broth Microdilution Method ◽  
Thymus Serpyllum

AbstractThe purpose of this research was to investigate the chemical composition of essential oils (EOs) from: Origanum vulgare L., Satureja hortensis L., Thymus serpyllum L. and Thymus vulgaris L. (Lamiaceae) cultivated in Poland, and to study their antifungal activity towards clinical isolates of oral Candida spp. The hydrodistilled essential oils were analyzed using the GC-MS method. The antifungal activity was evaluated in vitro against oral isolates and reference strains of Candida albicans and C. glabrata, using the broth microdilution method according to the European Committee on Antimicrobial Susceptibility Testing (EUCAST) and Clinical and Laboratory Standards Institute (CLSI) guidelines, allowing for estimation of minimal inhibitory concentration (MIC) and minimal fungicidal concentration (MFC). GC-MS analysis revealed that carvacrol was the main EO compound in oregano and summer savory, while thymol and linalool were the major ingredients of thyme and wild thyme oils, respectively. The EOs possessed fungicidal activity against C. albicans and C. glabrata, including oral isolates, with MIC = 125 – 2000 mg/L, MFC = 250 – 4000 mg/L and MFC/MIC = 1 – 4, depending on the yeast and plant species. The most active was thyme oil – with MIC = 125 – 500 mg/L, MFC = 250 – 500 mg/L and MFC/MIC = 1 – 2.

scholarly journals Validation of Qualitative Broth Volatilization Checkerboard Method for Testing of Essential Oils: Dual-Column GC–FID/MS Analysis and In Vitro Combinatory Antimicrobial Effect of Origanum vulgare and Thymus vulgaris against Staphylococcus aureus in Liquid and Vapor Phases

Plants ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 393
Author(s):  
Marie Netopilova ◽  
Marketa Houdkova ◽  
Klara Urbanova ◽  
Johana Rondevaldova ◽  
Ladislav Kokoska
Keyword(s):  
Staphylococcus Aureus ◽  
Essential Oils ◽  
Vapor Phase ◽  
Antimicrobial Agents ◽  
Gas Chromatography Mass Spectrometry ◽  
Thymus Vulgaris ◽  
Origanum Vulgare ◽  
Dual Column ◽  
Liquid And Vapor Phases

Combinatory action of antimicrobial agents such as essential oils (EOs) show to be an effective strategy to overcome the problem with increasing antibiotic resistance of microorganisms, including Staphylococcus aureus. The objective of this study was to evaluate in vitro antimicrobial interactions between Origanum vulgare and Thymus vulgaris EOs against various S.aureus strains in both liquid and vapor phases using the broth volatilization checkerboard method. Fractional inhibitory concentrations (FICs) were determined for both liquid and vapor phases, and the composition of EOs was analyzed by gas chromatography-mass spectrometry using dual-column/dual-detector gas chromatograph. Results of oregano and thyme EOs combination showed additive effects against all S. aureus strains in both phases. In several cases, sums of FICs were lower than 0.6, which can be considered a strong additive interaction. The lowest FICs obtained were 0.53 in the liquid phase and 0.59 in the gaseous phase. Chemical analysis showed that both EOs were composed of many compounds, including carvacrol, thymol, γ-terpinene, and p-cymene. This is the first report on oregano and thyme EOs interactions against S. aureus in the vapor phase. It also confirms the accuracy of the broth volatilization checkerboard method for the evaluation of combinatory antimicrobial effects of EOs in the vapor phase.

scholarly journals Antimicrobial activity of thyme (Thymus vulgaris) and oregano (Origanum vulgare) essential oils against Listeria monocytogenes in fermented sausages

2021 ◽  
Vol 854 (1) ◽  
pp. 012064
Author(s):  
Drago Nedic ◽  
Nevena Grkovic ◽  
Vesna Kalaba ◽  
Bojan Golic ◽  
Tanja Ilic ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Essential Oil ◽  
Essential Oils ◽  
Detection Threshold ◽  
Thymus Vulgaris ◽  
Antibacterial Effects ◽  
High Concentration ◽  
Origanum Vulgare ◽  
Fermented Sausages ◽  
Microbiological Status

Abstract The aim of this study was to investigate antibacterial effects of oregano and thyme essential oils on Listeria monocytogenes in fermented sausages and their effect on the sensory characteristics of these sausages. For testing purposes, sausages contaminated with L. monocytogenes were produced. Changes in the microbiological status of fermented sausages and physicochemical properties were monitored during ripening. Essential oils exhibited antibacterial activity against L. monocytogenes, and in the groups with a high concentration (0.6%) of oregano or thyme essential oils (KLO2 and KLT2), the number of L. monocytogenes was below the detection threshold on day 14 of ripening, with a stronger effect of oregano. In groups with 0.3% essential oil of oregano or thyme added, the number of L. monocytogenes was reduced to below the detection threshold on day 21 of ripening. During the ripening, the aw and pH of all test groups of fermented sausages decreased. Experimental sausages with 0.3% thyme essential oil had acceptable smell and taste, while in other experimental groups, sausage smell and taste were very intense, uncharacteristic and unacceptable.

scholarly journals Antimicrobial Effects of Essential Oils on Oral Microbiota Biofilms: The Toothbrush In Vitro Model

Antibiotics ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 21
Author(s):  
Andreia Aires ◽  
António Salvador Barreto ◽  
Teresa Semedo-Lemsaddek
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
In Vitro Model ◽  
Inhibitory Concentration ◽  
Oral Bacteria ◽  
Bacterial Biofilm ◽  
Antimicrobial Effects ◽  
Vitro Model ◽  
Biofilm Development

The present investigation intended to evaluate the bacteriostatic and bactericidal abilities of clove, oregano and thyme essential oils against oral bacteria in planktonic and biofilm states. Furthermore, aiming to mimic everyday conditions, a toothbrush in vitro model was developed. Determination of the minimum inhibitory concentration, minimum bactericidal concentration, minimum biofilm inhibitory concentration and minimum biofilm eradication concentration were achieved using the microdilution procedure. To simulate the toothbrush environment, nylon fibers were inoculated with oral bacteria, which, after incubation to allow biofilm development, were submitted to contact with the essential oils under study. Thyme and oregano essential oils revealed promising antimicrobial effects, both in growth inhibition and the destruction of cells in planktonic and biofilm states, while clove essential oil showed a weaker potential. Regarding the toothbrush in vitro model, observation of the nylon fibers under a magnifying glass proved the essential oil anti-biofilm properties. Considering the effects observed using the in vitro toothbrush model, a realistic approximation to oral biofilm establishment in an everyday use object, a putative application of essential oils as toothbrush sanitizers to help prevent the establishment of bacterial biofilm can be verified.

scholarly journals The Antifungal Properties of Super-Hydrophobic Nanoparticles and Essential Oils on Different Material Surfaces

Coatings ◽  
2019 ◽  
Vol 9 (3) ◽  
pp. 176 ◽  
Author(s):  
Monika Benkovičová ◽  
Zuzana Kisová ◽  
Mária Bučková ◽  
Eva Majková ◽  
Peter Šiffalovič ◽  
...  
Keyword(s):  
Essential Oils ◽  
Thuja Plicata ◽  
Thymus Vulgaris ◽  
Digital Microscopy ◽  
Mold Growth ◽  
Origanum Vulgare ◽  
Protective Properties ◽  
Color Changes ◽  
Material Surfaces

This study was undertaken to determine the in vitro antifungal activities of super-hydrophobic nanoparticles (SHNPs), essential oils (EOs), and their mixtures (SHNPs/EOs). We have applied a thin layer of SHNPs in combination with various concentrations of three EOs: Arborvitae (Thuja plicata), Oregano (Origanum vulgare L.), and Thyme (Thymus vulgaris). The mixtures were spread on the surface of different materials: whitewood, sandstone, and paper. The antifungal and protective properties of these SHNP and EO mixtures were evaluated. The parameter Rr (ratio of reflectivity) was determined to identify the color changes of substrates. Digital microscopy was used to measure the colonization area of molds and also their penetration in the analyzed materials. Surprisingly, the use of SHNPs alone showed a balanced compromise in order to inhibit the mold growth on assayed surfaces.

scholarly journals Essential oils of Bursera morelensis and Lippia graveolens for the development of a new biopesticides in postharvest control

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Yoli Mariana Medina-Romero ◽  
Ana Bertha Hernandez-Hernandez ◽  
Marco Aurelio Rodriguez-Monroy ◽  
María Margarita Canales-Martínez
Keyword(s):  
Antifungal Activity ◽  
Essential Oils ◽  
In Vitro Model ◽  
Gas Chromatography Mass Spectrometry ◽  
Vegetable Crops ◽  
Environment Friendly ◽  
Plant Model ◽  
Vitro Model ◽  
Phytopathogenic Microorganisms

AbstractFruit and vegetable crops that are not consumed immediately, unlike other agricultural products, require economic and time investments until they reach the final consumers. Synthetic agrochemicals are used to maintain and prolong the storage life of crops and avoid losses caused by phytopathogenic microorganisms. However, the excessive use of synthetic agrochemicals creates health problems and contributes to environmental pollution. To avoid these problems, less toxic and environment-friendly alternatives are sought. One of these alternatives is the application of biopesticides. However, few biopesticides are currently used. In this study, the biopesticide activity of Bursera morelensis and Lippia graveolens essential oils was evaluated. Their antifungal activity has been verified in an in vitro model, and chemical composition has been determined using gas chromatography-mass spectrometry. Their antifungal activity was corroborated in vitro, and their activity as biopesticides was subsequently evaluated in a plant model. In addition, the persistence of these essential oils on the surface of the plant model was determined. Results suggest that both essential oils are promising candidates for producing biopesticides. This is the first study showing that B. morelensis and L. graveolens essential oils work by inhibiting mycelial growth and spore germination and are environment-friendly biopesticides.

An in vitro model for investigation of vitamin A effects on wound healing

2021 ◽  
pp. 1-6
Author(s):  
Hoda Keshmiri Neghab ◽  
Mohammad Hasan Soheilifar ◽  
Gholamreza Esmaeeli Djavid
Keyword(s):  
Wound Healing ◽  
Endothelial Cell ◽  
Vitamin A ◽  
In Vitro Model ◽  
Cellular Proliferation ◽  
Endothelial Cell Migration ◽  
Normal Fibroblast ◽  
Anti Inflammatory ◽  
Vitro Model

Abstract. Wound healing consists of a series of highly orderly overlapping processes characterized by hemostasis, inflammation, proliferation, and remodeling. Prolongation or interruption in each phase can lead to delayed wound healing or a non-healing chronic wound. Vitamin A is a crucial nutrient that is most beneficial for the health of the skin. The present study was undertaken to determine the effect of vitamin A on regeneration, angiogenesis, and inflammation characteristics in an in vitro model system during wound healing. For this purpose, mouse skin normal fibroblast (L929), human umbilical vein endothelial cell (HUVEC), and monocyte/macrophage-like cell line (RAW 264.7) were considered to evaluate proliferation, angiogenesis, and anti-inflammatory responses, respectively. Vitamin A (0.1–5 μM) increased cellular proliferation of L929 and HUVEC (p < 0.05). Similarly, it stimulated angiogenesis by promoting endothelial cell migration up to approximately 4 fold and interestingly tube formation up to 8.5 fold (p < 0.01). Furthermore, vitamin A treatment was shown to decrease the level of nitric oxide production in a dose-dependent effect (p < 0.05), exhibiting the anti-inflammatory property of vitamin A in accelerating wound healing. These results may reveal the therapeutic potential of vitamin A in diabetic wound healing by stimulating regeneration, angiogenesis, and anti-inflammation responses.

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