scholarly journals Changes in Skin and Nasal Microbiome and Staphylococcal Species Following Treatment of Atopic Dermatitis with Dupilumab

2021 ◽  
Vol 9 (7) ◽  
pp. 1487
Author(s):  
Caroline Meyer Olesen ◽  
Anna Cäcilia Ingham ◽  
Simon Francis Thomsen ◽  
Maja-Lisa Clausen ◽  
Paal Skytt Andersen ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Clinical Improvement ◽  
Bacterial Community Structure ◽  
Gene Sequencing ◽  
Skin Microbiome ◽  
Sequencing Data ◽  
Tuf Gene ◽  
Targeted Treatments ◽  
Before And After

Investigation of changes in the skin microbiome following treatment of atopic dermatitis (AD) with dupilumab may provide valuable insights into the skin microbiome as a therapeutic target. The aim of this study is to assess changes in the AD skin microbiome following treatment of AD with dupilumab (n = 27). E-swabs were collected from nose, lesional, and nonlesional skin before and after 16 weeks of dupilumab therapy, and the microbiome was analyzed by 16S rRNA and tuf gene sequencing. Data for 17 patients with milder disease receiving treatment with non-targeted therapies are also presented. The results show that both groups experienced clinical improvement (p < 0.001) following dupilumab therapy and that Shannon diversity increased and bacterial community structure changed. The relative abundance of the genus Staphylococcus (S.) and S. aureus decreased, while that of S. epidermidis and S. hominis increased. No significant changes were observed for patients receiving non-targeted treatments. The increases in S. epidermidis and S. hominis and the decrease in S. aureus correlated with clinical improvement. Furthermore, changes in S. hominis and S. epidermidis correlated inversely with S. aureus. In conclusion, treatment with dupilumab significantly changed the skin microbiome and decreased S. aureus. Our results suggest a favorable role of commensal staphylococci in AD.

scholarly journals The role of the skin microbiome in atopic dermatitis

2019 ◽  
Vol 122 (3) ◽  
pp. 263-269 ◽  
Author(s):  
Teruaki Nakatsuji ◽  
Richard L. Gallo
Keyword(s):  
Atopic Dermatitis ◽  
Skin Microbiome

scholarly journals The role of the skin microbiome in atopic dermatitis: a systematic review

2017 ◽  
Vol 177 (5) ◽  
pp. 1272-1278 ◽  
Author(s):  
R.D. Bjerre ◽  
J. Bandier ◽  
L. Skov ◽  
L. Engstrand ◽  
J.D. Johansen
Keyword(s):  
Systematic Review ◽  
Atopic Dermatitis ◽  
Skin Microbiome

scholarly journals Alteration of Bacterial Communities in Anterior Nares and Skin Sites of Patients Undergoing Arthroplasty Surgery: Analysis by 16S rRNA and Staphylococcal-Specific tuf Gene Sequencing

2020 ◽  
Author(s):  
Søren Iversen ◽  
Thor Bech Johannesen ◽  
Anna Cäcilia Ingham ◽  
Sofie Marie Edslev ◽  
Staffan Tevell ◽  
...  
Keyword(s):  
16S Rrna ◽  
Bacterial Communities ◽  
Gene Sequencing ◽  
Alpha Diversity ◽  
Clinical Samples ◽  
Staphylococcal Species ◽  
Tuf Gene ◽  
Before And After ◽  
The Impact ◽  
Arthroplasty Surgery

The aim was to study alterations of bacterial communities in patients undergoing hip or knee arthroplasty to assess the impact of chlorhexidine gluconate soap decolonisation and systemic antibiotic prophylaxis. A Swedish multicentre, prospective collection of samples obtained from elective arthroplasty patients (n=83) by swabbing anterior nares, skin sites in the groin and the site of planned surgery, before and after arthroplasty surgery, was analysed by 16S rRNA (V3-V4) gene sequencing and a complementary targeted tuf gene sequencing approach to comprehensively characterise alterations in staphylococcal communities. Significant reductions in alpha diversity was detected for both bacterial (p=0.04) and staphylococcal (p=0.03) groin communities after arthroplasty surgery with significant reductions in relative Corynebacterium (p=0.001) abundance and S. hominis (p=0.01) relative staphylococcal abundance. In nares, significant reductions occurred for S. hominis (p=0.02), S. haemolyticus (p=0.02), and S. pasteuri (p=0.003) relative to other staphylococci. S. aureus colonised 35% of anterior nares before and 26% after arthroplasty surgery. S. epidermidis was the most abundant staphylococcal species at all sampling sites. No bacterial genus or staphylococcal species increased significantly after arthroplasty surgery. Application of a targeted tuf gene sequencing approach provided auxiliary staphylococcal community profiles and allowed species-level characterisation directly from low biomass clinical samples.

Skin microbiomein adult atopic dermatitis

2018 ◽  
pp. 209-214
Author(s):  
О.В. Кандалова ◽  
И.В. Елистратова ◽  
О.Б. Иванченко ◽  
А.В. Гречко ◽  
С.Г. Морозов
Keyword(s):  
Atopic Dermatitis ◽  
Environmental Factors ◽  
Bacterial Infection ◽  
Adaptive Immunity ◽  
Bacterial Contamination ◽  
Proteolytic Enzymes ◽  
Regulatory Proteins ◽  
Skin Microbiome ◽  
Innate And Adaptive Immunity

Данный миниобзор посвящен изучению роли микробиома кожи и, в частности, роли стафилококков в обострении атопического дерматита у взрослых людей. Были проанализированы предпосылки бактериальной контаминации кожи и роль факторов внешней среды. Представлены данные по влиянию S. aureaus на разные звенья природного и адаптивного иммунитета за счет синтеза специфических регуляторных белков, протеолитических ферментов и гидролаз. Обозначены некоторые направления борьбы с бактериальной инфекцией для предупреждения обострения атопического дерматита у взрослых. In this mini review we have analyzed the role of skin microbiome in the atopic dermatitis relapse in adults, in particular, a role of S. aureaus in this process. The background for the skin bacterial contamination under the influence of environmental factors has been analyzed. We reviewed some S. aureaus effects on the components of innate and adaptive immunity due to the secretion of specific regulatory proteins, a number of proteolytic enzymes, and some hydrolases. There were indicated some ways to eliminate the bacterial infection to prevent the atopic dermatitis relapse in adults

The role of skin microbiome in atopic dermatitis

2017 ◽  
Vol 93 (5) ◽  
pp. 202-208
Author(s):  
László Hunor Gergely ◽  
◽  
Miklós Sárdy ◽  
Noémi Muhalik
Keyword(s):  
Atopic Dermatitis ◽  
Skin Microbiome

The emerging role of skin microbiome in atopic dermatitis and its clinical implication

2018 ◽  
Vol 30 (4) ◽  
pp. 357-364 ◽  
Author(s):  
Jean-François Stalder ◽  
Joachim W. Fluhr ◽  
Tim Foster ◽  
Martin Glatz ◽  
Ehrhardt Proksch
Keyword(s):  
Atopic Dermatitis ◽  
Clinical Implication ◽  
Skin Microbiome

scholarly journals THE ROLE OF GENETIC POLYMORPHISM OF THE FILAGGRIN PROTEIN WITH ATOPIC MARCH PROGRESSION IN CHILDREN

InterConf ◽  
2021 ◽  
pp. 187-200
Author(s):  
Tetiana Stoieva ◽  
Olesia Reshetilo ◽  
Natalia Vesilyk ◽  
Olha Portnova ◽  
Maksym Fedin ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Genetic Polymorphism ◽  
Gene Polymorphism ◽  
Life Quality ◽  
House Dust Mites ◽  
Control Group ◽  
Skin Microbiome ◽  
Atopic March ◽  
The Impact

The aim of the study. To determine the role of genetic polymorphism in the filaggrin gene R501XAA and 2282de4AA at atopic march progression in children. Materials and methods. 111 children aged 3 to 12 years with atopic dermatitis were selected and examined. As a result of genetic testing, it was found that 51 children with atopic dermatitis had polymorphism in the filaggrin gene. These patients were included in the main group. Another 60 children without polymorphism were in the control group. The filaggrin gene polymorphism was determined by examining the buccal epithelium by Dellaporta method. Sensitization to allergens was established on the basis of the specific IgE level. The impact of the disease on the quality of life of children was performed using the CDLQI questionnaire (Children's Dermatology Life Quality Index). Results. In the course of molecular genetics research, R501X mutation was detected in 40 ((78.4 ± 5.76)%) children, 2282del4 polymorphism – in 4 ((7.8 ± 3.76)%) patients, and their combined variant R501X + 2282del4 – in 7 (13%), (7 ± 4.81)% patients. When determining the effect of filaggrin polymorphism on the clinical course of atopic dermatitis, the presence of the associative relationship was established with the following indicators: the early onset of the disease – χ2 = 33.2, mostly severe course – χ2 = 16.2, severe skin dryness – χ2 = 22.6, predominant sensitization to fungi – χ2 = 10.6 and house dust mites – χ2 = 12.2, violation of the skin microbiome – χ2 = 7.8. Conclusions. Early manifestation of atopic dermatitis in children is associated with the filaggrin protein gene polymorphism ((82.4 ± 5.33)%), which determines the risk of progression of the atopic march and the development of bronchial asthma in (38.0 ± 6.8)% of children.

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