scholarly journals Detection of KPC, NDM and VIM-Producing Organisms Directly from Rectal Swabs by a Multiplex Lateral Flow Immunoassay

2021 ◽  
Vol 9 (5) ◽  
pp. 942
Author(s):  
Alexandra Vasilakopoulou ◽  
Polyxeni Karakosta ◽  
Sophia Vourli ◽  
Eleni Kalogeropoulou ◽  
Spyros Pournaras
Keyword(s):  
Antimicrobial Treatment ◽  
Immunochromatographic Assay ◽  
Lateral Flow Immunoassay ◽  
Preliminary Evaluation ◽  
Reliable Test ◽  
Time Points ◽  
Conventional Culture ◽  
Direct Testing ◽  
Rectal Swabs ◽  
Fecal Content

We report a preliminary evaluation of the NG-Test CARBA 5 immunochromatographic assay for detecting carbapenemases directly from rectal swabs on the same day of sampling. Thirty fecal swabs were examined for carbapenemase-producing organisms (CPOs) by conventional culture, PCR, and NG-Test CARBA 5. Each sample was tested by the immunochromatographic assay five times, including direct testing and incubation in trypticase soy broth for 1, 2, 3, and 4 h. Twenty patients yielded CPOs by culture. Immunochromatographic and PCR results were concordant and detected the same 25 carbapenemases (11 KPC, 8 VIM, and 6 NDM). In five cases, we detected co-carriage of KPC and VIM. Compared with PCR, the sensitivity of NG-Test CARBA 5 for the detection of KPC, VIM, and NDM was 80% without incubation, 88% with one hour, 92% with two, and 100% with three hours incubation, while specificity was 100% for all time points. All samples containing adequate fecal content were detected by NG-Test CARBA 5 concordantly with PCR, without incubation. NG-Test CARBA 5 is a reliable test that rapidly detects the presence of carbapenemases at the same day of sampling, directly from rectal swabs. It thus provides early information to guide antimicrobial treatment and infection control interventions.

Preliminary Evaluation of Knee Kinetics in Female Athletes on Hormonal Contraceptives

2019 ◽  
Vol 41 (02) ◽  
pp. 113-118
Author(s):  
Gabrielle Gilmer ◽  
Gretchen D. Oliver
Keyword(s):  
Injury Risk ◽  
Female Athletes ◽  
Cruciate Ligament ◽  
Reaction Force ◽  
Acl Injury ◽  
Hormonal Contraceptives ◽  
Preliminary Evaluation ◽  
Knee Valgus ◽  
Time Points ◽  
Acl Injury Risk

AbstractRecently, an emphasis has been placed on understanding how ovarian sex hormones and hormonal contraceptives affect risk for anterior cruciate ligament (ACL) injury. The literature presents large discrepancies in whether or not hormonal contraceptives affect ACL injury risk; therefore, the purpose of this study was to evaluate whether vertical ground reaction force (GRF) and knee valgus force are different between athletes who do and do not use hormonal contraceptives. Twenty-two female athletes volunteered to participate and were divided into two groups based on their answers to a health history questionnaire: those who use hormonal contraceptives and those who do not. Participants performed a drop vertical jump (DVJ) and single leg crossover dropdown (SCD) at two different time points in their menstrual cycle (pre-ovulatory phase and mid-luteal phase). Kinetic data were collected at 1000 Hz. Independent samples t-tests revealed no significant differences between groups in vertical GRF and knee valgus force at both time points. Findings from this study suggest that hormonal contraceptives do not elicit detectable changes in vertical GRF and knee valgus force. Ultimately, this calls for further studies on the relationship between hormones and ACL injury risk and physicians to consider hormonal screening in addition to neuromuscular and biomechanical screening.

Preliminary evaluation of a lateral flow immunoassay device for screening urine samples for the presence of sulphamethazine

2003 ◽  
Vol 278 (1-2) ◽  
pp. 117-126 ◽  
Author(s):  
M O'Keeffe ◽  
P Crabbe ◽  
M Salden ◽  
J Wichers ◽  
C Van Peteghem ◽  
...  
Keyword(s):  
Lateral Flow ◽  
Urine Samples ◽  
Lateral Flow Immunoassay ◽  
Preliminary Evaluation

scholarly journals Performance of Commercially Available Rapid Serological Assays for The Detection of SARS-CoV-2 Antibodies

2020 ◽  
Author(s):  
Anwar M Hashem ◽  
Rowa Y Y Rowa Y Alhabbab ◽  
Abdullah Algaissi ◽  
Mohamed A Alfaleh ◽  
Sharif Hala ◽  
...  
Keyword(s):  
Severe Acute Respiratory Syndrome ◽  
Significant Variation ◽  
High Specificity ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Specific Antibodies ◽  
Time Points ◽  
Igm Antibodies ◽  
Specific Igg ◽  
High Degree

Abstract Background: The Coronavirus Disease 2019 (COVID-19) pandemic, caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), continues to spread globally. Although several commercial SARS-CoV-2 rapid serological assays have been developed, little is known about their performance and accuracy in detecting SARS-CoV-2 specific antibodies in COVID-19 patient samples. Method: We have evaluated the performance of seven commercially available rapid lateral flow immunoassay (LFIA) serological assays obtained from different manufacturers, and compared them to in-house developed and validated ELISA assays for the detection of SARS-CoV-2 specific IgG and IgM antibodies in COVID-19 patients. Results: While all evaluated LFIA assays showed high specificity, our data showed a significant variation in sensitivity of these assays in which it ranged from 0 to 54% for samples collected early during infection (3-7 days post symptoms onset) and from 54 to 88% for samples collected at later time points during infection (8-27 days post symptoms onset). Conclusion: Commercially available LFIA assays for detection of SARS-CoV-2 specific antibodies may be specific and show high degree of variation in their sensitivity. Further evaluations and validation of rapid serological assays is needed before being routinely used in detecting IgM and IgG in COVID-19 patients.

Detection of OXA-370 directly from rectal swabs and blood culture vials using an immunochromatographic assay

2017 ◽  
Vol 139 ◽  
pp. 92-94 ◽  
Author(s):  
Carolina Silva Nodari ◽  
Ana Cristina Gales ◽  
Afonso Luís Barth ◽  
Cibele Massotti Magagnin ◽  
Alexandre Prehn Zavascki ◽  
...  
Keyword(s):  
Blood Culture ◽  
Immunochromatographic Assay ◽  
Rectal Swabs

Multiplex lateral flow immunoassay for five antibiotics detection based on gold nanoparticle aggregations

RSC Advances ◽  
2016 ◽  
Vol 6 (10) ◽  
pp. 7798-7805 ◽  
Author(s):  
Juan Peng ◽  
Yongwei Wang ◽  
Liqiang Liu ◽  
Hua Kuang ◽  
Aike Li ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Gold Nanoparticle ◽  
Lateral Flow ◽  
Immunochromatographic Assay ◽  
Lateral Flow Immunoassay ◽  
Antibiotics Detection

A new immunochromatographic assay was developed for the simultaneous screening of five antibiotics that can coexist in milk, namely lincomycin, gentamicin, kanamycin, streptomycin, and neomycin, using five corresponding monoclonal antibodies.

Preliminary Evaluation of Flow-Through Immunocapture followed by Real-Time PCR for the Detection of Salmonella Serovars on Tomato Surfaces within 8 Hours

2006 ◽  
Vol 69 (9) ◽  
pp. 2253-2257 ◽  
Author(s):  
HYUN-GYUN YUK ◽  
BENJAMIN R. WARREN ◽  
KEITH R. SCHNEIDER
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Culture Method ◽  
Preliminary Evaluation ◽  
Conventional Culture ◽  
Significant Difference ◽  
Assay Time ◽  
Flow Through ◽  
Pcr Method ◽  
Salmonella Serovars

This study reports a preliminary evaluation of flow-through immunocapture (FTI) followed by real-time PCR (FTI-PCR) for the detection of Salmonella serovars on tomato surfaces within 8 h. The FTI-PCR method was compared with real-time PCR, direct plating of FTI beads on xylose lysine desoxycholate (XLD), and the conventional culture method for Salmonella found in the U.S. Food and Drug Administration's (FDA) Bacteriological Analytical Manual (BAM). Unwaxed green tomatoes were spot inoculated with a five-serovar Salmonella cocktail on smooth surfaces at levels of 100 to 104 CFU per tomato and washed in lactose broth (LB) using a shake-rub method. The resulting LB rinse was incubated at 37°C for 4 h prior to analysis by FTI-XLD, real-time PCR, or FTI-PCR and for 24 h as the first step in the BAM Salmonella culture method. For FTI-XLD, the observed lowest detection level (LDL) was 4.6 × 101 CFU per tomato. There was no significant difference in performance between the FTI-XLD method and the BAM Salmonella culture method (P > 0.05); however, the FTI-XLD method reduced the overall assay time by 48 h. For real-time PCR and FTI-PCR, the observed LDLs were 4.6 × 101 and 9.2 × 100 CFU per tomato, respectively. The FTI-PCR method was superior to the BAM Salmonella culture method (P < 0.05) for the detection of Salmonella serovars on tomato surfaces and was completed within 8 h.

scholarly journals Rectal Swabs from Critically Ill Patients Provide Discordant Representations of the Gut Microbiome Compared to Stool Samples

mSphere ◽  
2019 ◽  
Vol 4 (4) ◽  
Author(s):  
Katherine Fair ◽  
Daniel G. Dunlap ◽  
Adam Fitch ◽  
Tatiana Bogdanovich ◽  
Barbara Methé ◽  
...  
Keyword(s):  
Critical Illness ◽  
Critically Ill ◽  
Critically Ill Patients ◽  
Gut Microbiome ◽  
Rectal Swab ◽  
Time Points ◽  
Rectal Swabs ◽  
Stool Samples ◽  
Microbiome Profiling

ABSTRACT The role of the gut microbiome in critical illness is being actively investigated, but the optimal sampling methods for sequencing studies of gut microbiota remain unknown. Stool samples are generally considered the reference standard but are not practical to obtain in the intensive care unit (ICU), and thus, rectal swabs are often used. However, the reliability of rectal swabs for gut microbiome profiling has not been established in the ICU setting. In this study, we compared 16S rRNA gene sequencing results between rectal swab and stool samples collected at three time points from mechanically ventilated critically ill adults. Rectal swabs comprised 89% of the samples collected at the baseline time point, but stool samples became more extensively available at later time points. Significant differences in alpha-diversity and beta-diversity between rectal swabs and stool samples were observed, but these differences were primarily due to baseline samples. Higher relative abundances of members of the Actinobacteria phylum (typically skin microbes) were present in rectal swabs than in stool samples (P = 0.05), a difference that was attenuated over time. The progressively increasing similarity of rectal swabs and stool samples likely resulted from increasing levels of stool coating of the rectal vault and direct soiling of the rectal swabs taken at later time points. Therefore, inferences about the role of the gut microbiome in critical illness should be drawn cautiously and should take into account the type and timing of samples analyzed. IMPORTANCE Rectal swabs have been proposed as potential alternatives to stool samples for gut microbiome profiling in outpatients or healthy adults, but their reliability in assessment of critically ill patients has not been defined. Because stool sampling is not practical and often not feasible in the intensive care unit, we performed a detailed comparison of gut microbial sequencing profiles between rectal swabs and stool samples in a longitudinal cohort of critically ill patients. We identified systematic differences in gut microbial profiles between rectal swabs and stool samples and demonstrated that the timing of the rectal swab sampling had a significant impact on sequencing results. Our methodological findings should provide valuable information for the design and interpretation of future investigations of the role of the gut microbiome in critical illness.

Algorithms for immunochromatographic assay: review and impact on future application

The Analyst ◽  
2019 ◽  
Vol 144 (19) ◽  
pp. 5659-5676 ◽  
Author(s):  
Qi Qin ◽  
Kan Wang ◽  
Jinchuan Yang ◽  
Hao Xu ◽  
Bo Cao ◽  
...  
Keyword(s):  
Artificial Intelligence ◽  
Deep Learning ◽  
Lateral Flow ◽  
Immunochromatographic Assay ◽  
Future Application ◽  
Lateral Flow Immunoassay

This review summarizes different models for the lateral flow immunoassay technology when combined with artificial intelligence and deep learning.

scholarly journals Rectal swabs in critically-ill patients provide discordant representations of the gut microbiome compared to stool samples: a brief methodologic report

2019 ◽  
Author(s):  
Katherine Fair ◽  
Daniel G. Dunlap ◽  
Adam Fitch ◽  
Alison Morris ◽  
Bryan J. McVerry ◽  
...  
Keyword(s):  
Intensive Care Unit ◽  
Critical Illness ◽  
Critically Ill ◽  
Critically Ill Patients ◽  
Gut Microbiome ◽  
Time Points ◽  
Rectal Swabs ◽  
Stool Samples ◽  
Microbiome Profiling

AbstractThe role of the gut microbiome in critical illness is being actively investigated, but the optimal sampling methods for sequencing studies of gut microbiota remain unknown. Stool samples are generally considered gold-standard but are not practical to obtain in the intensive care unit (ICU), and thus, rectal swabs are often used. However, the reliability of rectal swabs for gut microbiome profiling has not been established in this clinical setting. In this study, we compared 16S rRNA gene sequencing results between rectal swab and stool samples collected at three timepoints in mechanically-ventilated critically-ill adults. Rectal swabs comprised 89% of samples collected at the baseline timepoint, but stool samples became more available at later time-points. Significant differences in alpha and beta-diversity between rectal swabs and stool samples were observed (p<0.003), but these differences were primarily due to baseline samples. Higher relative abundance of Actinobacteria phyla (typically skin microbes) was present in rectal swabs compared to stool samples (p<0.02), a difference that was attenuated overtime. The progressive similarity of rectal swabs and stool samples likely results from increasing stool coating of the rectal vault and direct soiling of the rectal swabs taken at later time points. Therefore, inferences about the role of the gut microbiome in critical illness should be drawn cautiously and take into account the actual type and timing of samples analyzed.Statement of ImportanceRectal swabs are considered reliable alternatives to stool samples for gut microbiome profiling in outpatients or healthy adults, but their reliability in critically-ill patients has not been defined. Because stool sampling is not practical or often feasible in the intensive care unit, we performed a detailed comparison of gut microbial sequencing profiles between rectal swabs vs. stool samples in a longitudinal cohort of critically-ill patients. We identified systematic differences in gut microbial profiles between rectal swabs and stool samples, and highlighted that timing of rectal swabbing had a significant impact on sequencing results. Our methodological findings should inform future investigations of the role of the gut microbiome in critical illness.

scholarly journals An optimized work-flow to reduce time-to-detection of carbapenemase-producing Enterobacteriaceae (CPE) using direct testing from rectal swabs

Bioengineered ◽  
2016 ◽  
Vol 8 (3) ◽  
pp. 217-224 ◽  
Author(s):  
C. O'Connor ◽  
M. G. Kiernan ◽  
C. Finnegan ◽  
M. O'Hara ◽  
L. Power ◽  
...  
Keyword(s):  
Work Flow ◽  
Direct Testing ◽  
Rectal Swabs ◽  
Time To Detection
Sign in / Sign up

Export Citation Format

Share Document