Accumulation of hsp70 in juvenile and adult rainbow trout gill exposed to metal-contaminated water and/or diet

1996 ◽  
Vol 15 (8) ◽  
pp. 1324-1328 ◽  
Author(s):  
Jeanne H. Williams ◽  
Nancy S. Petersen ◽  
Patricia A. Young ◽  
Mark A. Stansbury ◽  
Aida M. Farag ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
Contaminated Water ◽  
Trout Gill

scholarly journals Differential Exoproteome and Biochemical Characterisation of Neoparamoeba perurans

2021 ◽  
Vol 9 (6) ◽  
pp. 1258
Author(s):  
Kerrie Ní Ní Dhufaigh ◽  
Natasha Botwright ◽  
Eugene Dillon ◽  
Ian O’Connor ◽  
Eugene MacCarthy ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
Virulence Factors ◽  
Cytotoxic Effect ◽  
Differentially Expressed Proteins ◽  
Biochemical Characterisation ◽  
Key Players ◽  
Prolonged Culture ◽  
Global Threat ◽  
Iron Receptor ◽  
Trout Gill

Infection with the protozoan ectoparasite Neoparamoeba perurans, the causative agent of AGD, remains a global threat to salmonid farming. This study aimed to analyse the exoproteome of both an attenuated and virulent N. perurans isolate using proteomics and cytotoxicity testing. A disproportionate presence of proteins from the co-cultured microbiota of N. perurans was revealed on searching an amalgamated database of bacterial, N. perurans and Amoebozoa proteins. LC‑MS/MS identified 33 differentially expressed proteins, the majority of which were upregulated in the attenuated exoproteome. Proteins of putative interest found in both exoproteomes were maltoporin, ferrichrome-iron receptor, and putative ferric enterobactin receptor. Protease activity remained significantly elevated in the attenuated exoproteome compared with the virulent exoproteome. Similarly, the attenuated exoproteome had a significantly higher cytotoxic effect on rainbow trout gill cell line (RTgill W1) cells compared with the virulent exoproteome. The presence of a phosphatase and serine protease in the virulent exoproteome may facilitate AGD infection but do not appear to be key players in causing cytotoxicity. Altogether, this study reveals prolonged culture of N. perurans affects the exoproteome composition in favour of nutritional acquisition, and that the current culturing protocol for virulent N. perurans does not facilitate the secretion of virulence factors.

Responses of trout gill ion transport systems to acute acidosis

1976 ◽  
Vol 64 (2) ◽  
pp. 511-515
Author(s):  
T. H. Kerstetter ◽  
R. Mize
Keyword(s):  
Lactic Acid ◽  
Rainbow Trout ◽  
Ion Transport ◽  
Ion Uptake ◽  
Transport Systems ◽  
Ph Changes ◽  
Blood Ph ◽  
Slow Infusion ◽  
Trout Gill

The response of rainbow trout Na+ and Cl- uptake systems to acute acidosis was tested by slow infusion of lactic acid into anaesthetized animals. Depression of blood pH by 0–4 pH unit had no effect on influx rates for either ion, and we conclude that gill ion uptake systems do not respond rapidly to blood pH changes.

scholarly journals Procedures for the reconstruction, primary culture and experimental use of rainbow trout gill epithelia

2016 ◽  
Vol 11 (3) ◽  
pp. 490-498 ◽  
Author(s):  
Sabine Schnell ◽  
Lucy C Stott ◽  
Christer Hogstrand ◽  
Chris M Wood ◽  
Scott P Kelly ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
Primary Culture ◽  
Experimental Use ◽  
Trout Gill

Effects of copper on CYP1A activity and epithelial barrier properties in the rainbow trout gill

2006 ◽  
Vol 79 (1) ◽  
pp. 78-86 ◽  
Author(s):  
Maria E. Jönsson ◽  
Carina Carlsson ◽  
Richard W. Smith ◽  
Peter Pärt
Keyword(s):  
Rainbow Trout ◽  
Barrier Properties ◽  
Epithelial Barrier ◽  
Cyp1a Activity ◽  
Trout Gill

scholarly journals On the Mechanism of Sodium Extrusion across the Irrigated Gill of Sea Water-Adapted Rainbow Trout (Salmo gairdneri)

1974 ◽  
Vol 64 (2) ◽  
pp. 148-165 ◽  
Author(s):  
Leonard B. Kirschner ◽  
Lewis Greenwald ◽  
Martin Sanders
Keyword(s):  
Rainbow Trout ◽  
Active Component ◽  
Sea Water ◽  
Salmo Gairdneri ◽  
Sodium Efflux ◽  
Exchange Diffusion ◽  
The Difference ◽  
Sodium Extrusion ◽  
Trout Gill ◽  
Electrical Data

Sodium efflux (JoutNa) across the irrigated trout gill was rapid in sea water (SW), but only about 25 % as large in fresh water (FW). The difference correlated with a change in the potential difference across the gill (TEP). The latter was about +10 mV (blood positive) in SW, but –40 mV in FW. Both flux and electrical data indicated that gills in this fish are permeable to a variety of cations including Na+, K+, Mg2+, choline, and Tris. They are less permeable to anions; PNa:PK:PCl was estimated to be 1:10:0.3, and PCl > Pgluconate. The TEP was shown to be a diffusion potential determined by these permeabilities and the extant ionic gradients in SW, FW as well as in other media. JoutNa appeared to be diffusive in all of the experiments undertaken. Exchange diffusion need not be posited, and the question of whether there is an active component remains open.

scholarly journals Immunolocalization of proton pumps (H+-ATPase) in pavement cells of rainbow trout gill

1995 ◽  
Vol 198 (12) ◽  
pp. 2619-2629 ◽  
Author(s):  
G Sullivan ◽  
J Fryer ◽  
S Perry
Keyword(s):  
Electron Microscopy ◽  
Rainbow Trout ◽  
Epithelial Cells ◽  
Apical Membrane ◽  
Respiratory Acidosis ◽  
Proton Pumps ◽  
Ion Regulation ◽  
Western Blots ◽  
Pavement Cells ◽  
Trout Gill

The expression of the V-type proton ATPase (H+-ATPase) was examined in the gill of the freshwater rainbow trout (Oncorhynchus mykiss) using immunocytochemistry in concert with laser scanning confocal or electron microscopy. A synthetic peptide consisting of the carboxy-terminal region of the 31 kDa subunit of the bovine renal H+-ATPase was used to generate an antiserum in rabbits, and its suitability for use in trout gill was confirmed by western blotting. Gill epithelial cells demonstrated specific immunoreactivity, the intensity of which was increased markedly after 18 h of exposure to hypercapnia (1 % CO2 in air). The increased intensity of H+-ATPase immunoreactivity was associated with elevated branchial net acid excretion. In the hypercapnic fish, the specific immunoreactivity was associated with both the apical membrane and cytoplasm. Electron microscopy revealed that specific immunoreactivity was localized to the pavement cells and was particularly associated with the apical membrane and subapical cytoplasmic vesicles. The increased H+-ATPase immunoreactivity in the epithelial cells of hypercapnic fish and the increased intensity of the immunoreactive bands in western blots from hypercapnic fish demonstrate an 'up-regulation' of this protein in response to respiratory acidosis. The results are discussed with reference to current models of acid­base and ion regulation in the gill of freshwater fish.

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