scholarly journals Differential Exoproteome and Biochemical Characterisation of Neoparamoeba perurans

2021 ◽  
Vol 9 (6) ◽  
pp. 1258
Author(s):  
Kerrie Ní Ní Dhufaigh ◽  
Natasha Botwright ◽  
Eugene Dillon ◽  
Ian O’Connor ◽  
Eugene MacCarthy ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
Virulence Factors ◽  
Cytotoxic Effect ◽  
Differentially Expressed Proteins ◽  
Biochemical Characterisation ◽  
Key Players ◽  
Prolonged Culture ◽  
Global Threat ◽  
Iron Receptor ◽  
Trout Gill

Infection with the protozoan ectoparasite Neoparamoeba perurans, the causative agent of AGD, remains a global threat to salmonid farming. This study aimed to analyse the exoproteome of both an attenuated and virulent N. perurans isolate using proteomics and cytotoxicity testing. A disproportionate presence of proteins from the co-cultured microbiota of N. perurans was revealed on searching an amalgamated database of bacterial, N. perurans and Amoebozoa proteins. LC‑MS/MS identified 33 differentially expressed proteins, the majority of which were upregulated in the attenuated exoproteome. Proteins of putative interest found in both exoproteomes were maltoporin, ferrichrome-iron receptor, and putative ferric enterobactin receptor. Protease activity remained significantly elevated in the attenuated exoproteome compared with the virulent exoproteome. Similarly, the attenuated exoproteome had a significantly higher cytotoxic effect on rainbow trout gill cell line (RTgill W1) cells compared with the virulent exoproteome. The presence of a phosphatase and serine protease in the virulent exoproteome may facilitate AGD infection but do not appear to be key players in causing cytotoxicity. Altogether, this study reveals prolonged culture of N. perurans affects the exoproteome composition in favour of nutritional acquisition, and that the current culturing protocol for virulent N. perurans does not facilitate the secretion of virulence factors.

Responses of trout gill ion transport systems to acute acidosis

1976 ◽  
Vol 64 (2) ◽  
pp. 511-515
Author(s):  
T. H. Kerstetter ◽  
R. Mize
Keyword(s):  
Lactic Acid ◽  
Rainbow Trout ◽  
Ion Transport ◽  
Ion Uptake ◽  
Transport Systems ◽  
Ph Changes ◽  
Blood Ph ◽  
Slow Infusion ◽  
Trout Gill

The response of rainbow trout Na+ and Cl- uptake systems to acute acidosis was tested by slow infusion of lactic acid into anaesthetized animals. Depression of blood pH by 0–4 pH unit had no effect on influx rates for either ion, and we conclude that gill ion uptake systems do not respond rapidly to blood pH changes.

scholarly journals Challenge of drug resistance in Pseudomonas aeruginosa: clonal spread of NDM-1-positive ST-308 within a tertiary hospital

2019 ◽  
Vol 74 (8) ◽  
pp. 2220-2224 ◽  
Author(s):  
Ka Lip Chew ◽  
Sophie Octavia ◽  
Oon Tek Ng ◽  
Kalisvar Marimuthu ◽  
Indumathi Venkatachalam ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Drug Resistance ◽  
Virulence Factors ◽  
Acquired Resistance ◽  
Hospital Setting ◽  
Tertiary Hospital ◽  
Secretion System ◽  
Type Vi Secretion System ◽  
Genomic Epidemiology ◽  
Global Threat

Abstract Objectives MDR Pseudomonas aeruginosa is a serious global threat to healthcare institutions. The mechanism by which drug resistance can be acquired is variable, but acquired carbapenemase production has been reported in P. aeruginosa. An investigation was performed to determine the rate and genomic epidemiology of New Delhi MBL (NDM) in β-lactam-non-susceptible isolates. Methods P. aeruginosa isolates from a tertiary hospital in Singapore between January 2015 and February 2018 were investigated for the presence of NDM genes. Results Out of 298 pan-β-lactam-non-susceptible isolates, 31 were found to be NDM positive (10.4%). WGS demonstrated that all 31 NDM-positive isolates were clonal, belonging to ST-308. blaNDM was chromosomally inserted within an integrative and conjugative element (ICE), ICETn43716385. The NDM-P. aeruginosa isolates possessed an extensive repertoire of both cell-associated [flagella, pili, alginate/biofilm, LPS, type III secretion system (T3SS) and type VI secretion system (T6SS)] and secreted virulence factors. Antibiograms revealed higher rates of drug resistance in NDM-positive isolates compared with their non-NDM counterparts. The NDM isolates remained 100% susceptible only to colistin. Conclusions The combination of chromosomal mutations, acquired resistance genes and virulence factors likely facilitated the persistent and ongoing spread of the ST-308 clade of P. aeruginosa within the hospital. Our study illustrates the particular threat of NDM-positive P. aeruginosa in a tertiary hospital setting in the era of antimicrobial resistance.

Accumulation of hsp70 in juvenile and adult rainbow trout gill exposed to metal-contaminated water and/or diet

1996 ◽  
Vol 15 (8) ◽  
pp. 1324-1328 ◽  
Author(s):  
Jeanne H. Williams ◽  
Nancy S. Petersen ◽  
Patricia A. Young ◽  
Mark A. Stansbury ◽  
Aida M. Farag ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
Contaminated Water ◽  
Trout Gill

scholarly journals Gene expression profiling identifies novel key players involved in the cytotoxic effect of Artesunate on pancreatic cancer cells

2009 ◽  
Vol 78 (3) ◽  
pp. 273-283 ◽  
Author(s):  
Mahmoud Youns ◽  
Thomas Efferth ◽  
Jürgen Reichling ◽  
Kurt Fellenberg ◽  
Andrea Bauer ◽  
...  
Keyword(s):  
Gene Expression ◽  
Pancreatic Cancer ◽  
Gene Expression Profiling ◽  
Cancer Cells ◽  
Expression Profiling ◽  
Cytotoxic Effect ◽  
Pancreatic Cancer Cells ◽  
Key Players
Sign in / Sign up

Export Citation Format

Share Document