scholarly journals Unicellular versus Filamentous: The Glacial Alga Ancylonema alaskana comb. et stat. nov. and Its Ecophysiological Relatedness to Ancylonema nordenskioeldii (Zygnematophyceae, Streptophyta)

2021 ◽  
Vol 9 (5) ◽  
pp. 1103
Author(s):  
Lenka Procházková ◽  
Tomáš Řezanka ◽  
Linda Nedbalová ◽  
Daniel Remias
Keyword(s):  
Fatty Acids ◽  
Molecular Analysis ◽  
Surface Albedo ◽  
Pulse Amplitude ◽  
Sensu Stricto ◽  
Solar Irradiation ◽  
High Tolerance ◽  
Pam Fluorometry ◽  
Cellular Fatty Acids ◽  
Original Habitat

Melting polar and alpine ice surfaces frequently exhibit blooms of dark pigmented algae. These microbial extremophiles significantly reduce the surface albedo of glaciers, thus accelerating melt rates. However, the ecology, physiology and taxonomy of cryoflora are not yet fully understood. Here, a Swiss and an Austrian glacier dominated either by filamentous Ancylonema nordenskioeldii or unicellular Mesotaenium berggrenii var. alaskanum, were sampled. Molecular analysis showed that both species are closely related, sharing identical chloroplast morphologies (parietal-lobed for Ancylonema vs. axial plate-like for Mesotaenium sensu stricto), thus the unicellular species was renamed Ancylonema alaskana. Moreover, an ecophysiological comparison of the two species was performed: pulse–amplitude modulated (PAM) fluorometry confirmed that they have a high tolerance to elevated solar irradiation, the physiological light preferences reflected the conditions in the original habitat; nonetheless, A. nordenskioeldii was adapted to higher irradiances while the photosystems of A. alaskana were able to use efficiently low irradiances. Additionally, the main vacuolar polyphenol, which effectively shields the photosystems, was identical in both species. Also, about half of the cellular fatty acids were polyunsaturated, and the lipidome profiles dominated by triacylglycerols were very similar. The results indicate that A. alaskana is physiologically very similar and closely related but genetically distinct to A. nordenskioeldii.

Cellular fatty acids derived from normal alkanes byCandida rugosa

1980 ◽  
Vol 20 (7) ◽  
pp. 449-457 ◽  
Author(s):  
M. Iida ◽  
H. Kobayashi ◽  
H. Iizuka
Keyword(s):  
Fatty Acids ◽  
Normal Alkanes ◽  
Cellular Fatty Acids

scholarly journals A Novel Thin-Film Technique to Improve Accuracy of Fluorescence-Based Estimates for Periphytic Biofilms

Water ◽  
2021 ◽  
Vol 13 (11) ◽  
pp. 1464
Author(s):  
Leon Katona ◽  
Yvonne Vadeboncoeur ◽  
Christopher T. Nietch ◽  
Katie Hossler
Keyword(s):  
Thin Film ◽  
Thin Films ◽  
Light Attenuation ◽  
Pulse Amplitude ◽  
Light Signal ◽  
Photosynthetic Parameters ◽  
Pam Fluorometry ◽  
Attenuation Coefficients ◽  
Thin Film Technique ◽  
Relative Errors

Recent studies suggest that photophysiological parameters for intact substrates with depth (e.g., periphytic biofilms, microphytobenthos) are overestimated by pulse-amplitude modulated (PAM) fluorometry. This overestimation results from depth-integration effects, following the activation of deeper photosynthesizing layers by an attenuated light signal. To mitigate this error, we propose a novel slide-based thin-film technique in which fluorescence is measured on a vertically representative subsample of the biofilm, spread evenly on a microscope slide. We compared bias and precision for photosynthetic parameters estimated through conventional PAM fluorometry on intact biofilms and through our novel slide-based technique, both theoretically and empirically. Numerical simulations confirmed the consistent overestimation of key parameters for intact biofilms, with relative errors up to 145%, compared to, at most, 52% on thin films. Paired empirical observations likewise demonstrated that estimates based on intact biofilms were consistently higher (up to 248%, p<0.001) than estimates from thin films. Numerical simulation suggested greater precision with the slide-based technique for homogeneous biofilms, but potentially less precision for heterogeneous biofilms with improper subsampling. Our empirical comparison, however, demonstrated some improvement in precision with the slide-based technique (e.g., the coefficient of variation for the maximum electron transport rate was reduced 30%, p=0.009). We recommend the use of the slide-based technique, particularly for biofilms that are thick or have small light attenuation coefficients. Care should be taken, however, to obtain vertically representative subsamples of the biofilm for measurement.

scholarly journals Intraspecies cellular fatty acids heterogeneity ofLactobacillus plantarumstrains isolated from fermented foods in Ukraine

2015 ◽  
Vol 61 (3) ◽  
pp. 283-292 ◽  
Author(s):  
I. Garmasheva ◽  
O. Vasyliuk ◽  
N. Kovalenko ◽  
A. Ostapchuk ◽  
L. Oleschenko
Keyword(s):  
Fatty Acids ◽  
Fermented Foods ◽  
Cellular Fatty Acids

Caldicellulosiruptor diazotrophicus sp. nov., a thermophilic, nitrogen-fixing fermentative bacterium isolated from a terrestrial hot spring in Japan

2021 ◽  
Vol 71 (9) ◽  
Author(s):  
Yuxin Chen ◽  
Arisa Nishihara ◽  
Takao Iino ◽  
Moriya Ohkuma ◽  
Shin Haruta
Keyword(s):  
Fatty Acids ◽  
16S Rrna ◽  
Type Species ◽  
Gene Sequence ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Content Type ◽  
Cellular Fatty Acids ◽  
Link Type ◽  
The 16S Rrna Gene

A novel nitrogen-fixing fermentative bacterium, designated as YA01T, was isolated from Nakabusa hot springs in Japan. The short-rod cells of strain YA01T were Gram-positive and non-sporulating. Phylogenetic trees of the 16S rRNA gene sequence and concatenated sequences of 40 single-copy ribosomal genes revealed that strain YA01T belonged to the genus Caldicellulosiruptor and was closely related to Caldicellulosiruptor hydrothermalis 108T, Caldicellulosiruptor bescii DSM 6725T and Caldicellulosiruptor kronotskyensis 2002T. The 16S rRNA gene sequence of strain YA01T shares less than 98.1 % identity to the known Caldicellulosiruptor species. The G+C content of the genomic DNA was 34.8 mol%. Strain YA01T shares low genome-wide average nucleotide identity (90.31–91.10 %), average amino acid identity (91.45–92.10 %) and <70 % digital DNA–DNA hybridization value (41.8–44.2 %) with the three related species of the genus Caldicellulosiruptor . Strain YA01T grew at 50–78 °C (optimum, 70 °C) and at pH 5.0–9.5 (optimum, pH 6.5). Strain YA01T mainly produced acetate by consuming d(+)-glucose as a carbon source. The main cellular fatty acids were iso-C17 : 0 (35.7 %), C16 : 0 (33.3 %), DMA16 : 0 (6.6 %) and iso-C15 : 0 (5.9 %). Based on its distinct phylogenetic position, biochemical and physiological characteristics, and the major cellular fatty acids, strain YA01T is considered to represent a novel species of the genus Caldicellulosiruptor for which the name Caldicellulosiruptor diazotrophicus sp. nov. is proposed (type strain YA01T=DSM 112098T=JCM 34253T).

Cellular fatty acids of Peptococcus variabilis and Peptostreptococcus anaerobius

1977 ◽  
Vol 5 (6) ◽  
pp. 665-667
Author(s):  
C W Moss ◽  
M A Lambert ◽  
G L Lombard
Keyword(s):  
Mass Spectrometry ◽  
Fatty Acids ◽  
Gas Chromatography ◽  
Analytical Techniques ◽  
Gas Chromatography Mass Spectrometry ◽  
Cellular Fatty Acids ◽  
Chromatography Mass Spectrometry ◽  
Branched Chain

Cellular fatty acids of Peptococcus variables and Peptostreptococcus anaerobius were identified by gas chromatography, mass spectrometry, and associated analytical techniques. Iso- and anteiso-branched-chain acids were major components in both species.

Cellular Fatty Acids of Streptobacteria Isolated from Vacuum Packaged Meats

1984 ◽  
Vol 5 (2) ◽  
pp. 233-240 ◽  
Author(s):  
Richard H. Dainty ◽  
Christine M. Hibbard ◽  
Robert A. Edwards
Keyword(s):  
Fatty Acids ◽  
Cellular Fatty Acids

Multivariate analyses of cellular fatty acids and carbohydrates of 1:2:1 and 2:4:2 spirochetes

Apmis ◽  
1991 ◽  
Vol 99 (1-6) ◽  
pp. 567-575 ◽  
Author(s):  
ILIA BRONDZ ◽  
NILS-ERIK FIEHN ◽  
INGAR OLSEN ◽  
MICHAEL SJÖSTRÖM
Keyword(s):  
Fatty Acids ◽  
Multivariate Analyses ◽  
Cellular Fatty Acids
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